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- SASDBH2: Mutant archaeal biofilm regulator 1 (ABfR1) phosphomimic Y84E S87D -

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Basic information

Entry
Database: SASBDB / ID: SASDBH2
SampleMutant archaeal biofilm regulator 1 (ABfR1) phosphomimic Y84E S87D
  • Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant (protein), ABfR1 mutant Y84E S8, Sulfolobus acidocaldarius
Function / homologyTranscription regulator TrmB, N-terminal / Sugar-specific transcriptional regulator TrmB / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily / Transcriptional regulator ArsR family
Function and homology information
Biological speciesSulfolobus acidocaldarius (acidophilic)
Contact author
  • Ankan Banerjee (Philipps-Universität Marburg, Marburg, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #399
Type: dummy / Radius of dummy atoms: 2.25 A / Chi-square value: 0.957
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Mutant archaeal biofilm regulator 1 (ABfR1) phosphomimic Y84E S87D
Specimen concentration: 3.8 mg/ml
BufferName: 20 mM HEPES 200 mM NaCl / Concentration: 20.00 mM / pH: 7.5 / Composition: 200 mM NaCl
Entity #264Name: ABfR1 mutant Y84E S8 / Type: protein
Description: Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant
Formula weight: 13.16 / Num. of mol.: 2 / Source: Sulfolobus acidocaldarius / References: UniProt: Q4JBH1
Sequence:
MSIEITEKYV ILKRFLSVAY GLSESEIDTF LRILESKEGK DIDAISAELK ISKSRTSIIL KKLSDAGLVD KEKGDNMKGG RPKEVYDVNK EELKNRLIAK SEELCKELNQ VIKSFI

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.93 Å / Dist. spec. to detc.: 2.43 mm
DetectorName: Pilatus 1M
Scan
Title: Archaeal biofilm regulator 1 (AbfR1) Y84E S87D / Measurement date: Aug 27, 2015 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 20 sec. / Number of frames: 10 / Unit: 1/nm /
MinMax
Q0.0327 4.9336
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 783 /
MinMax
Q0.112644 3.79068
P(R) point1 783
R0 9.5
Result
Type of curve: single_conc
ExperimentalStandardPorod
MW32.3 kDa32.25 kDa33.3 kDa
Volume--50.93 nm3

P(R)Guinier
Forward scattering, I032.3 32.2
Radius of gyration, Rg2.69 nm2.64 nm

MinMax
D-9.5
Guinier point6 96

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