SASDAA5: CHD4 (AH) (Human Chromatin Remodeler CHD4 (685-1233))

基本情報

• 登録情報: データベース: SASBDB / ID: SASDAA5

試料

CHD4 (AH)

• Human Chromatin Remodeler CHD4 (685-1233) (protein), CHD4 (AH), Homo sapiens

• 生物種: Homo sapiens (ヒト)
• 引用: ジャーナル: J Mol Biol / 年: 2012; タイトル: The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.; 著者: Aleksandra A Watson / Pravin Mahajan / Haydyn D T Mertens / Michael J Deery / Wenchao Zhang / Peter Pham / Xiuxia Du / Till Bartke / Wei Zhang / Christian Edlich / Georgina Berridge / Yun Chen / Nicola A Burgess-Brown / Tony Kouzarides / Nicola Wiechens / Tom Owen-Hughes / Dmitri I Svergun / Opher Gileadi / Ernest D Laue / ; 要旨: The NuRD (nucleosome remodeling and deacetylase) complex serves as a crucial epigenetic regulator of cell differentiation, proliferation, and hematopoietic development by coupling the deacetylation and demethylation of histones, nucleosome mobilization, and the recruitment of transcription factors. The core nucleosome remodeling function of the mammalian NuRD complex is executed by the helicase-domain-containing ATPase CHD4 (Mi-2β) subunit, which also contains N-terminal plant homeodomain (PHD) and chromo domains. The mode of regulation of chromatin remodeling by CHD4 is not well understood, nor is the role of its PHD and chromo domains. Here, we use small-angle X-ray scattering, nucleosome binding ATPase and remodeling assays, limited proteolysis, cross-linking, and tandem mass spectrometry to propose a three-dimensional structural model describing the overall shape and domain interactions of CHD4 and discuss the relevance of these for regulating the remodeling of chromatin by the NuRD complex.

登録者

• Haydyn Mertens (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

モデル

• モデル #100: ; タイプ: dummy / ソフトウェア: dammif / ダミー原子の半径: 1.90 A / カイ2乗値: 1.592644; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: CHD4 (AH) / Sample MW: 62.8 kDa / 試料濃度: 1.40-8.60
• バッファ: 名称: HEPES / 濃度: 50.00 mM / PK: 7 / pH: 7.5 ; コメント: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; 組成: KCl 50.000 mM

要素 #84

名称: CHD4 (AH) / タイプ: protein / 記述: Human Chromatin Remodeler CHD4 (685-1233) / 分子量: 62.8 / 分子数: 1 / 由来: Homo sapiens
配列:

MPGKKLKKVK LRKLERPPET PTVDPTVKYE RQPEYLDATG GTLHPYQMEG LNWLRFSWAQ GTDTILADEM GLGKTVQTAV FLYSLYKEGH SKGPFLVSAP LSTIINWERE FEMWAPDMYV VTYVGDKDSR AIIRENEFSF EDNAIRGGKK ASRMKKEASV KFHVLLTSYE LITIDMAILG SIDWACLIVD EAHRLKNNQS KFFRVLNGYS LQHKLLLTGT PLQNNLEELF HLLNFLTPER FHNLEGFLEE FADIAKEDQI KKLHDMLGPH MLRRLKADVF KNMPSKTELI VRVELSPMQK KYYKYILTRN FEALNARGGG NQVSLLNVVM DLKKCCNHPY LFPVAAMEAP KMPNGMYDGS ALIRASGKLL LLQKMLKNLK EGGHRVLIFS QMTKMLDLLE DFLEHEGYKY ERIDGGITGN MRQEAIDRFN APGAQQFCFL LSTRAGGLGI NLATADTVII YDSDWNPHND IQAFSRAHRI GQNKKVMIYR FVTRASVEER ITQVAKKKMM LTHLVVRPGL GSKTGSMSKQ ELDDILKFGT EELFKDEATD

実験情報

• ビーム: 設備名称: DORIS III X33 / 地域: Hamburg / 国: Germany / 形状: 0.6 / 線源: X-ray synchrotron / 波長: 0.15 Å / スペクトロメータ・検出器間距離: 2.7 mm
• 検出器: 名称: Pilatus 1M-W / Pixsize x: 0.172 mm

スキャン

タイトル: CHD4 (AH) / 測定日: 2010年11月13日 / 保管温度: 10 °C / セル温度: 10 °C / 照射時間: 15 sec. / フレーム数: 8 / 単位: 1/nm /

	Min	Max
Q	0.1985	6.0155

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.5a / ポイント数: 302 /

	Min	Max
Q	0.1998	1.847
P(R) point	52	353
R	0	13.22

結果

カーブのタイプ: extrapolated / Standard: BSA
コメント: The ATPase CHD4 mediates nucleosome remodeling by the NuRD (nucleosome remodeling and deacetylase) complex. The NuRD complex serves as a crucial epigenetic regulator of cell differentiation, proliferation, and hematopoietic development by coupling the deacetylation and demethylation of histones, nucleosome mobilization, and the recruitment of transcription factors. The three dimensional small-angle X-ray scattering model of CHD4 helps to define its interdomain interactions, with cross linking and limited proteolysis studies used to validate the model. Functional and binding assays suggest a regulatory role for the PHD and chromo domains.

	実験値	Standard
分子量	52 kDa	52 kDa

	Guinier	P(R)
前方散乱 I0	45.63	-
慣性半径, Rg	4 nm	4.2 nm

	Min	Max
D	-	14.5
Guinier point	1	52