+検索条件
-Structure paper
タイトル | Structures of Tetrahymena thermophila respiratory megacomplexes on the tubular mitochondrial cristae. |
---|---|
ジャーナル・号・ページ | Nat Commun, Vol. 14, Issue 1, Page 2542, Year 2023 |
掲載日 | 2023年5月29日 |
著者 | Fangzhu Han / Yiqi Hu / Mengchen Wu / Zhaoxiang He / Hongtao Tian / Long Zhou / |
PubMed 要旨 | Tetrahymena thermophila, a classic ciliate model organism, has been shown to possess tubular mitochondrial cristae and highly divergent electron transport chain involving four transmembrane protein ...Tetrahymena thermophila, a classic ciliate model organism, has been shown to possess tubular mitochondrial cristae and highly divergent electron transport chain involving four transmembrane protein complexes (I-IV). Here we report cryo-EM structures of its ~8 MDa megacomplex IV+ (I + III+ II), as well as a ~ 10.6 MDa megacomplex (IV + I + III+ II) at lower resolution. In megacomplex IV+ (I + III+ II), each CIV protomer associates one copy of supercomplex I + III and one copy of CII, forming a half ring-shaped architecture that adapts to the membrane curvature of mitochondrial cristae. Megacomplex (IV+ I + III+ II) defines the relative position between neighbouring half rings and maintains the proximity between CIV and CIII cytochrome c binding sites. Our findings expand the current understanding of divergence in eukaryotic electron transport chain organization and how it is related to mitochondrial morphology. |
リンク | Nat Commun / PubMed:37248254 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.8 - 6.77 Å |
構造データ | EMDB-34373, PDB-8gym: EMDB-34380: Cryo-EM map of Tetrahymena thermophila respiratory complex I peripheral arm EMDB-34381: Cryo-EM map of Tetrahymena thermophila respiratory complex I membrane arm distal portion EMDB-34382: Cryo-EM map of Tetrahymena thermophila respiratory complex I membrane arm proximal portion EMDB-34383: Cryo-EM map of Tetrahymena thermophila respiratory complex IV EMDB-34384: Cryo-EM map of Tetrahymena thermophila respiratory complex III EMDB-34385: Cryo-EM map of Tetrahymena thermophila respiratory complex II EMDB-34386: Cryo-EM map of Tetrahymena thermophila respiratory complex I membrane arm distal portion, digitonin sample EMDB-34403, PDB-8gzu: EMDB-34404: Cryo-EM structure of Tetrahymena thermophila respiratory super-complex SC IV2+I+III2+II EMDB-34405: Cryo-EM structure of Tetrahymena thermophila respiratory super-complex SC IV2+I+III2+II, copy2 EMDB-36375: Cryo-EM map of Tetrahymena thermophila megacomplex IV2+(I+III2+II)2 |
化合物 | ChemComp-HEC: ChemComp-PC1: ChemComp-CDL: ChemComp-HEA: ChemComp-CU: ChemComp-MG: ChemComp-FES: ChemComp-3PE: ChemComp-P5S: ChemComp-PX2: ChemComp-FAD: ChemComp-SF4: ChemComp-F3S: ChemComp-U10: ChemComp-ZN: ChemComp-HEM: ChemComp-NDP: ChemComp-ZMP: ChemComp-T7X: ChemComp-ADP: ChemComp-FMN: |
由来 |
|
キーワード | ELECTRON TRANSPORT (電子伝達系) / Electron transport chain (電子伝達系) / supercomplex / membrane protein (膜タンパク質) / Tetrahymena thermophila |