EMDB-12757: Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slipp...

Basic information

• Entry: Database: EMDB / ID: EMD-12757
• Title: Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot)

Sample

• Complex: Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot
  • Complex: Rabbit 80S ribosome stalled
    • RNA: x 4 types
    • Protein or peptide: x 79 types
  • Complex: SARS-CoV-2 slippery site by a pseudoknot
    • RNA: x 1 types
  • Complex: Rabbit 80S ribosome stalled
    • RNA: x 2 types
  • Complex: Replicase polyprotein 1ab
• Ligand: x 6 types

• Keywords: Frameshift / virus / pseudoknot / RIBOSOME

Function / homology

Function:

Major pathway of rRNA processing in the nucleolus and cytosol / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / ribosomal subunit / regulation of G1 to G0 transition / exit from mitosis / laminin receptor activity / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / optic nerve development / retinal ganglion cell axon guidance / mammalian oogenesis stage / G1 to G0 transition / activation-induced cell death of T cells / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / phagocytic cup / 90S preribosome / TOR signaling / T cell proliferation involved in immune response / protein-RNA complex assembly / erythrocyte development / cellular response to actinomycin D / negative regulation of ubiquitin-dependent protein catabolic process / ribosomal small subunit export from nucleus / translation regulator activity / laminin binding / rough endoplasmic reticulum / gastrulation / MDM2/MDM4 family protein binding / maturation of LSU-rRNA / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / cytosolic ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / rescue of stalled ribosome / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / cellular response to leukemia inhibitory factor / positive regulation of translation / small-subunit processome / protein kinase C binding / positive regulation of apoptotic signaling pathway / positive regulation of protein-containing complex assembly / placenta development / cellular response to gamma radiation / mRNA 5'-UTR binding / transcription coactivator binding / spindle / cytoplasmic ribonucleoprotein granule / modification-dependent protein catabolic process / G1/S transition of mitotic cell cycle / protein tag activity / rRNA processing / ribosomal small subunit biogenesis / antimicrobial humoral immune response mediated by antimicrobial peptide / rhythmic process / positive regulation of canonical Wnt signaling pathway / small ribosomal subunit rRNA binding / protein guanylyltransferase activity / RNA endonuclease activity, producing 3'-phosphomonoesters / mRNA guanylyltransferase activity / 5'-3' RNA helicase activity / ribosome binding / Lyases; Phosphorus-oxygen lyases / glucose homeostasis / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / regulation of translation / ribosomal small subunit assembly / Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / retina development in camera-type eye / Maturation of replicase proteins / virus receptor activity / ISG15-specific peptidase activity / Transcription of SARS-CoV-2 sgRNAs / TRAF3-dependent IRF activation pathway / Translation of Replicase and Assembly of the Replication Transcription Complex / Replication of the SARS-CoV-2 genome / snRNP Assembly / double membrane vesicle viral factory outer membrane / Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters / SARS coronavirus main proteinase / small ribosomal subunit / host cell endoplasmic reticulum-Golgi intermediate compartment / T cell differentiation in thymus / 5S rRNA binding / large ribosomal subunit rRNA binding / 3'-5'-RNA exonuclease activity / 5'-3' DNA helicase activity / symbiont-mediated suppression of host NF-kappaB cascade / host cell endosome / cytosolic small ribosomal subunit

Domain/homology:

60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / 40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ubiquitin-like protein FUBI / Ribosomal protein L30e / Ribosomal L15/L27a, N-terminal / Ribosomal protein L23 / 50S ribosomal protein L10, insertion domain superfamily / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / metallochaperone-like domain / Ribosomal protein L41 / Ribosomal protein L41 / TRASH domain / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein L1, conserved site / Ribosomal protein L1 signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L1 / Ribosomal protein S2, eukaryotic / Small (40S) ribosomal subunit Asc1/RACK1 / S27a-like superfamily / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / 40S Ribosomal protein S10 / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L44e signature. / Plectin/S10, N-terminal / Ribosomal protein L10e, conserved site / Plectin/S10 domain / Ribosomal protein L10e signature. / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein L10e / Ribosomal protein L19, eukaryotic / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein S30 / : / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / : / Ribosomal protein S7e signature. / Ribosomal protein L24e, conserved site / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L24e signature. / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / : / Ribosomal protein L30e signature 1. / Ribosomal protein L6e signature. / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / Ribosomal protein S27e signature. / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein L30e signature 2. / Ribosomal protein S8e, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein S8e signature. / Ribosomal protein L30e, conserved site / Ribosomal protein S19A/S15e / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L36e signature. / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / : / Ribosomal protein L34Ae / Ribosomal protein L34e / Ribosomal protein S6, eukaryotic / Ribosomal S24e conserved site / Ribosomal protein S24e signature.

Component:

Small ribosomal subunit protein eS32 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL29 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein RACK1 / Ubiquitin-ribosomal protein eS31 fusion protein / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL1 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein eS7 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL14 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein eL15 / Small ribosomal subunit protein uS11 / 40S ribosomal protein S24 / Large ribosomal subunit protein uL14 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS25 / Large ribosomal subunit protein eL30 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein eS28 / Small ribosomal subunit protein eS8 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein eS10 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL36 / Small ribosomal subunit protein eS17 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL27 / Large ribosomal subunit protein eL34 / Small ribosomal subunit protein eS27 / Large ribosomal subunit protein eL38 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein eL42 / Large ribosomal subunit protein eL32 / Small ribosomal subunit protein uS14 / Replicase polyprotein 1ab / Large ribosomal subunit protein eL19 / Large ribosomal subunit protein eL37

• Biological species: Oryctolagus cuniculus (rabbit) / Severe acute respiratory syndrome coronavirus 2 / Bos taurus (cattle)
• Method: single particle reconstruction / cryo EM / Resolution: 2.4 Å
• Authors: Bhatt PR / Scaiola A

Funding support

Switzerland, Ireland, 2 items

Organization	Grant number	Country
Swiss National Science Foundation	31003A_182341	Switzerland
Irish Research Council	IRCLA/2019/74	Ireland

• Citation: Journal: Science / Year: 2021; Title: Structural basis of ribosomal frameshifting during translation of the SARS-CoV-2 RNA genome.; Authors: Pramod R Bhatt / Alain Scaiola / Gary Loughran / Marc Leibundgut / Annika Kratzel / Romane Meurs / René Dreos / Kate M O'Connor / Angus McMillan / Jeffrey W Bode / Volker Thiel / David Gatfield / John F Atkins / Nenad Ban / ; Abstract: Programmed ribosomal frameshifting is a key event during translation of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA genome that allows synthesis of the viral RNA-dependent RNA polymerase and downstream proteins. Here, we present the cryo-electron microscopy structure of a translating mammalian ribosome primed for frameshifting on the viral RNA. The viral RNA adopts a pseudoknot structure that lodges at the entry to the ribosomal messenger RNA (mRNA) channel to generate tension in the mRNA and promote frameshifting, whereas the nascent viral polyprotein forms distinct interactions with the ribosomal tunnel. Biochemical experiments validate the structural observations and reveal mechanistic and regulatory features that influence frameshifting efficiency. Finally, we compare compounds previously shown to reduce frameshifting with respect to their ability to inhibit SARS-CoV-2 replication, establishing coronavirus frameshifting as a target for antiviral intervention.

History

Deposition	Apr 14, 2021	-
Header (metadata) release	Jun 2, 2021	-
Map release	Jun 2, 2021	-
Update	Apr 24, 2024	-
Current status	Apr 24, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_12757.map.gz / Format: CCP4 / Size: 669.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.06 Å

Density

Contour Level	By AUTHOR: 5.0 / Movie #1: 5
Minimum - Maximum	-18.107372000000002 - 45.453921999999999
Average (Standard dev.)	-0.0064234477 (±0.9520753)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order Z Y X Origin 0 0 0 Dimensions 560 560 560 Spacing 560 560 560
Cell	A=B=C: 593.6 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.06	1.06	1.06
M x/y/z	560	560	560
origin x/y/z	0.000	0.000	0.000
length x/y/z	593.600	593.600	593.600
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	560	560	560
MAP C/R/S	3	2	1
start NC/NR/NS	0	0	0
NC/NR/NS	560	560	560
D min/max/mean	-18.107	45.454	-0.006

Supplemental data

Mask #1

• File: emd_12757_msk_1.map

Mask #2

• File: emd_12757_msk_2.map

Half map: #2

• File: emd_12757_half_map_1.map

Half map: #1

• File: emd_12757_half_map_2.map

Sample components

Entire : Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slipp...

• Entire: Name: Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot

Components

• Complex: Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot
  • Complex: Rabbit 80S ribosome stalled
    • RNA: 18S rRNA
    • Protein or peptide: 40S ribosomal protein S27
    • Protein or peptide: Ribosomal protein S28
    • Protein or peptide: Ribosomal protein S27a
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: Ribosomal protein eS26
    • Protein or peptide: RACK1
    • Protein or peptide: uS14
    • Protein or peptide: 40S ribosomal protein SA
    • Protein or peptide: 40S ribosomal protein S3a
    • Protein or peptide: Ribosomal protein uS5
    • Protein or peptide: 40S ribosomal protein S3
    • Protein or peptide: Ribosomal protein eS4
    • Protein or peptide: Ribosomal protein S5
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: 40S ribosomal protein S7
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: Ribosomal protein S9 (Predicted)
    • Protein or peptide: eS10
    • Protein or peptide: 40S ribosomal protein S11
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: uS15
    • Protein or peptide: 40S ribosomal protein uS11
    • Protein or peptide: 40S ribosomal protein uS19
    • Protein or peptide: uS9
    • Protein or peptide: 40S ribosomal protein eS17
    • Protein or peptide: 40S ribosomal protein S18
    • Protein or peptide: Ribosomal protein eS19
    • Protein or peptide: 40S ribosomal protein uS10
    • Protein or peptide: Ribosomal protein eS21
    • Protein or peptide: Ribosomal protein S15a
    • Protein or peptide: 40S ribosomal protein S23
    • Protein or peptide: 40S ribosomal protein S24
    • Protein or peptide: 40S ribosomal protein S25
    • Protein or peptide: 60s ribosomal protein l41
    • RNA: 28S rRNA
    • RNA: 5S
    • RNA: 5.8S rRNA
    • Protein or peptide: Ribosomal protein uL2
    • Protein or peptide: Ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L4
    • Protein or peptide: Ribosomal_L18_c domain-containing protein
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: Ribosomal Protein uL30
    • Protein or peptide: Ribosomal protein eL8
    • Protein or peptide: 60S ribosomal protein L9
    • Protein or peptide: 60S ribosomal protein L10
    • Protein or peptide: Ribosomal protein L11
    • Protein or peptide: Replicase polyprotein 1ab
    • Protein or peptide: Ribosomal protein eL13
    • Protein or peptide: Ribosomal protein L14
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: Ribosomal protein uL13
    • Protein or peptide: uL22
    • Protein or peptide: Ribosomal Protein eL18
    • Protein or peptide: 60S ribosomal protein L19
    • Protein or peptide: Ribosomal protein eL20
    • Protein or peptide: eL21
    • Protein or peptide: Ribosomal protein eL22
    • Protein or peptide: Ribosomal protein L23
    • Protein or peptide: eL24
    • Protein or peptide: uL23
    • Protein or peptide: Ribosomal protein L26
    • Protein or peptide: 60S ribosomal protein L27
    • Protein or peptide: 60S ribosomal protein L27a
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: eL30
    • Protein or peptide: eL31
    • Protein or peptide: eL32
    • Protein or peptide: eL33
    • Protein or peptide: 60S ribosomal protein L34
    • Protein or peptide: uL29
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: eL38
    • Protein or peptide: eL39
    • Protein or peptide: 60S ribosomal protein L40
    • Protein or peptide: eL42
    • Protein or peptide: eL43
    • Protein or peptide: Ribosomal protein eL28
    • Protein or peptide: 60S acidic ribosomal protein P0
    • Protein or peptide: Ribosomal protein L12
    • Protein or peptide: Ribosomal protein uL1
  • Complex: SARS-CoV-2 slippery site by a pseudoknot
    • RNA: mRNA containing SARS-CoV-2 sequence
  • Complex: Rabbit 80S ribosome stalled
    • RNA: E-site tRNA
    • RNA: P-site Phe-tRNA(Phe)
  • Complex: Replicase polyprotein 1ab
• Ligand: SPERMIDINE
• Ligand: SPERMINE
• Ligand: MAGNESIUM ION
• Ligand: UNKNOWN ATOM OR ION
• Ligand: ZINC ION
• Ligand: water

Supramolecule #1: Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slipp...

• Supramolecule: Name: Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#86

Supramolecule #2: Rabbit 80S ribosome stalled

• Supramolecule: Name: Rabbit 80S ribosome stalled / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#8, #12-#86
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)

Supramolecule #3: SARS-CoV-2 slippery site by a pseudoknot

• Supramolecule: Name: SARS-CoV-2 slippery site by a pseudoknot / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #9
• Source (natural): Organism: Severe acute respiratory syndrome coronavirus 2

Supramolecule #4: Rabbit 80S ribosome stalled

• Supramolecule: Name: Rabbit 80S ribosome stalled / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #10-#11
• Source (natural): Organism: Bos taurus (cattle)

Supramolecule #5: Replicase polyprotein 1ab

• Supramolecule: Name: Replicase polyprotein 1ab / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #52
• Source (natural): Organism: Severe acute respiratory syndrome coronavirus 2

Macromolecule #1: 18S rRNA

• Macromolecule: Name: 18S rRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 603.92825 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAU(A2M)UGC UUG(PSU)C(PSU)CAAA GAUUAAGCCA UGCAUGUCUA AGUACGCA C GGCCGGUACA GUGAAACUGC GAA(PSU)GGCUC(A2M) UUAAA(PSU)CAG(PSU) UAUGGU(OMU)CC(PSU) U (OMU)GGUCGCU CGCUCCUCUC CUACUUGGAU AACUGUGGUA (A2M)UUCUAG(A2M)GC UAA(OMU)A(OMC)AUGC CG ACGGGCG CUGACCCCCU UCGCGGGGGG GA(PSU)GCGUGCA (PSU)UUAUCAGAU CAAAACCAAC CCGGUCAGCC UCCCC CCGG CCCCGGCCGG GGGGGUGGGC GCCGGCGGCU UUGGUGACUC UAGAUAACCU CGGGCCGAUC GCACGCCCUC CGUGGC GGC GACGACCCAU UCGAACGUC(OMU) GCCCUAUCAA CUUUCGAUGG UAGUCGCCGU GCCUACCAUG GUGACCACGG G (PSU)GACGGGG AAUCAGGGUU CGAU(OMU)CCGGA GA(OMG)GGAGCCU GAGAAACGGC UACCACAU(OMC)C AAGG (A2M)AGGC AGCAGGCGCG C(A2M)AAUUACCC ACUCCCGACC CGGGGA(OMG)GU(A2M) GUGA(OMC)GAAAA AUAAC AAUA CAGGACUCUU UCGAGGCCCU GUAAUUGGAA UGAGUCCACU (PSU)UAA(A2M)UCCUU UAACGAGG(A2M)U CCAU UGGAG (OMG)GCAAGUC(PSU)G GUGCCAGCAG CCGCGG(OMU)AAU UCCAGCUCCA AUA(OMG)CGUA(PSU)A (PSU) UAAAGUUG CUGCAGUU(A2M)A AAAGCUCGUA G(PSU)U(OMG)GA(PSU)CUU GGGAGCGGGC GGGCGGUCCG CCGCGA GGC GAGCCACCGC CCGUCCCCGC CCCUUGCCUC UCGGCGCCCC CUCGAUGCUC UUAGCUGAGU GUCCCGCGGG GCCCGAA GC GUU(PSU)ACUUUG AAAAAA(PSU)(PSU)AG AGUG(PSU)UCAAA GCAGGCCCGA GCCGCCUGGA UACCGCAGCU AG GAA(PSU)AA(PSU) (OMG)GAAUAGGAC CGCGGUUCUA UUUUGUUGGU UUUCGGAACU GAGGCCAUGA UUAAGAGGGA C GGCCGGGG GCAUUCGUAU UGCGCCGCUA GAGGUGAAAU (PSU)CUUGGACCG GCGCAAGACG GACCAGAGCG AAAGCAUU (PSU) GCCAAGAAUG UUUUCAUUAA UCAAGA(A2M)CGA AAGUCGGAGG (PSU)(PSU)CGAAGACG A(PSU)CAGAUAC CGUCGUAGUU CCGACCA(PSU)AA ACGAUGCCGA CUGGCGAUGC GGCGGCGUUA UUCCCAUGAC CCGCCGGGCA GCUUCC GGG AAACCAAAGU CUUUGGGUUC CGGGGGGAGU A(PSU)GG(PSU)UGCAA AGCUGAAACU UAAAGGAAUU GACGGAAGG GCACCACCAG GAGUGGAGCC (PSU)GCGGC(PSU)UAA UU(PSU)GAC(B8N)CAA CACGGGAAAC CUCACCCGGC CCGG ACACG GACAGGA(OMU)UG ACAGAUUGAU AGCUCUUUCU CGAUUCCGUG GGUGG(OMU)G(OMG)UG CAUGGC(4AC)GU UCUUAGU(PSU)GG UGGAGCGAUU UGUCUGG(PSU)UA AUUCCGAUAA CGA(A2M)CGAGAC U(OMC)UGGCAUGC UAAC UAGUU ACGCGACCCC CGAGCGGUCG GCGUCCCCCA ACU(OMU)CU(PSU)A(OMG)A GGGACAAGUG GCGUUCAGCC ACC CGAGAU UGAGCAAUAA CA(OMG)GUCUGUG AUGCCCUUAG AUGUCCGGGG CUGCACGCGC GCUACACUGA CUGGCUCAGC GUGUGCCUA CCCUACGCCG GCAGGCGCGG GUAACCCGUU GAACCCCAUU CGUGAUGGGG AUCGGGGAUU GCAAUUAU (PSU) CCCCAUGAAC GAG(G7M)AAU(PSU)CC CAGUAAGUGC GGGUCAUAAG CUUGCGUUGA UU(A2M)AGUCCCU GCC CUU(PSU)GU ACACACCG(OMC)C CGUCGCUACU ACCGAUUGGA UGGUUUAGUG AGGCCCUCGG AUCGGCCCCG CCGGGG UCG GCCCACGGCC CUGGCGGAGC GCUGAGAAGA CGGUCGAACU (OMU)GACUAUCUA GAGGAAGUAA AAGUCGUA(6MZ) CAAGGUUUC(4AC) GUAGGUG(MA6)(MA6)C CUGCGGAAGG AUCAUUA

GENBANK: GENBANK: GBCT01000564.1

Macromolecule #9: mRNA containing SARS-CoV-2 sequence

• Macromolecule: Name: mRNA containing SARS-CoV-2 sequence / type: rna / ID: 9 / Number of copies: 1
• Source (natural): Organism: Severe acute respiratory syndrome coronavirus 2
• Molecular weight: Theoretical: 70.6015 KDa

Sequence

String:

CAACUUGUGC UAAUGACCCU GUGGGUUUUA CACUUAAAAA CACAGUCUGU ACCGUCUGCG GUAUGUGGAA AGGUUAUGGC UGUAGUUGU GAUCAACUCC GCGAACCCAU GCUUCAGUCA GCUGAUGCAC AAUCGUUUUU AUAAGGGUUU GCGGUGUAAG U GCAGCCCG UCUUACACCG UGCGGCACAG GCACUAGUAC UGAUGUCGUA UACAGGGCUU UU

GENBANK: GENBANK: NC_045512.2

Macromolecule #10: E-site tRNA

• Macromolecule: Name: E-site tRNA / type: rna / ID: 10 / Number of copies: 1
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 15.209544 KDa

Sequence

String:

(N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N) (N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N) (N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)CCA

Macromolecule #11: P-site Phe-tRNA(Phe)

• Macromolecule: Name: P-site Phe-tRNA(Phe) / type: rna / ID: 11 / Number of copies: 1
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 24.889115 KDa

Sequence

String:

GCCGAAAUA(2MG) CUC(1MA)G(H2U)(H2U)GGG AGAGC(M2G)(PSU)(PSU)AG A(OMC)U(OMG)AA(YYG)A (PSU) CUAAAG(G7M)UC(5MC) CUGG(5MU)(PSU)CG(1MA)U CCCGGGUUUC GGCACCA

Macromolecule #39: 28S rRNA

• Macromolecule: Name: 28S rRNA / type: rna / ID: 39 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 1.5575195 MDa

Sequence

String:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGUGGG GCGCGGGAAA UGUGGCGUAC G GAAGACCC ACUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGUA AAC UCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA G (A2M)G(A2M)GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUUC AACCC GGCGGCGUGG UCCGGCCGUG CCGGCGGUCU CGGCGGAUCU UUCCCGCUCC CCGUUCCUCC CGACCCCUCC ACCCG CUCC CCCUCCCCCG CCGGUCUUCU CCCUUCACGG GGGGUGGGUC GGCGGGUGCG GGGGUGGGCG GGCGGGGCCG GGGGUG GGG UCGGCGGGGG ACCGCCCCCC GGCCGGCGAC CGGCCGCCGC CGGGCGCAUU UCCACCGCGG CGGUGCGCCG CGACCGG CU CCGGGACGGC UGGGAAGGCC CCGGCGGGGA AGGUGGCCCG GGGGGACGCC UCGCCGCCGU CGUCCGCGGC GGCGGCGG G CGCGACCCCU CCCCGGGUGU UACAGCCCCG CCCCGGCAGC AGCAGCUCGC CGAAUCCCGG GGCCGAGGGA GCGAGACCU GUGUCGCCGC GCUCUCCCCC CUCCCGGCGC UCCCCCCCGC GGGGGUCCCC CGCGAGGGGG CUCCCCCCGC GGGGGCGCGC CGGUGUCGC CGCCCUCGCG GGCGCGGCAC GGGGGGGCCG GGCCGCCCCU CCCACGGCGC GACCGCUCUC CCGGCCCCCG C CGCCGCCU CUUCGGGCGU GCGGCGGGGG UGGGGCGGAC UGUCCCCAGU GCGCCCCGGG CGUGUCACGC CGUCGGGCUC GG GGGAGAA GUCGGCCACG GCGCGUCGCG CGCCUCCGCG UCCCCCCCUC GCCGUCUCCG GGCGGCGGGG GGGGGUUUCG GCG CGGUGU UCUCGGCGCG CGCGCGCGAG CGAGCGCACG GGGUCGGCGG CGAUGUCGGC UACCCACCCG ACCC(OMG)UCUUG (1MA)AAC(A2M)CGGA CCAAGGAGU(OMC) UAACACGUGC GCGAGUCGGG GGCUCGCACG AAAGCCGCCG UGGCGCAAU GAAGGUGAAG GCCGGCGGCG GCUUCCCGCC GUCGUCGGCC GAGGUGGGAU CCCGAGGCCC UUCCAGUCCG CCGAGGGCGC ACCACCGGC CCGUCUCGCC CGCCGCGCCG GGGAGGUGGA GCACGAGCGC ACGUGUUAGG ACCC(OMG)A(A2M)AGA UGG UGA(A2M)C(PSU) AUGCCUGGGC AGGGCGAAGC CAGAGGAAAC UCUGGUGGAG GUCCG(PSU)AGCG GUCCUGACGU GC AAAUCGG UCGUCCGACC UGGGUAUAG(OMG) GGCGAAAGAC UAAUCGAACC AUCUAGUAGC UGGUUCCCUC CGAAGUUUC CC(PSU)CAGGA(PSU)A GCUGGCGCUC UCGCAACCCC GCAGUUUUAU CCGGUAAAGC GAA(PSU)GAUUAG AGGUCUUG G GGCCGAAACG AUCUCAACC(PSU) A(PSU)(PSU)CUCAAAC UU(PSU)AAAUGGG UAAGAAGCCC GGCUCGCUGG CGU GGAGCC GGGCGUGGAA UGCGAGUGCC UAGUGGGCCA C(PSU)U(PSU)UGGUAA GC(A2M)GAACUGG CG(OMC)UGCGG G AUGAACCGAA CGCCGGGUUA AGGCGCCCGA UGCCGACGCU CAUCAGACCC CAGAAAAGGU GUUGGUUGAU AUAGACAGC AGGACGGUGG CCAUGGAAGU CGGAAUCCGC UAAGGAGUGU GUAACAACUC ACCUGCCGAA UCAACUAGCC CUGAAAAUGG AUGGCGCUG GAGCGUCGGG CCCAUACCCG GCCGUCGCUG GCAGUCGGCA GAGCGAGAAA GAGAGUGGAG CGCGCCGCGG G ACUCCCCC CGCGGGGGUC CCCGGCCACC CCCCCCCCGC GGACGCUACG CCGCGACGAG UAGGAGGGCC GCUGCGGUGA GC CUUGAAG CCUAGGGCGC GGGCCCGGGU GGAGCCGCCG CAGGUGCAGA U(OMC)UUGGUGGU AGU(A2M)(OMG)(OMC)A AA UAUUCAAACG AGAACUUUGA AGGCCGAAGU GG(A2M)GAAGGGU UCCAUG(OMU)GAA CAG(OMC)A(OMG)UUGA AC AUGGGUC AGUCGGUCCU GAGAGAUGGG CGAGCGCCGU UCCGAAGGGA CGGGCGAUGG CCUCCGUUGC CCUCAGCCGA (PSU)CGAAAGGG AGUCGGGUUC AGAUCCCCGA AUCCGGAGUG GCGGAGAUGG GCGCCGCGAG GCGUCCAGUG CGGUAAC GC GACCGAUCCC GGAGAAGCCG GCGGGAGCCC CGGGGAGAGU UCUCUU(PSU)UCU UUGUGAAGGG CAGGGCGCCC UGGA AUGGG UUCGCCCCGA GAGAGGGGCC CGUGCCUUGG AAAGCGUCGC GGUUCCGGCG GCGUCCGGUG AGCUCUCGCU GGCCC UUGA AAAUCCGGGG GAGAGGGUGU AAAUCUCGCG CCGGGCCGUA CCC(A2M)UAUCCG CAGCAGGUCU (OMC)CAAGGUG A AC(A2M)GCCUCUG G(OMC)AUGUUGGA ACAA(OMU)GUAGG UAAGGGAAGU CGGCAAGC(OMC)G GAUCCGUAAC UUC (OMG)GGAUA AGGAUUGGCU CUAAGGGCUG GGUCGGUCGG GCUGGGGCGC GAAGCGGGGC UGGGCGCGCG CCGCGGCUGG ACGAGGCGC CGCCGCCCCC CCCACGCCCG GGGCGCCCUC CCCCGGCCGG GUCCGCUCCC UCGCGCGCGC GUGCGCCGUG G CGCGCGCC GCGCGCGCGG CCGAGCCCCG UCUCCCUCCC CCCCUCUCCC CUCCCGGGGC GGGGUGCGGG GAGGGGCGGG CG GGGCCGU CGCUCGCCGC GCGUGCGCGU CGCUGCGCGU GCGCCGCGCG GGGGGUCCGG CGGGCCUCCG GACGGGGGCU CCG GGCACC CGGGCACCCG GGGGGCCGGC GGCGGCGGCG ACUCUGGACG CGAGCCGGGC CCUUCCCGUG GAUCGCCCCA GCUG CGGCG GGCGUCGCGG CCGCCCUCGG GGAGCCCGGC GGGCGCCGCG UGCGCGCGCG UGCGCGCCCG GGGUCGGGGG GGUGC GAGG CAGGGGAGGC CCUCUCUCCU CCUCCCCCAC CCCGGCGCCG CGGCGCCGCG CGUGCGCGCG CGGUCUCCCC CCGCCG GGU CCGCCCCCGG GGCCGCGGUU CCGCGCGGCG CCUCGCCUCG GCCGGCGCCU AGCAGCCGAC UUAGAACUGG UGCGGAC CA GG(OMG)GAAUCCG AC(PSU)G(PSU)UUAAU UAAAACAAAG CAUCGCGAAG GCCCGCGGCG GGUGUUGACG CGAUGU GAU U(PSU)CUGCC(OMC)AG UGCUCUGAAU G(PSU)CA(A2M)AGUGA (A2M)GAAAU(PSU)CAA (PSU)GAAGCGCG G(OMG)UAAACGGC GGGAG(PSU)A(A2M)C(PSU) A(PSU)GAC(PSU)C(PSU)CU UAAGGUAGC(5MC) AA(A2M)U GCCUC (OMG)UCAUCUAAU UAGUGA(OMC)GCG CAUGAA(UY1)GGA (PSU)GA(A2M)CGAG(A2M)U UCCCACUGU (OMC)CC(PSU)ACCUAC (PSU)A(PSU)CCAGCGA AACCAC(A2M)G(OMC)C AAGGGAACGG GCU(PSU)GG(OMC)G G AAUCAGCGG(OMG) GAAAGAAGAC CCUGUUGAGC (PSU)UGAC(OMU)CUAG UCUGGCACGG UGAA(OMG)AGACA UGA GAGGUG UAGAAUAAGU GGGAGGCCCC CGGCGCCCUC CCGCCCCCGC GAGGGGGCGG GGCGGGGUCC GCCGGCCUUG CGGG CCGCC GGUGAAAUAC CACUACUCUU AUCGUUUUUU CACUGACCCG GUGAGGCGGG GGGGCGAGCC CCGAGGGGCU CUCGC UUCU GGCGCCAAGC GCCGGGCCCC GUCGGUCCGC GCGGGCCGGC GGCGGCCGGG CGCGACCCGC UCCGGGGACA GUGCCA GGU GGGGAGUUUG ACUG(OMG)GGCGG UACACCUGUC AAACGGUA(6MZ)C GCAGG(OMU)(OMG)UCC UAAGGCGAGC U CAGGGAGG ACAGAAACCU CCCGUGGAGC AGAAGGGCAA AAGCUCGCUU GA(PSU)CU(PSU)GA(PSU)U UUCAG(OMU) ACG AA(PSU)ACAGACC GUGAAAGCGG GGCCUCACGA UCCUUCUGAG CUU(PSU)UGGGUU U(PSU)AAGCAGGA (OMG) GUGUCAGA AAAGUUACCA CAG(OMG)GAUAAC UGGC(PSU)UGUGG CGGCCAAGCG U(PSU)CA(PSU)AGCGA CG (PSU)CGCUUU UUGA(PSU)CCUU(5MC) GAUGUCGG(OMC)(PSU) CUUCCUAUCA UUG(PSU)GAAGCA GAAUUCACC AAGCGUU(OMG)GA U(OMU)(OMG)(PSU)UCACCC ACUAAUAGGG AACG(PSU)G(A2M)GCU GGG(UR3)U(PSU)AG A (OMC)CGUCGUGAG ACAGGU(PSU)AGU UUUACCCUAC UGAUG(A2M)UGUG (PSU)UG(PSU)UGCCAU GGUA(A2M) UCCU GCUCAGUACG AGAGGAACCG CAG(OMG)U(OMU)CA(OMG)A CAU(PSU)UGGUGU A(PSU)(OMG)UGCUUGG CU GAGGAGC CAAUGGGGCG AAGCUACCA(PSU) CUGUGGGAUU AUGAC(PSU)GAAC GCCUCUAAGU CAGAAUCCCG CCCAG GCGG AACGAUACGG CAGCGCCGAA GGAGCCUCGG UUGGCCUCGG AUAGCCGGUC CCCCGCCGUC CCCGCCGGCG GCCCGC GUC GUGCGUCCGC CUCGGCGGCG UGCGGCGCGC CCCCCGCCGC GCGUCGGGAC CGGGGUCCGG UGCGGAGAGC CCUUCGU CC CGGGAAAUGG GGCGCGGCCG GAAAGGGGGC CGCCCUCUCG CCCGUCACGC ACCGCACGUU CGUGGGGAAC CUGGCGCU A AACCA(PSU)UCGU AGACGACCUG CUUCUGGGUC GGGG(PSU)UUCGU ACG(PSU)AGCAGA GCAGCUCCCU CGCUGCG AU CUAUUGAAAG UCAGCCCUCG ACACAAGGGU UUGU

GENBANK: GENBANK: GBCN01009604.1

Macromolecule #40: 5S

• Macromolecule: Name: 5S / type: rna / ID: 40 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 38.851871 KDa

Sequence

String:

(GTP)UCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGU ACU UGGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU U

GENBANK: GENBANK: GBCM01014045.1

Macromolecule #41: 5.8S rRNA

• Macromolecule: Name: 5.8S rRNA / type: rna / ID: 41 / Number of copies: 1
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 50.809047 KDa

Sequence

String:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGC(PSU)GCGAG AAUUAAUG(PSU)G AA UU(OMG)CAGG ACACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGA GCGUCGCUUG

GENBANK: GENBANK: GBCN01009604.1

Macromolecule #2: 40S ribosomal protein S27

• Macromolecule: Name: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 9.480186 KDa

Sequence

String:

MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

UniProtKB: Small ribosomal subunit protein eS27

Macromolecule #3: Ribosomal protein S28

• Macromolecule: Name: Ribosomal protein S28 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 7.855052 KDa

Sequence

String:

MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

UniProtKB: Small ribosomal subunit protein eS28

Macromolecule #4: Ribosomal protein S27a

• Macromolecule: Name: Ribosomal protein S27a / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 18.004041 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKSYTTPK KNKHKRKKVK LAVLKYYKVD ENGKISRLRR ECPSDECGAG VFMASHFDRH YCGKCCLTYC FNKPEDK

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

Macromolecule #5: 40S ribosomal protein S30

• Macromolecule: Name: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.498884 KDa

Sequence

String:

MQLFVRAQEL HTLEVTGRET VAQIKAHVAS LEGIAPEDQV VLLAGTPLED EATLGQCGVE ALSTLEVAGR MLGGKVHGSL ARVGKVRGQ TLKVAKQEKK KKRTGRAKRR MQYNRRFVNV VPTFGKKKGP NANS

UniProtKB: Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein

Macromolecule #6: Ribosomal protein eS26

• Macromolecule: Name: Ribosomal protein eS26 / type: protein_or_peptide / ID: 6 / Details: Depositor reference: GB XP_008254857.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.047532 KDa

Sequence

String:

MTKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEASVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAGAAPR PPPKPM

Macromolecule #7: RACK1

• Macromolecule: Name: RACK1 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 35.115652 KDa

Sequence

String:

MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #8: uS14

• Macromolecule: Name: uS14 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 6.690821 KDa

Sequence

String:

MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #12: 40S ribosomal protein SA

• Macromolecule: Name: 40S ribosomal protein SA / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 32.970023 KDa

Sequence

String:

M(SAC)GALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSV ISS RNTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRY VD IAIPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAP E FTATQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGTTTEWS

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #13: 40S ribosomal protein S3a

• Macromolecule: Name: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 30.002061 KDa

Sequence

String:

MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGSSS GK ATGDETG AKVERADGYE PPVQESV

UniProtKB: Small ribosomal subunit protein eS1

Macromolecule #14: Ribosomal protein uS5

• Macromolecule: Name: Ribosomal protein uS5 / type: protein_or_peptide / ID: 14 / Details: Sequence from GeneBank entry GBAX01005410.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 31.285426 KDa

Sequence

String:

MADDAGAAGG PGGPGGPGMG GRGGFRGGFG SGIRGRGRGR GRGRGRGRGA RGGKAEDKEW LPVTKLGRLV KDMKIKSLEE IYLFSLPIK ESEIIDFFLG AALKDEVLKI MPVQKQTRAG QRTRFKAFVA IGDYNGHVGL GVKCSKEVAT AIRGAIILAK L SIVPVRRG YWGNKIGKPH TVPCKVTGRC GSVLVRLIPA PRGTGIVSAP VPKKLLMMAG IDDCYTSARG CTATLGNFAK AT FDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT

Macromolecule #15: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 31.146607 KDa

Sequence

String:

MSARRRRRRA AFRRRAEPFI PISVREPLPF LSAARGGKMA VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG V LRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPSGKIGP KK PLPDHVS IVEPKDEILP TTPISEQKGG KPEPPAMPQP VPTA

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #16: Ribosomal protein eS4

• Macromolecule: Name: Ribosomal protein eS4 / type: protein_or_peptide / ID: 16 / Details: Sequence from GeneBank entry XP_002720153.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 29.654869 KDa

Sequence

String:

MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

Macromolecule #17: Ribosomal protein S5

• Macromolecule: Name: Ribosomal protein S5 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 22.913453 KDa

Sequence

String:

MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #18: 40S ribosomal protein S6

• Macromolecule: Name: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 28.751906 KDa

Sequence

String:

MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

UniProtKB: Small ribosomal subunit protein eS6

Macromolecule #19: 40S ribosomal protein S7

• Macromolecule: Name: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 47.356172 KDa

Sequence

String:

EPITNRVFQA LTSSDFKQQD GGSFSLSRTH LSGFRSRPRR TTIASAATRP PPIPSRGGSA PTGTPEGAGA SRSSRTVPRL PPQPSGPPR APRHPGFQRA LPSLIRPEPG VRAPLLASAG TRFKRPASQA VKALSARGLL QPQVQVGPRA NRVPQRREPR Q HLSGHAPG ASEGGSASPT YWSLGITSWD ALGFPLRLGG SSGFRSSVAL FRPLSFRRGD LPRSREIWAS SSAGAQPGEA MF SSSAKIV KPNGEKPDEF ESGISQALLE LEMNSDLKAQ LRELNITAAK EIEVGGGRKA IIIFVPVPQL KSFQKIQVRL VRE LEKKFS GKHVVFIAQR RILPKPTRKS RTKNKQKRPR SRTLTAVHDA ILEDLVFPSE IVGKRIRVKL DGSRLIKVHL DKAQ QNNVE HKVETFSGVY KKLTGKDVNF EFPEFQL

UniProtKB: Small ribosomal subunit protein eS7

Macromolecule #20: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.263387 KDa

Sequence

String:

MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

UniProtKB: Small ribosomal subunit protein eS8

Macromolecule #21: Ribosomal protein S9 (Predicted)

• Macromolecule: Name: Ribosomal protein S9 (Predicted) / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 22.641564 KDa

Sequence

String:

MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #22: eS10

• Macromolecule: Name: eS10 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 18.933846 KDa

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGL EGERPARLTR GEADRDTYRR SAVPPGADKK AEAGAGSATE FQFRGGFGRG R GQPPQ

UniProtKB: Small ribosomal subunit protein eS10

Macromolecule #23: 40S ribosomal protein S11

• Macromolecule: Name: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 18.468826 KDa

Sequence

String:

MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #24: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.538987 KDa

Sequence

String:

MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

UniProtKB: Small ribosomal subunit protein eS12

Macromolecule #25: uS15

• Macromolecule: Name: uS15 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.259389 KDa

Sequence

String:

MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #26: 40S ribosomal protein uS11

• Macromolecule: Name: 40S ribosomal protein uS11 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 16.302772 KDa

Sequence

String:

MAPRKGKEKK EEQVISLGPQ VAEGENVFGV CHIFASFNDT FVHVTDLSGK ETICRVTGGM KVKADRDESS PYAAMLAAQD VAQRCKELG ITALHIKLRA TGGNRTKTPG PGAQSALRAL ARSGMKIGRI EDVTPIPSDS TRRKGGRRGR RL

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #27: 40S ribosomal protein uS19

• Macromolecule: Name: 40S ribosomal protein uS19 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.049182 KDa

Sequence

String:

MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLSRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #28: uS9

• Macromolecule: Name: uS9 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 19.213465 KDa

Sequence

String:

SARVAPRFPF PVVAPRGEDR HSSAAAMPSK GPLQSVQVFG RKKTATAVAH CKRGNGLIKV NGRPLEMIEP RTLQYKLLEP VLLLGKERF AGVDIRVRVK GGGHVAQIYA IRQSISKALV AYYQKYVDEA SKKEIKDILI QYDRTLLVAD PRRCESKKFG G PGARARYQ KSYR

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #29: 40S ribosomal protein eS17

• Macromolecule: Name: 40S ribosomal protein eS17 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.552119 KDa

Sequence

String:

MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGAV

UniProtKB: Small ribosomal subunit protein eS17

Macromolecule #30: 40S ribosomal protein S18

• Macromolecule: Name: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.801814 KDa

Sequence

String:

M(SAC)LVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQ YKI PDWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #31: Ribosomal protein eS19

• Macromolecule: Name: Ribosomal protein eS19 / type: protein_or_peptide / ID: 31 / Details: Sequence from GeneBank entry DN883431.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 16.104579 KDa

Sequence

String:

MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYL(NMM)GGA GVGSMT KIY GGRQRNGVMP SHFSRGSKSV ARRVLQALEG LKMVEKDQDG GRKLTPQGQR DLDRIAGQVA AANKKH

Macromolecule #32: 40S ribosomal protein uS10

• Macromolecule: Name: 40S ribosomal protein uS10 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.398763 KDa

Sequence

String:

MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #33: Ribosomal protein eS21

• Macromolecule: Name: Ribosomal protein eS21 / type: protein_or_peptide / ID: 33 / Details: Sequence from UniProt entry DN885342.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 9.181374 KDa

Sequence

String:

(AME)QNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKSD GIV SQNF

Macromolecule #34: Ribosomal protein S15a

• Macromolecule: Name: Ribosomal protein S15a / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.865555 KDa

Sequence

String:

MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #35: 40S ribosomal protein S23

• Macromolecule: Name: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.860666 KDa

Sequence

String:

MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK Q(HY3)NSAIRKCV RVQLIK NGK KITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #36: 40S ribosomal protein S24

• Macromolecule: Name: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.107924 KDa

Sequence

String:

MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK K

UniProtKB: 40S ribosomal protein S24

Macromolecule #37: 40S ribosomal protein S25

• Macromolecule: Name: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.645028 KDa

Sequence

String:

PPKDDKKKKD AGKSAKKDKD PVNKSGGKAK KKKWSKGKVR DKLNNLVLFD KATYDKLCKE VPNYKLITPA VVSERLKIRG SLARAALQE LLSKGLIKLV SKHRAQVIYT RNTKGGDAPA AGEDA

UniProtKB: Small ribosomal subunit protein eS25

Macromolecule #38: 60s ribosomal protein l41

• Macromolecule: Name: 60s ribosomal protein l41 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 3.473451 KDa

Sequence

String:

MRAKWRKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

Macromolecule #42: Ribosomal protein uL2

• Macromolecule: Name: Ribosomal protein uL2 / type: protein_or_peptide / ID: 42 / Details: Sequence from GeneBank entry XP_002723515.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 28.103855 KDa

Sequence

String:

MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV VAGGGRIDKP ILKAGRAYHK YKAKRNCWPR VRGVAMNPVE HPFGGGN(V5N)QH IGKPSTIRRD APAGRKVG L IAARRTGRLR GTKTVQEKEN

Macromolecule #43: Ribosomal protein L3

• Macromolecule: Name: Ribosomal protein L3 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 46.120996 KDa

Sequence

String:

MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DAGKRQLDKD FSSMKKYCQV I RVLAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVS QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKT(HIC)RG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINP LGGFVHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTVEEKK AFMGPLKKDR IAKEEGA

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #44: 60S ribosomal protein L4

• Macromolecule: Name: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 46.430895 KDa

Sequence

String:

M(AYA)CARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARI PRV RGGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVED KV EGYKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC VIYNEDNGIV KAFRNIPGIT LLNVTKLN I LKLAPGGHVG RFCIWTESAF RKLDDLYGTW RKAASLKSNY NLPMHKMLNT DLSRILKSPE IQRALRAPRK KIHRRVLKK NPLKNLRIML KLNPYAKTMR RNTILRQARN HKLRVERAAA ALAAKSDPKE APAKKKPVVG KKKKPVVGRK AAAAKKPAAD KKAADKRAG PEDKKPAA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #45: Ribosomal_L18_c domain-containing protein

• Macromolecule: Name: Ribosomal_L18_c domain-containing protein / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 34.523863 KDa

Sequence

String:

M(AAC)FVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVC AAY AHELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTG NK VFGALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NNVTPDMM E EMYKKAHAAI RENPVYEKKP KREVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #46: 60S ribosomal protein L6

• Macromolecule: Name: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 33.028336 KDa

Sequence

String:

MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGKKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL KQLSSGLLLV TGPLSLNRVP LRRTHQKFVI ATSTKIDISG VKIPKHLTDA YFKKKKLRKP RHQEGEIFDT EK EKYEITE QRKVDQKAVD SQILPKIKAV PQLQGYLRSV FALTNGVYPH KLVF

UniProtKB: Large ribosomal subunit protein eL6

Macromolecule #47: Ribosomal Protein uL30

• Macromolecule: Name: Ribosomal Protein uL30 / type: protein_or_peptide / ID: 47 / Details: Sequence from GeneBank entry XP_002710681.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 29.201836 KDa

Sequence

String:

MEGAEEKKKV PAVPETLKKK RRNFAELKIK RLRKKFAQKM LRKARRKLIY EKAKHYHKEY RQMYRTEIRM ARMARKAGNF YVPAEPKLA FVIRIRGING VSPKVRKVLQ LLRLRQIFNG TFVKLNKASI NMLRIVEPYI AWGYPNLKSV NELIYKRGYG K INKKRIAL TDNTLIARSL GKYNIICMED LIHEIYTVGK HFKEANNFLW PFKLSSPRGG MKKKTTHFVE GGDAGNREDQ IN RLIRRMN

Macromolecule #48: Ribosomal protein eL8

• Macromolecule: Name: Ribosomal protein eL8 / type: protein_or_peptide / ID: 48 / Details: Sequence from GeneBank entry GBCD01000651.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 30.061785 KDa

Sequence

String:

MPKGKKAKGK KVAPAPAVVK KQEAKKVVNP LFEKRPKNFG IGQDIQPKRD LTRFVKWPRY IRLQRQRAIL YKRLKVPPAI NQFTQALDR QTATQLLKLA HKYRPETKQE KKQRLLARAE KKAAGKGDVP TKRPPVLRAG VNTVTTLVEN KKAQLVVIAH D VDPIELVV FLPALCRKMG VPYCILKGKA RLGRLVHRKT CTTVAFTQVN SEDKGALAKL VEAIRTNYND RYDEIRRHWG GN VLGPKSV ARIAKLEKAK AKELATKLG

Macromolecule #49: 60S ribosomal protein L9

• Macromolecule: Name: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.871418 KDa

Sequence

String:

MKTILSNQTV DIPENVDISL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELVLEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #50: 60S ribosomal protein L10

• Macromolecule: Name: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.643057 KDa

Sequence

String:

MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS KKWGFTKFNA DEFEDMVAEK RLIPDGCGVK YIPNRGPLDK WRALHS

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #51: Ribosomal protein L11

• Macromolecule: Name: Ribosomal protein L11 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 20.288465 KDa

Sequence

String:

MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #52: Replicase polyprotein 1ab

• Macromolecule: Name: Replicase polyprotein 1ab / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Severe acute respiratory syndrome coronavirus 2
• Molecular weight: Theoretical: 121.565844 KDa
• Recombinant expression: Organism: Oryctolagus cuniculus (rabbit)

Sequence

String:

AGNATEVPAN STVLSFCAFA VDAAKAYKDY LASGGQPITN CVKMLCTHTG TGQAITVTPE ANMDQESFGG ASCCLYCRCH IDHPNPKGF CDLKGKYVQI PTTCANDPVG FTLKNTVCTV CGMWKGYGCS CDQLREPMLQ SADAQSFLNR VCGVSAARLT P CGTGTSTD VVYRAFDIYN DKVAGFAKFL KTNCCRFQEK DEDDNLIDSY FVVKRHTFSN YQHEETIYNL LKDCPAVAKH DF FKFRIDG DMVPHISRQR LTKYTMADLV YALRHFDEGN CDTLKEILVT YNCCDDDYFN KKDWYDFVEN PDILRVYANL GER VRQALL KTVQFCDAMR NAGIVGVLTL DNQDLNGNWY DFGDFIQTTP GSGVPVVDSY YSLLMPILTL TRALTAESHV DTDL TKPYI KWDLLKYDFT EERLKLFDRY FKYWDQTYHP NCVNCLDDRC ILHCANFNVL FSTVFPPTSF GPLVRKIFVD GVPFV VSTG YHFRELGVVH NQDVNLHSSR LSFKELLVYA ADPAMHAASG NLLLDKRTTC FSVAALTNNV AFQTVKPGNF NKDFYD FAV SKGFFKEGSS VELKHFFFAQ DGNAAISDYD YYRYNLPTMC DIRQLLFVVE VVDKYFDCYD GGCINANQVI VNNLDKS AG FPFNKWGKAR LYYDSMSYED QDALFAYTKR NVIPTITQMN LKYAISAKNR ARTVAGVSIC STMTNRQFHQ KLLKSIAA T RGATVVIGTS KFYGGWHNML KTVYSDVENP HLMGWDYPKC DRAMPNMLRI MASLVLARKH TTCCSLSHRF YRLANECAQ VLSEMVMCGG SLYVKPGGTS SGDATTAYAN SVFNICQAVT ANVNALLSTD GNKIADKYVR NLQHRLYECL YRNRDVDTDF VNEFYAYLR KHFSMMILSD DAVVCFNSTY ASQGLVASIK NFKSVLYYQN NVFMSEAKCW TETDLTKGPH EFCSQHTMLV K QGDDYVYL PYPDPSRILG AGCFVDDIVK TDGTLMIERF VSLAIDAYPL TKHPNQEYAD VFHLYLQYIR KLHDELTGHM LD MYSVMLT NDNTSRYWEP EFYEAMYTPH TVLQ

UniProtKB: Replicase polyprotein 1ab

Macromolecule #53: Ribosomal protein eL13

• Macromolecule: Name: Ribosomal protein eL13 / type: protein_or_peptide / ID: 53 / Details: Sequence from GeneBank entry XP_002710580.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.331723 KDa

Sequence

String:

MAPSRNGMIL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ ARARRIAPRP AAGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLVLFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V FKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

Macromolecule #54: Ribosomal protein L14

• Macromolecule: Name: Ribosomal protein L14 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 23.870549 KDa

Sequence

String:

MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG KKAAAQKAPA QKAPAQKAAG QKAAQPPKAQ KGQKPPAQKA PAPKASGKKA

UniProtKB: Large ribosomal subunit protein eL14

Macromolecule #55: Ribosomal protein L15

• Macromolecule: Name: Ribosomal protein L15 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.207285 KDa

Sequence

String:

MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

UniProtKB: Large ribosomal subunit protein eL15

Macromolecule #56: Ribosomal protein uL13

• Macromolecule: Name: Ribosomal protein uL13 / type: protein_or_peptide / ID: 56 / Details: Sequence from GeneBank entry XP_002723961.2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 23.533299 KDa

Sequence

String:

MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK ISKFTDVLKT HGLLV

Macromolecule #57: uL22

• Macromolecule: Name: uL22 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.444221 KDa

Sequence

String:

MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLKKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #58: Ribosomal Protein eL18

• Macromolecule: Name: Ribosomal Protein eL18 / type: protein_or_peptide / ID: 58 / Details: Sequence from GeneBank entry XP_008250645.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 21.699688 KDa

Sequence

String:

MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTVTDD VRVQEVPKLK VCALRVTSRA RSRILKAGGK ILTFDQLALD SPKGRGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

Macromolecule #59: 60S ribosomal protein L19

• Macromolecule: Name: 60S ribosomal protein L19 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 23.535281 KDa

Sequence

String:

MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

UniProtKB: Large ribosomal subunit protein eL19

Macromolecule #60: Ribosomal protein eL20

• Macromolecule: Name: Ribosomal protein eL20 / type: protein_or_peptide / ID: 60 / Details: Sequence from GeneBank entry GBCK01009614.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 20.749391 KDa

Sequence

String:

MKASGTLREY KVVGRCLPTP KSPAPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQVMKV EEMAAARCRR PAVKQFHDSR IRFPLPHRVL R RQHKPRFS AKRPNTFF

Macromolecule #61: eL21

• Macromolecule: Name: eL21 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 18.609988 KDa

Sequence

String:

MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

Macromolecule #62: Ribosomal protein eL22

• Macromolecule: Name: Ribosomal protein eL22 / type: protein_or_peptide / ID: 62 / Details: Sequence from GeneBank entry CU464830.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.756909 KDa

Sequence

String:

MAPVKKLVAK GGKKKKQVLK FTLDCTHPVE DGIMDAANFE QFLQERIKVN GKAGNLGGGA VTIERSKSKI TVTSEVPFSK RYLKYLTKK YLKKNNLRDW LRVVANSKES YELRYFQINQ DEEEEEDED

Macromolecule #63: Ribosomal protein L23

• Macromolecule: Name: Ribosomal protein L23 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.892505 KDa

Sequence

String:

MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #64: eL24

• Macromolecule: Name: eL24 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.825111 KDa

Sequence

String:

MKVELCSFSG YKIYPGHGRR YARTDGKVFQ FLNAKCESAF LSKRNPRQIN WTVLYRRKHK KGQSEEIQKK RTRRAVKFQR AITGASLAD IMAKRNQKPE VRKAQREQAI RAAKEAKKAK QASKKTAMAA AKAPTKAAPK QKIVKPVKVS APRVGGKR

UniProtKB: Large ribosomal subunit protein eL24

Macromolecule #65: uL23

• Macromolecule: Name: uL23 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.768246 KDa

Sequence

String:

MAPKAKKEAP APPKVEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #66: Ribosomal protein L26

• Macromolecule: Name: Ribosomal protein L26 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.303363 KDa

Sequence

String:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #67: 60S ribosomal protein L27

• Macromolecule: Name: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.835831 KDa

Sequence

String:

MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

UniProtKB: Large ribosomal subunit protein eL27

Macromolecule #68: 60S ribosomal protein L27a

• Macromolecule: Name: 60S ribosomal protein L27a / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 16.635551 KDa

Sequence

String:

MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGM(V5N)H HRINFDKYHP GYFGKVGMRH YHLKRNQSFC PTVNLD KLW TLVSEQTRVN AAKNKTGAAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKG VGGACVLVA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #69: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 26.721727 KDa

Sequence

String:

MAKS(MLZ)NHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALV KPK EVKPTIPKGV SRKLHRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGK AQ AETKPKAQAE TKPKAQAQAK PKAQAQGKPK AQAQGKPKAQ AQAKPKAQAQ AKPKAQAQTK PKAQATPAAP VPAQAPPK G AQPPAKAP

UniProtKB: Large ribosomal subunit protein eL29

Macromolecule #70: eL30

• Macromolecule: Name: eL30 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.807065 KDa

Sequence

String:

MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQSLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLSIIDP GDSDIIRSMP EQTGEK

UniProtKB: Large ribosomal subunit protein eL30

Macromolecule #71: eL31

• Macromolecule: Name: eL31 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.494938 KDa

Sequence

String:

MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

UniProtKB: Large ribosomal subunit protein eL31

Macromolecule #72: eL32

• Macromolecule: Name: eL32 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.898932 KDa

Sequence

String:

MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #73: eL33

• Macromolecule: Name: eL33 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.580809 KDa

Sequence

String:

MSGRLWCKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

Macromolecule #74: 60S ribosomal protein L34

• Macromolecule: Name: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 13.326074 KDa

Sequence

String:

MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT KKHVSRAYGG SMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQKAK

UniProtKB: Large ribosomal subunit protein eL34

Macromolecule #75: uL29

• Macromolecule: Name: uL29 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.566599 KDa

Sequence

String:

MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLNKHE ESLKTKKQQR KERLYPLRKY AVKA

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #76: 60S ribosomal protein L36

• Macromolecule: Name: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.263834 KDa

Sequence

String:

MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSSVLAAMRK AAAKKD

UniProtKB: Large ribosomal subunit protein eL36

Macromolecule #77: Ribosomal protein L37

• Macromolecule: Name: Ribosomal protein L37 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 11.111032 KDa

Sequence

String:

MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

UniProtKB: Large ribosomal subunit protein eL37

Macromolecule #78: eL38

• Macromolecule: Name: eL38 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 8.238948 KDa

Sequence

String:

MPRKIEEIKD FLLTARRKDA KSVKIKKNKD NVKFKVRCSR YLYTLVITDK EKAEKLKQSL PPGLAVKELK

UniProtKB: Large ribosomal subunit protein eL38

Macromolecule #79: eL39

• Macromolecule: Name: eL39 / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 6.455775 KDa

Sequence

String:

MSSHKTFRIK RFLAKKQKQN RPIPQWIWMK TGNKIRYNSK RRHWRRTKLG L

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #80: 60S ribosomal protein L40

• Macromolecule: Name: 60S ribosomal protein L40 / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 14.800474 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLRQLAQKY NCDKMICR(M3L)C YARLHPRAVN CRKKKCGHTN NLRPKKKVK

Macromolecule #81: eL42

• Macromolecule: Name: eL42 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 12.489991 KDa

Sequence

String:

MVNVPKTRRT FCKKCGKHQP HKVTQYKKGK DSLYAQGKRR YDRKQSGYGG QT(MLZ)PIFRKKA KTTKKIVLRL ECVEPN CRS KRMLAIKRCK HFELGGDKKR KGQVIQF

UniProtKB: Large ribosomal subunit protein eL42

Macromolecule #82: eL43

• Macromolecule: Name: eL43 / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 10.29935 KDa

Sequence

String:

MAKRTKKVGI VGKYGTRYGA SLRKMVKKIE ISQHAKYTCS FCGKTKMKRR AVGIWHCGSC MKTVAGGAWT YNTTSAVTVK SAIRRLKEL KDQ

UniProtKB: Large ribosomal subunit protein eL43

Macromolecule #83: Ribosomal protein eL28

• Macromolecule: Name: Ribosomal protein eL28 / type: protein_or_peptide / ID: 83 / Details: Sequence from GeneBank entry GBCM01003096.1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 15.844696 KDa

Sequence

String:

M(SAC)AHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVMKRRSGQ RKPATS YVR TTINKNARAT LSSIRHMIRK NKYRPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

Macromolecule #84: 60S acidic ribosomal protein P0

• Macromolecule: Name: 60S acidic ribosomal protein P0 / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 34.380504 KDa

Sequence

String:

MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNVGSKQM QQIRMSLRGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ L IKTGDKVG ASEATLLNML NISPFSFGLI IQQVFDNGSI YNPEVLDITE DTLHSRFLEG VRNVASVCLQ IGYPTVASVP HS IINGYKR VLALSVETEY TFPLAEKVKA FLADPSAFVA AAPVAAASTA APAAAAAAPA KVEAKEESEE SDEDMGFGLF D

UniProtKB: Large ribosomal subunit protein uL10

Macromolecule #85: Ribosomal protein L12

• Macromolecule: Name: Ribosomal protein L12 / type: protein_or_peptide / ID: 85 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 17.847619 KDa

Sequence

String:

MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECPAS

UniProtKB: Large ribosomal subunit protein uL11

Macromolecule #86: Ribosomal protein uL1

• Macromolecule: Name: Ribosomal protein uL1 / type: protein_or_peptide / ID: 86 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Oryctolagus cuniculus (rabbit)
• Molecular weight: Theoretical: 24.87541 KDa

Sequence

String:

MGDILSRDTL YEAVREVLHG NQRKRRKFLE TVELQISLKN YDPQKDKRFS GTVRLKSTPR PKFSVCVLGD QQHCDEAKAV DIPHMDIEA LKKLNKNKKL VKKLAKKYDA FLASESLIKQ IPRILGPGLN KAGKFPSLLT HNENMVAKVD EVKSTIKFQM K KVLCLAVA VGHVKMTDDE LVYNIHLAVN FLVSLLKKNW QNVRALYIKS TMGKPQRLY

UniProtKB: Large ribosomal subunit protein uL1

Macromolecule #87: SPERMIDINE

• Macromolecule: Name: SPERMIDINE / type: ligand / ID: 87 / Number of copies: 30 / Formula: SPD
• Molecular weight: Theoretical: 145.246 Da

Chemical component information

ChemComp-SPD:
SPERMIDINE

Macromolecule #88: SPERMINE

• Macromolecule: Name: SPERMINE / type: ligand / ID: 88 / Number of copies: 3 / Formula: SPM
• Molecular weight: Theoretical: 202.34 Da

Chemical component information

ChemComp-SPM:
SPERMINE

Macromolecule #89: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 89 / Number of copies: 420 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #90: UNKNOWN ATOM OR ION

• Macromolecule: Name: UNKNOWN ATOM OR ION / type: ligand / ID: 90 / Number of copies: 329 / Formula: UNX

Macromolecule #91: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 91 / Number of copies: 8 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #92: water

• Macromolecule: Name: water / type: ligand / ID: 92 / Number of copies: 2117 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.3 mg/mL
• Buffer: pH: 7.4
• Grid: Model: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 1 / Pretreatment - Type: GLOW DISCHARGE
• Vitrification: Cryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Specialist optics: Energy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 100.0 µm / Calibrated magnification: 56604 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal magnification: 81000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Startup model: Type of model: OTHER
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 2) / Number images used: 171706
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 2)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 2)