+
Open data
-
Basic information
Entry | Database: EMDB / ID: EMD-22340 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Type IV-B CRISPR Complex | |||||||||
![]() | ||||||||||
![]() |
| |||||||||
![]() | CRISPR Complex / IMMUNE SYSTEM-RNA complex | |||||||||
Function / homology | CRISPR type III-associated protein / RAMP superfamily / defense response to virus / Uncharacterized protein / Uncharacterized protein![]() | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.9 Å | |||||||||
![]() | Bravo JPK / Taylor DW | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: Structure of a type IV CRISPR-Cas ribonucleoprotein complex. Authors: Yi Zhou / Jack P K Bravo / Hannah N Taylor / Jurre A Steens / Ryan N Jackson / Raymond H J Staals / David W Taylor / ![]() ![]() Abstract: We reveal the cryo-electron microscopy structure of a type IV-B CRISPR ribonucleoprotein (RNP) complex (Csf) at 3.9-Å resolution. The complex best resembles the type III-A CRISPR Csm effector ...We reveal the cryo-electron microscopy structure of a type IV-B CRISPR ribonucleoprotein (RNP) complex (Csf) at 3.9-Å resolution. The complex best resembles the type III-A CRISPR Csm effector complex, consisting of a Cas7-like (Csf2) filament intertwined with a small subunit (Cas11) filament, but the complex lacks subunits for RNA processing and target DNA cleavage. Surprisingly, instead of assembling around a CRISPR-derived RNA (crRNA), the complex assembles upon heterogeneous RNA of a regular length arranged in a pseudo-A-form configuration. These findings provide a high-resolution glimpse into the assembly and function of enigmatic type IV CRISPR systems, expanding our understanding of class I CRISPR-Cas system architecture, and suggesting a function for type IV-B RNPs that may be distinct from other class 1 CRISPR-associated systems. | |||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-EMDB archive
Map data | ![]() | 1.8 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 11 KB 11 KB | Display Display | ![]() |
Images | ![]() | 86 KB | ||
Filedesc metadata | ![]() | 5.2 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 349.8 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 349.4 KB | Display | |
Data in XML | ![]() | 6.1 KB | Display | |
Data in CIF | ![]() | 7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7jhyMC M: atomic model generated by this map C: citing same article ( |
---|---|
Similar structure data |
-
Links
EMDB pages | ![]() ![]() |
---|
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 1.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-
Sample components
-Entire : Csf CRISPR Complex
Entire | Name: Csf CRISPR Complex |
---|---|
Components |
|
-Supramolecule #1: Csf CRISPR Complex
Supramolecule | Name: Csf CRISPR Complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 |
---|---|
Source (natural) | Organism: ![]() |
-Macromolecule #1: Csf2 (Cas7)
Macromolecule | Name: Csf2 (Cas7) / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 32.085174 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MSWSHPQFEK GAMTLSTIRW DVDIIAQSSI VHRDDYTSVG SDTFTLFRRE KIIGPDGQIL QIPLISGSSF RGVLRRVGEA LTAEVLGYE DVALPVPAAH LLTNGGRLAK SAHPLTDEEE RNLKELLPQI AVFGGAASGR VMSGLLSVSK VLPEIAELAH L LPRPPHST ...String: MSWSHPQFEK GAMTLSTIRW DVDIIAQSSI VHRDDYTSVG SDTFTLFRRE KIIGPDGQIL QIPLISGSSF RGVLRRVGEA LTAEVLGYE DVALPVPAAH LLTNGGRLAK SAHPLTDEEE RNLKELLPQI AVFGGAASGR VMSGLLSVSK VLPEIAELAH L LPRPPHST PLLPAVLSVA DESFTHLPDH RPSTGGAPRT DHADGSPLGR FAIETLPAGT RLQTWARLDN ATEHQAAFFD NV LSTFAAH GHLGGRSAAG HGQVTATVTA TALRGSLPRP TVDWVNQLAD DRDAAIAALT RLT UniProtKB: Uncharacterized protein |
-Macromolecule #3: Csf4 (Cas11)
Macromolecule | Name: Csf4 (Cas11) / type: protein_or_peptide / ID: 3 / Number of copies: 5 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 17.939254 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MTTPTPTQVW RATVPELPPL VDEAGDTGSA TARAADTAER LLLLLHYSID WESSWVADPK HRKTYWDELL PGRVRRAAYR ADTLDRWWS EVAGQLGAPA PRHRDRRLEL ATLLREPALP VITVLRDSLP ALLLRVRIIA EAVAAQRGNN SAATSSADPN E PA UniProtKB: Uncharacterized protein |
-Macromolecule #2: RNA (31-MER)
Macromolecule | Name: RNA (31-MER) / type: rna / ID: 2 / Number of copies: 1 |
---|---|
Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 9.561382 KDa |
Sequence | String: AUUUUUUUUU AUUAUUUUUA UUUUUUAUUU U |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Concentration | 0.3 mg/mL |
---|---|
Buffer | pH: 8 |
Grid | Details: unspecified |
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
-
Image processing
Startup model | Type of model: NONE |
---|---|
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 296319 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |