- PDB-8j9i: Cryo-EM structure of Euglena gracilis complex I, turnover state -
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基本情報
登録情報
データベース: PDB / ID: 8j9i
タイトル
Cryo-EM structure of Euglena gracilis complex I, turnover state
要素
NADH-ubiquinone oxidoreductase chain 4
ND1
ND2A
ND2B
ND3
ND4L
ND5
NDUCA1
NDUCA2
NDUCA3
NDUEG1
NDUEG10
NDUEG11
NDUEG12
NDUEG13
NDUEG2
NDUEG3
NDUEG4
NDUEG5
NDUEG6
NDUEG7
NDUEG8
NDUFA1
NDUFA11
NDUFA12
NDUFA13
NDUFA2
NDUFA3
NDUFA5
NDUFA6
NDUFA7
NDUFA8
NDUFA9
NDUFAB1-alpha
NDUFAB1-beta
NDUFB10
NDUFB11
NDUFB2
NDUFB3
NDUFB4
NDUFB5
NDUFB6
NDUFB7
NDUFB8
NDUFB9
NDUFC2
NDUFS1A
NDUFS1B
NDUFS2
NDUFS3
NDUFS4
NDUFS5
NDUFS6
NDUFS7
NDUFS8
NDUFV1
NDUFV2
NDUFX
UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK-UNK
キーワード
ELECTRON TRANSPORT / Electron transport chain / respiratory complex / membrane protein / Euglena gracilis
機能・相同性
機能・相同性情報
respiratory chain complex I / NADH:ubiquinone reductase (H+-translocating) / ubiquinone binding / NADH dehydrogenase (ubiquinone) activity / electron transport coupled proton transport / ATP synthesis coupled electron transport / mitochondrial membrane 類似検索 - 分子機能
ジャーナル: Nat Commun / 年: 2024 タイトル: Euglena's atypical respiratory chain adapts to the discoidal cristae and flexible metabolism. 著者: Zhaoxiang He / Mengchen Wu / Hongtao Tian / Liangdong Wang / Yiqi Hu / Fangzhu Han / Jiancang Zhou / Yong Wang / Long Zhou / 要旨: Euglena gracilis, a model organism of the eukaryotic supergroup Discoba harbouring also clinically important parasitic species, possesses diverse metabolic strategies and an atypical electron ...Euglena gracilis, a model organism of the eukaryotic supergroup Discoba harbouring also clinically important parasitic species, possesses diverse metabolic strategies and an atypical electron transport chain. While structures of the electron transport chain complexes and supercomplexes of most other eukaryotic clades have been reported, no similar structure is currently available for Discoba, limiting the understandings of its core metabolism and leaving a gap in the evolutionary tree of eukaryotic bioenergetics. Here, we report high-resolution cryo-EM structures of Euglena's respirasome I + III + IV and supercomplex III + IV. A previously unreported fatty acid synthesis domain locates on the tip of complex I's peripheral arm, providing a clear picture of its atypical subunit composition identified previously. Individual complexes are re-arranged in the respirasome to adapt to the non-uniform membrane curvature of the discoidal cristae. Furthermore, Euglena's conformationally rigid complex I is deactivated by restricting ubiquinone's access to its substrate tunnel. Our findings provide structural insights for therapeutic developments against euglenozoan parasite infections.