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- EMDB-9222: Cryo-EM structures and dynamics of substrate-engaged human 26S pr... -

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Basic information

Entry
Database: EMDB / ID: EMD-9222
TitleCryo-EM structures and dynamics of substrate-engaged human 26S proteasome
Map dataThe complete map of state ED2 of substrate-engaged human proteasome, low pass-filtered to 3 Angstrom without amplitude correction
Sample
  • Complex: Proteasome
    • Protein or peptide: x 29 types
  • Protein or peptide: x 4 types
  • Ligand: x 4 types
KeywordsProteosome / HYDROLASE
Function / homology
Function and homology information


positive regulation of inclusion body assembly / thyrotropin-releasing hormone receptor binding / nuclear proteasome complex / host-mediated perturbation of viral transcription / Impaired BRCA2 translocation to the nucleus / Impaired BRCA2 binding to SEM1 (DSS1) / proteasome accessory complex / purine ribonucleoside triphosphate binding / integrator complex / cytosolic proteasome complex ...positive regulation of inclusion body assembly / thyrotropin-releasing hormone receptor binding / nuclear proteasome complex / host-mediated perturbation of viral transcription / Impaired BRCA2 translocation to the nucleus / Impaired BRCA2 binding to SEM1 (DSS1) / proteasome accessory complex / purine ribonucleoside triphosphate binding / integrator complex / cytosolic proteasome complex / proteasome regulatory particle / structural constituent of proteasome / positive regulation of proteasomal protein catabolic process / transcription factor binding / CD8-positive, alpha-beta T cell differentiation / CD8-positive, alpha-beta T cell homeostasis / thymic T cell selection / Antigen processing: Ub, ATP-independent proteasomal degradation / transcription export complex 2 / proteasome-activating activity / spermatoproteasome complex / proteasome regulatory particle, lid subcomplex / negative regulation of programmed cell death / negative regulation of regulatory T cell differentiation / T-helper 1 cell differentiation / metal-dependent deubiquitinase activity / Regulation of ornithine decarboxylase (ODC) / proteasome core complex / Proteasome assembly / cellular response to type I interferon / T-helper 17 cell differentiation / Cross-presentation of soluble exogenous antigens (endosomes) / retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum / protein K63-linked deubiquitination / Somitogenesis / K63-linked deubiquitinase activity / Homologous DNA Pairing and Strand Exchange / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / flagellated sperm motility / Resolution of D-loop Structures through Holliday Junction Intermediates / proteasome binding / sperm end piece / Impaired BRCA2 binding to RAD51 / myofibril / immune system process / proteasomal ubiquitin-independent protein catabolic process / positive regulation of RNA polymerase II transcription preinitiation complex assembly / general transcription initiation factor binding / : / ciliary tip / proteasome storage granule / Presynaptic phase of homologous DNA pairing and strand exchange / protein deubiquitination / proteasome endopeptidase complex / NF-kappaB binding / proteasome core complex, beta-subunit complex / endopeptidase activator activity / threonine-type endopeptidase activity / proteasome core complex, alpha-subunit complex / proteasome assembly / mRNA export from nucleus / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / proteasome complex / enzyme regulator activity / regulation of G1/S transition of mitotic cell cycle / regulation of macroautophagy / stem cell differentiation / positive regulation of interleukin-2 production / ERAD pathway / response to type II interferon / inclusion body / Activation of STAT3 by cadherin engagement / TBP-class protein binding / regulation of proteasomal protein catabolic process / meiotic cell cycle / sarcomere / Regulation of activated PAK-2p34 by proteasome mediated degradation / ubiquitin binding / negative regulation of inflammatory response to antigenic stimulus / proteasomal protein catabolic process / Autodegradation of Cdh1 by Cdh1:APC/C / APC/C:Cdc20 mediated degradation of Securin / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / lipopolysaccharide binding / protein catabolic process / Asymmetric localization of PCP proteins / Ubiquitin-dependent degradation of Cyclin D / SCF-beta-TrCP mediated degradation of Emi1 / AUF1 (hnRNP D0) binds and destabilizes mRNA / NIK-->noncanonical NF-kB signaling / positive regulation of type II interferon production / TNFR2 non-canonical NF-kB pathway / P-body / Assembly of the pre-replicative complex / Vpu mediated degradation of CD4 / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / Dectin-1 mediated noncanonical NF-kB signaling
Similarity search - Function
: / Ubiquitin interaction motif / : / : / 26S proteasome regulatory subunit RPN7/PSMD6 C-terminal helix / PSMD3-like, N-terminal TPR repeats / 26S proteasome non-ATPase regulatory subunit Rpn12 / 26S proteasome regulatory subunit, C-terminal / Proteasome regulatory subunit C-terminal / 26S proteasome regulatory subunit RPN5, C-terminal domain ...: / Ubiquitin interaction motif / : / : / 26S proteasome regulatory subunit RPN7/PSMD6 C-terminal helix / PSMD3-like, N-terminal TPR repeats / 26S proteasome non-ATPase regulatory subunit Rpn12 / 26S proteasome regulatory subunit, C-terminal / Proteasome regulatory subunit C-terminal / 26S proteasome regulatory subunit RPN5, C-terminal domain / : / 26S proteasome regulatory subunit RPN5 C-terminal domain / PSD13 N-terminal repeats / 26S proteasome regulatory subunit Rpn6, N-terminal / 6S proteasome subunit Rpn6, C-terminal helix domain / 26S proteasome regulatory subunit RPN6 N-terminal domain / 26S proteasome subunit RPN6 C-terminal helix domain / 26S Proteasome non-ATPase regulatory subunit 13 / Proteasome subunit Rpn10 / 26S Proteasome non-ATPase regulatory subunit 7/8 / : / 26S proteasome subunit RPN2, N-terminal domain / 26S proteasome regulatory complex, non-ATPase subcomplex, Rpn2/Psmd1 subunit / 26S proteasome regulatory subunit RPN2, C-terminal / 26S proteasome regulatory subunit RPN2 C-terminal domain / : / 26S proteasome regulatory subunit RPN11 C-terminal domain / 26S proteasome regulatory complex, non-ATPase subcomplex, Rpn1 subunit / RPN1, N-terminal / 26S proteasome non-ATPase regulatory subunit RPN1, C-terminal / RPN1 N-terminal domain / 26S proteasome non-ATPase regulatory subunit RPN1 C-terminal / DSS1/SEM1 / DSS1/SEM1 family / DSS1_SEM1 / Proteasome/cyclosome repeat / Ubiquitin-interacting motif. / Proteasome/cyclosome repeat / : / 26S proteasome regulatory subunit 7, OB domain / von Willebrand factor type A domain / : / : / : / PSMD12/CSN4, N-terminal / 26S proteasome regulatory subunit Rpn7/COP9 signalosome complex subunit 1 / 26S proteasome regulatory subunit Rpn7, N-terminal / 26S proteasome subunit RPN7 / 26S Proteasome non-ATPase regulatory subunit 12/COP9 signalosome complex subunit 4 / : / PCI/PINT associated module / HEAT repeats / Proteasomal ATPase OB C-terminal domain / Proteasomal ATPase OB C-terminal domain / Proteasome subunit alpha 1 / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / Rpn11/EIF3F, C-terminal / Maintenance of mitochondrial structure and function / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / : / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / Ubiquitin interacting motif / Ubiquitin-interacting motif (UIM) domain profile. / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / Proteasome beta subunit, C-terminal / Proteasome beta subunits C terminal / Proteasome subunit beta 4 / Proteasome subunit beta 2 / Proteasome beta 3 subunit / Proteasome subunit alpha5 / Proteasome subunit alpha6 / Proteasome beta-type subunits signature. / Peptidase T1A, proteasome beta-subunit / Proteasome beta-type subunit, conserved site / Proteasome subunit A N-terminal signature / Proteasome alpha-type subunits signature. / Proteasome alpha-subunit, N-terminal domain / Proteasome subunit A N-terminal signature Add an annotation / Proteasome B-type subunit / Proteasome beta-type subunit profile. / Proteasome alpha-type subunit / Proteasome alpha-type subunit profile. / VWFA domain profile. / Proteasome subunit / Proteasome, subunit alpha/beta / von Willebrand factor (vWF) type A domain / von Willebrand factor, type A / JAB/MPN domain / JAB1/MPN/MOV34 metalloenzyme domain / AAA ATPase, AAA+ lid domain / AAA+ lid domain / MPN domain / MPN domain profile. / ATPase, AAA-type, conserved site / AAA-protein family signature. / Nucleophile aminohydrolases, N-terminal / von Willebrand factor A-like domain superfamily
Similarity search - Domain/homology
26S proteasome regulatory subunit 10B / 26S proteasome non-ATPase regulatory subunit 11 / 26S proteasome non-ATPase regulatory subunit 12 / Ubiquitin C-terminal hydrolase PSMD14 / Proteasome subunit alpha type-7 / 26S proteasome non-ATPase regulatory subunit 3 / 26S proteasome regulatory subunit 6A / Proteasome subunit beta type-1 / Proteasome subunit alpha type-1 / Proteasome subunit alpha type-2 ...26S proteasome regulatory subunit 10B / 26S proteasome non-ATPase regulatory subunit 11 / 26S proteasome non-ATPase regulatory subunit 12 / Ubiquitin C-terminal hydrolase PSMD14 / Proteasome subunit alpha type-7 / 26S proteasome non-ATPase regulatory subunit 3 / 26S proteasome regulatory subunit 6A / Proteasome subunit beta type-1 / Proteasome subunit alpha type-1 / Proteasome subunit alpha type-2 / Proteasome subunit alpha type-3 / Proteasome subunit alpha type-4 / Proteasome subunit alpha type-5 / Proteasome subunit beta type-4 / Proteasome subunit beta type-6 / Proteasome subunit beta type-5 / 26S proteasome regulatory subunit 7 / 26S proteasome regulatory subunit 6B / 26S proteasome non-ATPase regulatory subunit 8 / Proteasome subunit beta type-3 / Proteasome subunit beta type-2 / 26S proteasome non-ATPase regulatory subunit 7 / 26S proteasome non-ATPase regulatory subunit 4 / 26S proteasome complex subunit SEM1 / Proteasome subunit alpha type-6 / 26S proteasome regulatory subunit 4 / 26S proteasome regulatory subunit 8 / 26S proteasome regulatory subunit 10B / 26S proteasome non-ATPase regulatory subunit 2 / 26S proteasome non-ATPase regulatory subunit 6 / Proteasome subunit beta type-7 / 26S proteasome non-ATPase regulatory subunit 1 / 26S proteasome non-ATPase regulatory subunit 13
Similarity search - Component
Biological speciesHomo sapiens (human) / Saccharomyces cerevisiae RM11-1a (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsMao YD
Funding support China, United States, 3 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)11774012 China
National Natural Science Foundation of China (NSFC)91530321 China
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)GM43601 United States
CitationJournal: Nature / Year: 2019
Title: Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome.
Authors: Yuanchen Dong / Shuwen Zhang / Zhaolong Wu / Xuemei Li / Wei Li Wang / Yanan Zhu / Svetla Stoilova-McPhie / Ying Lu / Daniel Finley / Youdong Mao /
Abstract: The proteasome is an ATP-dependent, 2.5-megadalton molecular machine that is responsible for selective protein degradation in eukaryotic cells. Here we present cryo-electron microscopy structures of ...The proteasome is an ATP-dependent, 2.5-megadalton molecular machine that is responsible for selective protein degradation in eukaryotic cells. Here we present cryo-electron microscopy structures of the substrate-engaged human proteasome in seven conformational states at 2.8-3.6 Å resolution, captured during breakdown of a polyubiquitylated protein. These structures illuminate a spatiotemporal continuum of dynamic substrate-proteasome interactions from ubiquitin recognition to substrate translocation, during which ATP hydrolysis sequentially navigates through all six ATPases. There are three principal modes of coordinated hydrolysis, featuring hydrolytic events in two oppositely positioned ATPases, in two adjacent ATPases and in one ATPase at a time. These hydrolytic modes regulate deubiquitylation, initiation of translocation and processive unfolding of substrates, respectively. Hydrolysis of ATP powers a hinge-like motion in each ATPase that regulates its substrate interaction. Synchronization of ATP binding, ADP release and ATP hydrolysis in three adjacent ATPases drives rigid-body rotations of substrate-bound ATPases that are propagated unidirectionally in the ATPase ring and unfold the substrate.
History
DepositionOct 16, 2018-
Header (metadata) releaseNov 21, 2018-
Map releaseNov 21, 2018-
UpdateOct 23, 2024-
Current statusOct 23, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.006
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.006
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6msk
  • Surface level: 0.006
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_9222.png

Downloads & links

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Map

FileDownload / File: emd_9222.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationThe complete map of state ED2 of substrate-engaged human proteasome, low pass-filtered to 3 Angstrom without amplitude correction
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.69 Å/pix.
x 600 pix.
= 411. Å		0.69 Å/pix.
x 600 pix.
= 411. Å		0.69 Å/pix.
x 600 pix.
= 411. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.685 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.006 / Movie #1: 0.006
Minimum - Maximum-0.004926728 - 0.018093748
Average (Standard dev.)0.000040895527 (±0.0009472441)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions600600600
Spacing600600600
CellA=B=C: 411.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.6850.6850.685
M x/y/z600600600
origin x/y/z0.0000.0000.000
length x/y/z411.000411.000411.000
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ240240240
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS600600600
D min/max/mean-0.0050.0180.000

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Supplemental data

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Additional map: The complete map of state ED2 of substrate-engaged...

Fileemd_9222_additional_1.map
AnnotationThe complete map of state ED2 of substrate-engaged human proteasome, low pass-filtered to 3 Angstrom with amplitude correction with a B-factor of -40
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Unfiltered, uncorrected raw ED2 map of complete holoenzyme

Fileemd_9222_additional_2.map
AnnotationUnfiltered, uncorrected raw ED2 map of complete holoenzyme
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Proteasome

EntireName: Proteasome
Components
  • Complex: Proteasome
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 1
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 3
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 12
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 11
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 6
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 7
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 13
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 4
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 8
    • Protein or peptide: 26S proteasome complex subunit SEM1
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 2
    • Protein or peptide: 26S proteasome regulatory subunit 7
    • Protein or peptide: 26S proteasome regulatory subunit 6B
    • Protein or peptide: 26S proteasome regulatory subunit 10B
    • Protein or peptide: 26S proteasome regulatory subunit 6A
    • Protein or peptide: substrate
    • Protein or peptide: Proteasome subunit alpha type-6
    • Protein or peptide: Proteasome subunit alpha type-2
    • Protein or peptide: Proteasome subunit alpha type-4
    • Protein or peptide: Proteasome subunit alpha type-7
    • Protein or peptide: Proteasome subunit alpha type-5
    • Protein or peptide: Proteasome subunit alpha type-1
    • Protein or peptide: Proteasome subunit alpha type-3
    • Protein or peptide: Proteasome subunit beta type-6
    • Protein or peptide: Proteasome subunit beta type-7
    • Protein or peptide: Proteasome subunit beta type-3
    • Protein or peptide: Proteasome subunit beta type-2
    • Protein or peptide: Proteasome subunit beta type-5
    • Protein or peptide: Proteasome subunit beta type-1
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 14
  • Protein or peptide: Rpt2, NP_002793.2 (26SHA chain B)
  • Protein or peptide: 26S proteasome regulatory subunit 8
  • Protein or peptide: Proteasome subunit beta type-4
  • Ligand: ZINC ION
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION
  • Ligand: ADENOSINE-5'-DIPHOSPHATE

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Supramolecule #1: Proteasome

SupramoleculeName: Proteasome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#8, #10-#13, #16-#32
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: 26S proteasome non-ATPase regulatory subunit 1

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 105.958234 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MITSAAGIIS LLDEDEPQLK EFALHKLNAV VNDFWAEISE SVDKIEVLYE DEGFRSRQFA ALVASKVFYH LGAFEESLNY ALGAGDLFN VNDNSEYVET IIAKCIDHYT KQCVENADLP EGEKKPIDQR LEGIVNKMFQ RCLDDHKYKQ AIGIALETRR L DVFEKTIL ...String:
MITSAAGIIS LLDEDEPQLK EFALHKLNAV VNDFWAEISE SVDKIEVLYE DEGFRSRQFA ALVASKVFYH LGAFEESLNY ALGAGDLFN VNDNSEYVET IIAKCIDHYT KQCVENADLP EGEKKPIDQR LEGIVNKMFQ RCLDDHKYKQ AIGIALETRR L DVFEKTIL ESNDVPGMLA YSLKLCMSLM QNKQFRNKVL RVLVKIYMNL EKPDFINVCQ CLIFLDDPQA VSDILEKLVK ED NLLMAYQ ICFDLYESAS QQFLSSVIQN LRTVGTPIAS VPGSTNTGTV PGSEKDSDSM ETEEKTSSAF VGKTPEASPE PKD QTLKMI KILSGEMAIE LHLQFLIRNN NTDLMILKNT KDAVRNSVCH TATVIANSFM HCGTTSDQFL RDNLEWLARA TNWA KFTAT ASLGVIHKGH EKEALQLMAT YLPKDTSPGS AYQEGGGLYA LGLIHANHGG DIIDYLLNQL KNASNDIVRH GGSLG LGLA AMGTARQDVY DLLKTNLYQD DAVTGEAAGL ALGLVMLGSK NAQAIEDMVG YAQETQHEKI LRGLAVGIAL VMYGRM EEA DALIESLCRD KDPILRRSGM YTVAMAYCGS GNNKAIRRLL HVAVSDVNDD VRRAAVESLG FILFRTPEQC PSVVSLL SE SYNPHVRYGA AMALGICCAG TGNKEAINLL EPMTNDPVNY VRQGALIASA LIMIQQTEIT CPKVNQFRQL YSKVINDK H DDVMAKFGAI LAQGILDAGG HNVTISLQSR TGHTHMPSVV GVLVFTQFWF WFPLSHFLSL AYTPTCVIGL NKDLKMPKV QYKSNCKPST FAYPAPLEVP KEKEKEKVST AVLSITAKAK KKEKEKEKKE EEKMEVDEAE KKEEKEKKKE PEPNFQLLDN PARVMPAQL KVLTMPETCR YQPFKPLSIG GIIILKDTSE DIEELVEPVA AHGPKIEEEE QEPEPPEPFE YIDD

UniProtKB: 26S proteasome non-ATPase regulatory subunit 1

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Macromolecule #2: 26S proteasome non-ATPase regulatory subunit 3

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 60.935297 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: KQEGSARRRG ADKAKPPPGG GEQEPPPPPA PQDVEMKEEA ATGGGSTGEA DGKTAAAAAE HSQRELDTVT LEDIKEHVKQ LEKAVSGKE PRFVLRALRM LPSTSRRLNH YVLYKAVQGF FTSNNATRDF LLPFLEEPMD TEADLQFRPR TGKAASTPLL P EVEAYLQL ...String:
KQEGSARRRG ADKAKPPPGG GEQEPPPPPA PQDVEMKEEA ATGGGSTGEA DGKTAAAAAE HSQRELDTVT LEDIKEHVKQ LEKAVSGKE PRFVLRALRM LPSTSRRLNH YVLYKAVQGF FTSNNATRDF LLPFLEEPMD TEADLQFRPR TGKAASTPLL P EVEAYLQL LVVIFMMNSK RYKEAQKISD DLMQKISTQN RRALDLVAAK CYYYHARVYE FLDKLDVVRS FLHARLRTAT LR HDADGQA TLLNLLLRNY LHYSLYDQAE KLVSKSVFPE QANNNEWARY LYYTGRIKAI QLEYSEARRT MTNALRKAPQ HTA VGFKQT VHKLLIVVEL LLGEIPDRLQ FRQPSLKRSL MPYFLLTQAV RTGNLAKFNQ VLDQFGEKFQ ADGTYTLIIR LRHN VIKTG VRMISLSYSR ISLADIAQKL QLDSPEDAEF IVAKAIRDGV IEASINHEKG YVQSKEMIDI YSTREPQLAF HQRIS FCLD IHNMSVKAMR FPPKSYNKDL ESAEERRERE QQDLEFAKEM AEDDDDSFP

UniProtKB: 26S proteasome non-ATPase regulatory subunit 3

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Macromolecule #3: 26S proteasome non-ATPase regulatory subunit 12

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 12 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 52.979359 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MADGGSERAD GRIVKMEVDY SATVDQRLPE CAKLAKEGRL QEVIETLLSL EKQTRTASDM VSTSRILVAV VKMCYEAKEW DLLNENIML LSKRRSQLKQ AVAKMVQQCC TYVEEITDLP IKLRLIDTLR MVTEGKIYVE IERARLTKTL ATIKEQNGDV K EAASILQE ...String:
MADGGSERAD GRIVKMEVDY SATVDQRLPE CAKLAKEGRL QEVIETLLSL EKQTRTASDM VSTSRILVAV VKMCYEAKEW DLLNENIML LSKRRSQLKQ AVAKMVQQCC TYVEEITDLP IKLRLIDTLR MVTEGKIYVE IERARLTKTL ATIKEQNGDV K EAASILQE LQVETYGSME KKERVEFILE QMRLCLAVKD YIRTQIISKK INTKFFQEEN TEKLKLKYYN LMIQLDQHEG SY LSICKHY RAIYDTPCIQ AESEKWQQAL KSVVLYVILA PFDNEQSDLV HRISGDKKLE EIPKYKDLLK LFTTMELMRW STL VEDYGM ELRKGSLESP ATDVFGSTEE GEKRWKDLKN RVVEHNIRIM AKYYTRITMK RMAQLLDLSV DESEAFLSNL VVNK TIFAK VDRLAGIINF QRPKDPNNLL NDWSQKLNSL MSLVNKTTHL IAKEEMIHNL Q

UniProtKB: 26S proteasome non-ATPase regulatory subunit 12

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Macromolecule #4: 26S proteasome non-ATPase regulatory subunit 11

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 11 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 47.526688 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAAAAVVEFQ RAQSLLSTDR EASIDILHSI VKRDIQENDE EAVQVKEQSI LELGSLLAKT GQAAELGGLL KYVRPFLNSI SKAKAARLV RSLLDLFLDM EAATGQEVEL CLECIEWAKS EKRTFLRQAL EARLVSLYFD TKRYQEALHL GSQLLRELKK M DDKALLVE ...String:
MAAAAVVEFQ RAQSLLSTDR EASIDILHSI VKRDIQENDE EAVQVKEQSI LELGSLLAKT GQAAELGGLL KYVRPFLNSI SKAKAARLV RSLLDLFLDM EAATGQEVEL CLECIEWAKS EKRTFLRQAL EARLVSLYFD TKRYQEALHL GSQLLRELKK M DDKALLVE VQLLESKTYH ALSNLPKARA ALTSARTTAN AIYCPPKLQA TLDMQSGIIH AAEEKDWKTA YSYFYEAFEG YD SIDSPKA ITSLKYMLLC KIMLNTPEDV QALVSGKLAL RYAGRQTEAL KCVAQASKNR SLADFEKALT DYRAELRDDP IIS THLAKL YDNLLEQNLI RVIEPFSRVQ IEHISSLIKL SKADVERKLS QMILDKKFHG ILDQGEGVLI IFDEPPVDKT YEAA LETIQ NMSKVVDSLY NKAKKLT

UniProtKB: 26S proteasome non-ATPase regulatory subunit 11

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Macromolecule #5: 26S proteasome non-ATPase regulatory subunit 6

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.592285 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MPLENLEEEG LPKNPDLRIA QLRFLLSLPE HRGDAAVRDE LMAAVRDNNM APYYEALCKS LDWQIDVDLL NKMKKANEDE LKRLDEELE DAEKNLGESE IRDAMMAKAE YLCRIGDKEG ALTAFRKTYD KTVALGHRLD IVFYLLRIGL FYMDNDLITR N TEKAKSLI ...String:
MPLENLEEEG LPKNPDLRIA QLRFLLSLPE HRGDAAVRDE LMAAVRDNNM APYYEALCKS LDWQIDVDLL NKMKKANEDE LKRLDEELE DAEKNLGESE IRDAMMAKAE YLCRIGDKEG ALTAFRKTYD KTVALGHRLD IVFYLLRIGL FYMDNDLITR N TEKAKSLI EEGGDWDRRN RLKVYQGLYC VAIRDFKQAA ELFLDTVSTF TSYELMDYKT FVTYTVYVSM IALERPDLRE KV IKGAEIL EVLHSLPAVR QYLFSLYECR YSVFFQSLAV VEQEMKKDWL FAPHYRYYVR EMRIHAYSQL LESYRSLTLG YMA EAFGVG VEFIDQELSR FIAAGRLHCK IDKVNEIVET NRPDSKNWQY QETIKKGDLL LNRVQKLSRV INM

UniProtKB: 26S proteasome non-ATPase regulatory subunit 6

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Macromolecule #6: 26S proteasome non-ATPase regulatory subunit 7

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 7 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 37.086441 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MPELAVQKVV VHPLVLLSVV DHFNRIGKVG NQKRVVGVLL GSWQKKVLDV SNSFAVPFDE DDKDDSVWFL DHDYLENMYG MFKKVNARE RIVGWYHTGP KLHKNDIAIN ELMKRYCPNS VLVIIDVKPK DLGLPTEAYI SVEEVHDDGT PTSKTFEHVT S EIGAEEAE ...String:
MPELAVQKVV VHPLVLLSVV DHFNRIGKVG NQKRVVGVLL GSWQKKVLDV SNSFAVPFDE DDKDDSVWFL DHDYLENMYG MFKKVNARE RIVGWYHTGP KLHKNDIAIN ELMKRYCPNS VLVIIDVKPK DLGLPTEAYI SVEEVHDDGT PTSKTFEHVT S EIGAEEAE EVGVEHLLRD IKDTTVGTLS QRITNQVHGL KGLNSKLLDI RSYLEKVATG KLPINHQIIY QLQDVFNLLP DV SLQEFVK AFYLKTNDQM VVVYLASLIR SVVALHNLIN NKIANRDAEK KEGQEKEESK KDRKEDKEKD KDKEKSDVKK EEK KEKK

UniProtKB: 26S proteasome non-ATPase regulatory subunit 7

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Macromolecule #7: 26S proteasome non-ATPase regulatory subunit 13

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 13 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 42.995359 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MKDVPGFLQQ SQNSGPGQPA VWHRLEELYT KKLWHQLTLQ VLDFVQDPCF AQGDGLIKLY ENFISEFEHR VNPLSLVEII LHVVRQMTD PNVALTFLEK TREKVKSSDE AVILCKTAIG ALKLNIGDLQ VTKETIEDVE EMLNNLPGVT SVHSRFYDLS S KYYQTIGN ...String:
MKDVPGFLQQ SQNSGPGQPA VWHRLEELYT KKLWHQLTLQ VLDFVQDPCF AQGDGLIKLY ENFISEFEHR VNPLSLVEII LHVVRQMTD PNVALTFLEK TREKVKSSDE AVILCKTAIG ALKLNIGDLQ VTKETIEDVE EMLNNLPGVT SVHSRFYDLS S KYYQTIGN HASYYKDALR FLGCVDIKDL PVSEQQERAF TLGLAGLLGE GVFNFGELLM HPVLESLRNT DRQWLIDTLY AF NSGNVER FQTLKTAWGQ QPDLAANEAQ LLRKIQLLCL MEMTFTRPAN HRQLTFEEIA KSAKITVNEV ELLVMKALSV GLV KGSIDE VDKRVHMTWV QPRVLDLQQI KGMKDRLEFW CTDVKSMEML VEHQAHDILT

UniProtKB: 26S proteasome non-ATPase regulatory subunit 13

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Macromolecule #8: 26S proteasome non-ATPase regulatory subunit 4

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 4 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 40.78159 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MVLESTMVCV DNSEYMRNGD FLPTRLQAQQ DAVNIVCHSK TRSNPENNVG LITLANDCEV LTTLTPDTGR ILSKLHTVQP KGKITFCTG IRVAHLALKH RQGKNHKMRI IAFVGSPVED NEKDLVKLAK RLKKEKVNVD IINFGEEEVN TEKLTAFVNT L NGKDGTGS ...String:
MVLESTMVCV DNSEYMRNGD FLPTRLQAQQ DAVNIVCHSK TRSNPENNVG LITLANDCEV LTTLTPDTGR ILSKLHTVQP KGKITFCTG IRVAHLALKH RQGKNHKMRI IAFVGSPVED NEKDLVKLAK RLKKEKVNVD IINFGEEEVN TEKLTAFVNT L NGKDGTGS HLVTVPPGPS LADALISSPI LAGEGGAMLG LGASDFEFGV DPSADPELAL ALRVSMEEQR QRQEEEARRA AA ASAAEAG IATTGTEDSD DALLKMTISQ QEFGRTGLPD LSSMTEEEQI AYAMQMSLQG AEFGQAESAD IDASSAMDTS EPA KEEDDY DVMQDPEFLQ SVLENLPGVD PNNEAIRNAM GSLASQATKD GKKDKKEEDK K

UniProtKB: 26S proteasome non-ATPase regulatory subunit 4

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Macromolecule #9: 26S proteasome non-ATPase regulatory subunit 14

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 14 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
EC number: Hydrolases; Acting on peptide bonds (peptidases); Omega peptidases
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 34.488824 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: DRLLRLGGGM PGLGQGPPTD APAVDTAEQV YISSLALLKM LKHGRAGVPM EVMGLMLGEF VDDYTVRVID VFAMPQSGTG VSVEAVDPV FQAKMLDMLK QTGRPEMVVG WYHSHPGFGC WLSGVDINTQ QSFEALSERA VAVVVDPIQS VKGKVVIDAF R LINANMMV ...String:
DRLLRLGGGM PGLGQGPPTD APAVDTAEQV YISSLALLKM LKHGRAGVPM EVMGLMLGEF VDDYTVRVID VFAMPQSGTG VSVEAVDPV FQAKMLDMLK QTGRPEMVVG WYHSHPGFGC WLSGVDINTQ QSFEALSERA VAVVVDPIQS VKGKVVIDAF R LINANMMV LGHEPRQTTS NLGHLNKPSI QALIHGLNRH YYSITINYRK NELEQKMLLN LHKKSWMEGL TLQDYSEHCK HN ESVVKEM LELAKNYNKA VEEEDKMTPE QLAIKNVGKQ DPKRHLEEHV DVLMTSNIVQ CLAAMLDTVV FK

UniProtKB: Ubiquitin C-terminal hydrolase PSMD14

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Macromolecule #10: 26S proteasome non-ATPase regulatory subunit 8

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 8 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 39.536676 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: FIKGRAPRAP PRERRRATRG GLRQVVAPPR ALGSTSRPHF RRASVCRRRC RKSGGLLAAS RKMAAAAVNG AAGFSSSGPA ATSGAVLQA ATGMYEQLKG EWNRKSPNLS KCGEELGRLK LVLLELNFLP TTGTKLTKQQ LILARDILEI GAQWSILRKD I PSFERYMA ...String:
FIKGRAPRAP PRERRRATRG GLRQVVAPPR ALGSTSRPHF RRASVCRRRC RKSGGLLAAS RKMAAAAVNG AAGFSSSGPA ATSGAVLQA ATGMYEQLKG EWNRKSPNLS KCGEELGRLK LVLLELNFLP TTGTKLTKQQ LILARDILEI GAQWSILRKD I PSFERYMA QLKCYYFDYK EQLPESAYMH QLLGLNLLFL LSQNRVAEFH TELERLPAKD IQTNVYIKHP VSLEQYLMEG SY NKVFLAK GNIPAESYTF FIDILLDTIR DEIAGCIEKA YEKILFTEAT RILFFNTPKK MTDYAKKRGW VLGPNNYYSF ASQ QQKPED TTIPSTELAK QVIEYARQLE MIV

UniProtKB: 26S proteasome non-ATPase regulatory subunit 8

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Macromolecule #11: 26S proteasome complex subunit SEM1

MacromoleculeName: 26S proteasome complex subunit SEM1 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 8.284611 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MSEKKQPVDL GLLEEDDEFE EFPAEDWAGL DEDEDAHVWE DNWDDDNVED DFSNQLRAEL EKHGYKMETS

UniProtKB: 26S proteasome complex subunit SEM1

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Macromolecule #12: 26S proteasome non-ATPase regulatory subunit 2

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 2 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 98.425414 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MEEGGRDKAP VQPQQSPAAA PGGTDEKPSG KERRDAGDKD KEQELSEEDK QLQDELEMLV ERLGEKDTSL YRPALEELRR QIRSSTTSM TSVPKPLKFL RPHYGKLKEI YENMAPGENK RFAADIISVL AMTMSGEREC LKYRLVGSQE ELASWGHEYV R HLAGEVAK ...String:
MEEGGRDKAP VQPQQSPAAA PGGTDEKPSG KERRDAGDKD KEQELSEEDK QLQDELEMLV ERLGEKDTSL YRPALEELRR QIRSSTTSM TSVPKPLKFL RPHYGKLKEI YENMAPGENK RFAADIISVL AMTMSGEREC LKYRLVGSQE ELASWGHEYV R HLAGEVAK EWQELDDAEK VQREPLLTLV KEIVPYNMAH NAEHEACDLL MEIEQVDMLE KDIDENAYAK VCLYLTSCVN YV PEPENSA LLRCALGVFR KFSRFPEALR LALMLNDMEL VEDIFTSCKD VVVQKQMAFM LGRHGVFLEL SEDVEEYEDL TEI MSNVQL NSNFLALARE LDIMEPKVPD DIYKTHLENN RFGGSGSQVD SARMNLASSF VNGFVNAAFG QDKLLTDDGN KWLY KNKDH GMLSAAASLG MILLWDVDGG LTQIDKYLYS SEDYIKSGAL LACGIVNSGV RNECDPALAL LSDYVLHNSN TMRLG SIFG LGLAYAGSNR EDVLTLLLPV MGDSKSSMEV AGVTALACGM IAVGSCNGDV TSTILQTIME KSETELKDTY ARWLPL GLG LNHLGKGEAI EAILAALEVV SEPFRSFANT LVDVCAYAGS GNVLKVQQLL HICSEHFDSK EKEEDKDKKE KKDKDKK EA PADMGAHQGV AVLGIALIAM GEEIGAEMAL RTFGHLLRYG EPTLRRAVPL ALALISVSNP RLNILDTLSK FSHDADPE V SYNSIFAMGM VGSGTNNARL AAMLRQLAQY HAKDPNNLFM VRLAQGLTHL GKGTLTLCPY HSDRQLMSQV AVAGLLTVL VSFLDVRNII LGKSHYVLYG LVAAMQPRML VTFDEELRPL PVSVRVGQAV DVVGQAGKPK TITGFQTHTT PVLLAHGERA ELATEEFLP VTP

UniProtKB: 26S proteasome non-ATPase regulatory subunit 2

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Macromolecule #13: 26S proteasome regulatory subunit 7

MacromoleculeName: 26S proteasome regulatory subunit 7 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 48.700805 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MPDYLGADQR KTKEDEKDDK PIRALDEGDI ALLKTYGQST YSRQIKQVED DIQQLLKKIN ELTGIKESDT GLAPPALWDL AADKQTLQS EQPLQVARCT KIINADSEDP KYIINVKQFA KFVVDLSDQV APTDIEEGMR VGVDRNKYQI HIPLPPKIDP T VTMMQVEE ...String:
MPDYLGADQR KTKEDEKDDK PIRALDEGDI ALLKTYGQST YSRQIKQVED DIQQLLKKIN ELTGIKESDT GLAPPALWDL AADKQTLQS EQPLQVARCT KIINADSEDP KYIINVKQFA KFVVDLSDQV APTDIEEGMR VGVDRNKYQI HIPLPPKIDP T VTMMQVEE KPDVTYSDVG GCKEQIEKLR EVVETPLLHP ERFVNLGIEP PKGVLLFGPP GTGKTLCARA VANRTDACFI RV IGSELVQ KYVGEGARMV RELFEMARTK KACLIFFDEI DAIGGARFDD GAGGDNEVQR TMLELINQLD GFDPRGNIKV LMA TNRPDT LDPALMRPGR LDRKIEFSLP DLEGRTHIFK IHARSMSVER DIRFELLARL CPNSTGAEIR SVCTEAGMFA IRAR RKIAT EKDFLEAVNK VIKSYAKFSA TPRYMTYN

UniProtKB: 26S proteasome regulatory subunit 7

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Macromolecule #14: Rpt2, NP_002793.2 (26SHA chain B)

MacromoleculeName: Rpt2, NP_002793.2 (26SHA chain B) / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 49.260504 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MGQSQSGGHG PGGGKKDDKD KKKKYEPPVP TRVGKKKKKT KGPDAASKLP LVTPHTQCRL KLLKLERIKD YLLMEEEFIR NQEQMKPLE EKQEEERSKV DDLRGTPMSV GTLEEIIDDN HAIVSTSVGS EHYVSILSFV DKDLLEPGCS VLLNHKVHAV I GVLMDDTD ...String:
MGQSQSGGHG PGGGKKDDKD KKKKYEPPVP TRVGKKKKKT KGPDAASKLP LVTPHTQCRL KLLKLERIKD YLLMEEEFIR NQEQMKPLE EKQEEERSKV DDLRGTPMSV GTLEEIIDDN HAIVSTSVGS EHYVSILSFV DKDLLEPGCS VLLNHKVHAV I GVLMDDTD PLVTVMKVEK APQETYADIG GLDNQIQEIK ESVELPLTHP EYYEEMGIKP PKGVILYGPP GTGKTLLAKA VA NQTSATF LRVVGSELIQ KYLGDGPKLV RELFRVAEEH APSIVFIDEI DAIGTKRYDS NSGGEREIQR TMLELLNQLD GFD SRGDVK VIMATNRIET LDPALIRPGR IDRKIEFPLP DEKTKKRIFQ IHTSRMTLAD DVTLDDLIMA KDDLSGADIK AICT EAGLM ALRERRMKVT NEDFKKSKEN VLYKKQEGTP EGLYL

UniProtKB: 26S proteasome regulatory subunit 4

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Macromolecule #15: 26S proteasome regulatory subunit 8

MacromoleculeName: 26S proteasome regulatory subunit 8 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 44.852121 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MELEEGKAGS GLRQYYLSKI EELQLIVNDK SQNLRRLQAQ RNELNAKVRL LREELQLLQE QGSYVGEVVR AMDKKKVLVK VHPEGKFVV DVDKNIDIND VTPNCRVALR NDSYTLHKIL PNKVDPLVSL MMVEKVPDST YEMIGGLDKQ IKEIKEVIEL P VKHPELFE ...String:
MELEEGKAGS GLRQYYLSKI EELQLIVNDK SQNLRRLQAQ RNELNAKVRL LREELQLLQE QGSYVGEVVR AMDKKKVLVK VHPEGKFVV DVDKNIDIND VTPNCRVALR NDSYTLHKIL PNKVDPLVSL MMVEKVPDST YEMIGGLDKQ IKEIKEVIEL P VKHPELFE ALGIAQPKGV LLYGPPGTGK TLLARAVAHH TDCTFIRVSG SELVQKFIGE GARMVRELFV MAREHAPSII FM DEIDSIG SSRLEGGSGG DSEVQRTMLE LLNQLDGFEA TKNIKVIMAT NRIDILDSAL LRPGRIDRKI EFPPPNEEAR LDI LKIHSR KMNLTRGINL RKIAELMPGA SGAEVKGVCT EAGMYALRER RVHVTQEDFE MAVAKVMQKD SEKNMSIKKL WK

UniProtKB: 26S proteasome regulatory subunit 8

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Macromolecule #16: 26S proteasome regulatory subunit 6B

MacromoleculeName: 26S proteasome regulatory subunit 6B / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 47.426141 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MEEIGILVEK AQDEIPALSV SRPQTGLSFL GPEPEDLEDL YSRYKKLQQE LEFLEVQEEY IKDEQKNLKK EFLHAQEEVK RIQSIPLVI GQFLEAVDQN TAIVGSTTGS NYYVRILSTI DRELLKPNAS VALHKHSNAL VDVLPPEADS SIMMLTSDQK P DVMYADIG ...String:
MEEIGILVEK AQDEIPALSV SRPQTGLSFL GPEPEDLEDL YSRYKKLQQE LEFLEVQEEY IKDEQKNLKK EFLHAQEEVK RIQSIPLVI GQFLEAVDQN TAIVGSTTGS NYYVRILSTI DRELLKPNAS VALHKHSNAL VDVLPPEADS SIMMLTSDQK P DVMYADIG GMDIQKQEVR EAVELPLTHF ELYKQIGIDP PRGVLMYGPP GCGKTMLAKA VAHHTTAAFI RVVGSEFVQK YL GEGPRMV RDVFRLAKEN APAIIFIDEI DAIATKRFDA QTGADREVQR ILLELLNQMD GFDQNVNVKV IMATNRADTL DPA LLRPGR LDRKIEFPLP DRRQKRLIFS TITSKMNLSE EVDLEDYVAR PDKISGADIN SICQESGMLA VRENRYIVLA KDFE KAYKT VIKKDEQEHE FYK

UniProtKB: 26S proteasome regulatory subunit 6B

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Macromolecule #17: 26S proteasome regulatory subunit 10B

MacromoleculeName: 26S proteasome regulatory subunit 10B / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.867027 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MAIPGIPYER RLLIMADPRD KALQDYRKKL LEHKEIDGRL KELREQLKEL TKQYEKSEND LKALQSVGQI VGEVLKQLTE EKFIVKATN GPRYVVGCRR QLDKSKLKPG TRVALDMTTL TIMRYLPREV DPLVYNMSHE DPGNVSYSEI GGLSEQIREL R EVIELPLT ...String:
MAIPGIPYER RLLIMADPRD KALQDYRKKL LEHKEIDGRL KELREQLKEL TKQYEKSEND LKALQSVGQI VGEVLKQLTE EKFIVKATN GPRYVVGCRR QLDKSKLKPG TRVALDMTTL TIMRYLPREV DPLVYNMSHE DPGNVSYSEI GGLSEQIREL R EVIELPLT NPELFQRVGI IPPKGCLLYG PPGTGKTLLA RAVASQLDCN FLKVVSSSIV DKYIGESARL IREMFNYARD HQ PCIIFMD EIDAIGGRRF SEGTSADREI QRTLMELLNQ MDGFDTLHRV KMIMATNRPD TLDPALLRPG RLDRKIHIDL PNE QARLDI LKIHAGPITK HGEIDYEAIV KLSDGFNGAD LRNVCTEAGM FAIRADHDFV VQEDFMKAVR KVADSKKLES KLDY KPV

UniProtKB: 26S proteasome regulatory subunit 10B

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Macromolecule #18: 26S proteasome regulatory subunit 6A

MacromoleculeName: 26S proteasome regulatory subunit 6A / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 49.266457 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MNLLPNIESP VTRQEKMATV WDEAEQDGIG EEVLKMSTEE IIQRTRLLDS EIKIMKSEVL RVTHELQAMK DKIKENSEKI KVNKTLPYL VSNVIELLDV DPNDQEEDGA NIDLDSQRKG KCAVIKTSTR QTYFLPVIGL VDAEKLKPGD LVGVNKDSYL I LETLPTEY ...String:
MNLLPNIESP VTRQEKMATV WDEAEQDGIG EEVLKMSTEE IIQRTRLLDS EIKIMKSEVL RVTHELQAMK DKIKENSEKI KVNKTLPYL VSNVIELLDV DPNDQEEDGA NIDLDSQRKG KCAVIKTSTR QTYFLPVIGL VDAEKLKPGD LVGVNKDSYL I LETLPTEY DSRVKAMEVD ERPTEQYSDI GGLDKQIQEL VEAIVLPMNH KEKFENLGIQ PPKGVLMYGP PGTGKTLLAR AC AAQTKAT FLKLAGPQLV QMFIGDGAKL VRDAFALAKE KAPSIIFIDE LDAIGTKRFD SEKAGDREVQ RTMLELLNQL DGF QPNTQV KVIAATNRVD ILDPALLRSG RLDRKIEFPM PNEEARARIM QIHSRKMNVS PDVNYEELAR CTDDFNGAQC KAVC VEAGM IALRRGATEL THEDYMEGIL EVQAKKKANL QYYA

UniProtKB: 26S proteasome regulatory subunit 6A

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Macromolecule #19: substrate

MacromoleculeName: substrate / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae RM11-1a (yeast)
Molecular weightTheoretical: 3.08179 KDa
Recombinant expressionOrganism: Escherichia coli K-12 (bacteria)
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

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Macromolecule #20: Proteasome subunit alpha type-6

MacromoleculeName: Proteasome subunit alpha type-6 / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 27.301262 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SRGSSAGFDR HITIFSPEGR LYQVEYAFKA INQGGLTSVA VRGKDCAVIV TQKKVPDKLL DSSTVTHLFK ITENIGCVMT GMTADSRSQ VQRARYEAAN WKYKYGYEIP VDMLCKRIAD ISQVYTQNAE MRPLGCCMIL IGIDEEQGPQ VYKCDPAGYY C GFKATAAG ...String:
SRGSSAGFDR HITIFSPEGR LYQVEYAFKA INQGGLTSVA VRGKDCAVIV TQKKVPDKLL DSSTVTHLFK ITENIGCVMT GMTADSRSQ VQRARYEAAN WKYKYGYEIP VDMLCKRIAD ISQVYTQNAE MRPLGCCMIL IGIDEEQGPQ VYKCDPAGYY C GFKATAAG VKQTESTSFL EKKVKKKFDW TFEQTVETAI TCLSTVLSID FKPSEIEVGV VTVENPKFRI LTEAEIDAHL VA LAERD

UniProtKB: Proteasome subunit alpha type-6

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Macromolecule #21: Proteasome subunit alpha type-2

MacromoleculeName: Proteasome subunit alpha type-2 / type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.796338 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: AERGYSFSLT TFSPSGKLVQ IEYALAAVAG GAPSVGIKAA NGVVLATEKK QKSILYDERS VHKVEPITKH IGLVYSGMGP DYRVLVHRA RKLAQQYYLV YQEPIPTAQL VQRVASVMQE YTQSGGVRPF GVSLLICGWN EGRPYLFQSD PSGAYFAWKA T AMGKNYVN ...String:
AERGYSFSLT TFSPSGKLVQ IEYALAAVAG GAPSVGIKAA NGVVLATEKK QKSILYDERS VHKVEPITKH IGLVYSGMGP DYRVLVHRA RKLAQQYYLV YQEPIPTAQL VQRVASVMQE YTQSGGVRPF GVSLLICGWN EGRPYLFQSD PSGAYFAWKA T AMGKNYVN GKTFLEKRYN EDLELEDAIH TAILTLKESF EGQMTEDNIE VGICNEAGFR RLTPTEVKDY LAAIA

UniProtKB: Proteasome subunit alpha type-2

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Macromolecule #22: Proteasome subunit alpha type-4

MacromoleculeName: Proteasome subunit alpha type-4 / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.394648 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SRRYDSRTTI FSPEGRLYQV EYAMEAIGHA GTCLGILAND GVLLAAERRN IHKLLDEVFF SEKIYKLNED MACSVAGITS DANVLTNEL RLIAQRYLLQ YQEPIPCEQL VTALCDIKQA YTQFGGKRPF GVSLLYIGWD KHYGFQLYQS DPSGNYGGWK A TCIGNNSA ...String:
SRRYDSRTTI FSPEGRLYQV EYAMEAIGHA GTCLGILAND GVLLAAERRN IHKLLDEVFF SEKIYKLNED MACSVAGITS DANVLTNEL RLIAQRYLLQ YQEPIPCEQL VTALCDIKQA YTQFGGKRPF GVSLLYIGWD KHYGFQLYQS DPSGNYGGWK A TCIGNNSA AAVSMLKQDY KEGEMTLKSA LALAIKVLNK TMDVSKLSAE KVEIATLTRE NGKTVIRVLK QKEVEQLIKK HE EEEAKAE REKKEKEQKE KDK

UniProtKB: Proteasome subunit alpha type-4

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Macromolecule #23: Proteasome subunit alpha type-7

MacromoleculeName: Proteasome subunit alpha type-7 / type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 27.798695 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SYDRAITVFS PDGHLFQVEY AQEAVKKGST AVGVRGRDIV VLGVEKKSVA KLQDERTVRK ICALDDNVCM AFAGLTADAR IVINRARVE CQSHRLTVED PVTVEYITRY IASLKQRYTQ SNGRRPFGIS ALIVGFDFDG TPRLYQTDPS GTYHAWKANA I GRGAKSVR ...String:
SYDRAITVFS PDGHLFQVEY AQEAVKKGST AVGVRGRDIV VLGVEKKSVA KLQDERTVRK ICALDDNVCM AFAGLTADAR IVINRARVE CQSHRLTVED PVTVEYITRY IASLKQRYTQ SNGRRPFGIS ALIVGFDFDG TPRLYQTDPS GTYHAWKANA I GRGAKSVR EFLEKNYTDE AIETDDLTIK LVIKALLEVV QSGGKNIELA VMRRDQSLKI LNPEEIEKYV AEIEKEKEEN EK KKQKKAS

UniProtKB: Proteasome subunit alpha type-7

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Macromolecule #24: Proteasome subunit alpha type-5

MacromoleculeName: Proteasome subunit alpha type-5 / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 26.304779 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: FLTRSEYDRG VNTFSPEGRL FQVEYAIEAI KLGSTAIGIQ TSEGVCLAVE KRITSPLMEP SSIEKIVEID AHIGCAMSGL IADAKTLID KARVETQNHW FTYNETMTVE SVTQAVSNLA LQFGEEDADP GAMSRPFGVA LLFGGVDEKG PQLFHMDPSG T FVQCDARA ...String:
FLTRSEYDRG VNTFSPEGRL FQVEYAIEAI KLGSTAIGIQ TSEGVCLAVE KRITSPLMEP SSIEKIVEID AHIGCAMSGL IADAKTLID KARVETQNHW FTYNETMTVE SVTQAVSNLA LQFGEEDADP GAMSRPFGVA LLFGGVDEKG PQLFHMDPSG T FVQCDARA IGSASEGAQS SLQEVYHKSM TLKEAIKSSL IILKQVMEEK LNATNIELAT VQPGQNFHMF TKEELEEVIK DI

UniProtKB: Proteasome subunit alpha type-5

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Macromolecule #25: Proteasome subunit alpha type-1

MacromoleculeName: Proteasome subunit alpha type-1 / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 30.150277 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: QLSKVKFRNQ YDNDVTVWSP QGRIHQIEYA MEAVKQGSAT VGLKSKTHAV LVALKRAQSE LAAHQKKILH VDNHIGISIA GLTADARLL CNFMRQECLD SRFVFDRPLP VSRLVSLIGS KTQIPTQRYG RRPYGVGLLI AGYDDMGPHI FQTCPSANYF D CRAMSIGA ...String:
QLSKVKFRNQ YDNDVTVWSP QGRIHQIEYA MEAVKQGSAT VGLKSKTHAV LVALKRAQSE LAAHQKKILH VDNHIGISIA GLTADARLL CNFMRQECLD SRFVFDRPLP VSRLVSLIGS KTQIPTQRYG RRPYGVGLLI AGYDDMGPHI FQTCPSANYF D CRAMSIGA RSQSARTYLE RHMSEFMECN LNELVKHGLR ALRETLPAEQ DLTTKNVSIG IVGKDLEFTI YDDDDVSPFL EG LEERPQR KAQPAQPADE PAEKADEPME H

UniProtKB: Proteasome subunit alpha type-1

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Macromolecule #26: Proteasome subunit alpha type-3

MacromoleculeName: Proteasome subunit alpha type-3 / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 28.338057 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SSIGTGYDLS ASTFSPDGRV FQVEYAMKAV ENSSTAIGIR CKDGVVFGVE KLVLSKLYEE GSNKRLFNVD RHVGMAVAGL LADARSLAD IAREEASNFR SNFGYNIPLK HLADRVAMYV HAYTLYSAVR PFGCSFMLGS YSVNDGAQLY MIDPSGVSYG Y WGCAIGKA ...String:
SSIGTGYDLS ASTFSPDGRV FQVEYAMKAV ENSSTAIGIR CKDGVVFGVE KLVLSKLYEE GSNKRLFNVD RHVGMAVAGL LADARSLAD IAREEASNFR SNFGYNIPLK HLADRVAMYV HAYTLYSAVR PFGCSFMLGS YSVNDGAQLY MIDPSGVSYG Y WGCAIGKA RQAAKTEIEK LQMKEMTCRD IVKEVAKIIY IVHDEVKDKA FELELSWVGE LTNGRHEIVP KDIREEAEKY AK ESLKEED ESDDDNM

UniProtKB: Proteasome subunit alpha type-3

+
Macromolecule #27: Proteasome subunit beta type-6

MacromoleculeName: Proteasome subunit beta type-6 / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.246455 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: AATLLAARGA GPAPAWGPEA FTPDWESREV STGTTIMAVQ FDGGVVLGAD SRTTTGSYIA NRVTDKLTPI HDRIFCCRSG SAADTQAVA DAVTYQLGFH SIELNEPPLV HTAASLFKEM CYRYREDLMA GIIIAGWDPQ EGGQVYSVPM GGMMVRQSFA I GGSGSSYI ...String:
AATLLAARGA GPAPAWGPEA FTPDWESREV STGTTIMAVQ FDGGVVLGAD SRTTTGSYIA NRVTDKLTPI HDRIFCCRSG SAADTQAVA DAVTYQLGFH SIELNEPPLV HTAASLFKEM CYRYREDLMA GIIIAGWDPQ EGGQVYSVPM GGMMVRQSFA I GGSGSSYI YGYVDATYRE GMTKEECLQF TANALALAME RDGSSGGVIR LAAIAESGVE RQVLLGDQIP KFAVATLPPA

UniProtKB: Proteasome subunit beta type-6

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Macromolecule #28: Proteasome subunit beta type-7

MacromoleculeName: Proteasome subunit beta type-7 / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.869223 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: AAVSVYAPPV GGFSFDNCRR NAVLEADFAK RGYKLPKVRK TGTTIAGVVY KDGIVLGADT RATEGMVVAD KNCSKIHFIS PNIYCCGAG TAADTDMTTQ LISSNLELHS LSTGRLPRVV TANRMLKQML FRYQGYIGAA LVLGGVDVTG PHLYSIYPHG S TDKLPYVT ...String:
AAVSVYAPPV GGFSFDNCRR NAVLEADFAK RGYKLPKVRK TGTTIAGVVY KDGIVLGADT RATEGMVVAD KNCSKIHFIS PNIYCCGAG TAADTDMTTQ LISSNLELHS LSTGRLPRVV TANRMLKQML FRYQGYIGAA LVLGGVDVTG PHLYSIYPHG S TDKLPYVT MGSGSLAAMA VFEDKFRPDM EEEEAKNLVS EAIAAGIFND LGSGSNIDLC VISKNKLDFL RPYTVPNKKG TR LGRYRCE KGTTAVLTEK ITPLEIEVLE ETVQTMDTS

UniProtKB: Proteasome subunit beta type-7

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Macromolecule #29: Proteasome subunit beta type-3

MacromoleculeName: Proteasome subunit beta type-3 / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.841701 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SIMSYNGGAV MAMKGKNCVA IAADRRFGIQ AQMVTTDFQK IFPMGDRLYI GLAGLATDVQ TVAQRLKFRL NLYELKEGRQ IKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP D HLFETISQ ...String:
SIMSYNGGAV MAMKGKNCVA IAADRRFGIQ AQMVTTDFQK IFPMGDRLYI GLAGLATDVQ TVAQRLKFRL NLYELKEGRQ IKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP D HLFETISQ AMLNAVDRDA VSGMGVIVHI IEKDKITTRT LKARMD

UniProtKB: Proteasome subunit beta type-3

+
Macromolecule #30: Proteasome subunit beta type-2

MacromoleculeName: Proteasome subunit beta type-2 / type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.864277 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLA DCLRSRTPYH VNLLLAGYDE HEGPALYYMD YLAALAKAPF AAHGYGAFLT LSILDRYYTP TISRERAVEL L RKCLEELQ ...String:
MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLA DCLRSRTPYH VNLLLAGYDE HEGPALYYMD YLAALAKAPF AAHGYGAFLT LSILDRYYTP TISRERAVEL L RKCLEELQ KRFILNLPTF SVRIIDKNGI HDLDNISFPK QGS

UniProtKB: Proteasome subunit beta type-2

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Macromolecule #31: Proteasome subunit beta type-5

MacromoleculeName: Proteasome subunit beta type-5 / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 28.379053 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: ALASVLERPL PVNQRGFFGL GGRADLLDLG PGSLSDGLSL AAPGWGVPEE PGIEMLHGTT TLAFKFRHGV IVAADSRATA GAYIASQTV KKVIEINPYL LGTMAGGAAD CSFWERLLAR QCRIYELRNK ERISVAAASK LLANMVYQYK GMGLSMGTMI C GWDKRGPG ...String:
ALASVLERPL PVNQRGFFGL GGRADLLDLG PGSLSDGLSL AAPGWGVPEE PGIEMLHGTT TLAFKFRHGV IVAADSRATA GAYIASQTV KKVIEINPYL LGTMAGGAAD CSFWERLLAR QCRIYELRNK ERISVAAASK LLANMVYQYK GMGLSMGTMI C GWDKRGPG LYYVDSEGNR ISGATFSVGS GSVYAYGVMD RGYSYDLEVE QAYDLARRAI YQATYRDAYS GGAVNLYHVR ED GWIRVSS DNVADLHEKY SGSTP

UniProtKB: Proteasome subunit beta type-5

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Macromolecule #32: Proteasome subunit beta type-1

MacromoleculeName: Proteasome subunit beta type-1 / type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 26.391201 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: LSSTAMYSAP GRDLGMEPHR AAGPLQLRFS PYVFNGGTIL AIAGEDFAIV ASDTRLSEGF SIHTRDSPKC YKLTDKTVIG CSGFHGDCL TLTKIIEARL KMYKHSNNKA MTTGAIAAML STILYSRRFF PYYVYNIIGG LDEEGKGAVY SFDPVGSYQR D SFKAGGSA ...String:
LSSTAMYSAP GRDLGMEPHR AAGPLQLRFS PYVFNGGTIL AIAGEDFAIV ASDTRLSEGF SIHTRDSPKC YKLTDKTVIG CSGFHGDCL TLTKIIEARL KMYKHSNNKA MTTGAIAAML STILYSRRFF PYYVYNIIGG LDEEGKGAVY SFDPVGSYQR D SFKAGGSA SAMLQPLLDN QVGFKNMQNV EHVPLSLDRA MRLVKDVFIS AAERDVYTGD ALRICIVTKE GIREETVSLR KD

UniProtKB: Proteasome subunit beta type-1

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Macromolecule #33: Proteasome subunit beta type-4

MacromoleculeName: Proteasome subunit beta type-4 / type: protein_or_peptide / ID: 33 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.099986 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: EAFLGSRSGL WAGGPAPGQF YRIPSTPDSF MDPASALYRG PITRTQNPMV TGTSVLGVKF EGGVVIAADM LGSYGSLARF RNISRIMRV NNSTMLGASG DYADFQYLKQ VLGQMVIDEE LLGDGHSYSP RAIHSWLTRA MYSRRSKMNP LWNTMVIGGY A DGESFLGY ...String:
EAFLGSRSGL WAGGPAPGQF YRIPSTPDSF MDPASALYRG PITRTQNPMV TGTSVLGVKF EGGVVIAADM LGSYGSLARF RNISRIMRV NNSTMLGASG DYADFQYLKQ VLGQMVIDEE LLGDGHSYSP RAIHSWLTRA MYSRRSKMNP LWNTMVIGGY A DGESFLGY VDMLGVAYEA PSLATGYGAY LAQPLLREVL EKQPVLSQTE ARDLVERCMR VLYYRDARSY NRFQIATVTE KG VEIEGPL STETNWDIAH MISGFE

UniProtKB: Proteasome subunit beta type-4

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Macromolecule #34: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 34 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #35: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 35 / Number of copies: 4 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

+
Macromolecule #36: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 36 / Number of copies: 5 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #37: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 37 / Number of copies: 1 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

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Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
GridDetails: unspecified
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 44.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 348646
Initial angle assignmentType: COMMON LINE
Final angle assignmentType: PROJECTION MATCHING

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