ジャーナル: J Mol Biol / 年: 2016 タイトル: Structure of AP205 Coat Protein Reveals Circular Permutation in ssRNA Bacteriophages. 著者: Mihails Shishovs / Janis Rumnieks / Christoph Diebolder / Kristaps Jaudzems / Loren B Andreas / Jan Stanek / Andris Kazaks / Svetlana Kotelovica / Inara Akopjana / Guido Pintacuda / Roman I ...著者: Mihails Shishovs / Janis Rumnieks / Christoph Diebolder / Kristaps Jaudzems / Loren B Andreas / Jan Stanek / Andris Kazaks / Svetlana Kotelovica / Inara Akopjana / Guido Pintacuda / Roman I Koning / Kaspars Tars / 要旨: AP205 is a single-stranded RNA bacteriophage that has a coat protein sequence not similar to any other known single-stranded RNA phage. Here, we report an atomic-resolution model of the AP205 virus- ...AP205 is a single-stranded RNA bacteriophage that has a coat protein sequence not similar to any other known single-stranded RNA phage. Here, we report an atomic-resolution model of the AP205 virus-like particle based on a crystal structure of an unassembled coat protein dimer and a cryo-electron microscopy reconstruction of the assembled particle, together with secondary structure information from site-specific solid-state NMR data. The AP205 coat protein dimer adopts the conserved Leviviridae coat protein fold except for the N-terminal region, which forms a beta-hairpin in the other known single-stranded RNA phages. AP205 has a similar structure at the same location formed by N- and C-terminal beta-strands, making it a circular permutant compared to the other coat proteins. The permutation moves the coat protein termini to the most surface-exposed part of the assembled particle, which explains its increased tolerance to long N- and C-terminal fusions.
AB: Coat protein AC: Coat protein AD: Coat protein AE: Coat protein AF: Coat protein AG: Coat protein AH: Coat protein AI: Coat protein AJ: Coat protein AK: Coat protein AL: Coat protein AM: Coat protein AN: Coat protein AO: Coat protein AP: Coat protein AQ: Coat protein AR: Coat protein AS: Coat protein AT: Coat protein AU: Coat protein AV: Coat protein AW: Coat protein AX: Coat protein AY: Coat protein AZ: Coat protein BA: Coat protein BB: Coat protein BC: Coat protein BD: Coat protein BE: Coat protein BF: Coat protein BG: Coat protein BH: Coat protein BI: Coat protein BJ: Coat protein BK: Coat protein BL: Coat protein BM: Coat protein BN: Coat protein BO: Coat protein BP: Coat protein BQ: Coat protein BR: Coat protein BS: Coat protein BT: Coat protein BU: Coat protein BV: Coat protein BW: Coat protein BX: Coat protein BY: Coat protein BZ: Coat protein CA: Coat protein CB: Coat protein CC: Coat protein CD: Coat protein CE: Coat protein CF: Coat protein CG: Coat protein CH: Coat protein CI: Coat protein CJ: Coat protein CK: Coat protein CL: Coat protein CM: Coat protein CN: Coat protein CO: Coat protein CP: Coat protein CQ: Coat protein CR: Coat protein CS: Coat protein CT: Coat protein CU: Coat protein CV: Coat protein CW: Coat protein CX: Coat protein CY: Coat protein CZ: Coat protein DA: Coat protein DB: Coat protein DC: Coat protein DD: Coat protein DE: Coat protein DF: Coat protein DG: Coat protein DH: Coat protein DI: Coat protein DJ: Coat protein DK: Coat protein DL: Coat protein DM: Coat protein DN: Coat protein DO: Coat protein DP: Coat protein DQ: Coat protein DR: Coat protein DS: Coat protein DT: Coat protein DU: Coat protein DV: Coat protein DW: Coat protein DX: Coat protein DY: Coat protein DZ: Coat protein EA: Coat protein EB: Coat protein EC: Coat protein ED: Coat protein EE: Coat protein EF: Coat protein EG: Coat protein EH: Coat protein EI: Coat protein EJ: Coat protein EK: Coat protein EL: Coat protein EM: Coat protein EN: Coat protein EO: Coat protein EP: Coat protein EQ: Coat protein ER: Coat protein ES: Coat protein ET: Coat protein EU: Coat protein EV: Coat protein EW: Coat protein EX: Coat protein EY: Coat protein EZ: Coat protein FA: Coat protein FB: Coat protein FC: Coat protein FD: Coat protein FE: Coat protein FF: Coat protein FG: Coat protein FH: Coat protein FI: Coat protein FJ: Coat protein FK: Coat protein FL: Coat protein FM: Coat protein FN: Coat protein FO: Coat protein FP: Coat protein FQ: Coat protein FR: Coat protein FS: Coat protein FT: Coat protein FU: Coat protein FV: Coat protein FW: Coat protein FX: Coat protein FY: Coat protein FZ: Coat protein GA: Coat protein GB: Coat protein GC: Coat protein GD: Coat protein GE: Coat protein GF: Coat protein GG: Coat protein GH: Coat protein GI: Coat protein GJ: Coat protein GK: Coat protein GL: Coat protein GM: Coat protein GN: Coat protein GO: Coat protein GP: Coat protein GQ: Coat protein GR: Coat protein GS: Coat protein GT: Coat protein GU: Coat protein GV: Coat protein GW: Coat protein GX: Coat protein GY: Coat protein