- EMDB-10647: CryoEM structure of the wide type IV pilus (PilA4) from Thermus t... -
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基本情報
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データベース: EMDB / ID: EMD-10647
タイトル
CryoEM structure of the wide type IV pilus (PilA4) from Thermus thermophilus
マップデータ
試料
複合体: Thermus thermophilus wide pilus
タンパク質・ペプチド: PilA
機能・相同性
機能・相同性情報
protein secretion by the type II secretion system / type II protein secretion system complex / cell outer membrane / membrane => GO:0016020 / periplasmic space / plasma membrane 類似検索 - 分子機能
Bacterial general secretion pathway protein G-type pilin / Prokaryotic N-terminal methylation site. / Prokaryotic N-terminal methylation motif / Prokaryotic N-terminal methylation site 類似検索 - ドメイン・相同性
Type IV wide pilus major component PilA4 類似検索 - 構成要素
Biotechnology and Biological Sciences Research Council (BBSRC)
BB/R008639/1
英国
引用
ジャーナル: Nat Commun / 年: 2020 タイトル: Cryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium. 著者: Alexander Neuhaus / Muniyandi Selvaraj / Ralf Salzer / Julian D Langer / Kerstin Kruse / Lennart Kirchner / Kelly Sanders / Bertram Daum / Beate Averhoff / Vicki A M Gold / 要旨: Type IV pili are flexible filaments on the surface of bacteria, consisting of a helical assembly of pilin proteins. They are involved in bacterial motility (twitching), surface adhesion, biofilm ...Type IV pili are flexible filaments on the surface of bacteria, consisting of a helical assembly of pilin proteins. They are involved in bacterial motility (twitching), surface adhesion, biofilm formation and DNA uptake (natural transformation). Here, we use cryo-electron microscopy and mass spectrometry to show that the bacterium Thermus thermophilus produces two forms of type IV pilus ('wide' and 'narrow'), differing in structure and protein composition. Wide pili are composed of the major pilin PilA4, while narrow pili are composed of a so-far uncharacterized pilin which we name PilA5. Functional experiments indicate that PilA4 is required for natural transformation, while PilA5 is important for twitching motility.
名称: PilA / タイプ: protein_or_peptide / ID: 1 詳細: Sequence corresponds to the mature protein. The first 6 residues are removed by prepilin peptidase. コピー数: 16 / 光学異性体: LEVO