A: ATP-binding cassette sub-family G member 5 B: ATP-binding cassette sub-family G member 8 D: ATP-binding cassette sub-family G member 8 C: ATP-binding cassette sub-family G member 5
Mass: 76985.844 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ABCG8 / Production host: Komagataella pastoris (fungus) / References: UniProt: Q9H221
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 4.08 Å3/Da / Density % sol: 69.83 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: Bicelle, ammonium sulfate, PEG 400, MES, TCEP
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Data collection
Diffraction
ID
Mean temperature (K)
Crystal-ID
1
80
1
2
80
1
Diffraction source
Source
Site
Beamline
ID
Wavelength (Å)
SYNCHROTRON
APS
19-ID
1
1
SYNCHROTRON
APS
23-ID-D
2
1
Detector
Type
ID
Detector
Date
ADSC QUANTUM 315r
1
CCD
Nov 26, 2013
MARMOSAIC 300 mm CCD
2
CCD
Oct 18, 2013
Radiation
ID
Protocol
Monochromatic (M) / Laue (L)
Scattering type
Wavelength-ID
1
SINGLEWAVELENGTH
M
x-ray
1
2
SINGLEWAVELENGTH
M
x-ray
2
Radiation wavelength
ID
Wavelength (Å)
Relative weight
1
1
1
2
1
Reflection
Resolution: 3.9→50 Å / Num. obs: 46342 / % possible obs: 99.4 % / Redundancy: 18.9 % / Net I/σ(I): 8.8
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0049
refinement
HKL-3000
datareduction
HKL-3000
datascaling
MLPHARE
phasing
Refinement
Method to determine structure: SAD / Resolution: 3.93→25 Å / Cor.coef. Fo:Fc: 0.851 / Cor.coef. Fo:Fc free: 0.731 / SU B: 65.732 / SU ML: 0.853 / Cross valid method: THROUGHOUT / ESU R Free: 1.011 Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES Details: SAD with tungstate for initial phase determination. HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.32871
1845
5 %
RANDOM
Rwork
0.24532
-
-
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obs
0.24951
34889
82.47 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK