Putative type IV secretory system protein / Type IV secretory system, conjugal DNA-protein transfer / DotD protein / DotD superfamily / DotD protein / Putative outer membrane core complex of type IVb secretion / Putative outer membrane core complex of type IVb secretion / : / OmpA-like domain superfamily / OmpA family ...Putative type IV secretory system protein / Type IV secretory system, conjugal DNA-protein transfer / DotD protein / DotD superfamily / DotD protein / Putative outer membrane core complex of type IVb secretion / Putative outer membrane core complex of type IVb secretion / : / OmpA-like domain superfamily / OmpA family / OmpA-like domain / OmpA-like domain profile. / Prokaryotic membrane lipoprotein lipid attachment site profile. 類似検索 - ドメイン・相同性
Type IV secretion protein IcmK / DotD / DotC / Inner membrane lipoprotein YiaD / Outer membrane protein, OmpA family protein 類似検索 - 構成要素
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI150027-01
米国
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)
2T32DK007673
米国
National Institutes of Health/Office of the Director
S10OD020011
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
U24 GM129539
米国
Other private
SF349247
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
5R01AI095755-10
米国
Other government
1I01BX004447
米国
引用
ジャーナル: Elife / 年: 2020 タイトル: Structural analysis of the Dot/Icm type IV secretion system core complex. 著者: Clarissa L Durie / Michael J Sheedlo / Jeong Min Chung / Brenda G Byrne / Min Su / Thomas Knight / Michele Swanson / D Borden Lacy / Melanie D Ohi / 要旨: is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires' Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver ... is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires' Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver effector proteins into host cells. Compared to prototypical T4SSs, the Dot/Icm assembly is much larger, containing ~27 different components including a core complex reported to be composed of five proteins: DotC, DotD, DotF, DotG, and DotH. Using single particle cryo-electron microscopy (cryo-EM), we report reconstructions of the core complex of the Dot/Icm T4SS that includes a symmetry mismatch between distinct structural features of the outer membrane cap (OMC) and periplasmic ring (PR). We present models of known core complex proteins, DotC, DotD, and DotH, and two structurally similar proteins within the core complex, DotK and Lpg0657. This analysis reveals the stoichiometry and contact interfaces between the key proteins of the Dot/Icm T4SS core complex and provides a framework for understanding a complex molecular machine.
AC: DotC AD: DotD AH: Type IV secretion protein IcmK AK: Inner membrane lipoprotein YiaD AX: Type IV secretion system unknown protein fragment AY: Type IV secretion system unknown protein fragment AZ: Type IV secretion system unknown protein fragment Ad: DotD BC: DotC BD: DotD BH: Type IV secretion protein IcmK BK: Inner membrane lipoprotein YiaD BX: Type IV secretion system unknown protein fragment BY: Type IV secretion system unknown protein fragment BZ: Type IV secretion system unknown protein fragment Bd: DotD CC: DotC CD: DotD CH: Type IV secretion protein IcmK CK: Inner membrane lipoprotein YiaD CX: Type IV secretion system unknown protein fragment CY: Type IV secretion system unknown protein fragment CZ: Type IV secretion system unknown protein fragment Cd: DotD DC: DotC DD: DotD DH: Type IV secretion protein IcmK DK: Inner membrane lipoprotein YiaD DX: Type IV secretion system unknown protein fragment DY: Type IV secretion system unknown protein fragment DZ: Type IV secretion system unknown protein fragment Dd: DotD EC: DotC ED: DotD EH: Type IV secretion protein IcmK EK: Inner membrane lipoprotein YiaD EX: Type IV secretion system unknown protein fragment EY: Type IV secretion system unknown protein fragment EZ: Type IV secretion system unknown protein fragment Ed: DotD FC: DotC FD: DotD FH: Type IV secretion protein IcmK FK: Inner membrane lipoprotein YiaD FX: Type IV secretion system unknown protein fragment FY: Type IV secretion system unknown protein fragment FZ: Type IV secretion system unknown protein fragment Fd: DotD GC: DotC GD: DotD GH: Type IV secretion protein IcmK GK: Inner membrane lipoprotein YiaD GX: Type IV secretion system unknown protein fragment GY: Type IV secretion system unknown protein fragment GZ: Type IV secretion system unknown protein fragment Gd: DotD HC: DotC HD: DotD HH: Type IV secretion protein IcmK HK: Inner membrane lipoprotein YiaD HX: Type IV secretion system unknown protein fragment HY: Type IV secretion system unknown protein fragment HZ: Type IV secretion system unknown protein fragment Hd: DotD IC: DotC ID: DotD IH: Type IV secretion protein IcmK IK: Inner membrane lipoprotein YiaD IX: Type IV secretion system unknown protein fragment IY: Type IV secretion system unknown protein fragment IZ: Type IV secretion system unknown protein fragment Id: DotD JC: DotC JD: DotD JH: Type IV secretion protein IcmK JK: Inner membrane lipoprotein YiaD JX: Type IV secretion system unknown protein fragment JY: Type IV secretion system unknown protein fragment JZ: Type IV secretion system unknown protein fragment Jd: DotD KC: DotC KD: DotD KH: Type IV secretion protein IcmK KK: Inner membrane lipoprotein YiaD KX: Type IV secretion system unknown protein fragment KY: Type IV secretion system unknown protein fragment KZ: Type IV secretion system unknown protein fragment Kd: DotD LC: DotC LD: DotD LH: Type IV secretion protein IcmK LK: Inner membrane lipoprotein YiaD LX: Type IV secretion system unknown protein fragment LY: Type IV secretion system unknown protein fragment LZ: Type IV secretion system unknown protein fragment Ld: DotD MC: DotC MD: DotD MH: Type IV secretion protein IcmK MK: Inner membrane lipoprotein YiaD MX: Type IV secretion system unknown protein fragment MY: Type IV secretion system unknown protein fragment MZ: Type IV secretion system unknown protein fragment Md: DotD AA: Outer membrane protein, OmpA family protein BA: Outer membrane protein, OmpA family protein CA: Outer membrane protein, OmpA family protein DA: Outer membrane protein, OmpA family protein EA: Outer membrane protein, OmpA family protein FA: Outer membrane protein, OmpA family protein GA: Outer membrane protein, OmpA family protein HA: Outer membrane protein, OmpA family protein IA: Outer membrane protein, OmpA family protein JA: Outer membrane protein, OmpA family protein KA: Outer membrane protein, OmpA family protein LA: Outer membrane protein, OmpA family protein QA: Outer membrane protein, OmpA family protein