登録情報 データベース : EMDB / ID : EMD-14803 ダウンロードとリンクタイトル Structure of an ALYREF-exon junction complex hexamer マップデータLocally sharpened ALYREF(55-183)-EJC-RNA map 詳細 試料複合体 : Hexameric complex between the TREX subunit ALYREF and the exon junction complexタンパク質・ペプチド : Eukaryotic initiation factor 4A-III, N-terminally processedタンパク質・ペプチド : Protein mago nashi homologタンパク質・ペプチド : RNA-binding protein 8Aタンパク質・ペプチド : Maltose/maltodextrin-binding periplasmic protein,THO complex subunit 4RNA : RNAリガンド : MAGNESIUM IONリガンド : PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER 詳細機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
exon-exon junction subcomplex mago-y14 / transcription export complex / negative regulation of selenocysteine incorporation / regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / cellular response to selenite ion / C5-methylcytidine-containing RNA reader activity / exon-exon junction complex / regulation of translation at postsynapse, modulating synaptic transmission / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / negative regulation of excitatory postsynaptic potential ... exon-exon junction subcomplex mago-y14 / transcription export complex / negative regulation of selenocysteine incorporation / regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / cellular response to selenite ion / C5-methylcytidine-containing RNA reader activity / exon-exon junction complex / regulation of translation at postsynapse, modulating synaptic transmission / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / negative regulation of excitatory postsynaptic potential / Deadenylation of mRNA / regulation of mRNA processing / selenocysteine insertion sequence binding / poly(A) binding / M-decay: degradation of maternal mRNAs by maternally stored factors / Transport of the SLBP independent Mature mRNA / Transport of the SLBP Dependant Mature mRNA / mRNA 3'-end processing / Transport of Mature mRNA Derived from an Intronless Transcript / U2-type catalytic step 1 spliceosome / embryonic cranial skeleton morphogenesis / Transport of Mature mRNA derived from an Intron-Containing Transcript / RNA export from nucleus / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / RNA Polymerase II Transcription Termination / exploration behavior / regulation of alternative mRNA splicing, via spliceosome / associative learning / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / mRNA export from nucleus / ribonucleoprotein complex binding / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / cellular response to brain-derived neurotrophic factor stimulus / RNA stem-loop binding / catalytic step 2 spliceosome / mRNA Splicing - Major Pathway / RNA splicing / positive regulation of translation / response to organic cyclic compound / ISG15 antiviral mechanism / mRNA processing / Regulation of expression of SLITs and ROBOs / mRNA splicing, via spliceosome / osteoblast differentiation / rRNA processing / regulation of translation / outer membrane-bounded periplasmic space / postsynapse / RNA helicase activity / negative regulation of translation / RNA helicase / nuclear speck / mRNA binding / neuronal cell body / glutamatergic synapse / dendrite / nucleolus / ATP hydrolysis activity / RNA binding / extracellular exosome / nucleoplasm / ATP binding / membrane / nucleus / cytosol / cytoplasm 類似検索 - 分子機能 Chromatin target of PRMT1 protein, C-terminal / C-terminal duplication domain of Friend of PRMT1 / C-terminal duplication domain of Friend of PRMT1 / Mago nashi protein / RNA-binding motif protein 8 / RBM8, RNA recognition motif / Mago nashi superfamily / Mago nashi protein / DEAD-box subfamily ATP-dependent helicases signature. / ATP-dependent RNA helicase DEAD-box, conserved site ... Chromatin target of PRMT1 protein, C-terminal / C-terminal duplication domain of Friend of PRMT1 / C-terminal duplication domain of Friend of PRMT1 / Mago nashi protein / RNA-binding motif protein 8 / RBM8, RNA recognition motif / Mago nashi superfamily / Mago nashi protein / DEAD-box subfamily ATP-dependent helicases signature. / ATP-dependent RNA helicase DEAD-box, conserved site / RNA helicase, DEAD-box type, Q motif / DEAD-box RNA helicase Q motif profile. / Maltose/Cyclodextrin ABC transporter, substrate-binding protein / Solute-binding family 1, conserved site / Bacterial extracellular solute-binding proteins, family 1 signature. / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / DEAD/DEAH box helicase / DEAD/DEAH box helicase domain / RNA recognition motif / RNA recognition motif / Eukaryotic RNA Recognition Motif (RRM) profile. / RNA recognition motif domain / Helicase conserved C-terminal domain / RNA-binding domain superfamily / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / Nucleotide-binding alpha-beta plait domain superfamily / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性 Maltose/maltodextrin-binding periplasmic protein / Eukaryotic initiation factor 4A-III / Protein mago nashi homolog / THO complex subunit 4 / RNA-binding protein 8A 類似検索 - 構成要素生物種 Homo sapiens (ヒト)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 2.4 Å 詳細 データ登録者Pacheco-Fiallos FB / Vorlaender MK / Plaschka C 資金援助 European Union, 2件 詳細 詳細を隠すOrganization Grant number 国 European Research Council (ERC) European Union European Molecular Biology Organization (EMBO) 623-2020 European Union
引用ジャーナル : Nature / 年 : 2023タイトル : mRNA recognition and packaging by the human transcription-export complex.著者 : Belén Pacheco-Fiallos / Matthias K Vorländer / Daria Riabov-Bassat / Laura Fin / Francis J O'Reilly / Farja I Ayala / Ulla Schellhaas / Juri Rappsilber / Clemens Plaschka / 要旨 : Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription-export complex (TREX) for nuclear export. However, the ... Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription-export complex (TREX) for nuclear export. However, the mechanisms of mRNP recognition and three-dimensional mRNP organization are poorly understood. Here we report cryo-electron microscopy and tomography structures of reconstituted and endogenous human mRNPs bound to the 2-MDa TREX complex. We show that mRNPs are recognized through multivalent interactions between the TREX subunit ALYREF and mRNP-bound exon junction complexes. Exon junction complexes can multimerize through ALYREF, which suggests a mechanism for mRNP organization. Endogenous mRNPs form compact globules that are coated by multiple TREX complexes. These results reveal how TREX may simultaneously recognize, compact and protect mRNAs to promote their packaging for nuclear export. The organization of mRNP globules provides a framework to understand how mRNP architecture facilitates mRNA biogenesis and export. 履歴 登録 2022年4月21日 - ヘッダ(付随情報) 公開 2023年4月12日 - マップ公開 2023年4月12日 - 更新 2023年5月24日 - 現状 2023年5月24日 処理サイト : PDBe / 状態 : 公開
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