+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-42848 | |||||||||
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Title | AaegOR10 apo structure | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Mosquito olfactory receptor / MEMBRANE PROTEIN | |||||||||
Function / homology | Olfactory receptor, insect / 7tm Odorant receptor / olfactory receptor activity / odorant binding / signal transduction / identical protein binding / plasma membrane / Odorant receptor / Odorant receptor Function and homology information | |||||||||
Biological species | Aedes aegypti (yellow fever mosquito) / Apocrypta bakeri (insect) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.88 Å | |||||||||
Authors | Zhao J / del Marmol J | |||||||||
Funding support | United States, 2 items
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Citation | Journal: Science / Year: 2024 Title: Structural basis of odor sensing by insect heteromeric odorant receptors Authors: Zhao J / Chen AQ / Ryu J / del Marmol J | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_42848.map.gz | 46.9 MB | EMDB map data format | |
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Header (meta data) | emd-42848-v30.xml emd-42848.xml | 15.5 KB 15.5 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_42848_fsc.xml | 9.5 KB | Display | FSC data file |
Images | emd_42848.png | 181.8 KB | ||
Filedesc metadata | emd-42848.cif.gz | 5.8 KB | ||
Others | emd_42848_half_map_1.map.gz emd_42848_half_map_2.map.gz | 84.5 MB 84.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-42848 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-42848 | HTTPS FTP |
-Related structure data
Related structure data | 8v00MC 8v02C 8v3cC 8v3dC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_42848.map.gz / Format: CCP4 / Size: 91.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.827 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_42848_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_42848_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : AaegOR10 heterotetramer without ligand
Entire | Name: AaegOR10 heterotetramer without ligand |
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Components |
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-Supramolecule #1: AaegOR10 heterotetramer without ligand
Supramolecule | Name: AaegOR10 heterotetramer without ligand / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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-Supramolecule #2: Aedes aegypti OR10
Supramolecule | Name: Aedes aegypti OR10 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: Aedes aegypti (yellow fever mosquito) |
-Supramolecule #3: Apocrypta bakeri Orco
Supramolecule | Name: Apocrypta bakeri Orco / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2 |
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Source (natural) | Organism: Apocrypta bakeri (insect) |
-Macromolecule #1: Odorant receptor OR10
Macromolecule | Name: Odorant receptor OR10 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Aedes aegypti (yellow fever mosquito) |
Molecular weight | Theoretical: 43.491168 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: MASILDCPIV SVNARVWRFW SFVLKHDAMR YISIIPVTVM TFFMFTDLCR SWGNIQELII KAYFAVLYFN AVLRTLILVK DRKLYENFM QGISNVYFEI SHIDDHKIQS LLKSYTVRAR MLSISNLALG AIISTCFVVY PIFTGERGLP YGMFIPGLDS F RSPHYEII ...String: MASILDCPIV SVNARVWRFW SFVLKHDAMR YISIIPVTVM TFFMFTDLCR SWGNIQELII KAYFAVLYFN AVLRTLILVK DRKLYENFM QGISNVYFEI SHIDDHKIQS LLKSYTVRAR MLSISNLALG AIISTCFVVY PIFTGERGLP YGMFIPGLDS F RSPHYEII YIVQVVLTFP GCCMYIPFTS FFASTTLFGL VQIKTLQRQL QTFKDNINSQ DKEKVKAKVV KLIEDHKRII TY VSELNSL VTYICFVEFL SFGMMLCALL FLLNVIENHA QIVIVAAYIF MIISQIFAFY WHANEVREES MNLAEAAYSG PWV ELDNSI KKKLLLIILR AQQPLEITVG NVYPMTLEMF QSLLNASYSY FTLLRRVYN UniProtKB: Odorant receptor |
-Macromolecule #2: Odorant receptor Orco
Macromolecule | Name: Odorant receptor Orco / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: Apocrypta bakeri (insect) |
Molecular weight | Theoretical: 53.204953 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: MKFKHQGLVA DLLPNIRVMQ GVGHFMFNYY SEGKKFPHRI YCIVTLLLLL LQYGMMAVNL MMESDDVDDL TANTITMLFF LHPIVKMIY FPVRSKIFYK TLAIWNNPNS HPLFAESNAR FHALAITKMR RLLFCVAGAT IFSVISWTGI TFIEDSVKRI T DPETNETT ...String: MKFKHQGLVA DLLPNIRVMQ GVGHFMFNYY SEGKKFPHRI YCIVTLLLLL LQYGMMAVNL MMESDDVDDL TANTITMLFF LHPIVKMIY FPVRSKIFYK TLAIWNNPNS HPLFAESNAR FHALAITKMR RLLFCVAGAT IFSVISWTGI TFIEDSVKRI T DPETNETT IIPIPRLMIR TFYPFNAMSG AGHVFALIYQ FYYLVISMAV SNSLDVLFCS WLLFACEQLQ HLKAIMKPLM EL SATLDTV VPNSGELFKA GSADHLRESQ GVQPSGNGDN VLDVDLRGIY SNRQDFTATF RPTAGTTFNG GVGPNGLTKK QEM LVRSAI KYWVERHKHV VRLVTAVGDA YGVALLLHML TTTITLTLLA YQATKVNGVN VYAATVIGYL LYTLGQVFLF CIFG NRLIE ESSSVMEAAY SCHWYDGSEE AKTFVQIVCQ QCQKAMSISG AKFFTVSLDL FASVLGAVVT YFMVLVQLK UniProtKB: Odorant receptor |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |