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TitleStructure of SAGA and mechanism of TBP deposition on gene promoters.
Journal, issue, pagesNature, Vol. 577, Issue 7792, Page 711-716, Year 2020
Publish dateJan 22, 2020
AuthorsGabor Papai / Alexandre Frechard / Olga Kolesnikova / Corinne Crucifix / Patrick Schultz / Adam Ben-Shem /
PubMed AbstractSAGA (Spt-Ada-Gcn5-acetyltransferase) is a 19-subunit complex that stimulates transcription via two chromatin-modifying enzymatic modules and by delivering the TATA box binding protein (TBP) to ...SAGA (Spt-Ada-Gcn5-acetyltransferase) is a 19-subunit complex that stimulates transcription via two chromatin-modifying enzymatic modules and by delivering the TATA box binding protein (TBP) to nucleate the pre-initiation complex on DNA, a pivotal event in the expression of protein-encoding genes. Here we present the structure of yeast SAGA with bound TBP. The core of the complex is resolved at 3.5 Å resolution (0.143 Fourier shell correlation). The structure reveals the intricate network of interactions that coordinate the different functional domains of SAGA and resolves an octamer of histone-fold domains at the core of SAGA. This deformed octamer deviates considerably from the symmetrical analogue in the nucleosome and is precisely tuned to establish a peripheral site for TBP, where steric hindrance represses binding of spurious DNA. Complementary biochemical analysis points to a mechanism for TBP delivery and release from SAGA that requires transcription factor IIA and whose efficiency correlates with the affinity of DNA to TBP. We provide the foundations for understanding the specific delivery of TBP to gene promoters and the multiple roles of SAGA in regulating gene expression.
External linksNature / PubMed:31969704
MethodsEM (single particle)
Resolution3.5 - 20.0 Å
Structure data

10438

6tb4

EMDB-10438, PDB-6tb4:
Structure of SAGA bound to TBP
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-10440:
SAGA Tra1 lobe locally refined
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-10441:
SAGA Main lobe locally refined
Method: EM (single particle) / Resolution: 3.5 Å

10446

6tbm

EMDB-10446: SAGA-TBP filtered to show the position of the DUB domain
PDB-6tbm: Structure of SAGA bound to TBP, including Spt8 and DUB
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-10447:
Transcription co-activator complex SAGA
Method: EM (single particle) / Resolution: 5.92 Å

EMDB-10448:
Focused 3D classification to reveal the position of TBP bound to SAGA
Method: EM (single particle) / Resolution: 8.2 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • komagataella phaffii gs115 (fungus)
  • komagataella phaffii (strain gs115 / atcc 20864) (fungus)
  • Yeast (fungus)
  • Komagataella phaffii (fungus)
  • homo sapiens (human)
KeywordsTRANSCRIPTION / Transcriptional co-activator / Histone-acetylation

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