+
Open data
-
Basic information
Entry | Database: EMDB / ID: EMD-30713 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Mechanosensitive channel MscS K180R mutant | |||||||||
![]() | ||||||||||
![]() |
| |||||||||
![]() | Ion channel / membrane protein | |||||||||
Function / homology | ![]() | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.5 Å | |||||||||
![]() | Wu J / Ke M | |||||||||
![]() | ![]() Title: Mechanosensitive channel MscS K180R mutant Authors: Wu J / Ke M | |||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-EMDB archive
Map data | ![]() | 49.7 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 11.8 KB 11.8 KB | Display Display | ![]() |
Images | ![]() | 107.7 KB | ||
Filedesc metadata | ![]() | 4.7 KB | ||
Others | ![]() ![]() | 48.8 MB 48.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7dluMC M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 1.087 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-Half map: #1
File | emd_30713_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-Half map: #2
File | emd_30713_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Projections & Slices |
| ||||||||||||
Density Histograms |
-
Sample components
-Entire : Heptameric MscS K180R
Entire | Name: Heptameric MscS K180R |
---|---|
Components |
|
-Supramolecule #1: Heptameric MscS K180R
Supramolecule | Name: Heptameric MscS K180R / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
---|---|
Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Mechanosensitive ion channel MscS
Macromolecule | Name: Mechanosensitive ion channel MscS / type: protein_or_peptide / ID: 1 / Number of copies: 7 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 29.870738 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MELNYDRLVQ QTESWLPIVL EYSGKVALAL LTLAIGWWLI NTLTGRVGGL LARRSVDRTL QGFVGSLVSI VLKILLVVSV ASMIGIQTT SFVAAIGAAG LAIGLALQGS LANFAGGVLI LLFRPFKVGD WIEAQGVAGT VDSILIFHTV LRSGDNKRII V PNGALSNG ...String: MELNYDRLVQ QTESWLPIVL EYSGKVALAL LTLAIGWWLI NTLTGRVGGL LARRSVDRTL QGFVGSLVSI VLKILLVVSV ASMIGIQTT SFVAAIGAAG LAIGLALQGS LANFAGGVLI LLFRPFKVGD WIEAQGVAGT VDSILIFHTV LRSGDNKRII V PNGALSNG TVTNYSAEPV RRVIFDVGID YDADLKNAQN ILLAMADDPR VLKDPAPVAV VSNLGESAIT LSLRVWVKNA DY WDVMFMF NEKARDALGK EGIGIPFPQR VVKVVQGAMA D UniProtKB: Uncharacterized protein |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.5 |
---|---|
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
-
Image processing
Startup model | Type of model: INSILICO MODEL |
---|---|
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 158968 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |