Biotechnology and Biological Sciences Research Council (BBSRC)
BB/H01778X/1
英国
Japan Agency for Medical Research and Development (AMED)
20am0101079
日本
National Institutes of Health/National Cancer Institute (NIH/NCI)
GM31030
米国
引用
ジャーナル: Sci Adv / 年: 2021 タイトル: Molecular mechanism of SbmA, a promiscuous transporter exploited by antimicrobial peptides. 著者: Dmitry Ghilarov / Satomi Inaba-Inoue / Piotr Stepien / Feng Qu / Elizabeth Michalczyk / Zuzanna Pakosz / Norimichi Nomura / Satoshi Ogasawara / Graham Charles Walker / Sylvie Rebuffat / So ...著者: Dmitry Ghilarov / Satomi Inaba-Inoue / Piotr Stepien / Feng Qu / Elizabeth Michalczyk / Zuzanna Pakosz / Norimichi Nomura / Satoshi Ogasawara / Graham Charles Walker / Sylvie Rebuffat / So Iwata / Jonathan Gardiner Heddle / Konstantinos Beis / 要旨: Antibiotic metabolites and antimicrobial peptides mediate competition between bacterial species. Many of them hijack inner and outer membrane proteins to enter cells. Sensitivity of enteric bacteria ...Antibiotic metabolites and antimicrobial peptides mediate competition between bacterial species. Many of them hijack inner and outer membrane proteins to enter cells. Sensitivity of enteric bacteria to multiple peptide antibiotics is controlled by the single inner membrane protein SbmA. To establish the molecular mechanism of peptide transport by SbmA and related BacA, we determined their cryo–electron microscopy structures at 3.2 and 6 Å local resolution, respectively. The structures show a previously unknown fold, defining a new class of secondary transporters named SbmA-like peptide transporters. The core domain includes conserved glutamates, which provide a pathway for proton translocation, powering transport. The structures show an outward-open conformation with a large cavity that can accommodate diverse substrates. We propose a molecular mechanism for antibacterial peptide uptake paving the way for creation of narrow-targeted therapeutics.
EMPIAR-10777 (タイトル: Proton-powered peptide transporter SbmA in lipid nanodisc complexed with Fab S11-1 (SbmA-FabS11-1-MccB17) Data size: 2.5 TB Data #1: SbmA-FabS11-1 complex in lipid nanodisc [micrographs - multiframe])