登録情報 データベース : EMDB / ID : EMD-24844 構造の表示 ダウンロードとリンクタイトル Human KATP channel in open conformation, focused on Kir and one SUR, position 3 マップデータfocused on Kir and one SUR after sharpening (B-factor: 53.4), position 3 詳細 試料複合体 : Human KATP channel composed of Kir6.2 (C166S G334D) and SUR1複合体 : Kir6.2 (C166S G334D) subunit of human KATPタンパク質・ペプチド : ATP-sensitive inward rectifier potassium channel 11複合体 : SUR1 subunit of human KATPタンパク質・ペプチド : ATP-binding cassette sub-family C member 8リガンド : ADENOSINE-5'-DIPHOSPHATEリガンド : MAGNESIUM IONリガンド : ADENOSINE-5'-TRIPHOSPHATE 詳細機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
negative regulation of neuroblast migration / positive regulation of uterine smooth muscle relaxation / ATP sensitive Potassium channels / Defective ABCC8 can cause hypo- and hyper-glycemias / positive regulation of tight junction disassembly / potassium ion-transporting ATPase complex / negative regulation of blood-brain barrier permeability / response to resveratrol / ATP-activated inward rectifier potassium channel activity / glutamate secretion, neurotransmission ... negative regulation of neuroblast migration / positive regulation of uterine smooth muscle relaxation / ATP sensitive Potassium channels / Defective ABCC8 can cause hypo- and hyper-glycemias / positive regulation of tight junction disassembly / potassium ion-transporting ATPase complex / negative regulation of blood-brain barrier permeability / response to resveratrol / ATP-activated inward rectifier potassium channel activity / glutamate secretion, neurotransmission / inward rectifying potassium channel / negative regulation of low-density lipoprotein particle clearance / cell body fiber / sulfonylurea receptor activity / ventricular cardiac muscle tissue development / CAMKK-AMPK signaling cascade / positive regulation of potassium ion transport / ATPase-coupled monoatomic cation transmembrane transporter activity / response to pH / regulation of monoatomic ion transmembrane transport / inorganic cation transmembrane transport / neuromuscular process / ankyrin binding / action potential / negative regulation of glial cell proliferation / response to ATP / : / response to testosterone / response to zinc ion / potassium channel activity / intracellular glucose homeostasis / potassium ion import across plasma membrane / positive regulation of insulin secretion involved in cellular response to glucose stimulus / intercalated disc / ATPase-coupled transmembrane transporter activity / axolemma / negative regulation of insulin secretion / ABC-type transporter activity / potassium ion transmembrane transport / T-tubule / heat shock protein binding / negative regulation of angiogenesis / acrosomal vesicle / Regulation of insulin secretion / response to ischemia / female pregnancy / determination of adult lifespan / cellular response to glucose stimulus / positive regulation of protein localization to plasma membrane / visual learning / potassium ion transport / response to insulin / transmembrane transport / sarcolemma / memory / ADP binding / synaptic vesicle membrane / cellular response to nicotine / positive regulation of tumor necrosis factor production / response to estradiol / nuclear envelope / presynaptic membrane / cellular response to tumor necrosis factor / transmembrane transporter binding / response to lipopolysaccharide / response to hypoxia / endosome / response to xenobiotic stimulus / neuronal cell body / glutamatergic synapse / apoptotic process / ATP hydrolysis activity / ATP binding / membrane / plasma membrane 類似検索 - 分子機能 Potassium channel, inwardly rectifying, Kir6.2 / ATP-binding cassette subfamily C member 8 / Sulphonylurea receptor / Potassium channel, inwardly rectifying, transmembrane domain / Inward rectifier potassium channel transmembrane domain / Potassium channel, inwardly rectifying, Kir, cytoplasmic / Potassium channel, inwardly rectifying, Kir / Inward rectifier potassium channel, C-terminal / Inward rectifier potassium channel C-terminal domain / ABC transporter transmembrane region ... Potassium channel, inwardly rectifying, Kir6.2 / ATP-binding cassette subfamily C member 8 / Sulphonylurea receptor / Potassium channel, inwardly rectifying, transmembrane domain / Inward rectifier potassium channel transmembrane domain / Potassium channel, inwardly rectifying, Kir, cytoplasmic / Potassium channel, inwardly rectifying, Kir / Inward rectifier potassium channel, C-terminal / Inward rectifier potassium channel C-terminal domain / ABC transporter transmembrane region / ABC transporter type 1, transmembrane domain / ABC transporter integral membrane type-1 fused domain profile. / ABC transporter type 1, transmembrane domain superfamily / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / Immunoglobulin E-set / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性 ATP-sensitive inward rectifier potassium channel 11 / ATP-binding cassette sub-family C member 8 類似検索 - 構成要素生物種 Homo sapiens (ヒト)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.9 Å 詳細 データ登録者Zhao C / MacKinnon R 資金援助 米国, 1件 詳細 詳細を隠すOrganization Grant number 国 Howard Hughes Medical Institute (HHMI) 米国
引用ジャーナル : Proc Natl Acad Sci U S A / 年 : 2021タイトル : Molecular structure of an open human K channel.著者 : Chen Zhao / Roderick MacKinnon / 要旨 : K channels are metabolic sensors that translate intracellular ATP/ADP balance into membrane excitability. The molecular composition of K includes an inward-rectifier potassium channel (Kir) and an ... K channels are metabolic sensors that translate intracellular ATP/ADP balance into membrane excitability. The molecular composition of K includes an inward-rectifier potassium channel (Kir) and an ABC transporter-like sulfonylurea receptor (SUR). Although structures of K have been determined in many conformations, in all cases, the pore in Kir is closed. Here, we describe human pancreatic K (hK) structures with an open pore at 3.1- to 4.0-Å resolution using single-particle cryo-electron microscopy (cryo-EM). Pore opening is associated with coordinated structural changes within the ATP-binding site and the channel gate in Kir. Conformational changes in SUR are also observed, resulting in an area reduction of contact surfaces between SUR and Kir. We also observe that pancreatic hK exhibits the unique (among inward-rectifier channels) property of PIP-independent opening, which appears to be correlated with a docked cytoplasmic domain in the absence of PIP. 履歴 登録 2021年9月12日 - ヘッダ(付随情報) 公開 2021年12月1日 - マップ公開 2021年12月1日 - 更新 2021年12月8日 - 現状 2021年12月8日 処理サイト : RCSB / 状態 : 公開
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