+Open data
-Basic information
Entry | Database: PDB / ID: 7dlu | ||||||
---|---|---|---|---|---|---|---|
Title | Mechanosensitive channel MscS K180R mutant | ||||||
Components | Mechanosensitive ion channel MscS | ||||||
Keywords | MEMBRANE PROTEIN / Ion channel | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.5 Å | ||||||
Authors | Wu, J. / Ke, M. | ||||||
Citation | Journal: To Be Published Title: Mechanosensitive channel MscS K180R mutant Authors: Wu, J. / Ke, M. | ||||||
History |
|
-Structure visualization
Movie |
Movie viewer |
---|---|
Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7dlu.cif.gz | 296.2 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb7dlu.ent.gz | 246.9 KB | Display | PDB format |
PDBx/mmJSON format | 7dlu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7dlu_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 7dlu_full_validation.pdf.gz | 1.2 MB | Display | |
Data in XML | 7dlu_validation.xml.gz | 56.3 KB | Display | |
Data in CIF | 7dlu_validation.cif.gz | 74.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dl/7dlu ftp://data.pdbj.org/pub/pdb/validation_reports/dl/7dlu | HTTPS FTP |
-Related structure data
Related structure data | 30713MC M: map data used to model this data C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
|
---|---|
1 |
|
-Components
#1: Protein | Mass: 29870.738 Da / Num. of mol.: 7 / Mutation: K180R Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (bacteria) Gene: PA4394 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9HW12 |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Heptameric MscS K180R / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
---|---|
Molecular weight | Experimental value: NO |
Source (natural) | Organism: Pseudomonas aeruginosa (bacteria) |
Source (recombinant) | Organism: Escherichia coli (E. coli) |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
CTF correction | Type: NONE |
---|---|
3D reconstruction | Resolution: 2.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 158968 / Symmetry type: POINT |