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- SASDAN2: RNase in PBS (Ribonuclease pancreatic, RNase) -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDAN2
SampleRNase in PBS
  • Ribonuclease pancreatic (protein), RNase, Bos taurus
Function / homology
Function and homology information


pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / nucleic acid binding / lyase activity / defense response to Gram-positive bacterium / extracellular region
Similarity search - Function
Pancreatic ribonuclease / Ribonuclease A, active site / Ribonuclease A-domain / Ribonuclease A-like domain superfamily / Pancreatic ribonuclease / Pancreatic ribonuclease family signature. / Pancreatic ribonuclease
Similarity search - Domain/homology
Ribonuclease pancreatic
Similarity search - Component
Biological speciesBos taurus (cattle)
Contact author
  • Darja Ruskule (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #25
Type: dummy / Radius of dummy atoms: 1.90 A / Symmetry: P1
Comment: Gasbori run 10 times, damsel identified structure with lowest NSD
Chi-square value: 2.4025
Search similar-shape structures of this assembly by Omokage search (details)
Model #24
Type: atomic / Symmetry: P1 / Chi-square value: 1.493284
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: RNase in PBS / Ext coefficient: 6.9 / Contrast: 3.163 / Specific vol: 0.7279 / Purity method: gel filtration / Dry vol: 19917 / Sample MW: 16.46 kDa / Specimen concentration: 22.6 mg/ml / Concentration method: nanodrop
BufferName: PBS / PK: 7 / pH: 7.4
Entity #17Name: RNase / Type: protein / Description: Ribonuclease pancreatic / Formula weight: 16.46 / Num. of mol.: 1 / Source: Bos taurus / References: UniProt: P61823
Sequence:
MALKSLVLLS LLVLVLLLVR VQPSLGKETA AAKFERQHMD SSTSAASSSN YCNQMMKSRN LTKDRCKPVN TFVHESLADV QAVCSQKNVA CKNGQTNCYQ SYSTMSITDC RETGSSKYPN CAYKTTQANK HIIVACEGNP YVPVHFDASV

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Experimental information

BeamInstrument name: DORIS III EMBL X33 / City: Hamburg / 国: Germany / Shape: 0.6 / Type of source: X-ray synchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 2.7 mm
DetectorName: Pilatus 1M-W / Pixsize x: 0.172 mm
Scan
Title: RNase in PBS / Measurement date: Sep 18, 2012 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 15 sec. / Number of frames: 8 / Unit: 1/nm /
MinMax
Q0.0871 17.6809
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 801 /
MinMax
Q0.6312 2.82117
P(R) point1 801
R0 5
Result
Type of curve: merged / Standard: BSA / Comments: merged
ExperimentalStandardPorod
MW14.5 kDa14.5 kDa-
Volume--14.7 nm3

P(R)Guinier
Forward scattering, I013.4 13.5
Radius of gyration, Rg1.59 nm1.58 nm

MinMax
D-5
Guinier point136 271

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