[English] 日本語
Yorodumi
- PDB-8gym: Cryo-EM structure of Tetrahymena thermophila respiratory mega-com... -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: PDB / ID: 8gym
TitleCryo-EM structure of Tetrahymena thermophila respiratory mega-complex MC IV2+(I+III2+II)2
Components
  • (Acyl carrier ...) x 2
  • (Cytochrome c oxidase subunit ...) x 3
  • (Gamma-carbonic ...) x 2
  • (NADH dehydrogenase [ubiquinone] ...) x 8
  • (NADH dehydrogenase subunit ...) x 6
  • (NADH-ubiquinone oxidoreductase ...) x 7
  • (SURF1-like protein) x 2
  • (Succinate dehydrogenase ...) x 2
  • (Transmembrane ...) x 33
  • (Transposase) x 2
  • (Ubiquinol-cytochrome ...) x 2
  • (Unknown peptide) x 2
  • (Zf-Tim10_DDP domain-containing ...) x 2
  • 2 iron, 2 sulfur cluster-binding protein
  • 2-oxoglutarate/malate carrier protein
  • 37S ribosomal protein S25, mitochondrial
  • 39S ribosomal protein L9, mitochondrial
  • ABC transporter
  • ATP synthase subunit e, mitochondrial
  • Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II
  • Annexin
  • Apocytochrome b
  • CHCH domain-containing protein
  • COX assembly mitochondrial protein
  • COXTT10
  • COXTT22
  • COXTT28
  • COXTT3
  • COXTT9
  • CTF/NF-I domain-containing protein
  • CX9C domain-containing protein
  • Carrier protein
  • Chromosome condensation regulator RCC1 repeat protein
  • Complex I-MNLL
  • Complex III subunit VII
  • Cullin domain-containing protein
  • Cytochrome b-c1 complex subunit 8
  • Cytochrome protein c1
  • DUF4885 domain-containing protein
  • Decapping nuclease
  • Diphthamide synthesis protein
  • DnaJ domain protein
  • ETC complex I subunit motif protein
  • GRAM domain protein
  • Iron-binding zinc finger CDGSH type protein
  • Lipid-A-disaccharide synthase
  • Lysozyme
  • M16 family peptidase, putative
  • Mobilization protein
  • NAD-dependent epimerase/dehydratase family protein
  • NADH dehydrogenase, putative
  • NDUA13
  • NDUB10
  • NDUB2
  • NDUB4
  • NDUB6
  • NDUB8
  • NDUPH2
  • NDUTT15
  • NDUTT16
  • NDUTT17
  • NmrA domain-containing protein
  • Oxoglutarate/malate translocator protein, putative
  • PH domain-containing protein
  • Peptidase M16 inactive domain protein
  • Phage protein
  • Protein phosphatase 2C, putative
  • RNase III domain-containing protein
  • Ribosomal protein L51/S25/CI-B8 domain protein
  • Rieske iron-sulfur protein, ubiquinol-cytochrome C reductase iron-sulfur subunit
  • SDHD
  • SDHTT11
  • SDHTT3
  • Structural protein
  • Sulphotransf domain-containing protein
  • Thioredoxin
  • Tim10/DDP family zinc finger protein
  • TraB family protein
  • Transcription factor apfi protein, putative
  • UQCRTT2
  • YflT domain-containing protein
  • Ymf57
  • Ymf58
  • Ymf62
  • Ymf65
  • Ymf67
  • Ymf68
  • Ymf70
  • Ymf75
  • Zinc-finger protein
KeywordsELECTRON TRANSPORT / Electron transport chain / supercomplex / membrane protein / Tetrahymena thermophila
Function / homology
Function and homology information


NADH:ubiquinone reductase (H+-translocating) / thiosulfate transmembrane transporter activity / oxaloacetate transmembrane transporter activity / lipid-A-disaccharide synthase / lipid-A-disaccharide synthase activity / malate transmembrane transporter activity / quinol-cytochrome-c reductase / protein maturation by [2Fe-2S] cluster transfer / medium-chain fatty acid-CoA ligase activity / succinate transmembrane transporter activity ...NADH:ubiquinone reductase (H+-translocating) / thiosulfate transmembrane transporter activity / oxaloacetate transmembrane transporter activity / lipid-A-disaccharide synthase / lipid-A-disaccharide synthase activity / malate transmembrane transporter activity / quinol-cytochrome-c reductase / protein maturation by [2Fe-2S] cluster transfer / medium-chain fatty acid-CoA ligase activity / succinate transmembrane transporter activity / P450-containing electron transport chain / : / sulfate transmembrane transporter activity / mitochondrial electron transport, succinate to ubiquinone / mitochondrial cytochrome c oxidase assembly / protein processing involved in protein targeting to mitochondrion / succinate dehydrogenase / succinate dehydrogenase (quinone) activity / phosphate ion transmembrane transport / mitochondrial respiratory chain complex IV / mitochondrial respiratory chain complex III / cytochrome-c oxidase / antiporter activity / ubiquinol-cytochrome-c reductase activity / ubiquinone-6 biosynthetic process / oxidoreductase activity, acting on NAD(P)H / mitochondrial electron transport, cytochrome c to oxygen / anaerobic respiration / cytochrome-c oxidase activity / 3 iron, 4 sulfur cluster binding / lipid A biosynthetic process / mitochondrial electron transport, ubiquinol to cytochrome c / NADH:ubiquinone reductase (H+-translocating) / Golgi organization / NADH dehydrogenase activity / : / mitochondrial electron transport, NADH to ubiquinone / respirasome / transmembrane transporter activity / endoplasmic reticulum-Golgi intermediate compartment / chloroplast thylakoid membrane / ubiquinone binding / mitochondrial respiratory chain complex I assembly / acyl binding / NADH dehydrogenase (ubiquinone) activity / acyl carrier activity / quinone binding / electron transport coupled proton transport / ATP synthesis coupled electron transport / tricarboxylic acid cycle / aerobic respiration / respiratory electron transport chain / mitochondrion organization / fatty acid metabolic process / chloroplast / mitochondrial membrane / electron transport chain / phospholipid binding / metalloendopeptidase activity / 2 iron, 2 sulfur cluster binding / NAD binding / protein transport / FMN binding / flavin adenine dinucleotide binding / 4 iron, 4 sulfur cluster binding / response to oxidative stress / mitochondrial inner membrane / membrane => GO:0016020 / oxidoreductase activity / electron transfer activity / ribosome / copper ion binding / DNA-binding transcription factor activity / heme binding / protein-containing complex binding / negative regulation of apoptotic process / protein kinase binding / endoplasmic reticulum / mitochondrion / membrane / nucleus / metal ion binding / plasma membrane / cytosol
Similarity search - Function
Surfeit locus 1/Shy1 / Surfeit locus 1/4 / TraB/PryY-like / SURF1 family / SURF1 family profile. / CTF transcription factor/nuclear factor 1, DNA-binding domain / CTF/NF-I DNA-binding domain profile. / Glycosyl transferase, family 19 / Lipid-A-disaccharide synthetase / Tim10-like ...Surfeit locus 1/Shy1 / Surfeit locus 1/4 / TraB/PryY-like / SURF1 family / SURF1 family profile. / CTF transcription factor/nuclear factor 1, DNA-binding domain / CTF/NF-I DNA-binding domain profile. / Glycosyl transferase, family 19 / Lipid-A-disaccharide synthetase / Tim10-like / Tim10-like domain superfamily / Tim10/DDP family zinc finger / Mitochondrial substrate/solute carrier / Mitochondrial carrier domain superfamily / : / Mitochondrial carrier protein / Solute carrier (Solcar) repeat profile. / Iron-binding zinc finger, CDGSH type / MitoNEET, CDGSH iron-sulfur domain / CDGSH-type zinc finger. Function unknown. / Dynactin subunit 5 / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / Succinate dehydrogenase, flavoprotein subunit / Adrenodoxin / Succinate dehydrogenase/fumarate reductase, flavoprotein subunit / Fumarate reductase/succinate dehydrogenase, FAD-binding site / FAD-dependent oxidoreductase SdhA/FrdA/AprA / Fumarate reductase / succinate dehydrogenase FAD-binding site. / 4Fe-4S dicluster domain / Succinate dehydrogenase/fumarate reductase iron-sulphur protein / Succinate dehydogenase/fumarate reductase N-terminal / 2Fe-2S iron-sulfur cluster binding domain / Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal / Fumarate reductase flavoprotein C-term / Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain superfamily / Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain superfamily / FAD-dependent oxidoreductase 2, FAD binding domain / Alpha-helical ferredoxin / Cytochrome c oxidase subunit Vb, zinc binding region signature. / FAD binding domain / Cytochrome c oxidase, subunit Vb / Cytochrome c oxidase, subunit Vb superfamily / Cytochrome c oxidase subunit Vb / Cytochrome c oxidase subunit Vb, zinc binding domain profile. / DnaJ domain / Ubiquinol-cytochrome C reductase hinge domain / Ubiquinol-cytochrome C reductase hinge domain superfamily / Ubiquinol-cytochrome C reductase hinge protein / Cytochrome c/quinol oxidase subunit II / DnaJ molecular chaperone homology domain / dnaJ domain profile. / Cytochrome c1 / Cytochrome C1 family / 2Fe-2S ferredoxin, iron-sulphur binding site / 2Fe-2S ferredoxin-type iron-sulfur binding region signature. / Chaperone J-domain superfamily / Cytochrome b/b6, C-terminal domain superfamily / Cytochrome b/b6/petB / DnaJ domain / Cytochrome c oxidase, subunit I, copper-binding site / Heme-copper oxidase catalytic subunit, copper B binding region signature. / Cytochrome c oxidase-like, subunit I domain / Cytochrome oxidase subunit I profile. / Cytochrome c oxidase subunit I / Cytochrome c oxidase-like, subunit I superfamily / Cytochrome C and Quinol oxidase polypeptide I / Hexapeptide repeat / Rieske iron-sulphur protein, C-terminal / Cytochrome b/b6, N-terminal / Cytochrome b/b6-like domain superfamily / Cytochrome b/b6 N-terminal region profile. / Di-haem cytochrome, transmembrane / Cytochrome C oxidase subunit II, periplasmic domain / Soluble ligand binding domain / 2Fe-2S iron-sulfur cluster binding domain / Cytochrome c oxidase subunit II-like C-terminal / SLBB domain / Cytochrome oxidase subunit II copper A binding domain profile. / Rieske iron-sulphur protein / Bacterial transferase hexapeptide (six repeats) / Zinc finger, CHCC-type / Zinc-finger domain / Peptidase M16, C-terminal / Peptidase M16 inactive domain / Peptidase M16, N-terminal / Insulinase (Peptidase family M16) / Metalloenzyme, LuxS/M16 peptidase-like / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / ETC complex I subunit conserved region / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / Thioredoxin / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal
Similarity search - Domain/homology
1,2-Distearoyl-sn-glycerophosphoethanolamine / ADENOSINE-5'-DIPHOSPHATE / CARDIOLIPIN / COPPER (II) ION / FE3-S4 CLUSTER / FLAVIN-ADENINE DINUCLEOTIDE / FE2/S2 (INORGANIC) CLUSTER / FLAVIN MONONUCLEOTIDE / HEME-A / HEME C ...1,2-Distearoyl-sn-glycerophosphoethanolamine / ADENOSINE-5'-DIPHOSPHATE / CARDIOLIPIN / COPPER (II) ION / FE3-S4 CLUSTER / FLAVIN-ADENINE DINUCLEOTIDE / FE2/S2 (INORGANIC) CLUSTER / FLAVIN MONONUCLEOTIDE / HEME-A / HEME C / PROTOPORPHYRIN IX CONTAINING FE / Chem-NDP / Chem-P5S / 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE / IRON/SULFUR CLUSTER / Phosphatidylinositol / UBIQUINONE-10 / Chem-ZMP / Transmembrane protein, putative / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / Oxoglutarate/malate translocator protein, putative / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / Transmembrane protein, putative / Transmembrane protein / SURF1-like protein / Uncharacterized protein / Uncharacterized protein / Diphthamide synthesis protein / Transmembrane protein / Transmembrane protein, putative / Uncharacterized protein / SURF1-like protein / Transmembrane protein / NADH dehydrogenase, putative / Transmembrane protein / succinate dehydrogenase / Transmembrane protein, putative / Gamma-carbonic anhydrase / Transmembrane protein / Iron-binding zinc finger CDGSH type protein / Transcription factor apfi protein, putative / Uncharacterized protein / Transmembrane protein, putative / Uncharacterized protein / Ribosomal protein L51/S25/CI-B8 domain protein / Transmembrane protein / Acyl carrier protein / Transmembrane protein / NADH-ubiquinone oxidoreductase 1, chain, putative / NADH-ubiquinone oxidoreductase 24 kDa subunit / Uncharacterized protein / Transmembrane protein, putative / Transmembrane protein / Transmembrane protein / Transmembrane protein, putative / Peptidase M16 inactive domain protein / Transmembrane protein, putative / Rieske iron-sulfur protein, ubiquinol-cytochrome C reductase iron-sulfur subunit / Transmembrane protein / Uncharacterized protein / Transmembrane protein, putative / Uncharacterized protein / M16 family peptidase, putative / Zinc-finger protein / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / Transmembrane protein / NAD-dependent epimerase/dehydratase family protein / Transmembrane protein, putative / Transmembrane protein / NADH dehydrogenase subunit 1 / Uncharacterized protein / Thioredoxin / Ubiquinol-cytochrome C reductase hinge protein / CTF/NF-I domain-containing protein / Transmembrane protein, putative / Transmembrane protein, putative / DnaJ domain protein / lipid-A-disaccharide synthase / Transmembrane protein / Protein phosphatase 2C, putative / Transmembrane protein, putative / Transmembrane protein, putative / Uncharacterized protein / Transmembrane protein, putative / Transmembrane protein / Transmembrane protein, putative / 2 iron, 2 sulfur cluster-binding protein / Transmembrane protein, putative / Transmembrane protein / Acyl carrier protein / Gamma-carbonic anhydrase / Uncharacterized protein / Transmembrane protein, putative / Carrier protein / Uncharacterized protein / Tim10/DDP family zinc finger protein / Transmembrane protein / Uncharacterized protein / Uncharacterized protein
Similarity search - Component
Biological speciesTetrahymena thermophila SB210 (eukaryote)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.96 Å
AuthorsWu, M.C. / Hu, Y.Q. / Han, F.Z. / Zhou, L.
Funding support China, 1items
OrganizationGrant numberCountry
Other governmentZJU100 Young Professor China
CitationJournal: Nat Commun / Year: 2023
Title: Structures of Tetrahymena thermophila respiratory megacomplexes on the tubular mitochondrial cristae.
Authors: Fangzhu Han / Yiqi Hu / Mengchen Wu / Zhaoxiang He / Hongtao Tian / Long Zhou /
Abstract: Tetrahymena thermophila, a classic ciliate model organism, has been shown to possess tubular mitochondrial cristae and highly divergent electron transport chain involving four transmembrane protein ...Tetrahymena thermophila, a classic ciliate model organism, has been shown to possess tubular mitochondrial cristae and highly divergent electron transport chain involving four transmembrane protein complexes (I-IV). Here we report cryo-EM structures of its ~8 MDa megacomplex IV+ (I + III+ II), as well as a ~ 10.6 MDa megacomplex (IV + I + III+ II) at lower resolution. In megacomplex IV+ (I + III+ II), each CIV protomer associates one copy of supercomplex I + III and one copy of CII, forming a half ring-shaped architecture that adapts to the membrane curvature of mitochondrial cristae. Megacomplex (IV+ I + III+ II) defines the relative position between neighbouring half rings and maintains the proximity between CIV and CIII cytochrome c binding sites. Our findings expand the current understanding of divergence in eukaryotic electron transport chain organization and how it is related to mitochondrial morphology.
History
DepositionSep 23, 2022Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jun 14, 2023Provider: repository / Type: Initial release

-
Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

-
Assembly

Deposited unit
1t: Tim10/DDP family zinc finger protein
2e: NmrA domain-containing protein
2f: Transmembrane protein, putative
2g: SDHTT3
2h: Diphthamide synthesis protein
2i: DUF4885 domain-containing protein
2j: Transmembrane protein, putative
2k: Transmembrane protein, putative
2l: Transposase
2m: Transmembrane protein, putative
2n: Transmembrane protein, putative
2o: SDHTT11
2t: Zf-Tim10_DDP domain-containing protein
3t: Zf-Tim10_DDP domain-containing protein
4a: Phage protein
4t: Transposase
5t: Cullin domain-containing protein
6a: Transmembrane protein, putative
6b: Structural protein
6c: Transmembrane protein, putative
6l: Decapping nuclease
6t: Annexin
7a: Transmembrane protein, putative
7c: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
7l: CTF/NF-I domain-containing protein
a: Transmembrane protein, putative
b: Protein phosphatase 2C, putative
bp: Chromosome condensation regulator RCC1 repeat protein
c1: Cytochrome c oxidase subunit 1
c2: Cytochrome c oxidase subunit 2
c3: Ymf68
d: SURF1-like protein
e: TraB family protein
f: Transmembrane protein, putative
fs: Iron-binding zinc finger CDGSH type protein
g: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, mitochondrial
h: SURF1-like protein
i: COXTT9
j: COXTT10
k: 39S ribosomal protein L9, mitochondrial
l: Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C
m: Transmembrane protein, putative
m1: Oxoglutarate/malate translocator protein, putative
m2: 2-oxoglutarate/malate carrier protein
m3: Carrier protein
n: Transmembrane protein, putative
o: Mobilization protein
p: YflT domain-containing protein
q: Transmembrane protein, putative
r: Transmembrane protein
s: Complex III subunit VII
sa: Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
sb: Succinate dehydrogenase (quinone)
sc: Cytochrome b-c1 complex subunit 8
sd: SDHD
t: Transmembrane protein, putative
u: Transmembrane protein, putative
v: COXTT22
vb: Cytochrome C oxidase subunit Vb protein
w: Transmembrane protein, putative
x: Transmembrane protein, putative
y: Lysozyme
y0: Ymf70
y5: Ymf75
y7: Ymf67
z: ABC transporter
z1: COXTT28
1b: NADH dehydrogenase subunit 1
2b: NADH dehydrogenase subunit 2
4l: Ymf58
5b: Ymf57
a1: Transmembrane protein, putative
a2: Ribosomal protein L51/S25/CI-B8 domain protein
a3: Transmembrane protein, putative
a5: ETC complex I subunit motif protein
a6: NADH dehydrogenase, putative
a7: 37S ribosomal protein S25, mitochondrial
a8: CX9C domain-containing protein
a9: NAD-dependent epimerase/dehydratase family protein
ab: Acyl carrier protein
ac: Acyl carrier protein
al: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
am: NDUA13
an: Transmembrane protein, putative
b2: NDUB2
b3: Transmembrane protein, putative
b4: NDUB4
b6: NDUB6
b7: CHCH domain-containing protein
b8: NDUB8
b9: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
bl: NDUB10
bm: Transmembrane protein, putative
c4: Complex I-MNLL
fx: 2 iron, 2 sulfur cluster-binding protein
g1: Gamma-carbonic anhydrase
g2: Gamma-carbonic anhydrase
g3: Transcription factor apfi protein, putative
j1: DnaJ domain protein
n1: NADH-ubiquinone oxidoreductase chain 1
n2: Ymf65
n3: NADH-ubiquinone oxidoreductase chain 3
n4: NADH-ubiquinone oxidoreductase chain 4
n5: NADH dehydrogenase subunit 5
n6: Ymf62
p1: Transmembrane protein, putative
p2: NDUPH2
qA: M16 family peptidase, putative
qB: Peptidase M16 inactive domain protein
qC: Apocytochrome b
qD: Cytochrome protein c1
qE: Rieske iron-sulfur protein, ubiquinol-cytochrome C reductase iron-sulfur subunit
qF: Ubiquinol-cytochrome C reductase hinge protein
qG: Sulphotransf domain-containing protein
qH: Transmembrane protein, putative
qI: Transmembrane protein, putative
qJ: Transmembrane protein, putative
qL: UQCRTT2
qM: Unknown peptide
qa: M16 family peptidase, putative
qb: Peptidase M16 inactive domain protein
qc: Apocytochrome b
qd: Cytochrome protein c1
qe: Rieske iron-sulfur protein, ubiquinol-cytochrome C reductase iron-sulfur subunit
qf: Ubiquinol-cytochrome C reductase hinge protein
qg: Sulphotransf domain-containing protein
qh: Transmembrane protein, putative
qi: Transmembrane protein, putative
qj: Transmembrane protein, putative
ql: UQCRTT2
qm: Unknown peptide
s1: NADH-ubiquinone oxidoreductase 75 kDa subunit
s2: NADH dehydrogenase subunit 7
s3: NADH dehydrogenase subunit 9
s4: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
s5: GRAM domain protein
s6: Zinc-finger protein
s7: NADH dehydrogenase subunit 10
s8: NADH-ubiquinone oxidoreductase 1, chain, putative
t1: Lipid-A-disaccharide synthase
t2: Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II
t3: RNase III domain-containing protein
t4: Transmembrane protein
t5: Transmembrane protein, putative
t6: COX assembly mitochondrial protein
t7: Transmembrane protein, putative
t8: PH domain-containing protein
t9: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
ta: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
tb: Transmembrane protein, putative
tc: ATP synthase subunit e, mitochondrial
td: Transmembrane protein, putative
te: Transmembrane protein, putative
tf: NDUTT15
tg: NDUTT16
th: NDUTT17
tx: Thioredoxin
v1: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
v2: NADH-ubiquinone oxidoreductase 24 kDa subunit
x1: NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit
1T: Tim10/DDP family zinc finger protein
2E: NmrA domain-containing protein
2F: Transmembrane protein, putative
2G: SDHTT3
2H: Diphthamide synthesis protein
2I: DUF4885 domain-containing protein
2J: Transmembrane protein, putative
2K: Transmembrane protein, putative
2L: Transposase
2M: Transmembrane protein, putative
2N: Transmembrane protein, putative
2O: SDHTT11
2T: Zf-Tim10_DDP domain-containing protein
3T: Zf-Tim10_DDP domain-containing protein
4A: Phage protein
4T: Transposase
5T: Cullin domain-containing protein
6A: Transmembrane protein, putative
6B: Structural protein
6C: Transmembrane protein, putative
6L: Decapping nuclease
6T: Annexin
7A: Transmembrane protein, putative
7C: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
7L: CTF/NF-I domain-containing protein
A: Transmembrane protein, putative
B: Protein phosphatase 2C, putative
BP: Chromosome condensation regulator RCC1 repeat protein
C1: Cytochrome c oxidase subunit 1
C2: Cytochrome c oxidase subunit 2
C3: Ymf68
D: SURF1-like protein
E: TraB family protein
F: Transmembrane protein, putative
FS: Iron-binding zinc finger CDGSH type protein
G: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, mitochondrial
H: SURF1-like protein
I: COXTT9
J: COXTT10
K: 39S ribosomal protein L9, mitochondrial
L: Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C
M: Transmembrane protein, putative
M1: Oxoglutarate/malate translocator protein, putative
M2: 2-oxoglutarate/malate carrier protein
M3: Carrier protein
N: Transmembrane protein, putative
O: Mobilization protein
P: YflT domain-containing protein
Q: Transmembrane protein, putative
R: Transmembrane protein
S: Complex III subunit VII
SA: Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
SB: Succinate dehydrogenase (quinone)
SC: Cytochrome b-c1 complex subunit 8
SD: SDHD
T: Transmembrane protein, putative
U: Transmembrane protein, putative
V: COXTT22
VB: Cytochrome C oxidase subunit Vb protein
W: Transmembrane protein, putative
X: Transmembrane protein, putative
Y: Lysozyme
Y0: Ymf70
Y5: Ymf75
Y7: Ymf67
Z: ABC transporter
Z1: COXTT28
1B: NADH dehydrogenase subunit 1
2B: NADH dehydrogenase subunit 2
4L: Ymf58
5B: Ymf57
A1: Transmembrane protein, putative
A2: Ribosomal protein L51/S25/CI-B8 domain protein
A3: Transmembrane protein, putative
A5: ETC complex I subunit motif protein
A6: NADH dehydrogenase, putative
A7: 37S ribosomal protein S25, mitochondrial
A8: CX9C domain-containing protein
A9: NAD-dependent epimerase/dehydratase family protein
AB: Acyl carrier protein
AC: Acyl carrier protein
AL: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
AM: NDUA13
AN: Transmembrane protein, putative
B2: NDUB2
B3: Transmembrane protein, putative
B4: NDUB4
B6: NDUB6
B7: CHCH domain-containing protein
B8: NDUB8
B9: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
BL: NDUB10
BM: Transmembrane protein, putative
C4: Complex I-MNLL
FX: 2 iron, 2 sulfur cluster-binding protein
G1: Gamma-carbonic anhydrase
G2: Gamma-carbonic anhydrase
G3: Transcription factor apfi protein, putative
J1: DnaJ domain protein
N1: NADH-ubiquinone oxidoreductase chain 1
N2: Ymf65
N3: NADH-ubiquinone oxidoreductase chain 3
N4: NADH-ubiquinone oxidoreductase chain 4
N5: NADH dehydrogenase subunit 5
N6: Ymf62
P1: Transmembrane protein, putative
P2: NDUPH2
QA: M16 family peptidase, putative
QB: Peptidase M16 inactive domain protein
QC: Apocytochrome b
QD: Cytochrome protein c1
QE: Rieske iron-sulfur protein, ubiquinol-cytochrome C reductase iron-sulfur subunit
QF: Ubiquinol-cytochrome C reductase hinge protein
QG: Sulphotransf domain-containing protein
QH: Transmembrane protein, putative
QI: Transmembrane protein, putative
QJ: Transmembrane protein, putative
QL: UQCRTT2
Qa: M16 family peptidase, putative
Qb: Peptidase M16 inactive domain protein
Qc: Apocytochrome b
Qd: Cytochrome protein c1
Qe: Rieske iron-sulfur protein, ubiquinol-cytochrome C reductase iron-sulfur subunit
Qf: Ubiquinol-cytochrome C reductase hinge protein
Qg: Sulphotransf domain-containing protein
Qh: Transmembrane protein, putative
Qi: Transmembrane protein, putative
Qj: Transmembrane protein, putative
Ql: UQCRTT2
Qm: Unknown peptide
S1: NADH-ubiquinone oxidoreductase 75 kDa subunit
S2: NADH dehydrogenase subunit 7
S3: NADH dehydrogenase subunit 9
S4: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
S5: GRAM domain protein
S6: Zinc-finger protein
S7: NADH dehydrogenase subunit 10
S8: NADH-ubiquinone oxidoreductase 1, chain, putative
T1: Lipid-A-disaccharide synthase
T2: Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II
T3: RNase III domain-containing protein
T4: Transmembrane protein
T5: Transmembrane protein, putative
T6: COX assembly mitochondrial protein
T7: Transmembrane protein, putative
T8: PH domain-containing protein
T9: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
TA: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
TB: Transmembrane protein, putative
TC: ATP synthase subunit e, mitochondrial
TD: Transmembrane protein, putative
TE: Transmembrane protein, putative
TF: NDUTT15
TG: NDUTT16
TH: NDUTT17
TX: Thioredoxin
V1: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
V2: NADH-ubiquinone oxidoreductase 24 kDa subunit
X1: NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit
C: COXTT3
c: COXTT3
u1: Unknown peptide
u2: Unknown peptide
U2: Unknown peptide
QM: Unknown peptide
U1: Unknown peptide
hetero molecules


Theoretical massNumber of molelcules
Total (without water)9,057,423836
Polymers8,555,282326
Non-polymers502,142510
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551

-
Components

+
Protein , 79 types, 170 molecules 1t1T2e2E2g2G2h2H2i2I2l2L4a4A4t4T5t5T6b6B6l6L6t6T7l7LbBbpBP...

#1: Protein Tim10/DDP family zinc finger protein


Mass: 8008.990 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: W7X3D6
#2: Protein NmrA domain-containing protein


Mass: 36376.008 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q24I09
#4: Protein SDHTT3


Mass: 23627.730 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MEX7
#5: Protein Diphthamide synthesis protein


Mass: 22894.336 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7LX66
#6: Protein DUF4885 domain-containing protein


Mass: 12979.428 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22YL0
#9: Protein Transposase


Mass: 11027.419 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: W7XBF5
#15: Protein Phage protein


Mass: 14740.897 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q24C97
#16: Protein Transposase


Mass: 8081.111 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22A35
#17: Protein Cullin domain-containing protein


Mass: 8965.917 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22N23
#19: Protein Structural protein


Mass: 27946.445 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q24I72
#21: Protein Decapping nuclease


Mass: 10200.682 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7LVX0
#22: Protein Annexin


Mass: 8155.360 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q233U0
#25: Protein CTF/NF-I domain-containing protein


Mass: 116812.750 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22CI1
#27: Protein Protein phosphatase 2C, putative


Mass: 53011.297 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22FX8
#28: Protein Chromosome condensation regulator RCC1 repeat protein


Mass: 50912.336 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
#31: Protein Ymf68


Mass: 72782.539 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q950Y6
#32: Protein SURF1-like protein


Mass: 47087.973 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M1Q4
#33: Protein TraB family protein


Mass: 45022.914 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23DP7
#35: Protein Iron-binding zinc finger CDGSH type protein


Mass: 21476.887 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M8P0
#37: Protein SURF1-like protein


Mass: 35794.367 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7LTZ4
#38: Protein COXTT9


Mass: 29865.686 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7LY65
#39: Protein COXTT10


Mass: 28101.486 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MD70
#40: Protein 39S ribosomal protein L9, mitochondrial


Mass: 27034.711 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: W7X4J9
#43: Protein Oxoglutarate/malate translocator protein, putative


Mass: 40392.234 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: A4VDV3
#44: Protein 2-oxoglutarate/malate carrier protein


Mass: 36090.277 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23M99
#45: Protein Carrier protein


Mass: 37095.141 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22ZA6
#47: Protein Mobilization protein


Mass: 23257.660 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23F08
#48: Protein YflT domain-containing protein


Mass: 19979.320 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M8Y9
#51: Protein Complex III subunit VII


Mass: 19882.758 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q230X6
#54: Protein Cytochrome b-c1 complex subunit 8


Mass: 7036.020 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23RH8
#58: Protein COXTT22


Mass: 17701.115 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MFV5
#62: Protein Lysozyme


Mass: 12273.913 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M9E7
#63: Protein Ymf70


Mass: 10891.920 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q950Y0
#64: Protein Ymf75


Mass: 23325.801 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q951A7
#65: Protein Ymf67


Mass: 54538.531 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q950Y7
#66: Protein ABC transporter


Mass: 10418.697 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7LTF1
#67: Protein COXTT28


Mass: 11121.555 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
#70: Protein Ymf58


Mass: 13509.949 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q950Z5
#71: Protein Ymf57


Mass: 12473.938 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q951C2
#73: Protein Ribosomal protein L51/S25/CI-B8 domain protein


Mass: 12157.782 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MA77
#75: Protein ETC complex I subunit motif protein


Mass: 24277.859 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23ND5
#76: Protein NADH dehydrogenase, putative


Mass: 20167.854 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M2Y3
#77: Protein 37S ribosomal protein S25, mitochondrial


Mass: 33201.797 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MIJ7
#78: Protein CX9C domain-containing protein


Mass: 28412.805 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MMF4
#79: Protein NAD-dependent epimerase/dehydratase family protein


Mass: 41375.090 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MLH2
#83: Protein NDUA13


Mass: 21045.617 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M2U4
#85: Protein NDUB2


Mass: 14410.395 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MG29
#87: Protein NDUB4


Mass: 17502.564 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
#88: Protein NDUB6


Mass: 15444.344 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q231G0
#89: Protein CHCH domain-containing protein


Mass: 13747.362 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MIM0
#90: Protein NDUB8


Mass: 24150.469 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M855
#92: Protein NDUB10


Mass: 22167.137 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23KG0
#94: Protein Complex I-MNLL


Mass: 12103.036 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22W63
#95: Protein 2 iron, 2 sulfur cluster-binding protein


Mass: 19564.221 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22W11
#98: Protein Transcription factor apfi protein, putative


Mass: 39793.414 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M8Q7
#99: Protein DnaJ domain protein


Mass: 36878.367 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22DR7
#101: Protein Ymf65


Mass: 43104.082 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q951A3
#105: Protein Ymf62


Mass: 30448.471 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q950Y2
#107: Protein NDUPH2


Mass: 22446.637 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23KE0
#108: Protein
M16 family peptidase, putative


Mass: 53030.543 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MJ25
#109: Protein
Peptidase M16 inactive domain protein


Mass: 58023.914 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MGU2
#110: Protein
Apocytochrome b


Mass: 50635.547 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q950Z1, quinol-cytochrome-c reductase
#111: Protein
Cytochrome protein c1


Mass: 37616.629 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q24IM5
#112: Protein
Rieske iron-sulfur protein, ubiquinol-cytochrome C reductase iron-sulfur subunit


Mass: 30696.961 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MIC7
#114: Protein
Sulphotransf domain-containing protein


Mass: 39193.523 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23F81
#124: Protein GRAM domain protein


Mass: 10920.431 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: W7X4R4
#125: Protein Zinc-finger protein


Mass: 15141.296 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MK02
#128: Protein Lipid-A-disaccharide synthase


Mass: 59307.289 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22E24, lipid-A-disaccharide synthase
#129: Protein Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II


Mass: 36709.441 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q24HK5
#130: Protein RNase III domain-containing protein


Mass: 35409.246 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote) / References: UniProt: I7LUQ4
#133: Protein COX assembly mitochondrial protein


Mass: 17078.342 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M1N8
#135: Protein PH domain-containing protein


Mass: 15785.234 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22SC4
#139: Protein ATP synthase subunit e, mitochondrial


Mass: 11283.534 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22E95
#142: Protein NDUTT15


Mass: 27562.686 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
#143: Protein NDUTT16


Mass: 15465.568 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
#144: Protein NDUTT17


Mass: 14124.288 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
#145: Protein Thioredoxin


Mass: 19540.191 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22AI5
#149: Protein COXTT3


Mass: 23368.416 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
#150: Protein Unknown peptide


Mass: 7847.665 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences

+
Transmembrane ... , 33 types, 72 molecules 2f2F2j2J2k2K2m2M2n2N6a6A6c6C7a7AaAfFmMnNqQrRtT...

#3: Protein Transmembrane protein, putative


Mass: 35124.293 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q248F8
#7: Protein Transmembrane protein, putative


Mass: 11957.518 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23S01
#8: Protein Transmembrane protein, putative


Mass: 11188.824 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q24CW6
#10: Protein Transmembrane protein, putative


Mass: 9168.366 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22HD6
#11: Protein Transmembrane protein, putative


Mass: 7244.434 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: W7XF00
#18: Protein Transmembrane protein, putative


Mass: 15618.258 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: W7XCY5
#20: Protein Transmembrane protein, putative


Mass: 12786.321 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23DS4
#23: Protein Transmembrane protein, putative


Mass: 16385.635 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MGF9
#26: Protein Transmembrane protein, putative


Mass: 57814.465 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22PJ5
#34: Protein Transmembrane protein, putative


Mass: 41307.387 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23DG8
#42: Protein Transmembrane protein, putative


Mass: 26601.174 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: W7WZP1
#46: Protein Transmembrane protein, putative


Mass: 24701.131 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7LZX8
#49: Protein Transmembrane protein, putative


Mass: 20346.227 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23D87
#50: Protein Transmembrane protein


Mass: 20025.109 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MKT6
#56: Protein Transmembrane protein, putative


Mass: 18801.604 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23VY4
#57: Protein Transmembrane protein, putative


Mass: 18600.445 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22DP8
#60: Protein Transmembrane protein, putative


Mass: 15588.598 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23TE5
#61: Protein Transmembrane protein, putative


Mass: 14302.487 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22W32
#72: Protein Transmembrane protein, putative


Mass: 11451.183 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MI60
#74: Protein Transmembrane protein, putative


Mass: 15831.701 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M9B3
#84: Protein Transmembrane protein, putative


Mass: 26545.146 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q24F24
#86: Protein Transmembrane protein, putative


Mass: 9942.366 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: A4VD20
#93: Protein Transmembrane protein, putative


Mass: 24419.150 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22Z32
#106: Protein Transmembrane protein, putative


Mass: 29389.660 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q24C39
#115: Protein
Transmembrane protein, putative


Mass: 15677.771 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M484
#116: Protein
Transmembrane protein, putative


Mass: 14262.554 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MM45
#117: Protein
Transmembrane protein, putative


Mass: 7455.741 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MFL6
#131: Protein Transmembrane protein


Mass: 24823.297 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MIE0
#132: Protein Transmembrane protein, putative


Mass: 23971.033 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7LT77
#134: Protein Transmembrane protein, putative


Mass: 16909.242 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22HE4
#138: Protein Transmembrane protein, putative


Mass: 13340.979 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22T55
#140: Protein Transmembrane protein, putative


Mass: 8787.116 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22DC2
#141: Protein Transmembrane protein, putative


Mass: 8445.937 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MIK1

+
Protein/peptide , 4 types, 14 molecules 2o2OsdSDqLqlQLQlqMqmQmu2U2QM

#12: Protein/peptide SDHTT11


Mass: 5459.540 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
#55: Protein/peptide SDHD


Mass: 5553.692 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
#118: Protein/peptide
UQCRTT2


Mass: 4850.612 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
#119: Protein/peptide
Unknown peptide


Mass: 1464.797 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences

+
Zf-Tim10 DDP domain-containing ... , 2 types, 4 molecules 2t2T3t3T

#13: Protein Zf-Tim10_DDP domain-containing protein


Mass: 8354.574 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: W7XDM6
#14: Protein Zf-Tim10_DDP domain-containing protein


Mass: 10435.701 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q231A8

+
NADH dehydrogenase [ubiquinone] ... , 8 types, 16 molecules 7c7CgGalALb9B9s4S4t9T9taTAv1V1

#24: Protein NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial


Mass: 28666.662 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: W7X287
#36: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, mitochondrial


Mass: 37552.980 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23DZ5
#82: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12


Mass: 22890.570 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: A4VDQ6
#91: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial


Mass: 22698.301 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q233X7
#123: Protein NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial


Mass: 21642.377 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MK61
#136: Protein NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8


Mass: 15689.792 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23B10
#137: Protein NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4


Mass: 15193.583 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MAF0
#146: Protein NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial


Mass: 52313.699 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23KE4, NADH:ubiquinone reductase (H+-translocating)

+
Cytochrome c oxidase subunit ... , 3 types, 6 molecules c1C1c2C2vbVB

#29: Protein Cytochrome c oxidase subunit 1


Mass: 80564.945 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q950Y4, cytochrome-c oxidase
#30: Protein Cytochrome c oxidase subunit 2


Mass: 72388.883 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q950Y9, cytochrome-c oxidase
#59: Protein Cytochrome C oxidase subunit Vb protein


Mass: 75431.312 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23FF5

+
Ubiquinol-cytochrome ... , 2 types, 6 molecules lLqFqfQFQf

#41: Protein Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C


Mass: 26738.438 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M3P9
#113: Protein
Ubiquinol-cytochrome C reductase hinge protein


Mass: 10249.956 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22AX2

+
Succinate dehydrogenase ... , 2 types, 4 molecules saSAsbSB

#52: Protein Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial


Mass: 70082.758 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23DI3
#53: Protein Succinate dehydrogenase (quinone) / Iron-sulfur subunit of complex II


Mass: 36002.402 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M403, succinate dehydrogenase

+
NADH dehydrogenase subunit ... , 6 types, 12 molecules 1b1B2b2Bn5N5s2S2s3S3s7S7

#68: Protein NADH dehydrogenase subunit 1


Mass: 7238.754 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q09FB0
#69: Protein NADH dehydrogenase subunit 2


Mass: 20899.889 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q951B2, NADH:ubiquinone reductase (H+-translocating)
#104: Protein NADH dehydrogenase subunit 5


Mass: 88179.844 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q950Z0, NADH:ubiquinone reductase (H+-translocating)
#121: Protein NADH dehydrogenase subunit 7


Mass: 51227.477 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q951B1, NADH:ubiquinone reductase (H+-translocating)
#122: Protein NADH dehydrogenase subunit 9


Mass: 23803.268 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q950Z4, NADH:ubiquinone reductase (H+-translocating)
#126: Protein NADH dehydrogenase subunit 10


Mass: 18324.260 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q951B4, NADH:ubiquinone reductase (H+-translocating)

+
Acyl carrier ... , 2 types, 4 molecules abABacAC

#80: Protein Acyl carrier protein


Mass: 16249.438 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22XT6
#81: Protein Acyl carrier protein


Mass: 15428.433 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MD12

+
Gamma-carbonic ... , 2 types, 4 molecules g1G1g2G2

#96: Protein Gamma-carbonic anhydrase


Mass: 28216.734 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q22XU5
#97: Protein Gamma-carbonic anhydrase


Mass: 25395.969 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7M6S0

+
NADH-ubiquinone oxidoreductase ... , 7 types, 14 molecules n1N1n3N3n4N4s1S1s8S8v2V2x1X1

#100: Protein NADH-ubiquinone oxidoreductase chain 1


Mass: 32636.770 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q950Y3, NADH:ubiquinone reductase (H+-translocating)
#102: Protein NADH-ubiquinone oxidoreductase chain 3


Mass: 14416.898 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q950Z7, NADH:ubiquinone reductase (H+-translocating)
#103: Protein NADH-ubiquinone oxidoreductase chain 4


Mass: 58690.043 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q950X9, NADH:ubiquinone reductase (H+-translocating)
#120: Protein NADH-ubiquinone oxidoreductase 75 kDa subunit


Mass: 80580.789 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: Q23KA9
#127: Protein NADH-ubiquinone oxidoreductase 1, chain, putative


Mass: 28053.535 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MDW5
#147: Protein NADH-ubiquinone oxidoreductase 24 kDa subunit


Mass: 31216.697 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7MEP0
#148: Protein NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit


Mass: 17381.809 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Tetrahymena thermophila SB210 (eukaryote)
Plasmid details: Institute of Hydrobiology, Chinese Academy of Sciences
References: UniProt: I7LT42

+
Non-polymers , 21 types, 510 molecules

#151: Chemical
ChemComp-HEC / HEME C


Mass: 618.503 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C34H34FeN4O4 / Feature type: SUBJECT OF INVESTIGATION
#152: Chemical...
ChemComp-PC1 / 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / 3-SN-PHOSPHATIDYLCHOLINE


Mass: 790.145 Da / Num. of mol.: 168 / Source method: obtained synthetically / Formula: C44H88NO8P / Comment: phospholipid*YM
#153: Chemical...
ChemComp-CDL / CARDIOLIPIN / DIPHOSPHATIDYL GLYCEROL / BIS-(1,2-DIACYL-SN-GLYCERO-3-PHOSPHO)-1',3'-SN-GLYCEROL


Mass: 1464.043 Da / Num. of mol.: 198 / Source method: obtained synthetically / Formula: C81H156O17P2 / Comment: phospholipid*YM
#154: Chemical
ChemComp-HEA / HEME-A


Mass: 852.837 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C49H56FeN4O6 / Feature type: SUBJECT OF INVESTIGATION
#155: Chemical
ChemComp-CU / COPPER (II) ION


Mass: 63.546 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Cu / Feature type: SUBJECT OF INVESTIGATION
#156: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
#157: Chemical
ChemComp-FES / FE2/S2 (INORGANIC) CLUSTER


Mass: 175.820 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: Fe2S2 / Feature type: SUBJECT OF INVESTIGATION
#158: Chemical...
ChemComp-3PE / 1,2-Distearoyl-sn-glycerophosphoethanolamine / 3-SN-PHOSPHATIDYLETHANOLAMINE / 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE


Mass: 748.065 Da / Num. of mol.: 48 / Source method: obtained synthetically / Formula: C41H82NO8P / Comment: phospholipid*YM
#159: Chemical ChemComp-P5S / O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine / phosphatidyl serine


Mass: 792.075 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C42H82NO10P
#160: Chemical
ChemComp-PX2 / 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE


Mass: 535.671 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C27H52O8P
#161: Chemical ChemComp-FAD / FLAVIN-ADENINE DINUCLEOTIDE


Mass: 785.550 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C27H33N9O15P2 / Feature type: SUBJECT OF INVESTIGATION / Comment: FAD*YM
#162: Chemical
ChemComp-SF4 / IRON/SULFUR CLUSTER


Mass: 351.640 Da / Num. of mol.: 14 / Source method: obtained synthetically / Formula: Fe4S4
#163: Chemical ChemComp-F3S / FE3-S4 CLUSTER


Mass: 295.795 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Fe3S4
#164: Chemical
ChemComp-U10 / UBIQUINONE-10 / Coenzyme Q10


Mass: 863.343 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: C59H90O4 / Feature type: SUBJECT OF INVESTIGATION
#165: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Zn
#166: Chemical
ChemComp-HEM / PROTOPORPHYRIN IX CONTAINING FE / HEME


Mass: 616.487 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: C34H32FeN4O4 / Feature type: SUBJECT OF INVESTIGATION
#167: Chemical ChemComp-NDP / NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE


Mass: 745.421 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C21H30N7O17P3
#168: Chemical ChemComp-ZMP / S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate


Mass: 568.704 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C25H49N2O8PS / Feature type: SUBJECT OF INVESTIGATION
#169: Chemical ChemComp-T7X / Phosphatidylinositol


Mass: 887.128 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C47H83O13P
#170: Chemical ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE


Mass: 427.201 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H15N5O10P2 / Comment: ADP, energy-carrying molecule*YM
#171: Chemical ChemComp-FMN / FLAVIN MONONUCLEOTIDE / RIBOFLAVIN MONOPHOSPHATE


Mass: 456.344 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C17H21N4O9P / Feature type: SUBJECT OF INVESTIGATION

+
Details

Has ligand of interestY

-
Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

-
Sample preparation

ComponentName: Tetrahymena thermophila respiratory mega-complex MC (IV2+I+III2+II)2
Type: COMPLEX / Entity ID: #1-#150 / Source: NATURAL
Molecular weightValue: 8 MDa / Experimental value: NO
Source (natural)Organism: Tetrahymena thermophila SB210 (eukaryote)
Buffer solutionpH: 7.4
Details: SEC buffer (20 mM Tris pH 7.4, 50 mM NaCl, 0.002% PMSF, 0.005% LMNG (w/v))
Buffer component
IDConc.NameFormulaBuffer-ID
120 mMTrimethylsilylTris1
250 mMSodium chlorideNaCl1
30.002 %Phenylmethylsulfonyl fluoridePMSF1
40.005 %lauryl maltose neopentyl glycolLMNG1
SpecimenConc.: 4 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportDetails: 25 mA / Grid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R0.6/1
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277.15 K

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 140000 X / Nominal defocus max: 2000 nm / Nominal defocus min: 800 nm / Cs: 2.7 mm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingAverage exposure time: 7 sec. / Electron dose: 61.5 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) / Num. of grids imaged: 2 / Num. of real images: 16772
Image scansWidth: 4096 / Height: 4096

-
Processing

Software
NameVersionClassificationNB
phenix.real_space_refine1.20.1_4487refinement
PHENIX1.20.1_4487refinement
EM software
IDNameVersionCategory
1crYOLO1.6.0particle selection
2EPUimage acquisition
4CTFFIND4.1CTF correction
7PHENIX1.20.1model fitting
8Coot0.9.6model fitting
10cryoSPARCv3.3.2initial Euler assignment
11cryoSPARCv3.3.2final Euler assignment
12cryoSPARCv3.3.2classification
13cryoSPARCv3.3.23D reconstruction
14PHENIX1.20.1model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selectionNum. of particles selected: 1220892
SymmetryPoint symmetry: C2 (2 fold cyclic)
3D reconstructionResolution: 2.96 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 97688 / Algorithm: FOURIER SPACE / Num. of class averages: 1 / Symmetry type: POINT
Atomic model buildingProtocol: RIGID BODY FIT / Space: REAL
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 56.79 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0022582734
ELECTRON MICROSCOPYf_angle_d0.4561785758
ELECTRON MICROSCOPYf_chiral_restr0.039581536
ELECTRON MICROSCOPYf_plane_restr0.003598122
ELECTRON MICROSCOPYf_dihedral_angle_d11.1596223300

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbjlvh1.pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more