+
Open data
-
Basic information
Entry | Database: EMDB / ID: EMD-7352 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Cryo-EM structure of Orco | |||||||||
![]() | Odorant receptor | |||||||||
![]() |
| |||||||||
![]() | Olfactory receptor / Ion channel / Insect / Fab / MEMBRANE PROTEIN | |||||||||
Function / homology | Olfactory receptor, insect / 7tm Odorant receptor / olfactory receptor activity / odorant binding / signal transduction / identical protein binding / plasma membrane / Odorant receptor![]() | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.5 Å | |||||||||
![]() | Butterwick JA / Kim KH / Walz T / Ruta V | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: Cryo-EM structure of the insect olfactory receptor Orco. Authors: Joel A Butterwick / Josefina Del Mármol / Kelly H Kim / Martha A Kahlson / Jackson A Rogow / Thomas Walz / Vanessa Ruta / ![]() Abstract: The olfactory system must recognize and discriminate amongst an enormous variety of chemicals in the environment. To contend with such diversity, insects have evolved a family of odorant-gated ion ...The olfactory system must recognize and discriminate amongst an enormous variety of chemicals in the environment. To contend with such diversity, insects have evolved a family of odorant-gated ion channels comprised of a highly conserved co-receptor (Orco) and a divergent odorant receptor (OR) that confers chemical specificity. Here, we present the single-particle cryo-electron microscopy structure of an Orco homomer from the parasitic fig wasp Apocrypta bakeri at 3.5 Å resolution, providing structural insight into this receptor family. Orco possesses a novel channel architecture, with four subunits symmetrically arranged around a central pore that diverges into four lateral conduits that open to the cytosol. The Orco tetramer has few inter-subunit interactions within the membrane and is bound together by a small cytoplasmic anchor domain. The minimal sequence conservation among ORs maps largely to the pore and anchor domain, shedding light on how the architecture of this receptor family accommodates its remarkable sequence diversity and facilitates the evolution of odour tuning. | |||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-EMDB archive
Map data | ![]() | 179.4 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 9.8 KB 9.8 KB | Display Display | ![]() |
Images | ![]() | 79.6 KB | ||
Filedesc metadata | ![]() | 5.1 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 473 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 472.6 KB | Display | |
Data in XML | ![]() | 6.3 KB | Display | |
Data in CIF | ![]() | 7.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6c70MC M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data |
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Odorant receptor | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-
Sample components
-Entire : Orco homotetramer bound to four Fab molecules in a detergent micelle
Entire | Name: Orco homotetramer bound to four Fab molecules in a detergent micelle |
---|---|
Components |
|
-Supramolecule #1: Orco homotetramer bound to four Fab molecules in a detergent micelle
Supramolecule | Name: Orco homotetramer bound to four Fab molecules in a detergent micelle type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
---|---|
Source (natural) | Organism: ![]() |
-Macromolecule #1: Odorant receptor
Macromolecule | Name: Odorant receptor / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 53.513238 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: GPGRAKFKHQ GLVADLLPNI RVMQGVGHFM FNYYSEGKKF PHRIYCIVTL LLLLLQYGMM AVNLMMESDD VDDLTANTIT MLFFLHPIV KMIYFPVRSK IFYKTLAIWN NPNSHPLFAE SNARFHALAI TKMRRLLFCV AGATIFSVIS WTGITFIEDS V KRITDPET ...String: GPGRAKFKHQ GLVADLLPNI RVMQGVGHFM FNYYSEGKKF PHRIYCIVTL LLLLLQYGMM AVNLMMESDD VDDLTANTIT MLFFLHPIV KMIYFPVRSK IFYKTLAIWN NPNSHPLFAE SNARFHALAI TKMRRLLFCV AGATIFSVIS WTGITFIEDS V KRITDPET NETTIIPIPR LMIRTFYPFN AMSGAGHVFA LIYQFYYLVI SMAVSNSLDV LFCSWLLFAC EQLQHLKAIM KP LMELSAT LDTVVPNSGE LFKAGSADHL RESQGVQPSG NGDNVLDVDL RGIYSNRQDF TATFRPTAGT TFNGGVGPNG LTK KQEMLV RSAIKYWVER HKHVVRLVTA VGDAYGVALL LHMLTTTITL TLLAYQATKV NGVNVYAATV IGYLLYTLGQ VFLF CIFGN RLIEESSSVM EAAYSCHWYD GSEEAKTFVQ IVCQQCQKAM SISGAKFFTV SLDLFASVLG AVVTYFMVLV QLK UniProtKB: Odorant receptor |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Buffer | pH: 7.5 |
---|---|
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Frames/image: 1-50 / Number grids imaged: 1221 / Number real images: 53141 / Average exposure time: 0.2 sec. / Average electron dose: 80.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
-
Image processing
Startup model | Type of model: OTHER |
---|---|
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 53141 |
Initial angle assignment | Type: OTHER |
Final angle assignment | Type: OTHER |