+Open data
-Basic information
Entry | Database: PDB / ID: 6z12 | ||||||||||||
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Title | Salmonella AcrB solubilised in the SMA copolymer | ||||||||||||
Components | Efflux pump membrane transporter | ||||||||||||
Keywords | MEMBRANE PROTEIN / AcrB | ||||||||||||
Function / homology | Function and homology information xenobiotic transport / efflux transmembrane transporter activity / membrane => GO:0016020 / plasma membrane Similarity search - Function | ||||||||||||
Biological species | Salmonella enterica I (bacteria) | ||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.6 Å | ||||||||||||
Authors | Muench, s.p. / Johnson, R.M. | ||||||||||||
Funding support | United Kingdom, 3items
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Citation | Journal: Microorganisms / Year: 2020 Title: Cryo-EM Structure and Molecular Dynamics Analysis of the Fluoroquinolone Resistant Mutant of the AcrB Transporter from . Authors: Rachel M Johnson / Chiara Fais / Mayuriben Parmar / Harish Cheruvara / Robert L Marshall / Sophie J Hesketh / Matthew C Feasey / Paolo Ruggerone / Attilio V Vargiu / Vincent L G Postis / ...Authors: Rachel M Johnson / Chiara Fais / Mayuriben Parmar / Harish Cheruvara / Robert L Marshall / Sophie J Hesketh / Matthew C Feasey / Paolo Ruggerone / Attilio V Vargiu / Vincent L G Postis / Stephen P Muench / Vassiliy N Bavro / Abstract: is an important genus of Gram-negative pathogens, treatment of which has become problematic due to increases in antimicrobial resistance. This is partly attributable to the overexpression of ... is an important genus of Gram-negative pathogens, treatment of which has become problematic due to increases in antimicrobial resistance. This is partly attributable to the overexpression of tripartite efflux pumps, particularly the constitutively expressed AcrAB-TolC. Despite its clinical importance, the structure of the AcrB transporter remained unknown to-date, with much of our structural understanding coming from the orthologue. Here, by taking advantage of the styrene maleic acid (SMA) technology to isolate membrane proteins with closely associated lipids, we report the very first experimental structure of AcrB transporter. Furthermore, this novel structure provides additional insight into mechanisms of drug efflux as it bears the mutation (G288D), originating from a clinical isolate of Typhimurium presenting an increased resistance to fluoroquinolones. Experimental data are complemented by state-of-the-art molecular dynamics (MD) simulations on both the wild type and G288D variant of AcrB. Together, these reveal several important differences with respect to the protein, providing insights into the role of the G288D mutation in increasing drug efflux and extending our understanding of the mechanisms underlying antibiotic resistance. | ||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 6z12.cif.gz | 932.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6z12.ent.gz | 781.1 KB | Display | PDB format |
PDBx/mmJSON format | 6z12.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6z12_validation.pdf.gz | 995.8 KB | Display | wwPDB validaton report |
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Full document | 6z12_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 6z12_validation.xml.gz | 95.2 KB | Display | |
Data in CIF | 6z12_validation.cif.gz | 143.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/z1/6z12 ftp://data.pdbj.org/pub/pdb/validation_reports/z1/6z12 | HTTPS FTP |
-Related structure data
Related structure data | 4460MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
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-Components
#1: Protein | Mass: 113759.227 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salmonella enterica I (bacteria) Gene: acrB, A4O23_10955, A4O45_03810, A5895_15265, AAC70_12525, AAL03_14960, AAM89_07765, AAV76_19425, ABK55_05725, ABO08_03475, AC391_10965, ACH21_19020, ACK40_00105, ACK79_15085, ACS18_20650, ACV85_ ...Gene: acrB, A4O23_10955, A4O45_03810, A5895_15265, AAC70_12525, AAL03_14960, AAM89_07765, AAV76_19425, ABK55_05725, ABO08_03475, AC391_10965, ACH21_19020, ACK40_00105, ACK79_15085, ACS18_20650, ACV85_05805, ADQ53_03685, ADQ64_15580, ADT85_17470, AF726_08820, AG46_12710, AG53_15950, AGQ41_04335, AGQ90_03080, AGR09_08325, AGR46_16415, AH060_16145, AH079_04350, AH254_22600, AH360_16970, AH543_16315, AH557_09130, AH612_19020, AH874_13175, AHN36_15795, AHN91_21865, AHQ94_20600, AHS94_22030, AHU36_11045, AHU48_23190, AHX56_08240, AHY46_22370, AHZ72_05105, AIA22_12165, AIB97_16815, AID13_02815, AIE18_09140, AIF85_07160, AL864_02820, AL929_23130, ALX58_14045, AU885_11650, AVA95_03925, AVB28_08510, AVG51_11480, AVH38_04545, AXK79_19705, AXM38_07800, AXM50_14470, AXM56_16685, AXM65_18530, AXM93_08445, AXN14_02155, AXN15_07580, AXN21_06965, AXN39_11550, AXN51_12700, AXN72_08000, AXO05_08570, AXO78_17315, AXO93_15340, AXQ08_14655, AXR02_08970, AXR82_02315, AXR91_01290, AXS04_01695, AXS89_02055, AXT07_05965, AXT19_05915, B9C63_11810, BXE81_09065, BXG02_07180, BXG42_09370, BXH36_10215, BXH66_08040, BXL85_11765, BXO48_06645, BZ557_12735, BZ723_04100, BZ903_08940, BZV91_08875, BZW24_03920, BZW54_04755, C9E93_06140, C9E94_19180, C9F01_08965, C9F05_01290, C9P23_10335, C9Y45_03940, CB317_01000, CC394_01545, CC530_00775, CC828_04535, CE94_19610, CF93_04280, CPY04_22475, CQE41_07070, CQF73_09855, CQH69_04135, CQM91_23105, CSM01_01615, CSN39_10900, CSN48_19880, CSN51_17860, CSO96_14135, CSP66_16145, CST74_11845, CST78_12945, CV491_04375, CWG53_02155, CWG97_11285, D4346_01325, D4392_10020, D4396_04285, D4408_06485, D4427_06950, D4428_05215, D4444_11555, D4471_08450, D4490_01015, D4494_10470, D4507_05270, D4514_03660, D4515_01545, D4546_09390, D4551_09260, D4D97_05485, D4D98_08570, D4E01_09290, D4E37_05350, D4E64_10565, D4E75_00440, D4E90_05185, D4F17_01060, D4F19_04440, D4F20_07155, D4F29_07730, D4F49_16455, D4F54_04680, D4F77_10190, D4F87_02985, D4F98_10640, D4G07_10045, D4G10_11575, D4G50_05510, D4X66_01450, D4X67_01465, D4X81_08350, D4Y13_13605, D4Y27_04430, D4Y38_00790, D4Y42_06150, D4Y56_03795, D4Y61_08865, D4Y96_04575, D4Z14_05470, D4Z16_07845, D4Z17_03400, D4Z29_11545, D4Z41_08675, D4Z50_07970, D4Z57_03410, D4Z58_04655, D4Z75_04825, D5764_07825, D5769_02495, D5773_00875, D5782_03660, D5810_01285, D5836_17200, D5848_11770, D5856_03150, D5861_06225, D5881_04345, D5889_07675, D5895_01345, D5905_18015, D5915_03200, D5970_05735, D5972_06010, D5A61_03660, D5A65_05805, D5A76_04875, D5A82_03865, D5A94_12405, D5B23_01450, D5B60_01340, D5B66_05165, D5B94_03135, D5C00_05295, D5C12_08175, D5C21_07220, D5C32_06325, D5C34_10465, D5C40_07860, D5C41_08390, D5C46_12375, D5C64_03810, D5C69_08580, D5O45_04325, D5O48_05730, D5O74_04490, D5O78_09935, D5O80_07835, D5O86_11185, D5P09_09970, D5P22_01470, D5P31_04355, D5P36_04640, D5P42_03925, D5P52_00720, D5P54_05700, D5P59_05915, D5P71_08860, D5P75_03560, D5P94_16990, D5Q05_03935, D5Q09_06085, D5Q39_11635, D5Q43_00445, D5W53_06130, D5W81_01065, D5W91_06810, D5X16_03865, D5X20_07410, D5X39_00990, D5X56_02780, D5X61_00680, D5X72_03440, D5X88_07550, D5X91_04060, D5Y01_00825, D5Y06_08270, D5Y08_23240, D5Y41_01425, D6331_01310, D6379_03305, D6389_04215, D6413_06485, D6429_04170, D6448_15635, D6453_04205, D6455_06220, D6467_08685, D6J43_21925, D6J46_01025, D6J55_02800, D6J67_02330, D6J74_05385, D6J84_08940, D6K21_03765, D6K24_01080, D6K59_04410, D6L05_07630, D6P24_03240, D6P41_01305, D6P57_11975, D6P63_03960, D6Q07_11960, D6Q21_01005, D6Q25_01020, D6Q70_23040, D6Q87_10085, D6Q96_09190, D6Q97_04185, D6R14_09640, D6R58_05020, D6R62_09555, D6R98_02810, D6S02_04705, D6S13_01360, D6S22_05290, D6S36_05340, D6S45_01045, D6S47_07470, D6S50_05845, D6S52_07325, D6S74_01450, D6S83_06345, D6S89_03890, D6T18_03025, D6T31_08100, D6T37_07430, D7M89_05935, D7M98_11725, D7N03_02455, D7N07_08155, D7N22_06745, D7N26_22710, D7N30_05075, D7N47_05845, D7N51_15930, D7N52_04690, D7N80_02885, D7N86_17235, D7O13_06330, D7O17_07925, D7O23_11190, D7O25_01030, D7O28_08970, D7O59_06225, D7O77_04615, D8Q64_01020, D8Q73_05540, D8R14_01045, D8R18_08390, D8R62_10510, D8R78_07850, D8S31_02190, D8S34_08660, D8S35_08175, D8S37_06530, D8S38_08610, D8S62_04305, D8S66_05780, D9O60_02875, D9O71_05360, D9O80_03040, D9O86_13290, D9O95_03895, D9P04_07965, D9P12_05975, D9P13_14425, D9P19_07675, D9P28_09530, D9P30_03370, D9P43_10815, D9P51_02955, D9P55_07730, D9P58_06870, D9P68_09885, D9P88_00985, D9P92_02905, D9P97_06685, D9Q07_09260, D9Q11_05725, D9Q23_03710, D9Q29_06205, D9Q37_07930, D9Q49_08285, D9Q50_10325, DAR35_01005, DAW17_06730, DAX91_07060, DFQ85_05430, DFR01_09160, DK013_01025, DK030_06300, DK052_23015, DK083_04615, DK093_01040, DK094_01025, DK101_08130, DK113_08305, DK117_07485, DK127_09260, DK678_00070, DK681_09420, DK687_04055, DK711_01855, DK719_05700, DK739_06740, DK741_10685, DK756_03460, DK767_12285, DKI70_07030, DKJ03_09570, DKJ07_05835, DKS84_08230, DKT00_02710, DKT04_15920, DKT21_12805, DKT27_10265, DKT52_07220, DKU65_14915, DKV05_06000, DKV20_06200, DKV25_04445, DL088_11075, DL104_17055, DL110_05250, DL111_06640, DL115_01355, DL130_03140, DL146_02805, DLB02_07650, DLB18_08505, DLB23_01000, DLB36_10070, DLB43_07510, DLB46_07880, DLB62_03890, DLB73_00105, DLC24_04935, DLE90_18415, DLF22_04805, DLF29_05020, DLF30_08180, DLF33_10805, DLF35_01005, DLF36_02845, DLM06_04560, DLM09_00995, DLM10_09965, DLM30_04930, DLQ73_12300, DLQ79_01000, DLQ86_15735, DLQ92_03590, DLR05_06915, DLR06_01490, DLR25_05290, DLR57_10745, DM001_07440, DM006_09100, DM016_05255, DM323_06700, DM347_08405, DM358_08140, DM367_10780, DM371_02430, DM700_18145, DM703_02460, DM705_08310, DM743_09605, DMA85_03170, DMA89_15850, DMB03_08830, DMB04_15185, DML85_00605, DML97_09050, DMM16_01960, DMM17_11150, DMM22_02845, DMM24_11405, DMM43_07890, DMO61_03735, DMO65_01050, DMO79_09060, DMU74_10415, DMU80_09050, DMU82_12600, DMU94_09600, DMV06_03650, DMV13_03350, DMV20_20060, DMV32_00825, DMV34_02950, DMZ76_01020, DN020_06485, DN087_03575, DN092_04545, DN123_07690, DN125_01440, DN130_05830, DN132_04380, DN181_04005, DN182_06745, DN220_06100, DN241_03340, DN257_05145, DN259_05090, DN271_19320, DN282_15665, DN317_01415, DN319_10795, DN322_05135, DN358_10690, DN362_06885, DNI00_08045, DNI25_05135, DNI46_06020, DNI61_09285, DNI77_05260, DNI82_12125, DNI85_03675, DNJ05_06165, DNJ30_02305, DNL79_03545, DNM18_05050, DNM22_08605, DNM39_12310, DNM44_03995, DNM47_08685, DNM48_03895, DNM80_06910, DNN00_05420, DNU17_09140, DNU22_04660, DNU30_01285, DNU40_06770, DNU46_14660, DNU67_08335, DNU68_11160, DNU78_13165, DNU82_03725, DNU84_13710, DNU93_05320, DNV11_03960, DNV18_07230, DNV24_12015, DNV55_05965, DNV93_02810, DNW15_00865, DNY10_04300, DNY38_00420, DNY86_09760, DNZ14_01330, DNZ24_04550, DNZ41_01315, DNZ69_08840, DNZ74_07090, DNZ75_08705, DNZ81_03425, DNZ91_05745, DO557_05890, DO565_08085, DO591_06685, DO596_04935, DO617_04810, DO619_02490, DO630_04000, DO638_05650, DO649_13400, DO654_06280, DO661_06270, DO663_04365, DO672_01035, DO679_08555, DO681_04055, DO690_00395, DO696_07715, DO712_22550, DO727_09960, DO731_01530, DO771_06730, DO807_07800, DO827_06795, DO973_04300, DO981_01690, DO994_01335, DOA98_08020, DOB00_09740, DOB23_01440, DOB53_16610, DOB60_05740, DOB76_01635, DOC12_00300, DOC15_05765, DOC25_09930, DOC31_08415, DOC34_09315, DOC40_14960, DOC63_04280, DOG94_02935, DOH04_12155, DOH12_22545, DOH33_03235, DOH41_03550, DOH63_02830, DOH69_10710, DOH74_13625, DOH75_06000, DOH81_09930, DOI30_01020, DOI60_10480, DOI66_05845, DOJ02_05395, DOQ45_03150, DOQ73_06415, DOQ79_01065, DOR42_03375, DOR43_04775, DOR46_16150, DOR51_23780, DOR52_02535, DOR63_07395, DOR64_10515, DOR75_06845, DOV68_03030, DOV71_04785, DOV77_15350, DOV89_01625, DOW27_10165, DOW52_22585, DOW74_04075, DOX21_02055, DOX30_03420, DP688_13895, DP693_00825, DP698_06830, DP703_05145, DP712_02970, DP713_03380, DP716_06840, DP727_22840, DP728_16275, DP736_07365, DP748_02490, DP766_03155, DP778_03250, DP792_05710, DP800_16220, DP812_13225, DP837_03195, DP859_02865, DP873_09820, DP884_06665, DPA01_04065, DPA17_01430, DPA21_08565, DPA36_04755, DPA81_08695, DPA91_07750, DPB01_05745, DPB58_05530, DPB61_01065, DPB73_06705, DPB80_01010, DPC17_04415, DPC26_12120, DPC30_09225, DPC31_21295, DPC91_07340, DPD28_03410, DPD35_09350, DPD61_05565, DPD71_12990, DPD96_04945, DPE17_03960, DPE32_06850, DPE40_05345, DPE61_01100, DPE66_10470, DPE72_00995, DPE76_10585, DPE91_07335, DPE98_01700, DPF18_01415, DPF55_03280, DPF57_13210, DPF65_05290, DPF69_04225, DPJ06_06690, DPJ07_04770, DPJ18_21730, DPJ21_23060, DPJ65_05985, DPJ69_12010, DPJ81_01030, DPK19_02945, DPK49_18945, DPL03_00940, DPP06_22450, DPP34_02995, DPP49_05140, DPP57_09185, DPP70_04625, DPR96_17095, DPS51_01190, DPS62_02400, DPS71_04035, DPS75_05935, DPS77_06815, DPS96_17080, DPT22_03505, DPT41_07335, DPT74_05620, DPT84_17600, DPT95_03965, DPU11_05080, DPU13_04230, DPY66_15080, DPY81_09375, DPZ06_03150, DPZ23_03295, DPZ65_01920, DPZ67_03455, DPZ88_09965, DPZ95_04280, DQ003_10345, DQ017_21125, DQ037_03770, DQ055_06250, DQ072_13750, DQ076_16795, DQ078_07335, DQ082_06505, DQ790_02530, DQ802_06935, DQ816_09465, DQ836_21070, DQ837_05050, DQ839_09850, DQ845_10875, DQ858_00055, DQ871_15205, DQ880_07325, DQ895_06115, DQ901_09380, DQ919_02340, DQ933_07675, DQ978_14715, DQ985_10135, DQC12_07750, DQC17_05135, DQC26_03870, DQC75_03590, DQC77_06245, DQC81_16925, DQC82_09655, DQC88_01435, DQD09_24240, DQD11_01060, DQD13_03865, DQD28_13095, DQD46_02455, DQD59_03945, DQE08_00395, DQE20_03160, DQE31_05590, DQE33_13015, DQE40_13690, DQE42_08765, DQJ22_01340, DQJ50_08905, DQJ58_03830, DQJ63_05540, DQJ66_02440, DQJ71_08430, DQJ77_01030, DQJ83_05310, DQJ90_08450, DQK19_10845, DQK43_01440, DQK45_13610, DQK53_05425, DQK57_20355, DQK72_09740, DQQ27_15440, DQR39_03355, DQR40_15910, DQR74_07115, DQR85_07280, DQR90_05875, DQS34_02500, DQS55_04230, DQS56_07240, DQS62_04550, DQS71_07420, DQS74_06295, DQS89_04330, DQS98_04035, DQT21_23885, DQT31_01485, DQT40_01975, DQT47_08080, DQT59_08365, DQT67_04950, DQT85_05480, DQY62_10275, DQY86_01365, DQY91_07170, DQZ13_08220, DQZ68_04575, DQZ89_03655, DRA21_00825, DRA23_02570, DRA25_06475, DRA26_15005, DRA30_07960, DRA37_10395, DRD38_06525, DRD40_04685, DRD55_03550, DRD57_02825, DRD79_08175, DRE09_03295, DRE12_04035, DRE16_07695, DRE32_01375, DRE41_16345, DRE46_06240, DRE58_03660, DRE69_05135, DRE85_05775, DRE92_01430, DRF10_01070, DRF26_07295, DRF38_06650, DRK15_08305, DRK36_10935, DRK49_10210, DRK71_14235, DRK91_06225, DRL06_03150, DRL40_04985, DRL53_01550, DRL62_15150, DRL63_08155, DRL65_05050, DRL68_04235, DRL89_01035, DRL96_01185, DRM19_02850, DRM39_10070, DRM41_05515, DRM43_02825, DRM75_09425, DRT10_03415, DRT15_04045, DRT45_11245, DRT49_10675, DRT56_11825, DRT73_05670, DRT76_09345, DRT77_01030, DRU07_04960, DRU14_01025, DRU25_12475, DRU99_06930, DRV08_07615, DRV17_01045, DRV44_09955, DRV53_06735, DRV60_09675, DRV62_04555, DRW94_06120, DRX19_09970, DRX40_11405, DRX43_00440, DRY30_09095, DRY35_08945, DRY53_03870, DRY54_04540, DRY73_06120, DRZ18_11450, DS161_03605, DS168_01430, DS172_06735, DS181_05705, DS188_01805, DS209_03915, DS212_06790, DS228_01025, DS242_04605, DS251_06835, DS266_01340, DS269_03610, DS300_04175, DS306_05655, DS313_01340, DS324_09265, DS336_04600, DS340_09465, DS346_12760, DS368_20110, DS372_11155, DS390_10475, DS438_03885, DS468_03185, DS470_17635, DS485_05030, DS497_03915, DS570_05955, DS572_04860, DS605_10670, DS615_10365, DS651_15910, DS657_02820, DS688_11950, DS689_13215, DS708_08160, DSA82_08035, DSA90_01010, DSA91_03875, DSA99_10695, DSB20_06505, DSB26_11025, DSB29_01055, DSB36_03930, DSB44_04370, DSE94_03270, DSF03_05385, DSF06_15125, DSF13_00100, DSF32_06125, DSF57_04690, DSF62_14785, DSF92_08520, DSF93_06370, DSG01_03865, DSG07_07830, DSG23_03190, DSG69_22660, DSG96_09425, DSH12_03820, DSM73_23085, DSM81_02825, DSM84_01405, DSM85_11930, DSM96_17705, DSN05_00365, DSN18_08025, DSN41_12095, DSQ65_01425, DSQ94_01375, DSR19_03505, DSR78_10785, DSR82_03690, DSR83_08440, DSR88_10410, DSS12_01435, DSS23_05770, DSS37_03960, DSS38_06180, DSS62_22355, DSS75_06400, DSS77_03870, DST00_18010, DST18_17355, DT136_10790, DT138_00540, DT142_21420, DT150_04765, DT159_10485, DT168_04075, DT188_03155, DT193_04870, DT208_00940, DT222_17390, DTE69_04010, DTE86_06360, DTF17_05870, DTF33_02970, DTF39_03290, DTF59_06550, DTF80_05780, DTF85_01005, DTF97_01065, DTG02_02595, DTG11_04415, DTG12_07185, DTG17_13435, DTG24_05555, DTG32_01040, DTG45_10785, DTG47_08540, DTG49_04600, DTG64_04755, DTG68_11905, DTG84_10305, DTH05_03950, DTH08_07170, DTH13_09220, DTT89_02880, DTT93_01025, DTT96_06630, DTT99_02425, DTU24_08720, DTU25_07215, DTU37_09945, DTU48_07545, DTU57_10990, DTU59_09415, DTU60_01035, DTU87_17590, DTV05_07485, DTV16_05625, DTV63_11510, DTV88_17040, DTV89_05155, DTW09_15585, DTW19_02845, DU084_01010, DU092_06545, DU116_00420, DU129_23205, DU131_04030, DU148_01355, DU157_05050, DU159_12015, DU161_00750, DU165_03845, DU168_05890, DU203_03365, DU210_17755, DU215_13830, DU245_04670, DU257_01045, DU748_01890, DU754_02765, DU756_10340, DU758_06015, DU761_12840, DU762_05800, DU777_01410, DU806_14595, DU811_19780, DU834_07665, DU842_16540, DU844_07525, DU852_09995, DU854_02500, DU861_06795, DU873_02415, DU877_10080, DU916_06380, DU946_01030, DU947_01060, DU949_04885, DU954_06100, DUA40_08690, DUA46_06400, DUA52_07680, DUA53_05475, DUA65_00100, DUA91_01005, DUA93_13100, DUB05_08355, DUB53_09680, DUB79_09560, DUB80_04325, DUB82_06390, DUC24_06685, DUC35_07030, DUE02_23055, DUP59_09460, DUP60_01040, DUP67_00775, DUP70_02675, DUP71_10245, DUP74_21535, DUP91_09330, DUQ24_04285, DUQ35_01340, DUQ37_21005, DUQ39_06135, DUQ41_06535, DUQ55_11365, DUQ58_03480, DUQ62_06195, DUQ64_00590, DUQ71_20825, DUQ75_09255, DUQ82_05805, DUQ83_02605, DUQ85_06520, DUR69_18605, DUR85_01530, DUR88_02530, DUR90_07210, DUR96_16820, DUU13_17140, DUU22_03635, DUU35_10475, DUU38_03210, DUU43_07880, DUU44_03135, DUU47_14840, DUU54_17805, DUU71_16085, DUU78_10680, DUU80_06735, DUU92_05345, DUV18_06490, DUV49_17005, DUV51_09990, DUV63_07195, DUV70_07150, DUV72_12440, DUV91_06335, DUW11_05640, DUW53_01400, DV01_10115, DVE93_04900, DVF01_04210, DVF33_03245, DVF55_03535, DVF58_17950, DVG00_04280, DVG02_02975, DVG21_09825, DVG27_01320, DVG41_07205, DVG59_06390, E0U76_06940, E1723_08390, E5N87_02445, EBB74_08700, EBB77_10485, EBB78_09315, EBB82_05950, EBB89_03075, EBC18_06325, EBC34_05750, EBC39_09715, EBC44_20925, EBC68_09380, EBD00_08225, EBD05_16450, EBD20_17095, EBD25_08895, EBD36_08845, EBD43_05210, EBD77_10535, EBJ70_10680, EBJ71_01010, EBJ89_06925, EBJ93_03490, EBJ94_10965, EBK05_09080, EBK23_03750, EBK37_10015, EBK43_02755, EBK60_12435, EBK61_03755, EBO78_16280, EBO89_05830, EBO94_06365, EBP03_08425, EBP04_03395, EBP12_01030, EBP28_05175, EC417_00970, EC420_04945, EC422_04495, EC429_01340, EC434_00995, EC448_08925, EC453_08560, EC471_06365, EC474_02880, EC491_11165, EC492_01205, EC499_09710, ECA60_08605, ECA66_03335, ECA89_08860, ECA94_02770, ECA96_08230, ECB03_04845, ECB08_02380, ECC57_03340, ECC66_14845, ECD11_05680, ECD14_09420, ECD30_08195, ED432_06430, ED436_04010, ED469_03260, ED477_03225, ED486_04135, EDK87_02770, EDL23_06330, EDL36_02800, EDL46_04335, EEA55_06135, EEA59_06700, EEA63_06475, EEA67_03960, EEA91_03575, EEA93_12055, EER52_06640, EER72_05775, EER74_06695, EER75_01000, EER80_15065, EES07_03330, EES08_04495, EES26_06675, EF649_00790, EF653_07695, EF657_16060, EF664_03080, EF665_06445, EF675_05800, EF704_09510, EFQ12_01835, EFQ27_03795, EFQ33_05115, EFQ52_05980, EFQ76_04175, EGL07_15310, EGL44_08725, EGL51_05850, EGL56_03560, EGM22_05950, EGM25_01025, EGM30_19890, EH095_03075, EH102_06310, EH104_01460, EH113_07645, EH130_06335, EHB16_03305, EHB26_04220, EHB38_03060, EHB40_08960, EHB51_06420, EHB53_05245, EHB61_03800, EHB67_09945, EHB73_13145, EHC02_01270, EHC04_15005, EHD17_01205, EHD20_04800, EHD23_03730, EHE53_08440, EHE71_11715, EHE79_04055, EHE81_05630, EHF16_05060, EIE05_03115, EIE15_00620, EIE18_03485, EIE24_02515, EIE42_04075, EIE60_01415, EIK95_05630, EIK98_05950, EIL32_01395, EIW55_09875, EIW58_12505, EIW69_03350, EIW86_10825, EIW93_08525, EK92_17045, ELE06_11115, EUC09_08855, EWC36_09200, EWD86_04885, EXB88_06730, EZX96_06900, F0D09_08975, F0D12_09400, F0D14_05560, F0D29_07175, F0D58_06620, F0H97_08960, F0I33_07995, F0M26_03705, F0M28_05115, F1011_12850, F1J30_00530, F1J33_06075, F1J42_08565, F1J81_05590, F1J82_05610, F1J83_01475, F1J85_04315, F1J88_01420, F1J90_05745, F1K42_02430, F1K59_05050, F2J39_01835, F2J40_00980, F2J48_00940, F2J50_07090, F2J62_03650, F2J66_01280, F2J78_05500, F2K19_06960, F2K22_06105, F2K26_14940, F2V07_07055, F2V23_03890, F2V25_06240, F3550_01355, F3552_00850, F3554_06545, F3591_11485, F3595_10525, F3603_03295, F3626_14980, F3E16_02765, F3E20_04290, F3E26_03740, F3E44_09410, F3E63_09210, F3E73_03605, F3E77_03585, F3E81_09345, F3P41_00915, F3P70_08045, F3P71_01925, F3P74_06490, F3P75_12190, F3P83_04915, F3Q00_03840, F3Q03_05030, F3Q14_02890, F3Q15_02920, F3Q17_04825, F3Q19_00915, F3Q28_06760, F3Q63_04915, F3R19_08235, F3X32_06195, F3X39_18195, F3X53_04150, F3X54_06825, F3X74_09220, F6430_00280, F7247_03195, F7801_03470, F9P04_11415, F9P26_00380, F9Q85_11085, F9W02_05115, F9X76_16290, F9X98_08000, FE718_01360, FE738_01015, FE759_06665, FKN96_12870, FL809_10360, FMV10_12745, FP220_08405, FPF85_03010, FQS21_06835, FQS30_06725, FQS47_00615, FQS59_09325, FR245_13140, FR247_08285, FRM70_05500, FRM79_14805, FRN01_11890, FRN02_05030, FRN09_04175, FRN22_13530, FY061_02260, FZ484_04550, FZ491_03810, FZ502_10420, FZ514_03745, FZ515_13330, FZ516_05975, FZ523_01470, FZ527_01765, FZ528_04325, FZT79_05320, GAW88_10720, GCH48_00965, GCX84_03060, GCY28_22200, GCY29_11985, GDH50_05360, GDH65_00960, GN92_19330, GX90_25025, JF03_19690, JR37_17475, KO25_20305, LB54_08610, LC09_03640, LC85_05730, LH24_08730, LH82_14165, LP13_20040, MB64_17020, NB08_11105, NCTC8272_02769, NU30_22415, PK04_18245, PK95_13970, PV65_06390, QC88_19815, QD42_08685, QD82_16765, QE89_08685, QE93_10380, QF21_24215, R118_18445, R126_08305, R133_19845, RU16_07410, RU46_12145, RY46_16045, RY52_16035, S021_09745, S313_01135, S315_02435, S598_12875, S639_21410, S709_07710, S764_16455, S943_05250, TE27_11430, TL61_17385, VT29_18010, WB97_02550, XJ41_07365, XK54_01525, XK85_16820, XS28_05335, XT48_02390, XZ16_15885, Y847_07225, YA74_10200, YH39_16290, YI72_11450, YJ27_07680, YL04_09955, YL55_19460, YR14_06075, YU10_04620, Z155_07555, Z599_02180, ZA28_00110, ZA51_00590, ZA74_09315, ZD91_01440, ZF07_21535, ZG82_06210, ZO67_01860 Production host: Escherichia coli (E. coli) / References: UniProt: A0A3U3J7F4, UniProt: Q8ZRA7*PLUS |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: AcrB / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: Salmonella enterica subsp. enterica serovar Typhi (bacteria) |
Source (recombinant) | Organism: Escherichia coli (E. coli) |
Buffer solution | pH: 7.5 |
Specimen | Conc.: 1 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 60 e/Å2 / Film or detector model: FEI FALCON III (4k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.12_2829: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
Symmetry | Point symmetry: C3 (3 fold cyclic) | ||||||||||||||||||||||||
3D reconstruction | Resolution: 4.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 316000 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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