+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-0729 | |||||||||
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タイトル | Structure of human cardiac thin filament in the calcium bound state | |||||||||
マップデータ | ||||||||||
試料 |
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キーワード | Troponin / Tropomyosin / Actin / Thin filement / Muscle / CONTRACTILE PROTEIN-ACTIN BINDING PROTEIN complex | |||||||||
機能・相同性 | 機能・相同性情報 positive regulation of heart rate by epinephrine / muscle thin filament tropomyosin / regulation of systemic arterial blood pressure by ischemic conditions / troponin C binding / regulation of muscle filament sliding speed / troponin T binding / diaphragm contraction / regulation of ATP-dependent activity / cardiac Troponin complex / cardiac myofibril ...positive regulation of heart rate by epinephrine / muscle thin filament tropomyosin / regulation of systemic arterial blood pressure by ischemic conditions / troponin C binding / regulation of muscle filament sliding speed / troponin T binding / diaphragm contraction / regulation of ATP-dependent activity / cardiac Troponin complex / cardiac myofibril / troponin complex / bleb / negative regulation of vascular associated smooth muscle cell migration / transition between fast and slow fiber / regulation of smooth muscle contraction / regulation of muscle contraction / muscle filament sliding / negative regulation of ATP-dependent activity / ruffle organization / regulation of cardiac muscle contraction by calcium ion signaling / positive regulation of ATP-dependent activity / Striated Muscle Contraction / response to metal ion / structural constituent of muscle / ventricular cardiac muscle tissue morphogenesis / cytoskeletal motor activator activity / sarcomere organization / regulation of heart contraction / tropomyosin binding / heart contraction / myosin heavy chain binding / mesenchyme migration / troponin I binding / filamentous actin / actin filament bundle / negative regulation of vascular associated smooth muscle cell proliferation / skeletal muscle thin filament assembly / actin filament bundle assembly / striated muscle thin filament / skeletal muscle contraction / skeletal muscle myofibril / actin monomer binding / Smooth Muscle Contraction / vasculogenesis / calcium channel inhibitor activity / skeletal muscle fiber development / stress fiber / Ion homeostasis / cardiac muscle contraction / titin binding / cytoskeleton organization / positive regulation of stress fiber assembly / cytoskeletal protein binding / actin filament polymerization / sarcomere / positive regulation of cell adhesion / negative regulation of cell migration / actin filament organization / filopodium / actin filament / 加水分解酵素; 酸無水物に作用; 酸無水物に作用・細胞または細胞小器官の運動に関与 / wound healing / structural constituent of cytoskeleton / ruffle membrane / intracellular calcium ion homeostasis / cellular response to reactive oxygen species / response to calcium ion / calcium-dependent protein binding / actin filament binding / actin cytoskeleton / lamellipodium / heart development / regulation of cell shape / actin binding / cell body / cytoskeleton / hydrolase activity / protein heterodimerization activity / protein domain specific binding / calcium ion binding / positive regulation of gene expression / protein kinase binding / magnesium ion binding / protein homodimerization activity / ATP binding / identical protein binding / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | Oryctolagus cuniculus (ウサギ) / Homo sapiens (ヒト) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.8 Å | |||||||||
データ登録者 | Fujii T / Yamada Y | |||||||||
引用 | ジャーナル: Nat Commun / 年: 2020 タイトル: Cardiac muscle thin filament structures reveal calcium regulatory mechanism. 著者: Yurika Yamada / Keiichi Namba / Takashi Fujii / 要旨: Contraction of striated muscles is driven by cyclic interactions of myosin head projecting from the thick filament with actin filament and is regulated by Ca released from sarcoplasmic reticulum. ...Contraction of striated muscles is driven by cyclic interactions of myosin head projecting from the thick filament with actin filament and is regulated by Ca released from sarcoplasmic reticulum. Muscle thin filament consists of actin, tropomyosin and troponin, and Ca binding to troponin triggers conformational changes of troponin and tropomyosin to allow actin-myosin interactions. However, the structural changes involved in this regulatory mechanism remain unknown. Here we report the structures of human cardiac muscle thin filament in the absence and presence of Ca by electron cryomicroscopy. Molecular models in the two states built based on available crystal structures reveal the structures of a C-terminal region of troponin I and an N-terminal region of troponin T in complex with the head-to-tail junction of tropomyosin together with the troponin core on actin filament. Structural changes of the thin filament upon Ca binding now reveal the mechanism of Ca regulation of muscle contraction. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_0729.map.gz | 23.3 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-0729-v30.xml emd-0729.xml | 16.1 KB 16.1 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_0729_fsc.xml | 7.1 KB | 表示 | FSCデータファイル |
画像 | emd_0729.png | 45.6 KB | ||
Filedesc metadata | emd-0729.cif.gz | 5.9 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-0729 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-0729 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_0729_validation.pdf.gz | 498 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_0729_full_validation.pdf.gz | 497.6 KB | 表示 | |
XML形式データ | emd_0729_validation.xml.gz | 9.6 KB | 表示 | |
CIF形式データ | emd_0729_validation.cif.gz | 12.4 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-0729 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-0729 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_0729.map.gz / 形式: CCP4 / 大きさ: 30.5 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 2.22 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
+全体 : Cardiac muscle thin filament in the calcium bound state
+超分子 #1: Cardiac muscle thin filament in the calcium bound state
+超分子 #2: Actin, alpha skeletal muscle
+超分子 #3: Tropomyosin, Troponin
+分子 #1: Actin, alpha skeletal muscle
+分子 #2: Tropomyosin alpha-1 chain
+分子 #3: Tropomyosin alpha-1 chain
+分子 #4: Troponin T, cardiac muscle
+分子 #5: Troponin I, cardiac muscle
+分子 #6: Troponin C, slow skeletal and cardiac muscles
+分子 #7: ADENOSINE-5'-DIPHOSPHATE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | filament |
-試料調製
濃度 | 0.05 mg/mL |
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緩衝液 | pH: 7.5 |
凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | JEOL CRYO ARM 200 |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 65.0 e/Å2 |
電子線 | 加速電圧: 200 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |