- EMDB-20589: CryoEM reconstruction of membrane-bound ESCRT-III filament compos... -
+
データを開く
IDまたはキーワード:
読み込み中...
-
基本情報
登録情報
データベース: EMDB / ID: EMD-20589
タイトル
CryoEM reconstruction of membrane-bound ESCRT-III filament composed of CHMP1B+IST1 (left-handed)
マップデータ
RELION postprocessed map with helical symmetry applied to the entire map
試料
複合体: membrane-bound ESCRT-III copolymer filament composed of CHMP1B and IST1
タンパク質・ペプチド: Charged multivesicular body protein 1b
タンパク質・ペプチド: IST1 homolog
キーワード
membrane remodeling / membrane-bound protein filament / ESCRT-III / LIPID BINDING PROTEIN
機能・相同性
機能・相同性情報
viral capsid secondary envelopment / MIT domain binding / abscission / amphisome membrane / multivesicular body-lysosome fusion / vesicle fusion with vacuole / ESCRT III complex disassembly / late endosome to lysosome transport / ESCRT III complex / kinetochore microtubule ...viral capsid secondary envelopment / MIT domain binding / abscission / amphisome membrane / multivesicular body-lysosome fusion / vesicle fusion with vacuole / ESCRT III complex disassembly / late endosome to lysosome transport / ESCRT III complex / kinetochore microtubule / endosome transport via multivesicular body sorting pathway / cytoskeleton-dependent cytokinesis / regulation of centrosome duplication / collateral sprouting / nuclear membrane reassembly / positive regulation of collateral sprouting / Sealing of the nuclear envelope (NE) by ESCRT-III / midbody abscission / multivesicular body sorting pathway / membrane coat / plasma membrane repair / membrane fission / late endosome to vacuole transport / multivesicular body membrane / ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / multivesicular body assembly / regulation of mitotic spindle assembly / Flemming body / mitotic metaphase chromosome alignment / nucleus organization / viral budding via host ESCRT complex / autophagosome membrane / positive regulation of proteolysis / endoplasmic reticulum-Golgi intermediate compartment / autophagosome maturation / viral release from host cell / nuclear pore / multivesicular body / viral budding from plasma membrane / establishment of protein localization / kinetochore / autophagy / nuclear envelope / azurophil granule lumen / protein localization / protein transport / midbody / endosome membrane / cadherin binding / protein domain specific binding / cell division / lysosomal membrane / intracellular membrane-bounded organelle / centrosome / Neutrophil degranulation / chromatin / protein-containing complex binding / extracellular exosome / extracellular region / nucleoplasm / identical protein binding / plasma membrane / cytosol 類似検索 - 分子機能
Vacuolar protein sorting-associated protein Ist1 / Regulator of Vps4 activity in the MVB pathway / Vacuolar protein sorting-associated protein IST1-like / Snf7 family / Snf7 類似検索 - ドメイン・相同性
IST1 homolog / Charged multivesicular body protein 1b 類似検索 - 構成要素
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
P50 AI150464
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
1DP2GM110772-01
米国
National Institutes of Health/Office of the Director
1S10OD021596-01
米国
National Institutes of Health/Office of the Director
S10OD021741
米国
National Institutes of Health/Office of the Director
S10OD020054
米国
引用
ジャーナル: Nat Struct Mol Biol / 年: 2020 タイトル: Membrane constriction and thinning by sequential ESCRT-III polymerization. 著者: Henry C Nguyen / Nathaniel Talledge / John McCullough / Abhimanyu Sharma / Frank R Moss / Janet H Iwasa / Michael D Vershinin / Wesley I Sundquist / Adam Frost / 要旨: The endosomal sorting complexes required for transport (ESCRTs) mediate diverse membrane remodeling events. These typically require ESCRT-III proteins to stabilize negatively curved membranes; ...The endosomal sorting complexes required for transport (ESCRTs) mediate diverse membrane remodeling events. These typically require ESCRT-III proteins to stabilize negatively curved membranes; however, recent work has indicated that certain ESCRT-IIIs also participate in positive-curvature membrane-shaping reactions. ESCRT-IIIs polymerize into membrane-binding filaments, but the structural basis for negative versus positive membrane remodeling by these proteins remains poorly understood. To learn how certain ESCRT-IIIs shape positively curved membranes, we determined structures of human membrane-bound CHMP1B-only, membrane-bound CHMP1B + IST1, and IST1-only filaments by cryo-EM. Our structures show how CHMP1B first polymerizes into a single-stranded helical filament, shaping membranes into moderate-curvature tubules. Subsequently, IST1 assembles a second strand on CHMP1B, further constricting the membrane tube and reducing its diameter nearly to the fission point. Each step of constriction thins the underlying bilayer, lowering the barrier to membrane fission. Our structures reveal how a two-component, sequential polymerization mechanism drives membrane tubulation, constriction and bilayer thinning.
EMPIAR-10395 (タイトル: Helical Reconstruction of a left-handed and membrnae-bound ESCRT-III copolymer comprised of human CHMP1B and IST1 Data size: 26.8 Data #1: copolymer_LH_job1415.mrcs [picked particles - multiframe - processed])
凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 292 K / 装置: FEI VITROBOT MARK III 詳細: Grids were blotted with Whatman No. 1 filter paper for 4-8 seconds with a 0 mm offset at 19C and 100 percent humidity before plunging into liquid ethane.