+
データを開く
-
基本情報
登録情報 | ![]() | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
タイトル | Structure of the Human cytoplasmic Ribosome with human tRNA Tyr(GalQ34) and mRNA(UAU) (rotated state) | ||||||||||||
![]() | |||||||||||||
![]() |
| ||||||||||||
![]() | tRNA modifications / decoding / ![]() | ||||||||||||
機能・相同性 | ![]() positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis / negative regulation of endoplasmic reticulum unfolded protein response / eukaryotic 80S initiation complex / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / negative regulation of protein neddylation / protein tyrosine kinase inhibitor activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() 類似検索 - 分子機能 | ||||||||||||
生物種 | ![]() ![]() | ||||||||||||
手法 | ![]() ![]() | ||||||||||||
![]() | Ishiguro K / Yokoyama T / Shirouzu M / Suzuki T | ||||||||||||
資金援助 | ![]()
| ||||||||||||
![]() | ![]() タイトル: Glycosylated queuosines in tRNAs optimize translational rate and post-embryonic growth. 著者: Xuewei Zhao / Ding Ma / Kensuke Ishiguro / Hironori Saito / Shinichiro Akichika / Ikuya Matsuzawa / Mari Mito / Toru Irie / Kota Ishibashi / Kimi Wakabayashi / Yuriko Sakaguchi / Takeshi ...著者: Xuewei Zhao / Ding Ma / Kensuke Ishiguro / Hironori Saito / Shinichiro Akichika / Ikuya Matsuzawa / Mari Mito / Toru Irie / Kota Ishibashi / Kimi Wakabayashi / Yuriko Sakaguchi / Takeshi Yokoyama / Yuichiro Mishima / Mikako Shirouzu / Shintaro Iwasaki / Takeo Suzuki / Tsutomu Suzuki / ![]() 要旨: Transfer RNA (tRNA) modifications are critical for protein synthesis. Queuosine (Q), a 7-deaza-guanosine derivative, is present in tRNA anticodons. In vertebrate tRNAs for Tyr and Asp, Q is further ...Transfer RNA (tRNA) modifications are critical for protein synthesis. Queuosine (Q), a 7-deaza-guanosine derivative, is present in tRNA anticodons. In vertebrate tRNAs for Tyr and Asp, Q is further glycosylated with galactose and mannose to generate galQ and manQ, respectively. However, biogenesis and physiological relevance of Q-glycosylation remain poorly understood. Here, we biochemically identified two RNA glycosylases, QTGAL and QTMAN, and successfully reconstituted Q-glycosylation of tRNAs using nucleotide diphosphate sugars. Ribosome profiling of knockout cells revealed that Q-glycosylation slowed down elongation at cognate codons, UAC and GAC (GAU), respectively. We also found that galactosylation of Q suppresses stop codon readthrough. Moreover, protein aggregates increased in cells lacking Q-glycosylation, indicating that Q-glycosylation contributes to proteostasis. Cryo-EM of human ribosome-tRNA complex revealed the molecular basis of codon recognition regulated by Q-glycosylations. Furthermore, zebrafish qtgal and qtman knockout lines displayed shortened body length, implying that Q-glycosylation is required for post-embryonic growth in vertebrates. | ||||||||||||
履歴 |
|
-
構造の表示
添付画像 |
---|
-
ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 522.1 MB | ![]() | |
---|---|---|---|---|
ヘッダ (付随情報) | ![]() ![]() | 105 KB 105 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 18.7 KB | 表示 | ![]() |
画像 | ![]() | 76.6 KB | ||
Filedesc metadata | ![]() | 21.3 KB | ||
その他 | ![]() ![]() ![]() | 455.5 MB 456.3 MB 456.3 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8jdmMC ![]() 7y7cC ![]() 7y7dC ![]() 7y7eC ![]() 7y7fC ![]() 7y7gC ![]() 7y7hC ![]() 8jdjC ![]() 8jdkC ![]() 8jdlC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
---|---|
類似構造データ | 類似検索 - 機能・相同性 ![]() |
-
リンク
EMDBのページ | ![]() ![]() |
---|---|
「今月の分子」の関連する項目 |
-
マップ
ファイル | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ボクセルのサイズ | X=Y=Z: 0.83 Å | ||||||||||||||||||||
密度 |
| ||||||||||||||||||||
対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
|
-添付データ
-追加マップ: before postprocess
ファイル | emd_36181_additional_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
注釈 | before postprocess | ||||||||||||
投影像・断面図 |
| ||||||||||||
密度ヒストグラム |
-ハーフマップ: #2
ファイル | emd_36181_half_map_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
投影像・断面図 |
| ||||||||||||
密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_36181_half_map_2.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
投影像・断面図 |
| ||||||||||||
密度ヒストグラム |
-
試料の構成要素
+全体 : The complex of Human 80S Ribosome with mRNA and A-, P- site tRNA ...
+超分子 #1: The complex of Human 80S Ribosome with mRNA and A-, P- site tRNA ...
+分子 #1: mRNA
+分子 #2: tRNA (Tyr)
+分子 #3: 28S ribosomal RNA
+分子 #4: 5S ribosomal RNA
+分子 #5: 5.8S ribosomal RNA
+分子 #48: 18S ribosomal RNA
+分子 #6: 60S ribosomal protein L8
+分子 #7: 60S ribosomal protein L3
+分子 #8: 60S ribosomal protein L4
+分子 #9: 60S ribosomal protein L5
+分子 #10: 60S ribosomal protein L6
+分子 #11: 60S ribosomal protein L7
+分子 #12: 60S ribosomal protein L7a
+分子 #13: 60S ribosomal protein L9
+分子 #14: 60S ribosomal protein L10-like
+分子 #15: 60S ribosomal protein L11
+分子 #16: 60S ribosomal protein L13
+分子 #17: 60S ribosomal protein L14
+分子 #18: 60S ribosomal protein L15
+分子 #19: 60S ribosomal protein L13a
+分子 #20: 60S ribosomal protein L17
+分子 #21: 60S ribosomal protein L18
+分子 #22: 60S ribosomal protein L19
+分子 #23: 60S ribosomal protein L18a
+分子 #24: 60S ribosomal protein L21
+分子 #25: 60S ribosomal protein L22
+分子 #26: 60S ribosomal protein L23
+分子 #27: 60S ribosomal protein L24
+分子 #28: 60S ribosomal protein L23a
+分子 #29: 60S ribosomal protein L26
+分子 #30: 60S ribosomal protein L27
+分子 #31: 60S ribosomal protein L27a
+分子 #32: 60S ribosomal protein L29
+分子 #33: 60S ribosomal protein L30
+分子 #34: 60S ribosomal protein L31
+分子 #35: 60S ribosomal protein L32
+分子 #36: 60S ribosomal protein L35a
+分子 #37: 60S ribosomal protein L34
+分子 #38: 60S ribosomal protein L35
+分子 #39: 60S ribosomal protein L36
+分子 #40: 60S ribosomal protein L37
+分子 #41: 60S ribosomal protein L38
+分子 #42: 60S ribosomal protein L39
+分子 #43: Ubiquitin-60S ribosomal protein L40
+分子 #44: 60S ribosomal protein L41
+分子 #45: 60S ribosomal protein L36a
+分子 #46: 60S ribosomal protein L37a
+分子 #47: 60S ribosomal protein L28
+分子 #49: 40S ribosomal protein SA
+分子 #50: 40S ribosomal protein S3a
+分子 #51: 40S ribosomal protein S2
+分子 #52: 40S ribosomal protein S3
+分子 #53: 40S ribosomal protein S4, X isoform
+分子 #54: 40S ribosomal protein S5
+分子 #55: 40S ribosomal protein S6
+分子 #56: 40S ribosomal protein S7
+分子 #57: 40S ribosomal protein S8
+分子 #58: 40S ribosomal protein S9
+分子 #59: 40S ribosomal protein S10
+分子 #60: 40S ribosomal protein S11
+分子 #61: 40S ribosomal protein S13
+分子 #62: 40S ribosomal protein S14
+分子 #63: 40S ribosomal protein S15
+分子 #64: 40S ribosomal protein S16
+分子 #65: 40S ribosomal protein S17
+分子 #66: 40S ribosomal protein S18
+分子 #67: 40S ribosomal protein S19
+分子 #68: 40S ribosomal protein S20
+分子 #69: 40S ribosomal protein S21
+分子 #70: 40S ribosomal protein S15a
+分子 #71: 40S ribosomal protein S23
+分子 #72: 40S ribosomal protein S24
+分子 #73: 40S ribosomal protein S25
+分子 #74: 40S ribosomal protein S26
+分子 #75: 40S ribosomal protein S27
+分子 #76: 40S ribosomal protein S28
+分子 #77: 40S ribosomal protein S29
+分子 #78: 40S ribosomal protein S30
+分子 #79: Receptor of activated protein C kinase 1
+分子 #80: beta-D-galactopyranose
+分子 #81: MAGNESIUM ION
+分子 #82: ZINC ION
-実験情報
-構造解析
手法 | ![]() |
---|---|
![]() | ![]() |
試料の集合状態 | particle |
-
試料調製
緩衝液 | pH: 7.4 構成要素:
詳細: The Buffer pH was adjusted to 7.4 using KOH. | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV | ||||||||||||
詳細 | 24nM ribosomes were incubated with 500nM tRNA and mRNA |
-
電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
---|---|
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD![]() |
撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 撮影したグリッド数: 2 / 実像数: 32000 / 平均露光時間: 1.0 sec. / 平均電子線量: 50.0 e/Å2 |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |