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- PDB-7rye: Cryo-EM structure of the needle filament-tip complex of the Salmo... -

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Basic information

Entry
Database: PDB / ID: 7rye
TitleCryo-EM structure of the needle filament-tip complex of the Salmonella type III secretion injectisome
Components
  • Cell invasion protein SipD
  • Protein PrgI
KeywordsCELL INVASION / protein secretion / bacterial pathogenesis / organelle assembly
Function / homology
Function and homology information


type III protein secretion system complex / protein secretion by the type III secretion system / : / cell surface / extracellular region / identical protein binding
Similarity search - Function
Type III secretion systems tip complex components / BipD-like superfamily / Type III secretion systems tip complex components / Type III secretion, needle-protein-like / Type III secretion, needle-protein-like superfamily / Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF / Type III secretion system, needle protein
Similarity search - Domain/homology
Cell invasion protein SipD / SPI-1 type 3 secretion system needle filament protein
Similarity search - Component
Biological speciesSalmonella enterica subsp. enterica serovar Typhimurium (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.9 Å
AuthorsGuo, E.Z. / Galan, J.E.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI030492 United States
CitationJournal: Proc Natl Acad Sci U S A / Year: 2021
Title: Cryo-EM structure of the needle filament tip complex of the type III secretion injectisome.
Authors: Emily Z Guo / Jorge E Galán /
Abstract: Type III secretion systems are multiprotein molecular machines required for the virulence of several important bacterial pathogens. The central element of these machines is the injectisome, a ∼5-Md ...Type III secretion systems are multiprotein molecular machines required for the virulence of several important bacterial pathogens. The central element of these machines is the injectisome, a ∼5-Md multiprotein structure that mediates the delivery of bacterially encoded proteins into eukaryotic target cells. The injectisome is composed of a cytoplasmic sorting platform, and a membrane-embedded needle complex, which is made up of a multiring base and a needle-like filament that extends several nanometers from the bacterial surface. The needle filament is capped at its distal end by another substructure known as the tip complex, which is crucial for the translocation of effector proteins through the eukaryotic cell plasma membrane. Here we report the cryo-EM structure of the Typhimurium needle tip complex docked onto the needle filament tip. Combined with a detailed analysis of structurally guided mutants, this study provides major insight into the assembly and function of this essential component of the type III secretion protein injection machine.
History
DepositionAug 25, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 10, 2021Provider: repository / Type: Initial release
Revision 1.1Jun 5, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

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  • Deposited structure unit
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  • EMDB-24735
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Assembly

Deposited unit
A: Protein PrgI
B: Protein PrgI
C: Protein PrgI
D: Protein PrgI
E: Protein PrgI
F: Cell invasion protein SipD
G: Protein PrgI
H: Protein PrgI
I: Protein PrgI
J: Protein PrgI
K: Protein PrgI
L: Protein PrgI
M: Protein PrgI
N: Protein PrgI
O: Cell invasion protein SipD
P: Protein PrgI
Q: Protein PrgI
R: Cell invasion protein SipD
S: Protein PrgI
T: Protein PrgI
U: Protein PrgI
V: Cell invasion protein SipD
W: Protein PrgI
X: Cell invasion protein SipD


Theoretical massNumber of molelcules
Total (without water)354,13824
Polymers354,13824
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: mass spectrometry
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area77680 Å2
ΔGint-441 kcal/mol
Surface area101560 Å2

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Components

#1: Protein
Protein PrgI


Mass: 8864.868 Da / Num. of mol.: 19 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium (bacteria)
References: UniProt: P41784
#2: Protein
Cell invasion protein SipD / SPI-1 type III secretion system needle tip complex protein SipD / Translocation machinery component


Mass: 37141.148 Da / Num. of mol.: 5 / Source method: isolated from a natural source
Source: (natural) Salmonella enterica subsp. enterica serovar Typhimurium (bacteria)
References: UniProt: A0A0C5PQX9

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: The needle complex with tip / Type: COMPLEX / Entity ID: all / Source: NATURAL
Source (natural)Organism: Salmonella enterica subsp. enterica serovar Typhimurium (bacteria)
Strain: SL1344
Buffer solutionpH: 7.5
Buffer component
IDConc.NameFormulaBuffer-ID
1150 mMsodium chlorideNaCl1
220 mMTris HydrochlorideTris-HCl1
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportDetails: 25 mAmp / Grid material: COPPER / Grid mesh size: 400 divisions/in. / Grid type: Quantifoil R2/2
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 283 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Cs: 2.7 mm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN
Image recordingElectron dose: 50 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

SoftwareName: PHENIX / Version: 1.19.2_4158: / Classification: refinement
EM software
IDNameVersionCategory
2SerialEMimage acquisition
4Gctf1.06CTF correction
12RELION3classification
13RELION33D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C1 (asymmetric)
3D reconstructionResolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 27737 / Algorithm: BACK PROJECTION / Symmetry type: POINT
Atomic model buildingB value: 85 / Protocol: FLEXIBLE FIT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00320052
ELECTRON MICROSCOPYf_angle_d0.62427243
ELECTRON MICROSCOPYf_dihedral_angle_d4.3242693
ELECTRON MICROSCOPYf_chiral_restr0.043119
ELECTRON MICROSCOPYf_plane_restr0.0033524

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