+
データを開く
-
基本情報
登録情報 | ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
タイトル | Structure of an endogenous human TREX complex bound to mRNA, composite map | |||||||||
![]() | TREX-mRNA composite map generated from sharpened focused maps A (EMD 14805),B (EMD-14806) and C (EMD-14807 ) | |||||||||
![]() |
| |||||||||
機能・相同性 | ![]() THO complex / THO complex part of transcription export complex / transcription export complex / primitive hemopoiesis / C5-methylcytidine-containing RNA reader activity / regulation of mRNA export from nucleus / U6 snRNP / RNA secondary structure unwinding / stem cell division / Transport of the SLBP independent Mature mRNA ...THO complex / THO complex part of transcription export complex / transcription export complex / primitive hemopoiesis / C5-methylcytidine-containing RNA reader activity / regulation of mRNA export from nucleus / U6 snRNP / RNA secondary structure unwinding / stem cell division / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / mRNA 3'-end processing / ATP-dependent protein binding / Transport of Mature mRNA Derived from an Intronless Transcript / ATP-dependent activity, acting on RNA / U4 snRNA binding / Transport of Mature mRNA derived from an Intron-Containing Transcript / positive regulation of DNA-templated transcription, elongation / RNA export from nucleus / U4 snRNP / RNA Polymerase II Transcription Termination / poly(A)+ mRNA export from nucleus / generation of neurons / spliceosomal complex assembly / monocyte differentiation / blastocyst development / neuron development / RHOBTB2 GTPase cycle / U6 snRNA binding / mRNA export from nucleus / catalytic step 2 spliceosome / mRNA Splicing - Major Pathway / RNA splicing / regulation of DNA-templated transcription elongation / central nervous system development / spliceosomal complex / cell morphogenesis / mRNA processing / nuclear matrix / mRNA splicing, via spliceosome / osteoblast differentiation / Signaling by CSF1 (M-CSF) in myeloid cells / negative regulation of neuron projection development / regulation of gene expression / RNA helicase activity / nuclear body / RNA helicase / nuclear speck / mRNA binding / apoptotic process / signal transduction / ATP hydrolysis activity / DNA binding / RNA binding / extracellular exosome / nucleoplasm / ATP binding / membrane / identical protein binding / nucleus / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.9 Å | |||||||||
![]() | Pacheco-Fiallos FB / Vorlaender MK / Plaschka C | |||||||||
資金援助 | European Union, 2件
| |||||||||
![]() | ![]() タイトル: mRNA recognition and packaging by the human transcription-export complex. 著者: Belén Pacheco-Fiallos / Matthias K Vorländer / Daria Riabov-Bassat / Laura Fin / Francis J O'Reilly / Farja I Ayala / Ulla Schellhaas / Juri Rappsilber / Clemens Plaschka / ![]() ![]() ![]() 要旨: Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription-export complex (TREX) for nuclear export. However, the ...Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription-export complex (TREX) for nuclear export. However, the mechanisms of mRNP recognition and three-dimensional mRNP organization are poorly understood. Here we report cryo-electron microscopy and tomography structures of reconstituted and endogenous human mRNPs bound to the 2-MDa TREX complex. We show that mRNPs are recognized through multivalent interactions between the TREX subunit ALYREF and mRNP-bound exon junction complexes. Exon junction complexes can multimerize through ALYREF, which suggests a mechanism for mRNP organization. Endogenous mRNPs form compact globules that are coated by multiple TREX complexes. These results reveal how TREX may simultaneously recognize, compact and protect mRNAs to promote their packaging for nuclear export. The organization of mRNP globules provides a framework to understand how mRNP architecture facilitates mRNA biogenesis and export. | |||||||||
履歴 |
|
-
構造の表示
添付画像 |
---|
-
ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 8.2 MB | ![]() | |
---|---|---|---|---|
ヘッダ (付随情報) | ![]() ![]() | 23.7 KB 23.7 KB | 表示 表示 | ![]() |
画像 | ![]() | 118.2 KB | ||
その他 | ![]() | 8.3 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 360.3 KB | 表示 | ![]() |
---|---|---|---|---|
文書・詳細版 | ![]() | 359.8 KB | 表示 | |
XML形式データ | ![]() | 7.3 KB | 表示 | |
CIF形式データ | ![]() | 8.4 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7znkMC ![]() 7znjC ![]() 7znlC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
---|---|
類似構造データ | 類似検索 - 機能・相同性 ![]() |
-
リンク
EMDBのページ | ![]() ![]() |
---|---|
「今月の分子」の関連する項目 |
-
マップ
ファイル | ![]() | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
注釈 | TREX-mRNA composite map generated from sharpened focused maps A (EMD 14805),B (EMD-14806) and C (EMD-14807 ) | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.41 Å | ||||||||||||||||||||
密度 |
| ||||||||||||||||||||
対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
|
-添付データ
-追加マップ: TREX-mRNA composite map generated from unsharpened focused maps...
ファイル | emd_14804_additional_1.map | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
注釈 | TREX-mRNA composite map generated from unsharpened focused maps A (EMD 14805),B (EMD-14806) and C (EMD-14807 | ||||||||||||
投影像・断面図 |
| ||||||||||||
密度ヒストグラム |
-
試料の構成要素
+全体 : endogenous human TREX-mRNA complex
+超分子 #1: endogenous human TREX-mRNA complex
+分子 #1: RNA
+分子 #2: THO complex subunit 1
+分子 #3: THO complex subunit 2
+分子 #4: THO complex subunit 3
+分子 #5: THO complex subunit 5 homolog
+分子 #6: THO complex subunit 6 homolog
+分子 #7: THO complex subunit 7 homolog
+分子 #8: Spliceosome RNA helicase DDX39B
+分子 #9: THO complex subunit 4
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
---|---|
![]() | 単粒子再構成法 |
試料の集合状態 | particle |
-
試料調製
濃度 | 0.2 mg/mL |
---|---|
緩衝液 | pH: 7.9 |
凍結 | 凍結剤: ETHANE |
-
電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
---|---|
撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 60.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 0.5 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
-
画像解析
初期モデル | モデルのタイプ: EMDB MAP EMDB ID: |
---|---|
最終 再構成 | 解像度のタイプ: BY AUTHOR / 解像度: 3.9 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 182534 |
初期 角度割当 | タイプ: MAXIMUM LIKELIHOOD |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |