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- SASDAN2: RNase in PBS (Ribonuclease pancreatic, RNase) -

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Entry
Database: SASBDB / ID: SASDAN2
SampleRNase in PBS
Ribonuclease pancreatic (protein), RNase, Bos taurus
SourceBos taurus / mammal / ウシ /
Contact authorDarja Ruskule (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Models

Model #25
Type: dummy / Software: gasbor / Radius of dummy atoms: 1.90 A / Symmetry: P1
Comment: Gasbori run 10 times, damsel identified structure with lowest NSD
Chi-square value: 2.4025
Search similar-shape structures of this assembly by Omokage search (details)
Model #24
Type: atomic / Software: CRYSOL_30 / Symmetry: P1 / Chi-square value: 1.493284
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: RNase in PBS / Ext coefficient: 6.9 / Contrast: 3.163 / Specific vol: 0.7279 / Purity method: gel filtration / Dry vol: 19917 / Sample MW: 16.46 / Specimen concentration: 22.6 / Concentration method: nanodrop
BufferName: PBS / PK: 7 / pH: 7.4
Entity #17Type: protein / Description: Ribonuclease pancreatic / Formula weight: 16.46 / Number of molecules: 1 / Source: Bos taurus / References: UniProt: P61823
Sequence:
MALKSLVLLS LLVLVLLLVR VQPSLGKETA AAKFERQHMD SSTSAASSSN YCNQMMKSRN LTKDRCKPVN TFVHESLADV QAVCSQKNVA CKNGQTNCYQ SYSTMSITDC RETGSSKYPN CAYKTTQANK HIIVACEGNP YVPVHFDASV

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Experimental information

BeamInstrument name: DORIS III X33 / Instrument city: Hamburg / Instrument country: Germany / Shape: 0.6 / Type of source: X-ray synchrotron / Radiation wavelength: 0.15 / Dist spec to detc: 2.7
ScanTitle: WAXS / Measurement date: Sep 18, 2012 / Storage temperature: 1 / Cell temperature: 1 / Exposure time: 15 / Number of frames: 8 / Unit: 1/nm / Qmin: 0.0871 / Qmax: 17.6809
Distance distribution function P(R)Software p of R: GNOM 5.0 / Number of points: 801 / Qmin: 0.6312 / Qmax: 2.82117 / P of R point min: 1 / P of R point max: 801 / Rmin: 0 / Rmax: 5
ResultSASBDB code: SASDAN2 / Experimental MW: 14.5 / MW standard: 14.5 / I0 from PR: 13.4 / I0 from Guinier: 13.5 / Rg from PR: 1.59 / Rg from Guinier: 1.58 / D max: 5 / Porod volume: 14.7 / Guinier point min: 136 / Guinier point max: 271 / Type of curve: merged / Standard: BSA / Comments: merged

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