- PDB-7lr0: Structure of squirrel TRPV1 in complex with capsaicin -
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基本情報
登録情報
データベース: PDB / ID: 7lr0
タイトル
Structure of squirrel TRPV1 in complex with capsaicin
要素
Osm-9-like TRP channel 1
キーワード
MEMBRANE PROTEIN / Transient Receptor Potential V Family Member 1 / TRP channel / TRPV1 full length / TRPV1 wild type / capsaicin
機能・相同性
機能・相同性情報
temperature-gated ion channel activity / response to capsazepine / sensory perception of mechanical stimulus / peptide secretion / detection of chemical stimulus involved in sensory perception of pain / smooth muscle contraction involved in micturition / cellular response to acidic pH / fever generation / detection of temperature stimulus involved in thermoception / dendritic spine membrane ...temperature-gated ion channel activity / response to capsazepine / sensory perception of mechanical stimulus / peptide secretion / detection of chemical stimulus involved in sensory perception of pain / smooth muscle contraction involved in micturition / cellular response to acidic pH / fever generation / detection of temperature stimulus involved in thermoception / dendritic spine membrane / calcium ion import across plasma membrane / diet induced thermogenesis / behavioral response to pain / detection of temperature stimulus involved in sensory perception of pain / extracellular ligand-gated monoatomic ion channel activity / phosphoprotein binding / calcium channel activity / lipid metabolic process / cellular response to heat / postsynaptic membrane / calmodulin binding / negative regulation of transcription by RNA polymerase II / ATP binding / metal ion binding 類似検索 - 分子機能
Transient receptor potential cation channel subfamily V member 1-4 / Transient receptor potential cation channel subfamily V / Ankyrin repeat / Ankyrin repeats (3 copies) / Ankyrin repeat profile. / Ankyrin repeat region circular profile. / ankyrin repeats / Ankyrin repeat / Ankyrin repeat-containing domain superfamily / Ion transport domain / Ion transport protein 類似検索 - ドメイン・相同性
Chem-4DY / Chem-POV / Transient receptor potential cation channel subfamily V member 1 類似検索 - 構成要素
National Institutes of Health/National Cancer Institute (NIH/NCI)
R01 CA206573
米国
National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)
R01 NS083660
米国
National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)
R01 NS107253
米国
National Science Foundation (NSF, United States)
1818086
米国
引用
ジャーナル: Nat Commun / 年: 2021 タイトル: Extracellular cap domain is an essential component of the TRPV1 gating mechanism. 著者: Kirill D Nadezhdin / Arthur Neuberger / Yury A Nikolaev / Lyle A Murphy / Elena O Gracheva / Sviatoslav N Bagriantsev / Alexander I Sobolevsky / 要旨: Transient receptor potential (TRP) channels are polymodal molecular sensors involved in numerous physiological processes and implicated in a variety of human diseases. Several structures of the ...Transient receptor potential (TRP) channels are polymodal molecular sensors involved in numerous physiological processes and implicated in a variety of human diseases. Several structures of the founding member of the TRP channel family, TRPV1, are available, all of which were determined for the protein missing the N- and C-termini and the extracellular S5-P-loop. Here, we present structures of the full-length thirteen-lined ground squirrel TRPV1 solved by cryo-EM. Our structures resolve the extracellular cap domain formed by the S5-P-loops and the C-terminus that wraps around the three-stranded β-sheet connecting elements of the TRPV1 intracellular skirt. The cap domain forms a dome above the pore's extracellular entrance, with four portals leading to the ion conductance pathway. Deletion of the cap increases the TRPV1 average conductance, reduces the open probability and affects ion selectivity. Our data show that both the termini and the cap domain are critical determinants of TRPV1 function.