- EMDB-46448: CryoEM structure of PAR2 with endogenous tethered ligand. -
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基本情報
登録情報
データベース: EMDB / ID: EMD-46448
タイトル
CryoEM structure of PAR2 with endogenous tethered ligand.
マップデータ
試料
複合体: Complex of PAR2 with G proteins
タンパク質・ペプチド: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
タンパク質・ペプチド: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
タンパク質・ペプチド: scFv16
タンパク質・ペプチド: Proteinase-activated receptor 2
タンパク質・ペプチド: Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short chimera,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
キーワード
PAR2 / endogenous ligand / IMMUNE SYSTEM
機能・相同性
機能・相同性情報
positive regulation of neutrophil mediated killing of gram-negative bacterium / : / positive regulation of renin secretion into blood stream / positive regulation of eosinophil degranulation / mature conventional dendritic cell differentiation / leukocyte proliferation / positive regulation of glomerular filtration / regulation of chemokine (C-X-C motif) ligand 2 production / negative regulation of toll-like receptor 3 signaling pathway / positive regulation of toll-like receptor 3 signaling pathway ...positive regulation of neutrophil mediated killing of gram-negative bacterium / : / positive regulation of renin secretion into blood stream / positive regulation of eosinophil degranulation / mature conventional dendritic cell differentiation / leukocyte proliferation / positive regulation of glomerular filtration / regulation of chemokine (C-X-C motif) ligand 2 production / negative regulation of toll-like receptor 3 signaling pathway / positive regulation of toll-like receptor 3 signaling pathway / thrombin-activated receptor activity / positive regulation of actin filament depolymerization / positive regulation of toll-like receptor 2 signaling pathway / cell-cell junction maintenance / Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion / Acetylcholine regulates insulin secretion / phospholipase C-activating G protein-coupled glutamate receptor signaling pathway / positive regulation of toll-like receptor 4 signaling pathway / positive regulation of pseudopodium assembly / T cell activation involved in immune response / PLC beta mediated events / phospholipase C-activating serotonin receptor signaling pathway / positive regulation of cytokine production involved in immune response / entrainment of circadian clock / regulation of platelet activation / positive regulation of phagocytosis, engulfment / negative regulation of chemokine production / positive regulation of chemotaxis / neutrophil activation / positive regulation of leukocyte chemotaxis / establishment of endothelial barrier / regulation of JNK cascade / positive regulation of positive chemotaxis / regulation of canonical NF-kappaB signal transduction / regulation of canonical Wnt signaling pathway / negative regulation of JNK cascade / glutamate receptor signaling pathway / leukocyte migration / positive regulation of Rho protein signal transduction / regulation of blood coagulation / pseudopodium / positive regulation of interleukin-10 production / PKA activation in glucagon signalling / phototransduction, visible light / developmental growth / hair follicle placode formation / G-protein alpha-subunit binding / photoreceptor outer segment / negative regulation of tumor necrosis factor-mediated signaling pathway / neuropeptide signaling pathway / postsynaptic cytosol / D1 dopamine receptor binding / intracellular transport / vascular endothelial cell response to laminar fluid shear stress / renal water homeostasis / activation of adenylate cyclase activity / Hedgehog 'off' state / adenylate cyclase-activating adrenergic receptor signaling pathway / positive regulation of chemokine production / response to prostaglandin E / negative regulation of protein kinase activity / enzyme regulator activity / positive regulation of GTPase activity / cellular response to glucagon stimulus / regulation of insulin secretion / GTPase activator activity / Peptide ligand-binding receptors / adenylate cyclase activator activity / positive regulation of superoxide anion generation / Turbulent (oscillatory, disturbed) flow shear stress activates signaling by PIEZO1 and integrins in endothelial cells / positive regulation of interleukin-1 beta production / trans-Golgi network membrane / positive regulation of interleukin-8 production / positive regulation of JNK cascade / negative regulation of inflammatory response to antigenic stimulus / negative regulation of insulin secretion / G protein-coupled receptor binding / G protein-coupled receptor activity / bone development / positive regulation of interleukin-6 production / G-protein beta/gamma-subunit complex binding / platelet aggregation / Olfactory Signaling Pathway / Activation of the phototransduction cascade / cognition / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Thromboxane signalling through TP receptor / G protein-coupled acetylcholine receptor signaling pathway / G-protein activation / positive regulation of type II interferon production / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / adenylate cyclase-activating G protein-coupled receptor signaling pathway / Prostacyclin signalling through prostacyclin receptor / G beta:gamma signalling through CDC42 / Glucagon signaling in metabolic regulation / G beta:gamma signalling through BTK / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / ADP signalling through P2Y purinoceptor 12 類似検索 - 分子機能
Protease-activated receptor 2 / Protease-activated receptor / G-protein alpha subunit, group Q / G-protein alpha subunit, group S / Guanine nucleotide binding protein (G-protein), alpha subunit / G protein alpha subunit, helical insertion / G-protein alpha subunit / G-alpha domain profile. / G protein alpha subunit / G-protein, gamma subunit ...Protease-activated receptor 2 / Protease-activated receptor / G-protein alpha subunit, group Q / G-protein alpha subunit, group S / Guanine nucleotide binding protein (G-protein), alpha subunit / G protein alpha subunit, helical insertion / G-protein alpha subunit / G-alpha domain profile. / G protein alpha subunit / G-protein, gamma subunit / G-protein gamma subunit domain profile. / G-protein gamma-like domain / G-protein gamma-like domain superfamily / GGL domain / G protein gamma subunit-like motifs / GGL domain / G protein beta WD-40 repeat protein / Guanine nucleotide-binding protein, beta subunit / G-protein, beta subunit / G protein-coupled receptor, rhodopsin-like / GPCR, rhodopsin-like, 7TM / G-protein coupled receptors family 1 profile. / 7 transmembrane receptor (rhodopsin family) / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
Guanine nucleotide-binding protein G(q) subunit alpha / Proteinase-activated receptor 2 / Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 / Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 / Guanine nucleotide-binding protein G(s) subunit alpha isoforms short 類似検索 - 構成要素
ジャーナル: Nat Commun / 年: 2025 タイトル: Structural basis for the activation of proteinase-activated receptors PAR1 and PAR2. 著者: Zongyang Lyu / Xiaoxuan Lyu / Andrey G Malyutin / Guliang Xia / Daniel Carney / Vinicius M Alves / Matthew Falk / Nidhi Arora / Hua Zou / Aaron P McGrath / Yanyong Kang / 要旨: Members of the proteinase-activated receptor (PAR) subfamily of G protein-coupled receptors (GPCRs) play critical roles in processes like hemostasis, thrombosis, development, wound healing, ...Members of the proteinase-activated receptor (PAR) subfamily of G protein-coupled receptors (GPCRs) play critical roles in processes like hemostasis, thrombosis, development, wound healing, inflammation, and cancer progression. Comprising PAR1-PAR4, these receptors are specifically activated by protease cleavage at their extracellular amino terminus, revealing a 'tethered ligand' that self-activates the receptor. This triggers complex intracellular signaling via G proteins and beta-arrestins, linking external protease signals to cellular functions. To date, direct structural visualization of these ligand-receptor complexes has been limited. Here, we present structural snapshots of activated PAR1 and PAR2 bound to their endogenous tethered ligands, revealing a shallow and constricted orthosteric binding pocket. Comparisons with antagonist-bound structures show minimal conformational changes in the TM6 helix and larger movements of TM7 upon activation. These findings reveal a common activation mechanism for PAR1 and PAR2, highlighting critical residues involved in ligand recognition. Additionally, the structure of PAR2 bound to a pathway selective antagonist, GB88, demonstrates how potent orthosteric engagement can be achieved by a small molecule mimicking the endogenous tethered ligand's interactions.
タンパク質・ペプチド: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
タンパク質・ペプチド: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
タンパク質・ペプチド: scFv16
タンパク質・ペプチド: Proteinase-activated receptor 2
タンパク質・ペプチド: Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short chimera,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
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超分子 #1: Complex of PAR2 with G proteins
超分子
名称: Complex of PAR2 with G proteins / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all
由来(天然)
生物種: Homo sapiens (ヒト)
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分子 #1: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1