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- SASDB65: Tyrosine-protein phosphatase (YopH)/putative yopH targeting prote... -

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Basic information

Entry
Database: SASBDB / ID: SASDB65
SampleTyrosine-protein phosphatase (YopH)/putative yopH targeting protein (SycH) heterotrimeric complex
  • SycH putative yopH targeting protein (protein), SycH, Yersinia pseudotuberculosis
  • Tyrosine-protein phosphatase YopH (protein), YopH, Yersinia pseudotuberculosis
Function / homology
Function and homology information


protein secretion by the type III secretion system / dephosphorylation / protein-tyrosine-phosphatase / protein tyrosine phosphatase activity / extracellular region
Similarity search - Function
Tir chaperone protein (CesT) family / Tir chaperone protein (CesT) family / Protein-tyrosine phosphatase, YopH, N-terminal / Protein-tyrosine phosphatase, YopH, N-terminal domain superfamily / YopH, N-terminal / Type III secreted modular tyrosine phosphatase, SptP/YopH / Protein tyrosine phosphatase, catalytic domain / PTP type protein phosphatase domain profile. / Protein-tyrosine phosphatase / Tyrosine-specific protein phosphatase, PTPase domain ...Tir chaperone protein (CesT) family / Tir chaperone protein (CesT) family / Protein-tyrosine phosphatase, YopH, N-terminal / Protein-tyrosine phosphatase, YopH, N-terminal domain superfamily / YopH, N-terminal / Type III secreted modular tyrosine phosphatase, SptP/YopH / Protein tyrosine phosphatase, catalytic domain / PTP type protein phosphatase domain profile. / Protein-tyrosine phosphatase / Tyrosine-specific protein phosphatase, PTPase domain / Protein-tyrosine phosphatase, catalytic / Protein tyrosine phosphatase, catalytic domain motif / Tyrosine specific protein phosphatases active site. / Protein-tyrosine phosphatase, active site / Tyrosine-specific protein phosphatases domain / Tyrosine specific protein phosphatases domain profile. / Protein-tyrosine phosphatase-like
Similarity search - Domain/homology
SycH putative yopH targeting protein / Tyrosine-protein phosphatase YopH
Similarity search - Component
Biological speciesYersinia pseudotuberculosis (bacteria)
CitationDate: 2017 Feb
Title: Global Disordering in Stereo-Specific Protein Association
Authors: Gupta A / Reinartz I / Spilotros A / Jonna V / Hofer A / Svergun D / Schug A
Contact author
  • Alessandro Spilotros (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #556
Type: atomic / Radius of dummy atoms: 1.90 A / Chi-square value: 1.271
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Tyrosine-protein phosphatase (YopH)/putative yopH targeting protein (SycH) heterotrimeric complex
Specimen concentration: 1 mg/ml / Entity id: 356 / 357
BufferName: 50 mM HEPES 2mM TCEP / Concentration: 50.00 mM / pH: 6.8 / Composition: 2mM TCEP
Entity #356Name: SycH / Type: protein / Description: SycH putative yopH targeting protein / Formula weight: 15.763 / Num. of mol.: 2 / Source: Yersinia pseudotuberculosis / References: UniProt: I3NIC8
Sequence:
MRTYSSLLEE FATELGLEEI ETNELGHGAV TIDKIWVVHL APINEKELVA FMRAGILTGQ SQLYDILRKN LFSPLSGVIR CALDKDDHWL LWSQLNINDT SGTQLASVLT SLVDKAVTLS CEPTMKKEED DHRPSSSHLL V
Entity #357Name: YopH / Type: protein / Description: Tyrosine-protein phosphatase YopH / Formula weight: 13.95 / Num. of mol.: 1 / Source: Yersinia pseudotuberculosis / References: UniProt: P08538
Sequence:
MNLSLSDLHR QVSRLVQQES GDCTGKLRGN VAANKETTFQ GLTIASGARE SEKVFAQTVL SHVANVVLTQ EDTAKLLQST VKHNLNNYDL RSVGNGNSVL VSLRSDQMTL QDAKVLLEAA LRQESGARG

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Experimental information

BeamInstrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.12 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 2M
Scan
Title: YopH/SycH heterotrimeric complex / Measurement date: Aug 1, 2016 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.0664 4.8081
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1266 /
MinMax
Q0.0901183 3.4299
P(R) point1 1266
R0 12.5
Result
Type of curve: single_conc /
ExperimentalPorod
MW52 kDa55.8 kDa
Volume-89.2 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I074550 130 74475 117
Radius of gyration, Rg3.01 nm0.013 2.96 nm0.13

MinMax
D-12.5
Guinier point22 115

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