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- EMDB-31351: cryo-EM structure of apo GPR158 -

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Basic information

Entry
Database: EMDB / ID: EMD-31351
Titlecryo-EM structure of apo GPR158
Map data
Sample
  • Complex: GPR158
    • Protein or peptide: Probable G-protein coupled receptor 158
Function / homology
Function and homology information


G protein-coupled glycine receptor activity / regulation of G protein-coupled receptor signaling pathway / positive regulation of neurotransmitter secretion / regulation of synapse organization / enzyme activator activity / cell projection / protein localization to plasma membrane / brain development / cognition / transmembrane signaling receptor activity ...G protein-coupled glycine receptor activity / regulation of G protein-coupled receptor signaling pathway / positive regulation of neurotransmitter secretion / regulation of synapse organization / enzyme activator activity / cell projection / protein localization to plasma membrane / brain development / cognition / transmembrane signaling receptor activity / presynaptic membrane / postsynaptic membrane / G protein-coupled receptor signaling pathway / nucleus / plasma membrane
Similarity search - Function
G-protein coupled receptor 158/179 / GPCR family 3, C-terminal / 7 transmembrane sweet-taste receptor of 3 GCPR / G-protein coupled receptors family 3 profile.
Similarity search - Domain/homology
Metabotropic glycine receptor
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.52 Å
AuthorsJeong E / Kim Y / Jeong J / Cho Y
Funding support Korea, Republic Of, 3 items
OrganizationGrant numberCountry
National Research Foundation (NRF, Korea)2021R1A2C301335711 Korea, Republic Of
National Research Foundation (NRF, Korea)2017M3A9F6029736 Korea, Republic Of
National Research Foundation (NRF, Korea)2021R1A6A1A10042944 Korea, Republic Of
CitationJournal: Nat Commun / Year: 2021
Title: Structure of the class C orphan GPCR GPR158 in complex with RGS7-Gβ5.
Authors: Eunyoung Jeong / Yoojoong Kim / Jihong Jeong / Yunje Cho /
Abstract: GPR158, a class C orphan GPCR, functions in cognition, stress-induced mood control, and synaptic development. Among class C GPCRs, GPR158 is unique as it lacks a Venus flytrap-fold ligand-binding ...GPR158, a class C orphan GPCR, functions in cognition, stress-induced mood control, and synaptic development. Among class C GPCRs, GPR158 is unique as it lacks a Venus flytrap-fold ligand-binding domain and terminates Gαi/o protein signaling through the RGS7-Gβ5 heterodimer. Here, we report the cryo-EM structures of GPR158 alone and in complex with one or two RGS7-Gβ5 heterodimers. GPR158 dimerizes through Per-Arnt-Sim-fold extracellular and transmembrane (TM) domains connected by an epidermal growth factor-like linker. The TM domain (TMD) reflects both inactive and active states of other class C GPCRs: a compact intracellular TMD, conformations of the two intracellular loops (ICLs) and the TMD interface formed by TM4/5. The ICL2, ICL3, TM3, and first helix of the cytoplasmic coiled-coil provide a platform for the DHEX domain of one RGS7 and the second helix recruits another RGS7. The unique features of the RGS7-binding site underlie the selectivity of GPR158 for RGS7.
History
DepositionMay 25, 2021-
Header (metadata) releaseDec 1, 2021-
Map releaseDec 1, 2021-
UpdateFeb 16, 2022-
Current statusFeb 16, 2022Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.012
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 0.012
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7ewl
  • Surface level: 0.012
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_31351.png

Downloads & links

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Map

FileDownload / File: emd_31351.map.gz / Format: CCP4 / Size: 113.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 0.83 Å
Density
Contour LevelBy AUTHOR: 0.012 / Movie #1: 0.012
Minimum - Maximum-0.07422876 - 0.11969404
Average (Standard dev.)0.000119012264 (±0.0019084729)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions310310310
Spacing310310310
CellA=B=C: 257.3 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.830.830.83
M x/y/z310310310
origin x/y/z0.0000.0000.000
length x/y/z257.300257.300257.300
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS310310310
D min/max/mean-0.0740.1200.000

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Supplemental data

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Sample components

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Entire : GPR158

EntireName: GPR158
Components
  • Complex: GPR158
    • Protein or peptide: Probable G-protein coupled receptor 158

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Supramolecule #1: GPR158

SupramoleculeName: GPR158 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Homo sapiens (human) / Recombinant cell: HEK293
Molecular weightTheoretical: 200 kDa/nm

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Macromolecule #1: Probable G-protein coupled receptor 158

MacromoleculeName: Probable G-protein coupled receptor 158 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 77.462703 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: ASRDPQGRPD SPRERTPKGK PHAQQPGRAS ASDSSAPWSR STDGTILAQK LAEEVPMDVA SYLYTGDSHQ LKRANCSGRY ELAGLPGKW PALASAHPSL HRALDTLTHA TNFLNVMLQS NKSREQNLQD DLDWYQALVW SLLEGEPSIS RAAITFSTDS L SAPAPQVF ...String:
ASRDPQGRPD SPRERTPKGK PHAQQPGRAS ASDSSAPWSR STDGTILAQK LAEEVPMDVA SYLYTGDSHQ LKRANCSGRY ELAGLPGKW PALASAHPSL HRALDTLTHA TNFLNVMLQS NKSREQNLQD DLDWYQALVW SLLEGEPSIS RAAITFSTDS L SAPAPQVF LQATREESRI LLQDLSSSAP HLANATLETE WFHGLRRKWR PHLHRRGPNQ GPRGLGHSWR RKDGLGGDKS HF KWSPPYL ECENGSYKPG WLVTLSSAIY GLQPNLVPEF RGVMKVDINL QKVDIDQCSS DGWFSGTHKC HLNNSECMPI KGL GFVLGA YECICKAGFY HPGVLPVNNF RRRGPDQHIS GSTKDVSEEA YVCLPCREGC PFCADDSPCF VQEDKYLRLA IISF QALCM LLDFVSMLVV YHFRKAKSIR ASGLILLETI LFGSLLLYFP VVILYFEPST FRCILLRWAR LLGFATVYGT VTLKL HRVL KVFLSRTAQR IPYMTGGRVM RMLAVILLVV FWFLIGWTSS VCQNLEKQIS LIGQGKTSDH LIFNMCLIDR WDYMTA VAE FLFLLWGVYL CYAVRTVPSA FHEPRYMAVA VHNELIISAI FHTIRFVLAS RLQSDWMLML YFAHTHLTVT VTIGLLL IP KFSHSSNNPR DDIATEAYED ELDMGRSGSY LNSSINSAWS EHSLD

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration12.7 mg/mL
BufferpH: 7.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 %

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: DIFFRACTION
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: OTHER
Final angle assignmentType: OTHER
Final reconstructionNumber classes used: 1 / Resolution.type: BY AUTHOR / Resolution: 3.52 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 425819

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