ジャーナル: Neuron / 年: 2021 タイトル: Characterization of the subunit composition and structure of adult human glycine receptors. 著者: Hailong Yu / Xiao-Chen Bai / Weiwei Wang / 要旨: The strychnine-sensitive pentameric glycine receptor (GlyR) mediates fast inhibitory neurotransmission in the mammalian nervous system. Only heteromeric GlyRs mediate synaptic transmission, as they ...The strychnine-sensitive pentameric glycine receptor (GlyR) mediates fast inhibitory neurotransmission in the mammalian nervous system. Only heteromeric GlyRs mediate synaptic transmission, as they contain the β subunit that permits clustering at the synapse through its interaction with scaffolding proteins. Here, we show that α2 and β subunits assemble with an unexpected 4:1 stoichiometry to produce GlyR with native electrophysiological properties. We determined structures in multiple functional states at 3.6-3.8 Å resolutions and show how 4:1 stoichiometry is consistent with the structural features of α2β GlyR. Furthermore, we show that one single β subunit in each GlyR gives rise to the characteristic electrophysiological properties of heteromeric GlyR, while more β subunits render GlyR non-conductive. A single β subunit ensures a univalent GlyR-scaffold linkage, which means the scaffold alone regulates the cluster properties.
分子 #2: Glycine receptor subunit beta,Green fluorescent protein
分子
名称: Glycine receptor subunit beta,Green fluorescent protein タイプ: protein_or_peptide / ID: 2 詳細: This is a GFP insertion between helices M3-M4 of Glycine Receptor Beta コピー数: 1 / 光学異性体: LEVO