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データを開く
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基本情報
| 登録情報 | データベース: PDB / ID: 9n73 | |||||||||||||||
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| タイトル | SSU processome maturation and disassembly, State G | |||||||||||||||
要素 |
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キーワード | RIBOSOME / SSU processome / ribosome assembly / RNA folding / RNA-protein interactions | |||||||||||||||
| 機能・相同性 | 機能・相同性情報18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / nuclear polyadenylation-dependent antisense transcript catabolic process / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / nuclear polyadenylation-dependent mRNA catabolic process / tRNA wobble cytosine modification / regulation of ribosomal protein gene transcription by RNA polymerase II / tRNA cytidine N4-acetyltransferase activity / rRNA acetylation involved in maturation of SSU-rRNA ...18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / nuclear polyadenylation-dependent antisense transcript catabolic process / nuclear polyadenylation-dependent snoRNA catabolic process / nuclear polyadenylation-dependent snRNA catabolic process / nuclear polyadenylation-dependent mRNA catabolic process / tRNA wobble cytosine modification / regulation of ribosomal protein gene transcription by RNA polymerase II / tRNA cytidine N4-acetyltransferase activity / rRNA acetylation involved in maturation of SSU-rRNA / 18S rRNA cytidine N-acetyltransferase activity / U1 snRNA 3'-end processing / tRNA acetylation / rRNA small subunit pseudouridine methyltransferase Nep1 / box H/ACA snoRNA binding / nuclear polyadenylation-dependent CUT catabolic process / RNA fragment catabolic process / U5 snRNA 3'-end processing / Noc4p-Nop14p complex / rRNA 2'-O-methylation / TRAMP-dependent tRNA surveillance pathway / exosome (RNase complex) / CURI complex / UTP-C complex / U4 snRNA 3'-end processing / endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / t-UTP complex / nuclear polyadenylation-dependent rRNA catabolic process / poly(A)-dependent snoRNA 3'-end processing / nuclear microtubule / snoRNA guided rRNA 2'-O-methylation / Pwp2p-containing subcomplex of 90S preribosome / Mpp10 complex / nuclear exosome (RNase complex) / rRNA (pseudouridine) methyltransferase activity / rRNA modification / exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / histone H2AQ104 methyltransferase activity / box C/D sno(s)RNA binding / regulation of rRNA processing / septum digestion after cytokinesis / tRNA re-export from nucleus / snRNA binding / rRNA (adenine-N6,N6-)-dimethyltransferase activity / histone mRNA catabolic process / RNA folding chaperone / box C/D sno(s)RNA 3'-end processing / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA methyltransferase activity / rDNA heterochromatin / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear mRNA surveillance / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / regulation of transcription by RNA polymerase I / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / box C/D methylation guide snoRNP complex / U4/U6 snRNP / positive regulation of rRNA processing / tRNA export from nucleus / single-stranded telomeric DNA binding / rRNA primary transcript binding / sno(s)RNA-containing ribonucleoprotein complex / Negative regulators of DDX58/IFIH1 signaling / rRNA base methylation / U4 snRNA binding / O-methyltransferase activity / small nuclear ribonucleoprotein complex / SUMOylation of RNA binding proteins / protein localization to nucleolus / 90S preribosome assembly / mTORC1-mediated signalling / rRNA methylation / Protein hydroxylation / RNA catabolic process / mRNA modification / U4 snRNP / regulation of telomere maintenance / poly(U) RNA binding / U3 snoRNA binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / poly(A)+ mRNA export from nucleus / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / snoRNA binding / preribosome, small subunit precursor / precatalytic spliceosome / Major pathway of rRNA processing in the nucleolus and cytosol / establishment of cell polarity / spliceosomal complex assembly / positive regulation of transcription by RNA polymerase I / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / nucleolar large rRNA transcription by RNA polymerase I / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / 90S preribosome 類似検索 - 分子機能 | |||||||||||||||
| 生物種 | ![]() | |||||||||||||||
| 手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 5.96 Å | |||||||||||||||
データ登録者 | Buzovetsky, O. / Klinge, S. | |||||||||||||||
| 資金援助 | 米国, 2件
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引用 | ジャーナル: To Be Publishedタイトル: Mechanism of helicase-mediated SSU processome maturation and disassembly 著者: Buzovetsky, O. / Klinge, S. | |||||||||||||||
| 履歴 |
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構造の表示
| 構造ビューア | 分子: Molmil Jmol/JSmol |
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ダウンロードとリンク
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ダウンロード
| PDBx/mmCIF形式 | 9n73.cif.gz | 4.5 MB | 表示 | PDBx/mmCIF形式 |
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| PDB形式 | pdb9n73.ent.gz | 表示 | PDB形式 | |
| PDBx/mmJSON形式 | 9n73.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
| その他 | その他のダウンロード |
-検証レポート
| 文書・要旨 | 9n73_validation.pdf.gz | 1.9 MB | 表示 | wwPDB検証レポート |
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| 文書・詳細版 | 9n73_full_validation.pdf.gz | 1.9 MB | 表示 | |
| XML形式データ | 9n73_validation.xml.gz | 487.2 KB | 表示 | |
| CIF形式データ | 9n73_validation.cif.gz | 880.7 KB | 表示 | |
| アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/n7/9n73 ftp://data.pdbj.org/pub/pdb/validation_reports/n7/9n73 | HTTPS FTP |
-関連構造データ
| 関連構造データ | ![]() 49083MC ![]() 9n74C ![]() 9n7bC ![]() 48985 ![]() 48986 ![]() 48987 ![]() 48988 ![]() 48989 ![]() 48990 ![]() 48991 ![]() 48992 M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
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集合体
| 登録構造単位 | ![]()
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| 1 |
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要素
+RNA鎖 , 3種, 3分子 L0L1L2
+40S ribosomal protein ... , 18種, 18分子 L3L4L5L6L7L8L9LCLDLELFLGNFNGNPNQOHSR
+タンパク質 , 19種, 23分子 LHLOLULXLYNBNDNLNMNNNSNVOAOUSCSDSESFSHSJSKSWSZ
+U3 small nucleolar RNA-associated protein ... , 17種, 17分子 LILJLKLLLMLNLPLQLRLSLTLWNANHSPSSSY
+Ribosome biogenesis protein ... , 2種, 2分子 LVSI
+U3 small nucleolar ribonucleoprotein protein ... , 3種, 3分子 LZNCSM
+Ribosomal RNA-processing protein ... , 2種, 2分子 NISG
+Nucleolar protein ... , 2種, 2分子 SASB
+RRNA-processing protein ... , 2種, 2分子 SLSQ
+Nucleolar complex protein ... , 2種, 2分子 STSU
+詳細
-実験情報
-実験
| 実験 | 手法: 電子顕微鏡法 |
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| EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
| 構成要素 | 名称: SSU processome maturation and disassembly, State G / タイプ: COMPLEX / Entity ID: all / 由来: NATURAL | ||||||||||||||||||||||||||||
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| 由来(天然) | 生物種: ![]() | ||||||||||||||||||||||||||||
| 緩衝液 | pH: 7.5 | ||||||||||||||||||||||||||||
| 緩衝液成分 |
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| 試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES | ||||||||||||||||||||||||||||
| 急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
| 実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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| 顕微鏡 | モデル: TFS KRIOS |
| 電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: OTHER |
| 電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 25000 nm / 最小 デフォーカス(公称値): 1000 nm |
| 撮影 | 電子線照射量: 60 e/Å2 / フィルム・検出器のモデル: GATAN K3 (6k x 4k) |
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解析
| CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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| 3次元再構成 | 解像度: 5.96 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 4987 / 対称性のタイプ: POINT |
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万見について






米国, 2件
引用





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FIELD EMISSION GUN