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- EMDB-10446: SAGA-TBP filtered to show the position of the DUB domain -

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基本情報

登録情報
データベース: EMDB / ID: EMD-10446
タイトルSAGA-TBP filtered to show the position of the DUB domain
マップデータ
試料
  • 複合体: SAGA bound to TBP
    • 複合体: TATA-box binding protein (TBP)
      • タンパク質・ペプチド: x 1種
    • 複合体: SAGA component
      • タンパク質・ペプチド: x 1種
    • 複合体: Polyubiquitin-B
      • タンパク質・ペプチド: x 1種
    • 複合体: SAGA complex
      • タンパク質・ペプチド: x 15種
  • リガンド: x 1種
キーワードTranscriptional co-activator / Histone-acetylation / TRANSCRIPTION
機能・相同性
機能・相同性情報


SAGA-type complex / RITS complex assembly / regulation of primary metabolic process / DUBm complex / positive regulation of DNA-templated transcription initiation / TFIIA-class transcription factor complex binding / RNA polymerase III transcription regulatory region sequence-specific DNA binding / regulatory ncRNA-mediated heterochromatin formation / RNA polymerase III preinitiation complex assembly / transcription export complex 2 ...SAGA-type complex / RITS complex assembly / regulation of primary metabolic process / DUBm complex / positive regulation of DNA-templated transcription initiation / TFIIA-class transcription factor complex binding / RNA polymerase III transcription regulatory region sequence-specific DNA binding / regulatory ncRNA-mediated heterochromatin formation / RNA polymerase III preinitiation complex assembly / transcription export complex 2 / transcription factor TFIIIB complex / nuclear mRNA surveillance / RNA polymerase I general transcription initiation factor binding / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / SLIK (SAGA-like) complex / regulation of transcription by RNA polymerase III / transcription factor TFIIA complex / RNA polymerase I preinitiation complex assembly / hypothalamus gonadotrophin-releasing hormone neuron development / SAGA complex / female meiosis I / DNA binding, bending / positive regulation of protein monoubiquitination / fat pad development / mitochondrion transport along microtubule / RNA Polymerase III Transcription Initiation From Type 2 Promoter / poly(A)+ mRNA export from nucleus / RNA polymerase II transcribes snRNA genes / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / RNA Polymerase II Pre-transcription Events / female gonad development / seminiferous tubule development / NuA4 histone acetyltransferase complex / RNA Polymerase I Promoter Escape / male meiosis I / nucleolar large rRNA transcription by RNA polymerase I / Estrogen-dependent gene expression / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / protein deubiquitination / nuclear pore / RNA polymerase II core promoter sequence-specific DNA binding / RNA polymerase II preinitiation complex assembly / energy homeostasis / regulation of neuron apoptotic process / regulation of proteasomal protein catabolic process / Maturation of protein E / Maturation of protein E / ER Quality Control Compartment (ERQC) / Myoclonic epilepsy of Lafora / FLT3 signaling by CBL mutants / Prevention of phagosomal-lysosomal fusion / IRAK2 mediated activation of TAK1 complex / Alpha-protein kinase 1 signaling pathway / Glycogen synthesis / IRAK1 recruits IKK complex / IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation / Membrane binding and targetting of GAG proteins / Endosomal Sorting Complex Required For Transport (ESCRT) / Regulation of TBK1, IKKε (IKBKE)-mediated activation of IRF3, IRF7 / Negative regulation of FLT3 / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / Regulation of TBK1, IKKε-mediated activation of IRF3, IRF7 upon TLR3 ligation / Constitutive Signaling by NOTCH1 HD Domain Mutants / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / NOTCH2 Activation and Transmission of Signal to the Nucleus / TICAM1,TRAF6-dependent induction of TAK1 complex / TICAM1-dependent activation of IRF3/IRF7 / APC/C:Cdc20 mediated degradation of Cyclin B / Regulation of FZD by ubiquitination / Downregulation of ERBB4 signaling / APC-Cdc20 mediated degradation of Nek2A / p75NTR recruits signalling complexes / InlA-mediated entry of Listeria monocytogenes into host cells / TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling / TRAF6-mediated induction of TAK1 complex within TLR4 complex / Regulation of pyruvate metabolism / Regulation of innate immune responses to cytosolic DNA / NF-kB is activated and signals survival / Downregulation of ERBB2:ERBB3 signaling / Pexophagy / NRIF signals cell death from the nucleus / VLDLR internalisation and degradation / Regulation of PTEN localization / TBP-class protein binding / Activated NOTCH1 Transmits Signal to the Nucleus / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Regulation of BACH1 activity / MAP3K8 (TPL2)-dependent MAPK1/3 activation / TICAM1, RIP1-mediated IKK complex recruitment / Translesion synthesis by REV1 / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) / Translesion synthesis by POLK / InlB-mediated entry of Listeria monocytogenes into host cell / Downregulation of TGF-beta receptor signaling / Josephin domain DUBs
類似検索 - 分子機能
Histone acetyltransferases subunit 3 / Histone acetyltransferases subunit 3 / Transcriptional activator Spt7 / Transcriptional coactivator Hfi1/Transcriptional adapter 1 / Transcriptional regulator of RNA polII, SAGA, subunit / Transcription factor Spt20 / Spt20-like, SEP domain / Spt20, SEP domain / SAGA complex, Sgf11 subunit / Sgf11 (transcriptional regulation protein) ...Histone acetyltransferases subunit 3 / Histone acetyltransferases subunit 3 / Transcriptional activator Spt7 / Transcriptional coactivator Hfi1/Transcriptional adapter 1 / Transcriptional regulator of RNA polII, SAGA, subunit / Transcription factor Spt20 / Spt20-like, SEP domain / Spt20, SEP domain / SAGA complex, Sgf11 subunit / Sgf11 (transcriptional regulation protein) / Transcription factor, enhancer of yellow 2 / Transcription factor EnY2 superfamily / Transcription factor e(y)2 / SAGA-associated factor 73 / LisH / SCA7 domain / SCA7, zinc-binding domain / SCA7 domain profile. / Tra1, HEAT repeat ring region / Tra1, HEAT repeat central region / Tra1 HEAT repeat central region / Tra1 HEAT repeat ring region / Transcription initiation factor IID, subunit 13 / Transcription initiation factor IID, 18kD subunit / : / Transcription initiation factor IID, 31kD subunit / Transcription initiation factor TFIID subunit 12 / Transcription initiation factor TFIID subunit A / TFIID subunit TAF5, NTD2 domain superfamily / WD40 associated region in TFIID subunit, NTD2 domain / Transcription initiation factor TAFII31 / TATA box binding protein associated factor (TAF) / Transcription initiation factor TFIID subunit 12 domain / TFIID subunit TAF5, NTD2 domain / Transcription initiation factor TFIID subunit 6 / TAF6, C-terminal HEAT repeat domain superfamily / TAF6 C-terminal HEAT repeat domain / TAF6, C-terminal HEAT repeat domain / Transcription initiation factor TFIID 23-30kDa subunit / Transcription initiation factor TFIID, 23-30kDa subunit / Lissencephaly type-1-like homology motif / : / Ubiquitin Carboxyl-terminal Hydrolase-like zinc finger / Zinc finger, UBP-type / Zn-finger in ubiquitin-hydrolases and other protein / Zinc finger UBP-type profile. / LIS1 homology (LisH) motif profile. / LIS1 homology motif / Ubiquitin specific protease (USP) domain signature 2. / Ubiquitin specific protease (USP) domain signature 1. / Ubiquitin specific protease, conserved site / TATA-box binding protein, eukaryotic / : / TATA-box binding protein / TATA-box binding protein, conserved site / Transcription factor TFIID (or TATA-binding protein, TBP) / Transcription factor TFIID repeat signature. / PIK-related kinase, FAT / FAT domain / FATC / Peptidase C19, ubiquitin carboxyl-terminal hydrolase / Ubiquitin carboxyl-terminal hydrolase / FATC domain / PIK-related kinase / FAT domain profile. / FATC domain profile. / Ubiquitin specific protease domain / Ubiquitin specific protease (USP) domain profile. / TBP domain superfamily / Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily / Phosphoinositide 3-kinase, catalytic domain / Phosphatidylinositol 3- and 4-kinase / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / Papain-like cysteine peptidase superfamily / TATA box binding protein associated factor / TATA box binding protein associated factor (TAF), histone-like fold domain / : / Ubiquitin domain signature. / Ubiquitin conserved site / Ubiquitin domain / Bromodomain, conserved site / Bromodomain signature. / Histone-fold / Bromodomain / bromo domain / Bromodomain (BrD) profile. / Bromodomain / Bromodomain-like superfamily / Ubiquitin family / Ubiquitin homologues / Ubiquitin domain profile. / Ubiquitin-like domain / WD domain, G-beta repeat / Armadillo-type fold / Zinc finger, RING/FYVE/PHD-type / Ubiquitin-like domain superfamily / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature.
類似検索 - ドメイン・相同性
Subunit (60 kDa) of TFIID and SAGA complexes / Transcription initiation factor TFIID subunit 10 / Ubiquitin carboxyl-terminal hydrolase / Transcription-associated protein / Spt20-like SEP domain-containing protein / Subunit of SAGA histone acetyltransferase complex / Transcriptional coactivator HFI1/ADA1 / Transcription initiation factor TFIID subunit 9 / Transcription initiation factor TFIID subunit 12 / Transcription and mRNA export factor SUS1 ...Subunit (60 kDa) of TFIID and SAGA complexes / Transcription initiation factor TFIID subunit 10 / Ubiquitin carboxyl-terminal hydrolase / Transcription-associated protein / Spt20-like SEP domain-containing protein / Subunit of SAGA histone acetyltransferase complex / Transcriptional coactivator HFI1/ADA1 / Transcription initiation factor TFIID subunit 9 / Transcription initiation factor TFIID subunit 12 / Transcription and mRNA export factor SUS1 / Transcriptional regulator involved in glucose repression of Gal4p-regulated genes / SAGA-associated factor 11 / Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to act / Transcription initiation factor TFIID subunit 5 / Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex / Ubiquitin B / Polyubiquitin-B / TATA-box-binding protein
類似検索 - 構成要素
生物種Komagataella phaffii GS115 (菌類) / Homo sapiens (ヒト) / Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
手法単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 20.0 Å
データ登録者Papai G / Frechard A
資金援助 フランス, 3件
OrganizationGrant number国
French National Research AgencyANR-15-CE11-0022-01 フランス
French National Research AgencyANR-10-LABX-0030-INRT フランス
French National Research AgencyANR-10-IDEX-0002-02 フランス
引用ジャーナル: Nature / 年: 2020
タイトル: Structure of SAGA and mechanism of TBP deposition on gene promoters.
著者: Gabor Papai / Alexandre Frechard / Olga Kolesnikova / Corinne Crucifix / Patrick Schultz / Adam Ben-Shem /
要旨: SAGA (Spt-Ada-Gcn5-acetyltransferase) is a 19-subunit complex that stimulates transcription via two chromatin-modifying enzymatic modules and by delivering the TATA box binding protein (TBP) to ...SAGA (Spt-Ada-Gcn5-acetyltransferase) is a 19-subunit complex that stimulates transcription via two chromatin-modifying enzymatic modules and by delivering the TATA box binding protein (TBP) to nucleate the pre-initiation complex on DNA, a pivotal event in the expression of protein-encoding genes. Here we present the structure of yeast SAGA with bound TBP. The core of the complex is resolved at 3.5 Å resolution (0.143 Fourier shell correlation). The structure reveals the intricate network of interactions that coordinate the different functional domains of SAGA and resolves an octamer of histone-fold domains at the core of SAGA. This deformed octamer deviates considerably from the symmetrical analogue in the nucleosome and is precisely tuned to establish a peripheral site for TBP, where steric hindrance represses binding of spurious DNA. Complementary biochemical analysis points to a mechanism for TBP delivery and release from SAGA that requires transcription factor IIA and whose efficiency correlates with the affinity of DNA to TBP. We provide the foundations for understanding the specific delivery of TBP to gene promoters and the multiple roles of SAGA in regulating gene expression.
履歴
登録2019年11月1日-
ヘッダ(付随情報) 公開2020年2月12日-
マップ公開2020年2月12日-
更新2024年9月25日-
現状2024年9月25日処理サイト: PDBe / 状態: 公開

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構造の表示

ムービー
  • 表面図（断面を密度値に従い着色）
  • 表面レベル: 0.004
  • UCSF Chimeraによる作画
  • ダウンロード
  • 表面図（半径に従い着色）
  • 表面レベル: 0.004
  • UCSF Chimeraによる作画
  • ダウンロード
  • あてはめたモデルとの重ね合わせ
  • 原子モデル: PDB-6tbm
  • 表面レベル: 0.004
  • UCSF Chimeraによる作画
  • ダウンロード
ムービービューア
構造ビューアEMマップ:
SurfViewMolmilJmol/JSmol
添付画像

emd_10446.png

ダウンロードとリンク

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マップ

ファイルダウンロード / ファイル: emd_10446.map.gz / 形式: CCP4 / 大きさ: 64 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
投影像・断面図

画像のコントロール

大きさ
明度
コントラスト
その他
軸Z (Sec.)Y (Row.)X (Col.)
2.18 Å/pix.
x 256 pix.
= 558.08 Å		2.18 Å/pix.
x 256 pix.
= 558.08 Å		2.18 Å/pix.
x 256 pix.
= 558.08 Å

表面

投影像

断面 (1/3)

断面 (1/2)

断面 (2/3)

画像は Spider により作成

ボクセルのサイズX=Y=Z: 2.18 Å
密度

ヒストグラム

ヒストグラム (対数)
表面レベル登録者による: 0.004 / ムービー #1: 0.004
最小 - 最大-0.023929587 - 0.031576734
平均 (標準偏差)0.000009551157 (±0.0011494906)
対称性空間群: 1
詳細

EMDB XML:

マップ形状
Axis orderXYZ
Origin000
サイズ256256256
Spacing256256256
セルA=B=C: 558.08 Å
α=β=γ: 90.0 °

CCP4マップ ヘッダ情報:

modeImage stored as Reals
Å/pix. X/Y/Z2.182.182.18
M x/y/z256256256
origin x/y/z0.0000.0000.000
length x/y/z558.080558.080558.080
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS256256256
D min/max/mean-0.0240.0320.000

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添付データ

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試料の構成要素

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全体 : SAGA bound to TBP

全体名称: SAGA bound to TBP
要素
  • 複合体: SAGA bound to TBP
    • 複合体: TATA-box binding protein (TBP)
      • タンパク質・ペプチド: TATA-box Binding Protein (TBP)
    • 複合体: SAGA component
      • タンパク質・ペプチド: Subunit of SAGA histone acetyltransferase complex
    • 複合体: Polyubiquitin-B
      • タンパク質・ペプチド: Polyubiquitin-B
    • 複合体: SAGA complex
      • タンパク質・ペプチド: Transcriptional coactivator HFI1/ADA1
      • タンパク質・ペプチド: Spt20
      • タンパク質・ペプチド: Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to act
      • タンパク質・ペプチド: Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex
      • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 10
      • タンパク質・ペプチド: Subunit (61/68 kDa) of TFIID and SAGA complexes
      • タンパク質・ペプチド: Subunit (90 kDa) of TFIID and SAGA complexes
      • タンパク質・ペプチド: Subunit (60 kDa) of TFIID and SAGA complexes
      • タンパク質・ペプチド: Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation
      • タンパク質・ペプチド: Transcription-associated protein
      • タンパク質・ペプチド: Transcriptional regulator involved in glucose repression of Gal4p-regulated genes
      • タンパク質・ペプチド: Spt8
      • タンパク質・ペプチド: Ubiquitin carboxyl-terminal hydrolase
      • タンパク質・ペプチド: SAGA-associated factor 11
      • タンパク質・ペプチド: Transcription and mRNA export factor SUS1
  • リガンド: water

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超分子 #1: SAGA bound to TBP

超分子名称: SAGA bound to TBP / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#18
分子量理論値: 1.6 MDa

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超分子 #2: TATA-box binding protein (TBP)

超分子名称: TATA-box binding protein (TBP) / タイプ: complex / ID: 2 / 親要素: 1 / 含まれる分子: #1
由来(天然)生物種: Komagataella phaffii GS115 (菌類)

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超分子 #3: SAGA component

超分子名称: SAGA component / タイプ: complex / ID: 3 / 親要素: 1 / 含まれる分子: #3
由来(天然)生物種: Komagataella phaffii GS115 (菌類)

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超分子 #4: Polyubiquitin-B

超分子名称: Polyubiquitin-B / タイプ: complex / ID: 4 / 親要素: 1 / 含まれる分子: #15
由来(天然)生物種: Homo sapiens (ヒト)

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超分子 #5: SAGA complex

超分子名称: SAGA complex / タイプ: complex / ID: 5 / 親要素: 1 / 含まれる分子: #2, #4-#14, #16-#18
由来(天然)生物種: Komagataella phaffii GS115 (菌類)

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分子 #1: TATA-box Binding Protein (TBP)

分子名称: TATA-box Binding Protein (TBP) / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
分子量理論値: 27.042275 KDa
組換発現生物種: Escherichia coli (大腸菌)
配列文字列: MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHA RNAEYNPKRF AAVIMRIREP KTTALIFASG KMVVTGAKSE DDSKLASRKY ARIIQKIGFA AKFTDFKIQN I VGSCDVKF ...文字列:
MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT SGIVPTLQNI VATVTLGCRL DLKTVALHA RNAEYNPKRF AAVIMRIREP KTTALIFASG KMVVTGAKSE DDSKLASRKY ARIIQKIGFA AKFTDFKIQN I VGSCDVKF PIRLEGLAFS HGTFSSYEPE LFPGLIYRMV KPKIVLLIFV SGKIVLTGAK QREEIYQAFE AIYPVLSEFR KM

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分子 #2: Transcriptional coactivator HFI1/ADA1

分子名称: Transcriptional coactivator HFI1/ADA1 / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 49.888273 KDa
配列文字列: MASTELVAST PMPSRNGQGS HQKLNGGNVN SATNTPTVAS TPVNQPRKLP SENFSGLGGA IRIEIEPLVV DFQRRLGKNW GRYQIAVSL FLVGKLSRRE LVDELDDILD RNTVKMHNQL LLGNLANSLK ESAGDRVGSG GFGGSMFNKR VKDSRKSSQY E RLKRDILA ...文字列:
MASTELVAST PMPSRNGQGS HQKLNGGNVN SATNTPTVAS TPVNQPRKLP SENFSGLGGA IRIEIEPLVV DFQRRLGKNW GRYQIAVSL FLVGKLSRRE LVDELDDILD RNTVKMHNQL LLGNLANSLK ESAGDRVGSG GFGGSMFNKR VKDSRKSSQY E RLKRDILA LPVRERRRIK AITRESGKKG MVNSVITQTR QALIPKVPVV TRPTNVPGNS VQWAQDVIHG FQALLASEIY EL PEMDNLR TRMTGISREH GLIGPVDDSV IEIMLIGLEQ HLKGIVEAAI DIVKYRRTKY TNNSVVDTLQ KEQTNTSDTS SSK VSRKRD RHTLTIEDMY DTLEQYPYLV EPGGTLLRLQ SVMLHDEDEL SDDEILKHIL KPRTSDSTDR KPKQLQENGT ADSL SKVTK SITNGANASG DAVPNGKAEH IQNTMGTKEE LNWFIYDILS NNE

UniProtKB: Transcriptional coactivator HFI1/ADA1

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分子 #3: Subunit of SAGA histone acetyltransferase complex

分子名称: Subunit of SAGA histone acetyltransferase complex / タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 79.995227 KDa
組換発現生物種: Escherichia coli (大腸菌)
配列文字列: MQNPERPVVW KELGQYLDTL QPIHQEKPSS LPDLPSPSYK DSLSNPLRYR ICNNCDRPIL QSCLSKHIKI CNQIVKVEKL EEDDKPMNM VRKRKNQVLE ESKESTPVTN TNSTNSSNAI NSTTSKKTKK VKLPKEKKPK KERVKSTAKP KGPVDVERQC G VPLPNGGY ...文字列:
MQNPERPVVW KELGQYLDTL QPIHQEKPSS LPDLPSPSYK DSLSNPLRYR ICNNCDRPIL QSCLSKHIKI CNQIVKVEKL EEDDKPMNM VRKRKNQVLE ESKESTPVTN TNSTNSSNAI NSTTSKKTKK VKLPKEKKPK KERVKSTAKP KGPVDVERQC G VPLPNGGY CARSLTCKTH SMGAKRAVPG RSAPYDQLLV AYQRKNQAKF AANAAAAHAA RDDLIHGSQI PIDEDDEAHQ VL DGVAKSY PLPLERKVIM PARSRSSFLR MREMFAGAIL PRVPTNPFGN LYSRTAVIDV DRAGDYYFSV RASPRMPNQQ QNK TQKNPQ PPRPQVQQQQ QAQKSQQSPP QAQQQISQSA GQQTSQTVQR LVQQQQRLQQ QRQQTQIQQQ QQHQQQQQQQ QHQQ QQQQQ QQQQQQQTHM LQQQQQQQQQ QQMLLLQQQQ QQQAQQQQSP PITHQSPIPD NRPSNQLPQQ YSQMTPQQLQ LQQQL QLQQ KQKEQFRLQQ QQHLRNL(UNK)MR (UNK)TPQQQQQFA NLTPEKRAQF QRMQLVQLQQ QIKNQAIQNQ MQQQQQQP S HVQSQAQTQF QQAQLKAQSR AQVLQAAQRQ MQNSPTSPVT SMSSPLAIPS NLANQGPNQT SQLPNEQLSN PLMGNQFQG QFNQYQPNYR ENLEGGGGSM DEKTTGWRGG HVVEGLAGEL EQLRARLEHH PQGQREPGGS HHHHHH

UniProtKB: Subunit of SAGA histone acetyltransferase complex

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分子 #4: Spt20

分子名称: Spt20 / タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 59.530578 KDa
配列文字列: MSQIQSSQVG AKNMQTAQPQ AQQRPINGSV TLSNGQRINP QNLTPQQQRL LQQKVLHQKL KNYKFAETSE EILHKYEKFP PSLTFHIHE NHYRFGDQDG VIPKNSSVIK AFLEYVAREE IPPALIEVTK DAGVQLYEGC IILRIYDHRH LVAHINDAKD S QHVSSESS ...文字列:
MSQIQSSQVG AKNMQTAQPQ AQQRPINGSV TLSNGQRINP QNLTPQQQRL LQQKVLHQKL KNYKFAETSE EILHKYEKFP PSLTFHIHE NHYRFGDQDG VIPKNSSVIK AFLEYVAREE IPPALIEVTK DAGVQLYEGC IILRIYDHRH LVAHINDAKD S QHVSSESS ANNTNNEKKE PSKVPKTYTT ILRPTQLSLY ADLLYQTDYL QVRFTDSLSL NIETEILTAS KRNLDLSVPL NP HRATANL KPEVKYPYYD PETDTVVHEH RPDARGNMVN ADTVDYRKLH EDMAQHGSDY ERLMLLLSDR FDDDSQTQGS SVT SSQFMR LRFVEAWRKK EERLQESGGS SQRQRPLQPG QIPGMLSSFS SNTGNMNTML DNLTPQQKAA IQQRMIQQGR AQPQ QQQPD QEQLQEQQQQ QPQQAMSQQR QQQLQQLYQR QQQHNQHHQQ NQDELSSLDD KKIKKPPAKK QKKMTKKEMT TLHQN VAGK TIPSAESTPP TTAANKKRGT YKKKNKYILI IND

UniProtKB: Spt20-like SEP domain-containing protein

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分子 #5: Subunit of the SAGA and SAGA-like transcriptional regulatory comp...

分子名称: Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to act
タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 39.030105 KDa
配列文字列: MTDAKDKDHK YRYRMEIQQM MFVSGETNDP PVETTSLIED IVRSQVVEIV LHSSQTALSR GTKSIVPEDV IFLIRHDKAK VNRLRTYLS WKDVRKNAKD QQSGDLADIG GDDLLDNSTS QVAASGAPGA PGSSSSTSMD SKMLSKYKKS KIRLPWELQF V FTEQPLDT ...文字列:
MTDAKDKDHK YRYRMEIQQM MFVSGETNDP PVETTSLIED IVRSQVVEIV LHSSQTALSR GTKSIVPEDV IFLIRHDKAK VNRLRTYLS WKDVRKNAKD QQSGDLADIG GDDLLDNSTS QVAASGAPGA PGSSSSTSMD SKMLSKYKKS KIRLPWELQF V FTEQPLDT NEDDAEDEDE RAATLASLKR LKTNDERTKN MTKEEYVHWS ECRQASFTFR KAKRFREWCG LSHLAESRPS DD VIDILGF LTFEMVCSIT EEALIVKMLE EQNGDLASST TTIQKLQESH RKRKHLFDGP DKDVRPITSG HVLEAWRRLQ KRN VEKKAI RNFQGGKLRS RVQLI

UniProtKB: Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to act

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分子 #6: Subunit of the SAGA transcriptional regulatory complex, involved ...

分子名称: Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex
タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 136.768828 KDa
配列文字列: MANERALQYF VTSDNQRLYD LAAKLFSLGF FNCYFTPQQF QLLSVILDNG KLRIEEDGFV STVWDEFIKG RLVLQFSKGD YEKESTKHT VDNVKGTDDK TGIEVSKENG HSSDPPSADH DITESDNVGR EETLDDQFSE LYLKDGVSTS QFMCAKLRYL L FEQAIDYL ...文字列:
MANERALQYF VTSDNQRLYD LAAKLFSLGF FNCYFTPQQF QLLSVILDNG KLRIEEDGFV STVWDEFIKG RLVLQFSKGD YEKESTKHT VDNVKGTDDK TGIEVSKENG HSSDPPSADH DITESDNVGR EETLDDQFSE LYLKDGVSTS QFMCAKLRYL L FEQAIDYL YTNSSMNGDA EEYSLLQNVD DMNEEKEETA KPATPQNVRE IDNDYDDDED EDEEDEEIKE ASSKNPFKTE DN EPLTNER QPTNNVFIRL CSDEKSLSKS LTLNLLTSMV TSKPEYPVEQ LKGSEVIGTS HPLLGTASAI ESKLEKRNEA RLI KNFSKI YHQFDKDERN FLKRRKLEIS NKQFLDQDNN DPKDRPSSNS TSDEESNSNN LEKTSQPSSV NKLMQLGGAA NLSL KNLLS KVEENREKLN LSDLDLRSLI MDVRKNRSKW ASDDKIGQEE LYEACEKVVL ELRGYTEHST AFLNRVSKRE APNYY QIIK KPMDLNTVMK KLKSFQYKSK QEFVDDLMLI WKNCLTYNSD PSHFIRVHAV AMQKKTLSLI PLIPDIVIRD RSELEK DEV ENVVETPQSS SIGTPMTTRV AGGKGPKKGR THREPPTRPE TPDGIAGEDS SQVSKAEQDS PAKALEVTEN DSTKEKE KD LSDVPTQEQK NDKTEKDDDY QEAVEDEEED DDDNTNLEED STEDEDDLEV QTWNSLTSNV RYKICEKRRN LFKENKIQ P NEEAIFRDTY QMENFMHYLG DDVRIVLNTP MSRYQYYDGN EDPYLIEYDI SGGIPGLKYS GVSPDEGDLE DNMLVDQLM KGESSIRESG FARKPTGMNK KFNEIIHLMQ QIRRICFKIS LIRQMQTQQF LHHTQMKPPD INEIQDIDID QLSNLPNRDK LDSDVSYNS LKRSISKILM ANGFESTAPF CSDVITQIAE NYFGNLVKTL KNHIESKSIN KVMGDAKFQK VSNKDILLLS L LENGVESP DALYEYYNEN IVKQISKLEG LKSRLSSFLA ELLRPGLQDL NERQFNDNSD EFLTGNFSSE IGDDFFGFRE LG LEKEFGL LTSTVPLHLL HSRLNSSIFN SNHEVSQLKF DDIQAEEKEQ LYKREIPQHI KLIQPFLYNI AEKSKTIHYK QLK KLNELQ KMPENDDDIL LIEDEDLPLK QRNTRPRVPP TGKIPNVKKK AVNGAYFLDP AIFSSNNQVK VEGDT

UniProtKB: Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex

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分子 #7: Transcription initiation factor TFIID subunit 10

分子名称: Transcription initiation factor TFIID subunit 10 / タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 23.879145 KDa
配列文字列: MSTEKLFEDL DEDSLMQDVE GGVEGSMTIE EEPEPNDEKE APHISMPSLP EYTRKDKTLE EILEMMEDEE FTPIIPDAVT DYYLAKNGF ETSDIKIKRI LALATQKFIS DIAQDAYEYS RIRSSSSVYT SANPQARARQ LVAGQQQQQQ QQPQQQQQQQ P QTGASQPA ...文字列:
MSTEKLFEDL DEDSLMQDVE GGVEGSMTIE EEPEPNDEKE APHISMPSLP EYTRKDKTLE EILEMMEDEE FTPIIPDAVT DYYLAKNGF ETSDIKIKRI LALATQKFIS DIAQDAYEYS RIRSSSSVYT SANPQARARQ LVAGQQQQQQ QQPQQQQQQQ P QTGASQPA PTVGGSGGGG GGGGGTSTGT NNKVVLTMDD LRSALGEYGI NVKRPNFYR

UniProtKB: Transcription initiation factor TFIID subunit 10

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分子 #8: Subunit (61/68 kDa) of TFIID and SAGA complexes

分子名称: Subunit (61/68 kDa) of TFIID and SAGA complexes / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 66.690133 KDa
配列文字列: MNQPPNQPQD NDSQVSSSGM QGGNSMNYSS SAPVNGAGQG PQPQQNSQGQ NGKKPVGMQQ AQIQLLARRY QLEMQKAHQL GPQTPQGAE HLQTATKIKH VLLSYQQQRQ RQQGQVQGQG LSQPQGQNQA QGRVQQQTQG LSPDPGSHQG SPQFQQPHQV M MGSAQTAG ...文字列:
MNQPPNQPQD NDSQVSSSGM QGGNSMNYSS SAPVNGAGQG PQPQQNSQGQ NGKKPVGMQQ AQIQLLARRY QLEMQKAHQL GPQTPQGAE HLQTATKIKH VLLSYQQQRQ RQQGQVQGQG LSQPQGQNQA QGRVQQQTQG LSPDPGSHQG SPQFQQPHQV M MGSAQTAG TSIANPQAQS NISSQVSQMA AAKVNSASPP IPPKTVGTPT GTPVGTQPRG QVTIQQFQQV KSILEEFEKK LR TIEAAKR DQNLPEETLQ KLLRQEAILK QRYAQTKATA YQMSQHLQRQ QQALRAASAE SAEVYVTGSA SSPNMNVYNQ QIL TQQPQQ QLQQQQLHQP QPQQPQPARQ SPHLLIHQQQ QQQQQQQQQQ QQQQQHPVTH RPSNVSQTMP QPSQPLQSST PSSA VGRNQ SPGATPVTSV NAAAKPSPGT SSTSTLINQH ILKPAPPPTE IPARLQVKPP QPAAMKIPNR PTLLGGSAIS QPSLT TPVS IRPPPLEMEG DHVLQKRKLK ELLRNVGADE GDGETVIDGD VEELLLDLAD EFVTSVTSFA CRLAKHRKVD NIDMRD VQL HLERNWNIRV PGYASDEIRS VRKFQPTAGY NQKVQGVAIS KSVNKN

UniProtKB: Transcription initiation factor TFIID subunit 12

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分子 #9: Subunit (90 kDa) of TFIID and SAGA complexes

分子名称: Subunit (90 kDa) of TFIID and SAGA complexes / タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 80.933008 KDa
配列文字列: MKQEPGDSSD KSAPSGSITP QPKPPQQANV SNQQPQKPQQ FSAADLNRIV LEYLNKKGYH KTESMLRMES SHIPAPPTNI PTPQTTNIS RPTAPGRAPE YSDDPATIKR GYSILKSWCE SSLDFYRPEL EKFLYPVFVH CYLDLIARGY PSHAREFYDK F SKDHSVLH ...文字列:
MKQEPGDSSD KSAPSGSITP QPKPPQQANV SNQQPQKPQQ FSAADLNRIV LEYLNKKGYH KTESMLRMES SHIPAPPTNI PTPQTTNIS RPTAPGRAPE YSDDPATIKR GYSILKSWCE SSLDFYRPEL EKFLYPVFVH CYLDLIARGY PSHAREFYDK F SKDHSVLH EYEISKLGGI SLKEHLQEND VAKIFRSHKF KVLIGRTTFN LLLYFLNEND AVGGGVVLRL INQYIEPVIT TE AIAVERE GELNLSEGIV ELHTLNNTSI GGEQREISSV DAFNKKPVKL GKLQVDPEYS KELEAELKLK DEHEQAAQKK VST TLLEEY RENFKVDPSD ENNPSKDTLP LPLKSAQDLR NDIAMIQDSR AKIKLSAAQA SLPSVCMYTF HNTNNDLTCL KFND DSTMV ASGFQDSFIK LWSIDGSPLR SLLKNDPYNQ QNNDGVAVKG SRRLVGHSGA VYGVDFSPDN RYLISCSEDK TVRLW SLDT YTCLVSYKGH SSSVWDVKFS PMGHYFATAS HDQTARLWSC DHIYPLRIFA GHLNDVDCVE FHPNSTYLFT GSSDKT ARM WDIARGECVR VFMGHSGAIN CLAVSPDGRW LASAGEDSVV CLWDISTGRR IKAMRGHGRS SIYSLAFSRE GTVLVST GA DNSVRVWDVK KNTNSPSAQP EPINDVTAQG IQKKTEDLRR RKEIVATNDH MSVYFTKKTP VYTVHFTRRN LCLAGGVF G G

UniProtKB: Transcription initiation factor TFIID subunit 5

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分子 #10: Subunit (60 kDa) of TFIID and SAGA complexes

分子名称: Subunit (60 kDa) of TFIID and SAGA complexes / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 54.888445 KDa
配列文字列: MSKNSQRSSI PTSHTLWSPS DTVKDAAESL GIFNLNEEAA KNLAMDIEYR IHEILDQASK FMRHGKRRTL HTSDIDRALK VLNLEPLYG YDVSRPLVFK EALVGAGQNL YYVDDDEVDF EKLINEPLPK VPRFSTFTAH WLAIEGVQPA IPQNPSPNDI K NILPINRG ...文字列:
MSKNSQRSSI PTSHTLWSPS DTVKDAAESL GIFNLNEEAA KNLAMDIEYR IHEILDQASK FMRHGKRRTL HTSDIDRALK VLNLEPLYG YDVSRPLVFK EALVGAGQNL YYVDDDEVDF EKLINEPLPK VPRFSTFTAH WLAIEGVQPA IPQNPSPNDI K NILPINRG SMENMFSLIN DEVKEDTNEE FTSTGPSVSS NISNQKQGLE VKPLVKHVLS RELQLYFDKI VEVLLNQEET KE AELLRNS ALQSVRADPG LHQLVPYFIQ FISETITKNL KNISLLSTML ELIYSLLMNE SLFLEPYVHA IIPCILTLLL AKK IGNVDD ELQKQQQLAL RELSASLLER VIEDFGSSYS TLKPRITRTL LRAFVSVNNT TPGTQYGALL GLRGLGSEVI RIVV LGNVI NWSSTFLEKL QQEDQVFLID TLIETLRVLT KEGKLVKDMK TENGIDNERL KQRVGDLIAD RIQACDDAQD IYWGI FFGE V

UniProtKB: Subunit (60 kDa) of TFIID and SAGA complexes

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分子 #11: Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA pol...

分子名称: Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation
タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 17.303576 KDa
配列文字列:
MTNEQAAIPR DVRLLHLIFA TQNIYSYQDH VPLQLMDFAY RYTTGTLQDA TIYSDHAHAS GSHISNAGNA GTNAQLTTED IRLAIAART NYQFKPVPPK ELLLELAAER NKKPLPAVIP TWGIRLPPEK YCLTGKDWVL EDEEEAVSYK KRKT

UniProtKB: Transcription initiation factor TFIID subunit 9

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分子 #12: Transcription-associated protein

分子名称: Transcription-associated protein / タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 438.055344 KDa
配列文字列: MLHVVQLDDF ATRLKAAEDY QSKHSVLSEI CDSLETFNAA QDYEYFLKSL IPLFIDVLKE VPVSFVANSP ENKLRNITLE ILHRIPAND ALQAYSNEIV DTLMDLLKVE NELNGILCMK AITTLHKTFK ASLQEKVHPF IDIVIEIYSN IPQVVEEQFN G NQIDSKEN ...文字列:
MLHVVQLDDF ATRLKAAEDY QSKHSVLSEI CDSLETFNAA QDYEYFLKSL IPLFIDVLKE VPVSFVANSP ENKLRNITLE ILHRIPAND ALQAYSNEIV DTLMDLLKVE NELNGILCMK AITTLHKTFK ASLQEKVHPF IDIVIEIYSN IPQVVEEQFN G NQIDSKEN VDSTSRPNSP SFSSQSDDSK QLAQAMFSFK TLAESPITMV SLYSSYKELA ASSLGNFIPH VMKVLSLEVA KQ AEARKAA EEKGIILVNV CKEITNRANY GEFIIGQVKA ASFLAYLFIR RQAQTFLEPY QQAIPDIIIR LLQDCPSELS AAR KELLHA TRHILSTDFR KMFIPKIDLL FDLRVLIGEG FTAYETLRPL AYSTVADFIH NVRDHLTPAQ LWKSVSIYCK NLQD DSLAL TVQIMSAKLL LNLIEKIMRS ESKTESRQLL MVIIDAYTKR FKMLNSRYNG IMKQHATYEK EKQEKQNQER LLTNK LDGT TPSPSDDKKV ELIDEDQDVK MEDPTPEISD QETIKGDNDA STEPQDSEQQ LADFMSLQEY LPIQVSVPPE IDLLKD SRY LFKTLMTFLK TIMIGLKNSN PPSSQNHFNA QNWNETARGF SNEDINILKS LFRECILALR FFSTSKTSLP ASSMKQS FD ITGPNLPITS TKEEKDLMEI FATMFIHIDP ASFNEIVREE LPFMYKQMLD FASLLHIPQF FLASVITSSS FSGILITF L KSKLVDLGEV NIIKSNILIR LFKLCFMSVS LFPAANESVI LPHLNELILK SLKLSTTAKE PLVYFYLIRT LFRSIGGGR FENLYKEIMP LLQVLLESLS KLIHEARRPQ ERDIYVELCL TVPVRLSVLV PHLSYLMKPL VYALNGSQES VSQGLRTLEL CVDNLTAEY FDPIIEPVID DVMEALSKHL KPLPYYHQHS HTTLRILGKL GGRNRTFIKP VDNLKTDSEL FQNVEAMFKI H GLPNEVPL SITPGLSAAF SLLTDPRPRI HYRINSFKYI SGIFQLFLGA TQLPDDYANR LKESMDIILE DTIAPDEPLN KL HHFPVKD IAKYDSQMEL LVKLLESIFY AVSLQEVREE SKALIRGTCN HFILLYFNKM VIDKRKFVRK FSVDNHEGNL FLN ENCIFD AIIYALSSDN SAVRSMGLES VQLIYDSCVE LFGNIDCALK FAPLNVMCSK FIHCCFEEPY HKKLAGCIGL EMML NSLDI PMKYFNARQL EIIRALFYVL RDTAPELPCE VTNTAKRLIL NSLKEWNKEL TRNDVFSSVF QNLVSSLIVD LPNAN EIVR ATAQEALRTL SETTQVPIAT MISPCKHILL APIFGKPLRA LPFQMQIGNI DAITFCMGLE NSFLEYNEEL NRLVQE ALA LVDAEDESLV SAHRISEHKT SEQLVRLRVV CIQLLSLAIT KPEFAAAQQR SNIRVKILVV FFKSLCGRSI EIIRAAH GG LKAVIDLKMK LPKELLQNGL RPMLMNLSDH KKLTVASLEA LSGLLKLFIS YFKVGIGSKL LDHLLAWAQP RTLQQLGS Q DLENNSTVQI IVAILDVFHL LPPTAHKFMN DLMNALLYLE NNLHRCQYSP FREPLAKFLD RFPDESFEYF FNEFSKREI TTRFVYFVGL DSCSSLRAKV LESLPRVRGL LHQEGSAEEK CVRFSNLVDL CESLAASDKE WIKDKEELLG ELLDAGSVCL TLKRSSNVV SPLYFQVDQG FETLQLLYIE YFKSQPLGHE KVFNFIDKIS KEGLPFVLEF DDFIFNEVVK CQDIPTVQQT L DTIIRMTP QVSSLDARVY LYKRIFLPIC IYESEMHGDL SRLSQTENNE LPAWLKSFDS DVWKATGPLV DDYTSTLEDR YR LELMQLT ALLIKGAPTA LTDMRKDIIK FSWNYIKLDD NTSKQAAYVV TAYFISRFDT PSELTTRIFV ALLRCHQIDT RYL VKQALE LLAPVLSERT NSELDWLKWP RRVLSEDGFN ITQVANIYQL IVKFPDLFYP ARDHFIPNII TAMGKLTVMS NTSL ENQQL AIDLAELILK WETKLPKSEK LGSAEETEKE KSVSEDKMDI DVKEETKEDI AERPKAEDQI GGDDSDSSNI LTSED YEVS FAQREACVTF LIRYICISTQ RPSENELGKR ALNILYELLG PKYWSEVTVK LQFFERFLMS SDLNQPSLLG YCLNAL EVL AVALKWKPTT WIIENVSYLQ KLLEKCLRSD NQDIQEILQK VLGIILEAIN KETQGSEEDE PEEVTNFISL IVNIIGE DL SNMTSVAAGV SLCWTLSLYR PNALDSLLPS IMRTFNKLCR DHIAISLQGN QPQSGDFANI EFEAKVTTNL LEKILNLC A ARISSLDDQR RVFLSLLAQL IDRSVDKDML LKVINIVTEW IFKTDFYPTT KEKAGILGKM MIFDLRGEPE LSKKFNQVI VDIFESKELA HTELTARMET AFLFGTRLSD VSIRKKLMSI LSDSLELDID KRLFYIIKDQ NWEYLSDYPW LNQALQLLYG SFHLDSPIR LSPEENTLSP LQSITEGLAR EKSPVEKAPQ NIIDFVAKHN EFLDSVRSLT AGDILNPLID ISYQSAETIH N AWVVVFPV AYSAIESRYE LEFTRALVKL LFKDYHIRQQ DARPNVIKSL LDGVGKCPGL HLPPHLVKYL GSNYNAWYGA IK LLEELSE GQGIDNQKIS DANQDALLEV YMSLQEDDMF YGTWRRRAKY FETNAALSYE QIGIWDKALQ LYEAAQIKAR SGV FPFGES EYSLWEDHWI YCAEKLQHWE ILTELAKHEG FTDLLLECGW RGADWIADRE PLEQSVKTVM DIPTPRRQIF QTFL ALQGF SQQKDTLQDV SRLCDEGIQL TLRKWNALPQ RVTRAHIGLL HTFQQYVELM EASQVYSSLV TTNAQNLDVK SQELK RVLQ AWRERLPNVW DDINIWNDLV TWRQHVFGVI NRVYMPFVPV LQQSNGTNNG NSYAYRGYHE MAWVINRFAH VARKHE MPE VCINQLTKIY TLPNIEIQEA FLKLREQAKC HYQNSSELNT GLDVISNTNL VYFATQQKAE FFTLKGMFLA KLNAKDE AN QAFATAVQID LNLPKAWAEW GFFNDRRFKE NPEEIFHAKN AISCYLQAAG LYKDGKTRKL LCRILWLISL DDAAGSLA K TFEDHHGESP VWYWITFVPQ LLTSLSHKEA KIVRHILIQI AKSYPQSLHF QLRTTKEDYQ AIQRQAMAVN RAEEQSSNK QDTADSVLKN TNTPQPQTRT ETSGTTAESD KKPSIPPKEE QGSPQPSRPA TTQASPQAQS QENGESSQKH PPEIPTTDSR QPWQDVEEI MGILKTAYPL LALSLESLVD QLNQRFKCNA DEDAYRLVIV LYNDGVQQMN RVANPREEVK LPAATEASIS R FADSVLPK NIREVFEQDI IACNPNLETY ISKLRKWRDC LEEKLDRSYG KADLERVSLH LSLFHHQKFE DIEIPGQYLL HK DNNNHFI KIERFLPTLD LVRGSNGCYK RMTIRGNDGS LHPFAVQFPA ARHCRREERI FQLFRIFDDA LSRKVQSRRR NIS LTLPIA VPLSPHIRIL NDDKRYTTLM GIYEEFCRRK GQSRDEPFAY TIQKLRAAFD PRLPKPDIVS VRAEVLASIQ STLV PSTLL KDYYTEKFSN YENYWLFRKQ FTAQYASFIF MTYIMCINSR QPQKIHINEG SGNIWTSEML PTKVATGKTH STAYN NSTL DPAVKAGAPI FYNTESVPFR LTPNIQKFIG EAGLEGILSV YILVIANSLS DSEFDMEQYL SLFVRDEVIS WFAQQH RAS AQTNQLREIV RVNVELLTKR VLQLNHIPNS QNVATQFVLN LISQAVNPRN LAYTDSAWMA YL

UniProtKB: Transcription-associated protein

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分子 #13: Transcriptional regulator involved in glucose repression of Gal4p...

分子名称: Transcriptional regulator involved in glucose repression of Gal4p-regulated genes
タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 81.864305 KDa
配列文字列: MAARSSTRRG GQPPGVAKSL GSNKSSPVKK GLGAGKGKPK VDYPKQNPSS TLHDILSELN LSYDSNLGLL QGDGIAFPPS VSVLNNVKG LFEQLIEELQ YVSNYDDNLL KIIDNKLKEI EHKPSTGEVK EEEIDGTESE QPARKRQKVQ STDRIEIPDT K PQGLPLKE ...文字列:
MAARSSTRRG GQPPGVAKSL GSNKSSPVKK GLGAGKGKPK VDYPKQNPSS TLHDILSELN LSYDSNLGLL QGDGIAFPPS VSVLNNVKG LFEQLIEELQ YVSNYDDNLL KIIDNKLKEI EHKPSTGEVK EEEIDGTESE QPARKRQKVQ STDRIEIPDT K PQGLPLKE SSSQKVDSKT SVKDEAKEDE IGDDNDDDDE DDAMEEDEAG DDDEDEEEAE IEGEAGIGGE PGAEAAVEAD DD EEDEEVE EEEAASEAKP VVKEGFEQEG HYTRENDTRV KNPKSEFVTS QTLPVAAVEL GLYDENGLEQ TGEDYYKRKY GVA SYPTSD LKEFLPGELP DEDFTKSKPP SQVQFSTFQS YLEPYFRPFT EEDIKFLKKQ YNLPQNLPKN YDPNVTPFVI PKFG AHYRD TWYEEDINTG MIKKKPNDVD PRLADQKMRQ DALIPKGSGA SLTDDVLETE KVSCGPLASR LLSALLKNGD DTEDG EPVD NDDNNNVSSA IGNEGWTPSV ETSDYETLDQ RLRRELKYIG VFMNVEQTLK REGHADELVN SVGVTSGNGE FEEDWL NSR EDDEISAEMR SLQRELRRLT RKNVRRKEKL IPIVEEQLAW QEYQSILEDL DKQVDQAYLR RIRVPRKNKR KNESQSS GQ QTQQQQQQQQ QQQQQDQQQT GFKALLDKRT RWIEKIGPLF KPPHLMRRPP SESVFKDVDL ENDDEELEED DEMKVDID L R

UniProtKB: Transcriptional regulator involved in glucose repression of Gal4p-regulated genes

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分子 #14: Spt8

分子名称: Spt8 / タイプ: protein_or_peptide / ID: 14 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii GS115 (菌類) / 株: GS115 / ATCC 20864
分子量理論値: 34.059902 KDa
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

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分子 #15: Polyubiquitin-B

分子名称: Polyubiquitin-B / タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 8.560831 KDa
組換発現生物種: Escherichia coli (大腸菌)
配列文字列:
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRG(GLZ)

UniProtKB: Ubiquitin B

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分子 #16: Ubiquitin carboxyl-terminal hydrolase

分子名称: Ubiquitin carboxyl-terminal hydrolase / タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO / EC番号: ubiquitinyl hydrolase 1
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 56.745879 KDa
配列文字列: MTSTVKKTLE EAKFSPPVFD SCPHLKQILT SNAKDTVYRN YGLAVNVALA LKLRNGNIPL YKTSDGSKVE YKKLARLRNK AVRCRECLE SLSCTYFCLQ CSHVGCWKNG HASAHTKSTG HVFGIDSQTG YIFCFRCGDY VGDQRLEFIR QSKQGERTEP I MNNLGTET ...文字列:
MTSTVKKTLE EAKFSPPVFD SCPHLKQILT SNAKDTVYRN YGLAVNVALA LKLRNGNIPL YKTSDGSKVE YKKLARLRNK AVRCRECLE SLSCTYFCLQ CSHVGCWKNG HASAHTKSTG HVFGIDSQTG YIFCFRCGDY VGDQRLEFIR QSKQGERTEP I MNNLGTET IIDGSRPPSL KASTGLRGFI NMGATCFMSS IVQTIVHNPF VRDYFLSGYH AKCTKKMDTC ITCCIDEIFK VF YGTDETK GYGPTALLTA AWRVKNSLAG YSEQDAHEFW QFLLDEIHKS DTELHPELDD TSTCRCITHK TFAGQLQSTV TCE KCLHSK NTVDPMLDLS LEIAKISKDK VTLNDCLDLF TSKEKLDSMT ICSHCKKETT RTKQLLVRKL PPILAFQLKR FEHS ASSST KIETHVGFPL FLDMEPYTVS ETLNSNEQGR ASWGLMTYQL FAIVIHIGSV NTGHYICIIK NRDGNWFKFD DSRIT LVSQ DYVSKSNAYL LYYILCQA

UniProtKB: Ubiquitin carboxyl-terminal hydrolase

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分子 #17: SAGA-associated factor 11

分子名称: SAGA-associated factor 11 / タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 13.977742 KDa
配列文字列:
MSSEVMQETI WDCLMSNILR QIVINNILQE QALRSSIKAI SNDDQISLKE INAQRLKFVK DDKDIFNHIN GKRSENKYNG TGADASNGT TDTEYFTCLN CDRKIAGNRF ASHVDRCLGG RTRK

UniProtKB: SAGA-associated factor 11

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分子 #18: Transcription and mRNA export factor SUS1

分子名称: Transcription and mRNA export factor SUS1 / タイプ: protein_or_peptide / ID: 18 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (菌類)
株: GS115 / ATCC 20864
分子量理論値: 11.126675 KDa
配列文字列:
MTNSDVQHLK QQIQDVLVES GKYDELSNFL RSKLYQVGWT DEINRLTQSI VTNEAKPTFS NVIERIEPKA MDIVPEHIKT EILAKIEEF LKDVVPK

UniProtKB: Transcription and mRNA export factor SUS1

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分子 #19: water

分子名称: water / タイプ: ligand / ID: 19 / コピー数: 91 / 式: HOH
分子量理論値: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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実験情報

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構造解析

手法クライオ電子顕微鏡法
解析単粒子再構成法
試料の集合状態particle

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試料調製

濃度0.4 mg/mL
緩衝液pH: 8
凍結凍結剤: ETHANE / チャンバー内湿度: 95 % / チャンバー内温度: 283 K / 装置: FEI VITROBOT MARK IV

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電子顕微鏡法

顕微鏡FEI TITAN KRIOS
特殊光学系エネルギーフィルター - 名称: GIF Quantum LS / エネルギーフィルター - スリット幅: 20 eV
撮影フィルム・検出器のモデル: GATAN K2 QUANTUM (4k x 4k)
検出モード: SUPER-RESOLUTION / 平均露光時間: 8.0 sec. / 平均電子線量: 52.8 e/Å2
電子線加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
電子光学系C2レンズ絞り径: 70.0 µm / 最大 デフォーカス（補正後）: 4.5 µm / 最小 デフォーカス（補正後）: 0.8 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 0.01 mm / 最大 デフォーカス（公称値）: 3.5 µm / 最小 デフォーカス（公称値）: 1.5 µm / 倍率（公称値）: 105000
試料ステージ試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER
ホルダー冷却材: NITROGEN
実験機器
モデル: Titan Krios / 画像提供: FEI Company

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画像解析

粒子像選択選択した数: 1068534
初期モデルモデルのタイプ: EMDB MAP
最終 再構成想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 20.0 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 3.0) / 使用した粒子像数: 354104
初期 角度割当タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 3.0)
最終 角度割当タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 3.0)

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原子モデル構築 1

精密化プロトコル: OTHER
得られたモデル

PDB-6tbm:
Structure of SAGA bound to TBP, including Spt8 and DUB

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る