[English] 日本語
![](img/lk-miru.gif)
- EMDB-5954: Protruding Knob-like Proteins Violate Local Symmetries in an Icos... -
+
Open data
-
Basic information
Entry | Database: EMDB / ID: EMD-5954 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Protruding Knob-like Proteins Violate Local Symmetries in an Icosahedral Marine Virus | |||||||||
![]() | Icosahedral cryo-EM reconstruction of Syn5 marine virus | |||||||||
![]() |
| |||||||||
![]() | Syn5 / marine / cyanophage / capsid proteins / symmetry break | |||||||||
Function / homology | Major capsid protein![]() | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.7 Å | |||||||||
![]() | Gipson P / Baker ML / Raytcheva D / Haase-Pettingell C / Piret J / King JA / Chiu W | |||||||||
![]() | ![]() Title: Protruding knob-like proteins violate local symmetries in an icosahedral marine virus. Authors: Preeti Gipson / Matthew L Baker / Desislava Raytcheva / Cameron Haase-Pettingell / Jacqueline Piret / Jonathan A King / Wah Chiu / ![]() Abstract: Marine viruses play crucial roles in shaping the dynamics of oceanic microbial communities and in the carbon cycle on Earth. Here we report a 4.7-Å structure of a cyanobacterial virus, Syn5, by ...Marine viruses play crucial roles in shaping the dynamics of oceanic microbial communities and in the carbon cycle on Earth. Here we report a 4.7-Å structure of a cyanobacterial virus, Syn5, by electron cryo-microscopy and modelling. A Cα backbone trace of the major capsid protein (gp39) reveals a classic phage protein fold. In addition, two knob-like proteins protruding from the capsid surface are also observed. Using bioinformatics and structure analysis tools, these proteins are identified to correspond to gp55 and gp58 (each with two copies per asymmetric unit). The non 1:1 stoichiometric distribution of gp55/58 to gp39 breaks all expected local symmetries and leads to non-quasi-equivalence of the capsid subunits, suggesting a role in capsid stabilization. Such a structural arrangement has not yet been observed in any known virus structures. | |||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-EMDB archive
Map data | ![]() | 68.7 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 8.7 KB 8.7 KB | Display Display | ![]() |
Images | ![]() ![]() | 129.3 KB 6.5 KB | ||
Filedesc structureFactors | ![]() | 2.5 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4bmlMC M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data |
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Icosahedral cryo-EM reconstruction of Syn5 marine virus | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.32 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-
Sample components
-Entire : Syn5 marine virus, also known as horned cyanophage Syn5
Entire | Name: Syn5 marine virus, also known as horned cyanophage Syn5 |
---|---|
Components |
|
-Supramolecule #1000: Syn5 marine virus, also known as horned cyanophage Syn5
Supramolecule | Name: Syn5 marine virus, also known as horned cyanophage Syn5 type: sample / ID: 1000 / Number unique components: 1 |
---|
-Supramolecule #1: Synechococcus phage Syn5
Supramolecule | Name: Synechococcus phage Syn5 / type: virus / ID: 1 / Name.synonym: Horned marine virus / NCBI-ID: 438482 / Sci species name: Synechococcus phage Syn5 / Sci species strain: Syn5 / Database: NCBI / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No / Syn species name: Horned marine virus |
---|---|
Host (natural) | Organism: ![]() |
Virus shell | Shell ID: 1 / Diameter: 655 Å / T number (triangulation number): 7 |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Grid | Details: R1.2/1.3 Quantifoil grids |
---|---|
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV / Method: 3 uL sample blotted once for 2.7 seconds |
-
Electron microscopy
Microscope | JEOL 3200FSC |
---|---|
Specialist optics | Energy filter - Name: Omega |
Date | Oct 10, 2010 |
Image recording | Category: CCD / Film or detector model: GATAN ULTRASCAN 10000 (10k x 10k) |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.7 µm / Nominal magnification: 80000 |
Sample stage | Specimen holder model: JEOL 3200FSC CRYOHOLDER |
-
Image processing
Final reconstruction | Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 4.7 Å / Resolution method: OTHER / Software - Name: MPSA / Number images used: 12000 |
---|