ジャーナル: Nat Struct Biol / 年: 2003 タイトル: Three-dimensional structure of bacteriophage T4 baseplate. 著者: Victor A Kostyuchenko / Petr G Leiman / Paul R Chipman / Shuji Kanamaru / Mark J van Raaij / Fumio Arisaka / Vadim V Mesyanzhinov / Michael G Rossmann / 要旨: The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three- ...The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 A by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection.
SEQUENCE Only coordinates for CA atoms were submitted.
Remark 300
BIOMOLECULE: 1 THIS ENTRY CONTAINS A PORTION OF THE BIOLOGICALLY SIGNIFICANT MULTIMER. ASSEMBLY ...BIOMOLECULE: 1 THIS ENTRY CONTAINS A PORTION OF THE BIOLOGICALLY SIGNIFICANT MULTIMER. ASSEMBLY COMPONENTS COM_ID: 1 NAME:GP8 IPR_ID: NULL GO_ID: NULL OTHER_DETAILS: TRIMER
A: Baseplate structural protein Gp8 B: Baseplate structural protein Gp8 C: Baseplate structural protein Gp8 D: Baseplate structural protein Gp8 E: Baseplate structural protein Gp8 F: Baseplate structural protein Gp8 G: Baseplate structural protein Gp8 H: Baseplate structural protein Gp8 I: Baseplate structural protein Gp8 J: Baseplate structural protein Gp8 K: Baseplate structural protein Gp8 L: Baseplate structural protein Gp8
モード: BRIGHT FIELD / 倍率(公称値): 45000 X / 倍率(補正後): 47000 X / 最大 デフォーカス(公称値): 5000 nm / 最小 デフォーカス(公称値): 1200 nm / Cs: 2 mm
試料ホルダ
温度: 70 K / 傾斜角・最大: 0 ° / 傾斜角・最小: 0 °
撮影
電子線照射量: 25 e/Å2 / フィルム・検出器のモデル: KODAK SO-163 FILM
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解析
EMソフトウェア
ID
名称
バージョン
カテゴリ
1
SITUS COLORES
2
モデルフィッティング
2
SPIDER
3次元再構成
CTF補正
詳細: CTF was corrected for individual particles
対称性
点対称性: C6 (6回回転対称)
3次元再構成
手法: model based projection matching / 解像度: 12 Å / 粒子像の数: 945 / ピクセルサイズ(公称値): 3.11 Å / ピクセルサイズ(実測値): 2.98 Å / 倍率補正: TMV images 詳細: modified version of SPIDER program was used for the reconstruction 対称性のタイプ: POINT
原子モデル構築
プロトコル: OTHER / 空間: REAL / Target criteria: Correlation coefficient / 詳細: REFINEMENT PROTOCOL--Laplacian filtered real space