登録情報 データベース : PDB / ID : 7oz1 構造の表示 ダウンロードとリンクタイトル Cryo-EM structure of ABCG1 E242Q mutant with ATP and cholesteryl hemisuccinate bound 要素Isoform 4 of ATP-binding cassette sub-family G member 1 詳細 キーワード MEMBRANE PROTEIN / cholesterol / ABC transporter / ATP binding / inward-open機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
ABC-type sterol transporter activity / glycoprotein transport / cellular response to high density lipoprotein particle stimulus / intracellular cholesterol transport / toxin transmembrane transporter activity / ABC transporters in lipid homeostasis / floppase activity / positive regulation of cholesterol biosynthetic process / phosphatidylcholine floppase activity / phospholipid homeostasis ... ABC-type sterol transporter activity / glycoprotein transport / cellular response to high density lipoprotein particle stimulus / intracellular cholesterol transport / toxin transmembrane transporter activity / ABC transporters in lipid homeostasis / floppase activity / positive regulation of cholesterol biosynthetic process / phosphatidylcholine floppase activity / phospholipid homeostasis / high-density lipoprotein particle remodeling / phospholipid efflux / reverse cholesterol transport / cholesterol transfer activity / low-density lipoprotein particle remodeling / トランスロカーゼ; 他の化合物の輸送を触媒; ヌクレオシド三リン酸の加水分解に伴う / HDL remodeling / cholesterol efflux / regulation of cholesterol metabolic process / positive regulation of amyloid-beta formation / response to lipid / cholesterol binding / negative regulation of cholesterol storage / amyloid precursor protein catabolic process / negative regulation of macrophage derived foam cell differentiation / positive regulation of cholesterol efflux / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / cholesterol metabolic process / : / cholesterol homeostasis / positive regulation of protein secretion / recycling endosome / phospholipid binding / transmembrane transport / ADP binding / endosome / protein heterodimerization activity / external side of plasma membrane / Golgi membrane / endoplasmic reticulum membrane / Golgi apparatus / protein homodimerization activity / ATP hydrolysis activity / mitochondrion / ATP binding / plasma membrane 類似検索 - 分子機能 Pigment precursor permease/Protein ATP-binding cassette sub-family G / ABC transporter family G domain / ABC-2 type transporter / ABC-2 type transporter / ABC-2 type transporter / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. ... Pigment precursor permease/Protein ATP-binding cassette sub-family G / ABC transporter family G domain / ABC-2 type transporter / ABC-2 type transporter / ABC-2 type transporter / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性 ADENOSINE-5'-TRIPHOSPHATE / 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / CHOLESTEROL HEMISUCCINATE / ATP-binding cassette sub-family G member 1 類似検索 - 構成要素生物種 Homo sapiens (ヒト)手法 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 4 Å 詳細データ登録者 Skarda, L. / Kowal, J. / Locher, K.P. 資金援助 スイス, 1件 詳細 詳細を隠す組織 認可番号 国 Swiss National Science Foundation スイス
引用ジャーナル : J Mol Biol / 年 : 2021タイトル : Structure of the Human Cholesterol Transporter ABCG1.著者 : Liga Skarda / Julia Kowal / Kaspar P Locher / 要旨 : ABCG1 is an ATP binding cassette (ABC) transporter that removes excess cholesterol from peripheral tissues. Despite its role in preventing lipid accumulation and the development of cardiovascular and ... ABCG1 is an ATP binding cassette (ABC) transporter that removes excess cholesterol from peripheral tissues. Despite its role in preventing lipid accumulation and the development of cardiovascular and metabolic disease, the mechanism underpinning ABCG1-mediated cholesterol transport is unknown. Here we report a cryo-EM structure of human ABCG1 at 4 Å resolution in an inward-open state, featuring sterol-like density in the binding cavity. Structural comparison with the multidrug transporter ABCG2 and the sterol transporter ABCG5/G8 reveals the basis of mechanistic differences and distinct substrate specificity. Benzamil and taurocholate inhibited the ATPase activity of liposome-reconstituted ABCG1, whereas the ABCG2 inhibitor Ko143 did not. Based on the structural insights into ABCG1, we propose a mechanism for ABCG1-mediated cholesterol transport. 履歴 登録 2021年6月25日 登録サイト : PDBE / 処理サイト : PDBE改定 1.0 2021年9月22日 Provider : repository / タイプ : Initial release改定 2.0 2021年9月29日 Group : Advisory / Atomic model ... Advisory / Atomic model / Data collection / Database references / Derived calculations / Non-polymer description / Structure summary カテゴリ : atom_site / chem_comp ... atom_site / chem_comp / citation / em_admin / entity / pdbx_database_proc / pdbx_entity_nonpoly / pdbx_unobs_or_zero_occ_atoms Item : _atom_site.Cartn_x / _atom_site.Cartn_y ... _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_atom_id / _atom_site.label_atom_id / _atom_site.pdbx_auth_atom_name / _atom_site.type_symbol / _chem_comp.formula / _chem_comp.formula_weight / _chem_comp.mon_nstd_flag / _chem_comp.name / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _em_admin.last_update / _entity.formula_weight / _entity.pdbx_description / _pdbx_entity_nonpoly.name / _pdbx_unobs_or_zero_occ_atoms.auth_atom_id / _pdbx_unobs_or_zero_occ_atoms.label_atom_id