Name: Tris / Concentration: 50.00 mM / pH: 8 Comment: TCEP does not reduce metals in solution, but prevents aggregation and precipitation of the protein Composition: 150 mM NaCl
2 mM TCEP
Entity #157
Name: hETHE1 / Type: protein / Description: Persulfide dioxygenase ETHE1, mitochondrial / Formula weight: 27.873 / Num. of mol.: 2 / Source: Homo sapiens / References: UniProt: O95571
-
Experimental information
Beam
Instrument name: DORIS III X33 / City: Hamburg / 国: Germany / Shape: 0.6 / Type of source: X-ray synchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 2.7 mm
Detector
Name: Pilatus 1M-W / Pixsize x: 0.172 mm
Scan
Title: Metal-bound hETHE1 / Measurement date: Oct 21, 2007 / Cell temperature: 9 °C / Exposure time: 30 sec. / Number of frames: 4 / Unit: 1/nm /
Min
Max
Q
0.0835
4.981
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1369 /
Min
Max
Q
0.155228
3.3215
P(R) point
1
1369
R
0
7.5
Result
Type of curve: merged Comments: Metal-bound hETHE1 was shown by SAXS to be a globular protein compatible with a dimeric hETHE1 in solution. The calculated scattering pattern from a high resolution model of dimeric ETHE1 ...Comments: Metal-bound hETHE1 was shown by SAXS to be a globular protein compatible with a dimeric hETHE1 in solution. The calculated scattering pattern from a high resolution model of dimeric ETHE1 is in a very good agreement (χ2 value of 1.5) with the experimental data from hETHE1. The low resolution shape of metal-bound hETHE1 was reconstructed ab initio using DAMMIN with P2 symmetry imposed. The ab initio model fits the experimental data with a discrepancy χ2 = 1.3 and is in a good agreement with the high resolution ETHE1 structure.
Experimental
Experimental error
Porod
Porod error
Estimated
Estimated method
MW
42 kDa
4
42 kDa
4
-
-
Volume
-
-
70 nm3
7
78.87
DAMMIN
P(R)
P(R) error
Guinier
Guinier error
Forward scattering, I0
98.52
0.1952
99.96
0.29
Radius of gyration, Rg
2.408 nm
0.004
2.47 nm
0.24
Min
Max
Error
D
-
7.5
1
Guinier point
32
293
-
+
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