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- SASDAN2: RNase in PBS (Ribonuclease pancreatic, RNase) -

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Basic information

Entry
Database: SASBDB / ID: SASDAN2
SampleRNase in PBSRibonuclease
  • Ribonuclease pancreaticPancreatic ribonuclease (protein), RNaseRibonuclease, Bos taurus
Function / homologyPancreatic ribonuclease / Ribonuclease A, active site / Ribonuclease A-domain / Ribonuclease A-like domain superfamily / Pancreatic ribonuclease / Pancreatic ribonuclease family signature. / pancreatic ribonuclease / ribonuclease A activity / ribonuclease activity / nucleic acid binding ...Pancreatic ribonuclease / Ribonuclease A, active site / Ribonuclease A-domain / Ribonuclease A-like domain superfamily / Pancreatic ribonuclease / Pancreatic ribonuclease family signature. / pancreatic ribonuclease / ribonuclease A activity / ribonuclease activity / nucleic acid binding / endonuclease activity / extracellular region / Ribonuclease pancreatic
Function and homology information
SourceBos taurus (cattle)
Contact authorDarja Ruskule (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #25
Type: dummy / Software: gasbor / Radius of dummy atoms: 1.90 A / Symmetry: P1
Comment: Gasbori run 10 times, damsel identified structure with lowest NSD
Chi-square value: 2.4025
Search similar-shape structures of this assembly by Omokage search (details)
Model #24
Type: atomic / Software: CRYSOL_30 / Symmetry: P1 / Chi-square value: 1.493284
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: RNase in PBSRibonuclease / Ext coefficient: 6.9 / Contrast: 3.163 / Specific vol: 0.7279 / Purity method: gel filtration / Dry vol: 19917 / Sample MW: 16.46 / Specimen concentration: 22.6 / Concentration method: nanodrop / Entity id: 17
BufferName: PBS / PK: 7 / pH: 7.4 / Sample id: 12
Entity #17Name: Pancreatic ribonuclease, RNaseRibonuclease, Ribonuclease
Type: protein / Description: Ribonuclease pancreaticPancreatic ribonuclease / Formula weight: 16.46 / Number of molecules: 1 / Source: Bos taurus / References: UniProt: P61823
Sequence:
MALKSLVLLS LLVLVLLLVR VQPSLGKETA AAKFERQHMD SSTSAASSSN YCNQMMKSRN LTKDRCKPVN TFVHESLADV QAVCSQKNVA CKNGQTNCYQ SYSTMSITDC RETGSSKYPN CAYKTTQANK HIIVACEGNP YVPVHFDASV

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Experimental information

BeamInstrument name: DORIS III X33 / Instrument city: Hamburg / Instrument country: Germany / Shape: 0.6 / Type of source: X-ray synchrotronSynchrotron / Radiation wavelength: 0.15 / Dist spec to detc: 2.7
DetectorName: Pilatus 1M-W / Pixsize x: 0.172
Scan
Title: WAXS / Detc id: 1 / Measurement date: Sep 18, 2012 / Storage temperature: 1 / Cell temperature: 1 / Exposure time: 15 / Number of frames: 8 / Unit: 1/nm / Qmin: 0.0871 / Qmax: 17.6809 / Sample id: 12 / Result id: 21 / Beam id: 1
Distance distribution function P(R)
Intensity id: 21 / Software p of R: GNOM 5.0 / Number of points: 801 / Qmin: 0.6312 / Qmax: 2.82117 / P of R point min: 1 / P of R point max: 801 / Rmin: 0 / Rmax: 5
Result
SASBDB code: SASDAN2 / Experimental MW: 14.5 / MW standard: 14.5 / I0 from PR: 13.4 / I0 from Guinier: 13.5 / Rg from PR: 1.59 / Rg from Guinier: 1.58 / D max: 5 / Porod volume: 14.7 / Guinier point min: 136 / Guinier point max: 271 / Type of curve: merged / Standard: BSA / Comments: merged

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