SASDAZ4: fH1114 (Factor H CCP modules 11 to 14)

基本情報

• 登録情報: データベース: SASBDB / ID: SASDAZ4

試料

fH1114

• Factor H CCP modules 11 to 14 (protein), fH1114, Homo sapiens

• 生物種: Homo sapiens (ヒト)
• 引用: ジャーナル: J Mol Biol / 年: 2010; タイトル: The central portion of factor H (modules 10-15) is compact and contains a structurally deviant CCP module.; 著者: Christoph Q Schmidt / Andrew P Herbert / Haydyn D T Mertens / Mara Guariento / Dinesh C Soares / Dusan Uhrin / Arthur J Rowe / Dmitri I Svergun / Paul N Barlow / ; 要旨: The first eight and the last two of 20 complement control protein (CCP) modules within complement factor H (fH) encompass binding sites for C3b and polyanionic carbohydrates. These binding sites cooperate self-surface selectively to prevent C3b amplification, thus minimising complement-mediated damage to host. Intervening fH CCPs, apparently devoid of such recognition sites, are proposed to play a structural role. One suggestion is that the generally small CCPs 10-15, connected by longer-than-average linkers, act as a flexible tether between the two functional ends of fH; another is that the long linkers induce a 180 degrees bend in the middle of fH. To test these hypotheses, we determined the NMR-derived structure of fH12-13 consisting of module 12, shown here to have an archetypal CCP structure, and module 13, which is uniquely short and features a laterally protruding helix-like insertion that contributes to a prominent electropositive patch. The unusually long fH12-13 linker is not flexible. It packs between the two CCPs that are not folded back on each other but form a shallow vee shape; analytical ultracentrifugation and X-ray scattering supported this finding. These two techniques additionally indicate that flanking modules (within fH11-14 and fH10-15) are at least as rigid and tilted relative to neighbours as are CCPs 12 and 13 with respect to one another. Tilts between successive modules are not unidirectional; their principal axes trace a zigzag path. In one of two arrangements for CCPs 10-15 that fit well with scattering data, CCP 14 is folded back onto CCP 13. In conclusion, fH10-15 forms neither a flexible tether nor a smooth bend. Rather, it is compact and has embedded within it a CCP module (CCP 13) that appears to be highly specialised given both its deviant structure and its striking surface charge distribution. A passive, purely structural role for this central portion of fH is unlikely.

登録者

• Haydyn Mertens (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

モデル

• モデル #84: ; タイプ: dummy / ソフトウェア: dammif / ダミー原子の半径: 1.90 A / カイ2乗値: 2.0449; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #85: ; タイプ: dummy / ソフトウェア: gasbor / ダミー原子の半径: 1.90 A / コメント: gasbor model / カイ2乗値: 2.1025; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #86: ; タイプ: mix / ソフトウェア: bunch / ダミー原子の半径: 1.90 A / カイ2乗値: 1.452025; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: fH1114 / Sample MW: 27.6 kDa / 試料濃度: 0.70-1.60 / 濃度測定法: A280
• バッファ: 名称: Potassium Phosphate / 濃度: 50.00 mM / pH: 7.4

要素 #72

名称: fH1114 / タイプ: protein / 記述: Factor H CCP modules 11 to 14 / 分子量: 27.6 / 分子数: 1 / 由来: Homo sapiens
配列:

EAAGSCGPPP ELLNGNVKEK TKEEYGHSEV VEYYCNPRFL MKGPNKIQCV DGEWTTLPVC IVEESTCGDI PELEHGWAQL SSPPYYYGDS VEFNCSESFT MIGHRSITCI HGVWTQLPQC VAIDKLKKCK SSNLIILEEH LKNKKEFDHN SNIRYRCRGK EGWIHTVCIN GRWDPEVNCS MAQIQLCPPP PQIPNSHNMT TTLNYRDGEK VSVLCQENYL IQEGEEITCK DGRWQSIPLC VEK

実験情報

• ビーム: 設備名称: DORIS III X33 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron
• 検出器: 名称: MAR 345 Image Plate

スキャン

タイトル: fh1114 / 測定日: 2008年12月2日 / 保管温度: 10 °C / セル温度: 10 °C / 照射時間: 120 sec. / フレーム数: 1 / 単位: 1/nm /

	Min	Max
Q	0.2709	4.786

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.6 / ポイント数: 400 /

	Min	Max
Q	0.2732	3.029
P(R) point	1	400
R	0	10.5

結果

カーブのタイプ: merged / Standard: BSA /

	実験値	Standard	Porod
分子量	35 kDa	35 kDa	-
体積	-	-	38 nm3

/

	Guinier	P(R)
前方散乱 I0	0.38	-
慣性半径, Rg	3 nm	3.1 nm

/

	Min	Max
D	-	10.5
Guinier point	1	69