+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-3174 | |||||||||
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Title | P22 bacteriophage Empty Expanded Capsid | |||||||||
Map data | Reconstruction of bacteriophageP22 Empty Expanded Capsid | |||||||||
Sample |
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Keywords | P22 / bacteriophage / GFP / beta glucosidase / virus / AFM / cryoEM / cargo / nanocage | |||||||||
Biological species | Enterobacteria phage P22 (virus) | |||||||||
Method | single particle reconstruction / cryo EM / negative staining / Resolution: 14.5 Å | |||||||||
Authors | Llauro A / Luque D / Trus BL / Edwards E / Avera J / Reguera D / Douglas T / Pablo PJ / Caston JR | |||||||||
Citation | Journal: Nanoscale / Year: 2016 Title: Cargo-shell and cargo-cargo couplings govern the mechanics of artificially loaded virus-derived cages. Authors: Aida Llauró / Daniel Luque / Ethan Edwards / Benes L Trus / John Avera / David Reguera / Trevor Douglas / Pedro J de Pablo / José R Castón / Abstract: Nucleic acids are the natural cargo of viruses and key determinants that affect viral shell stability. In some cases the genome structurally reinforces the shell, whereas in others genome packaging ...Nucleic acids are the natural cargo of viruses and key determinants that affect viral shell stability. In some cases the genome structurally reinforces the shell, whereas in others genome packaging causes internal pressure that can induce destabilization. Although it is possible to pack heterologous cargoes inside virus-derived shells, little is known about the physical determinants of these artificial nanocontainers' stability. Atomic force and three-dimensional cryo-electron microscopy provided mechanical and structural information about the physical mechanisms of viral cage stabilization beyond the mere presence/absence of cargos. We analyzed the effects of cargo-shell and cargo-cargo interactions on shell stability after encapsulating two types of proteinaceous payloads. While bound cargo to the inner capsid surface mechanically reinforced the capsid in a structural manner, unbound cargo diffusing freely within the shell cavity pressurized the cages up to ∼30 atm due to steric effects. Strong cargo-cargo coupling reduces the resilience of these nanocompartments in ∼20% when bound to the shell. Understanding the stability of artificially loaded nanocages will help to design more robust and durable molecular nanocontainers. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_3174.map.gz | 20.8 MB | EMDB map data format | |
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Header (meta data) | emd-3174-v30.xml emd-3174.xml | 8.7 KB 8.7 KB | Display Display | EMDB header |
Images | emd_3174.png | 1.9 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-3174 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-3174 | HTTPS FTP |
-Validation report
Summary document | emd_3174_validation.pdf.gz | 280.3 KB | Display | EMDB validaton report |
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Full document | emd_3174_full_validation.pdf.gz | 279.4 KB | Display | |
Data in XML | emd_3174_validation.xml.gz | 6.3 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-3174 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-3174 | HTTPS FTP |
-Related structure data
Related structure data | 3171C 3172C 3173C 3175C 3176C 3177C C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_3174.map.gz / Format: CCP4 / Size: 21.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Reconstruction of bacteriophageP22 Empty Expanded Capsid | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 4.32 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Bacteriophage P22 Empty Expanded Capsid
Entire | Name: Bacteriophage P22 Empty Expanded Capsid |
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Components |
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-Supramolecule #1000: Bacteriophage P22 Empty Expanded Capsid
Supramolecule | Name: Bacteriophage P22 Empty Expanded Capsid / type: sample / ID: 1000 / Oligomeric state: 60 / Number unique components: 1 |
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-Supramolecule #1: Enterobacteria phage P22
Supramolecule | Name: Enterobacteria phage P22 / type: virus / ID: 1 / NCBI-ID: 10754 / Sci species name: Enterobacteria phage P22 / Virus type: VIRION / Virus isolate: OTHER / Virus enveloped: No / Virus empty: Yes |
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Host (natural) | Organism: Salmonella enterica subsp. enterica serovar Typhimurium (bacteria) synonym: BACTERIA(EUBACTERIA) |
Host system | Organism: Escherichia coli (E. coli) |
Virus shell | Shell ID: 1 / Diameter: 648 Å / T number (triangulation number): 7 |
-Experimental details
-Structure determination
Method | negative staining, cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Staining | Type: NEGATIVE Details: Samples were applied to grids, blotted and plunged into liquid ethane |
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Grid | Details: R 2/2 Quantifoil grids |
Vitrification | Cryogen name: ETHANE / Instrument: LEICA EM CPC |
-Electron microscopy
Microscope | FEI TECNAI F20 |
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Date | Dec 11, 2014 |
Image recording | Category: CCD / Film or detector model: FEI EAGLE (4k x 4k) / Digitization - Sampling interval: 15 µm / Number real images: 200 / Average electron dose: 10 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Calibrated magnification: 69444 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.26 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 0.75 µm / Nominal magnification: 69444 |
Sample stage | Specimen holder: Eucentric / Specimen holder model: GATAN LIQUID NITROGEN |
Experimental equipment | Model: Tecnai F20 / Image courtesy: FEI Company |
-Image processing
CTF correction | Details: Phase flipping & amplitude decay |
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Final reconstruction | Applied symmetry - Point group: I (icosahedral) / Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 14.5 Å / Resolution method: OTHER / Software - Name: Xmipp / Number images used: 7628 |