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データを開く
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基本情報
登録情報 | データベース: SASBDB / ID: SASDAT6 |
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![]() | Integrin beta4, fragment of the cytoplasmic region encompassing the third and fourth FnIII domains
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機能・相同性 | ![]() trophoblast cell migration / Type I hemidesmosome assembly / hemidesmosome assembly / nail development / hemidesmosome / peripheral nervous system myelin formation / Laminin interactions / skin morphogenesis / filopodium assembly / mesodermal cell differentiation ...trophoblast cell migration / Type I hemidesmosome assembly / hemidesmosome assembly / nail development / hemidesmosome / peripheral nervous system myelin formation / Laminin interactions / skin morphogenesis / filopodium assembly / mesodermal cell differentiation / Differentiation of keratinocytes in interfollicular epidermis in mammalian skin / integrin complex / Assembly of collagen fibrils and other multimeric structures / Syndecan interactions / cell leading edge / basement membrane / cell-matrix adhesion / basal plasma membrane / integrin-mediated signaling pathway / G protein-coupled receptor binding / cell motility / cell-cell adhesion / response to wounding / autophagy / integrin binding / cell junction / nuclear membrane / receptor complex / cell adhesion / focal adhesion / nucleolus / cell surface / extracellular exosome / metal ion binding / plasma membrane 類似検索 - 分子機能 |
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![]() | ![]() タイトル: Combination of X-ray crystallography, SAXS and DEER to obtain the structure of the FnIII-3,4 domains of integrin α6β4. 著者: Noelia Alonso-García / Inés García-Rubio / José A Manso / Rubén M Buey / Hector Urien / Arnoud Sonnenberg / Gunnar Jeschke / José M de Pereda / ![]() ![]() ![]() 要旨: Integrin α6β4 is a major component of hemidesmosomes that mediate the stable anchorage of epithelial cells to the underlying basement membrane. Integrin α6β4 has also been implicated in cell ...Integrin α6β4 is a major component of hemidesmosomes that mediate the stable anchorage of epithelial cells to the underlying basement membrane. Integrin α6β4 has also been implicated in cell proliferation and migration and in carcinoma progression. The third and fourth fibronectin type III domains (FnIII-3,4) of integrin β4 mediate binding to the hemidesmosomal proteins BPAG1e and BPAG2, and participate in signalling. Here, it is demonstrated that X-ray crystallography, small-angle X-ray scattering and double electron-electron resonance (DEER) complement each other to solve the structure of the FnIII-3,4 region. The crystal structures of the individual FnIII-3 and FnIII-4 domains were solved and the relative arrangement of the FnIII domains was elucidated by combining DEER with site-directed spin labelling. Multiple structures of the interdomain linker were modelled by Monte Carlo methods complying with DEER constraints, and the final structures were selected against experimental scattering data. FnIII-3,4 has a compact and cambered flat structure with an evolutionary conserved surface that is likely to correspond to a protein-interaction site. Finally, this hybrid method is of general application for the study of other macromolecules and complexes. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
-モデル
モデル #219 | ![]() タイプ: dummy / ソフトウェア: DAMMIF / ダミー原子の半径: 1.50 A / カイ2乗値: 1.976836 ![]() |
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モデル #220 | ![]() タイプ: atomic / ソフトウェア: CRYSOL / カイ2乗値: 1.297321 ![]() |
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試料
![]() | 名称: Integrin beta4, fragment of the cytoplasmic region encompassing the third and fourth FnIII domains 試料濃度: 1.50-48.60 |
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バッファ | 名称: Sodium Phosphate / 濃度: 20.00 mM / pH: 7.5 / 組成: 150 mM NaCl, 5% glycerol, 3 mM DTT |
要素 #133 | タイプ: protein / 記述: Integrin beta-4 / 分子量: 23.359 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: P16144 配列: GSHMVPDTPT RLVFSALGPT SLRVSWQEPR CERPLQGYSV EYQLLNGGEL HRLNIPNPAQ TSVVVEDLLP NHSYVFRVRA QSQEGWGRER EGVITIESQV HPQSPLCPLP GSAFTLSTPS APGPLVFTAL SPDSLQLSWE RPRRPNGDIV GYLVTCEMAQ GGGPATAFRV ...配列: GSHMVPDTPT RLVFSALGPT SLRVSWQEPR CERPLQGYSV EYQLLNGGEL HRLNIPNPAQ TSVVVEDLLP NHSYVFRVRA QSQEGWGRER EGVITIESQV HPQSPLCPLP GSAFTLSTPS APGPLVFTAL SPDSLQLSWE RPRRPNGDIV GYLVTCEMAQ GGGPATAFRV DGDSPESRLT VPGLSENVPY KFKVQARTTE GFGPEREGII TIES |
-実験情報
ビーム | 設備名称: PETRA III P12 / 地域: Hamburg / 国: Germany ![]() | |||||||||||||||||||||
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検出器 | 名称: Pilatus 2M | |||||||||||||||||||||
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距離分布関数 P(R) |
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結果 | コメント: Combined use of SAXS, crystal structures, site directed spin labeling, double electron-electron resonance (DEER), and molecular modeling to analyze the structure of FnIII-3,4.
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