+Open data
-Basic information
Entry | Database: PDB / ID: 7dst | ||||||
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Title | FMDV capsid in complex with M170 Nab | ||||||
Components |
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Keywords | VIRUS / FMDV capsid in complex with M170 Nab | ||||||
Biological species | Lama glama (llama) Foot-and-mouth disease virus | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.1 Å | ||||||
Authors | Dong, H. / Liu, P. | ||||||
Funding support | China, 1items
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Citation | Journal: Protein Cell / Year: 2022 Title: Structural and molecular basis for foot-and-mouth disease virus neutralization by two potent protective antibodies. Authors: Hu Dong / Pan Liu / Manyuan Bai / Kang Wang / Rui Feng / Dandan Zhu / Yao Sun / Suyu Mu / Haozhou Li / Michiel Harmsen / Shiqi Sun / Xiangxi Wang / Huichen Guo / | ||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7dst.cif.gz | 156.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7dst.ent.gz | 126.9 KB | Display | PDB format |
PDBx/mmJSON format | 7dst.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7dst_validation.pdf.gz | 990.5 KB | Display | wwPDB validaton report |
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Full document | 7dst_full_validation.pdf.gz | 998.3 KB | Display | |
Data in XML | 7dst_validation.xml.gz | 28.2 KB | Display | |
Data in CIF | 7dst_validation.cif.gz | 41.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ds/7dst ftp://data.pdbj.org/pub/pdb/validation_reports/ds/7dst | HTTPS FTP |
-Related structure data
Related structure data | 30845MC 7dssC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Symmetry | Point symmetry: (Schoenflies symbol: I (icosahedral)) |
-Components
#1: Protein | Mass: 22849.139 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Foot-and-mouth disease virus |
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#2: Protein | Mass: 23091.078 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Foot-and-mouth disease virus |
#3: Protein | Mass: 23754.705 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Foot-and-mouth disease virus |
#4: Protein | Mass: 7963.328 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Foot-and-mouth disease virus |
#5: Antibody | Mass: 12939.211 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Lama glama (llama) / Production host: Lama glama (llama) |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component |
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Source (natural) |
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Source (recombinant) | Organism: Lama glama (llama) | ||||||||||||||||||||||||
Buffer solution | pH: 7.4 | ||||||||||||||||||||||||
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | ||||||||||||||||||||||||
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: DARK FIELD |
Image recording | Electron dose: 30 e/Å2 / Film or detector model: GATAN K2 QUANTUM (4k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING ONLY |
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3D reconstruction | Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 3692 / Symmetry type: POINT |