[English] 日本語
Yorodumi
- EMDB-8668: Nucleotide-driven Triple-state Remodeling of the AAA-ATPase Chann... -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: EMDB / ID: EMD-8668
TitleNucleotide-driven Triple-state Remodeling of the AAA-ATPase Channel in the Activated Human 26S Proteasome
Map dataActivated Human 26S Proteasome
Sample
  • Complex: 26S proteasome bound to ATPgammaS
    • Protein or peptide: x 31 types
  • Protein or peptide: x 1 types
  • Ligand: x 1 types
Keywords26S proteasome / ATP-dependent protease / AAA-ATPase / peptide-unfolding channel / 20S core particle / HYDROLASE
Function / homology
Function and homology information


positive regulation of inclusion body assembly / thyrotropin-releasing hormone receptor binding / modulation by host of viral transcription / meiosis I / Hydrolases; Acting on peptide bonds (peptidases); Omega peptidases / proteasome accessory complex / purine ribonucleoside triphosphate binding / proteasome regulatory particle / cytosolic proteasome complex / positive regulation of proteasomal protein catabolic process ...positive regulation of inclusion body assembly / thyrotropin-releasing hormone receptor binding / modulation by host of viral transcription / meiosis I / Hydrolases; Acting on peptide bonds (peptidases); Omega peptidases / proteasome accessory complex / purine ribonucleoside triphosphate binding / proteasome regulatory particle / cytosolic proteasome complex / positive regulation of proteasomal protein catabolic process / proteasome regulatory particle, lid subcomplex / proteasome-activating activity / proteasome regulatory particle, base subcomplex / metal-dependent deubiquitinase activity / negative regulation of programmed cell death / regulation of endopeptidase activity / protein K63-linked deubiquitination / Regulation of ornithine decarboxylase (ODC) / proteasome core complex / Cross-presentation of soluble exogenous antigens (endosomes) / : / K63-linked deubiquitinase activity / Somitogenesis / myofibril / immune system process / proteasome binding / transcription factor binding / regulation of protein catabolic process / proteasome storage granule / blastocyst development / general transcription initiation factor binding / NF-kappaB binding / endopeptidase activator activity / polyubiquitin modification-dependent protein binding / proteasome assembly / protein deubiquitination / positive regulation of RNA polymerase II transcription preinitiation complex assembly / proteasome endopeptidase complex / proteasome core complex, beta-subunit complex / proteasome core complex, alpha-subunit complex / threonine-type endopeptidase activity / regulation of proteasomal protein catabolic process / enzyme regulator activity / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / inclusion body / ERAD pathway / sarcomere / proteasome complex / ciliary basal body / Regulation of activated PAK-2p34 by proteasome mediated degradation / proteolysis involved in protein catabolic process / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / Autodegradation of Cdh1 by Cdh1:APC/C / APC/C:Cdc20 mediated degradation of Securin / Asymmetric localization of PCP proteins / SCF-beta-TrCP mediated degradation of Emi1 / NIK-->noncanonical NF-kB signaling / Ubiquitin-dependent degradation of Cyclin D / AUF1 (hnRNP D0) binds and destabilizes mRNA / TNFR2 non-canonical NF-kB pathway / stem cell differentiation / Vpu mediated degradation of CD4 / Assembly of the pre-replicative complex / Degradation of DVL / negative regulation of inflammatory response to antigenic stimulus / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / Dectin-1 mediated noncanonical NF-kB signaling / lipopolysaccharide binding / Hh mutants are degraded by ERAD / Degradation of AXIN / Activation of NF-kappaB in B cells / Degradation of GLI1 by the proteasome / Hedgehog ligand biogenesis / Defective CFTR causes cystic fibrosis / G2/M Checkpoints / Negative regulation of NOTCH4 signaling / GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 / Autodegradation of the E3 ubiquitin ligase COP1 / Vif-mediated degradation of APOBEC3G / double-strand break repair via homologous recombination / P-body / Regulation of RUNX3 expression and activity / Hedgehog 'on' state / Degradation of GLI2 by the proteasome / GLI3 is processed to GLI3R by the proteasome / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / MAPK6/MAPK4 signaling / APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 / response to virus / Degradation of beta-catenin by the destruction complex / ABC-family proteins mediated transport / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / response to organic cyclic compound / CDK-mediated phosphorylation and removal of Cdc6 / Metalloprotease DUBs / CLEC7A (Dectin-1) signaling / SCF(Skp2)-mediated degradation of p27/p21 / double-strand break repair via nonhomologous end joining / Regulation of expression of SLITs and ROBOs
Similarity search - Function
: / Ubiquitin interaction motif / : / 26S proteasome regulatory subunit RPN7/PSMD6 C-terminal helix / 26S proteasome non-ATPase regulatory subunit Rpn12 / 26S proteasome regulatory subunit, C-terminal / Proteasome regulatory subunit C-terminal / 26S proteasome regulatory subunit RPN5, C-terminal domain / : / 26S proteasome regulatory subunit RPN5 C-terminal domain ...: / Ubiquitin interaction motif / : / 26S proteasome regulatory subunit RPN7/PSMD6 C-terminal helix / 26S proteasome non-ATPase regulatory subunit Rpn12 / 26S proteasome regulatory subunit, C-terminal / Proteasome regulatory subunit C-terminal / 26S proteasome regulatory subunit RPN5, C-terminal domain / : / 26S proteasome regulatory subunit RPN5 C-terminal domain / PSD13 N-terminal repeats / 26S proteasome regulatory subunit Rpn6, N-terminal / 6S proteasome subunit Rpn6, C-terminal helix domain / 26S proteasome regulatory subunit RPN6 N-terminal domain / 26S proteasome subunit RPN6 C-terminal helix domain / 26S Proteasome non-ATPase regulatory subunit 13 / : / 26S proteasome subunit RPN2, N-terminal domain / 26S proteasome regulatory complex, non-ATPase subcomplex, Rpn2/Psmd1 subunit / 26S proteasome regulatory subunit RPN2, C-terminal / 26S proteasome regulatory subunit RPN2 C-terminal domain / 26S Proteasome non-ATPase regulatory subunit 7/8 / 26S proteasome regulatory complex, non-ATPase subcomplex, Rpn1 subunit / RPN1, N-terminal / 26S proteasome non-ATPase regulatory subunit RPN1, C-terminal / : / RPN1 N-terminal domain / 26S proteasome non-ATPase regulatory subunit RPN1 C-terminal / 26S proteasome regulatory subunit 7, OB domain / : / : / PSMD12/CSN4, N-terminal / 26S proteasome regulatory subunit Rpn7/COP9 signalosome complex subunit 1 / 26S proteasome regulatory subunit Rpn7, N-terminal / 26S proteasome subunit RPN7 / 26S Proteasome non-ATPase regulatory subunit 12/COP9 signalosome complex subunit 4 / Proteasome/cyclosome repeat / Ubiquitin-interacting motif. / Proteasome/cyclosome repeat / PCI/PINT associated module / : / von Willebrand factor type A domain / Proteasome subunit alpha 1 / HEAT repeats / Proteasomal ATPase OB C-terminal domain / Proteasomal ATPase OB C-terminal domain / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / Rpn11/EIF3F, C-terminal / Maintenance of mitochondrial structure and function / : / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / Ubiquitin interacting motif / Ubiquitin-interacting motif (UIM) domain profile. / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / Proteasome beta subunit, C-terminal / Proteasome beta subunits C terminal / Proteasome subunit beta 4 / Proteasome subunit beta 2 / Proteasome beta 3 subunit / Proteasome subunit alpha6 / Proteasome subunit alpha5 / Proteasome beta-type subunits signature. / Peptidase T1A, proteasome beta-subunit / Proteasome beta-type subunit, conserved site / Proteasome subunit A N-terminal signature / Proteasome alpha-type subunits signature. / Proteasome alpha-subunit, N-terminal domain / Proteasome subunit A N-terminal signature Add an annotation / von Willebrand factor (vWF) type A domain / : / Proteasome alpha-type subunit / Proteasome alpha-type subunit profile. / VWFA domain profile. / Proteasome B-type subunit / Proteasome beta-type subunit profile. / Proteasome subunit / Proteasome, subunit alpha/beta / von Willebrand factor, type A / AAA ATPase, AAA+ lid domain / AAA+ lid domain / ATPase, AAA-type, conserved site / AAA-protein family signature. / JAB/MPN domain / JAB1/MPN/MOV34 metalloenzyme domain / MPN domain / MPN domain profile. / Nucleophile aminohydrolases, N-terminal / von Willebrand factor A-like domain superfamily / ATPase family associated with various cellular activities (AAA) / ATPase, AAA-type, core / Armadillo-like helical / Tetratricopeptide-like helical domain superfamily / Armadillo-type fold / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily / ATPases associated with a variety of cellular activities
Similarity search - Domain/homology
26S proteasome non-ATPase regulatory subunit 11 / 26S proteasome non-ATPase regulatory subunit 12 / 26S proteasome non-ATPase regulatory subunit 14 / Proteasome subunit alpha type-7 / 26S proteasome non-ATPase regulatory subunit 3 / 26S proteasome regulatory subunit 6A / Proteasome subunit beta type-1 / Proteasome subunit alpha type-1 / Proteasome subunit alpha type-2 / Proteasome subunit alpha type-3 ...26S proteasome non-ATPase regulatory subunit 11 / 26S proteasome non-ATPase regulatory subunit 12 / 26S proteasome non-ATPase regulatory subunit 14 / Proteasome subunit alpha type-7 / 26S proteasome non-ATPase regulatory subunit 3 / 26S proteasome regulatory subunit 6A / Proteasome subunit beta type-1 / Proteasome subunit alpha type-1 / Proteasome subunit alpha type-2 / Proteasome subunit alpha type-3 / Proteasome subunit alpha type-4 / Proteasome subunit alpha type-5 / Proteasome subunit beta type-4 / Proteasome subunit beta type-6 / Proteasome subunit beta type-5 / 26S proteasome regulatory subunit 7 / 26S proteasome regulatory subunit 6B / 26S proteasome non-ATPase regulatory subunit 8 / Proteasome subunit beta type-3 / Proteasome subunit beta type-2 / 26S proteasome non-ATPase regulatory subunit 7 / 26S proteasome non-ATPase regulatory subunit 4 / Proteasome subunit alpha type-6 / 26S proteasome regulatory subunit 4 / 26S proteasome regulatory subunit 8 / 26S proteasome regulatory subunit 10B / 26S proteasome non-ATPase regulatory subunit 2 / 26S proteasome non-ATPase regulatory subunit 6 / Proteasome subunit beta type-7 / 26S proteasome non-ATPase regulatory subunit 1 / 26S proteasome non-ATPase regulatory subunit 13
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.8 Å
AuthorsZhu Y / Wang WL
CitationJournal: Nat Commun / Year: 2018
Title: Structural mechanism for nucleotide-driven remodeling of the AAA-ATPase unfoldase in the activated human 26S proteasome.
Authors: Yanan Zhu / Wei Li Wang / Daqi Yu / Qi Ouyang / Ying Lu / Youdong Mao /
Abstract: The proteasome is a sophisticated ATP-dependent molecular machine responsible for protein degradation in all known eukaryotic cells. It remains elusive how conformational changes of the AAA-ATPase ...The proteasome is a sophisticated ATP-dependent molecular machine responsible for protein degradation in all known eukaryotic cells. It remains elusive how conformational changes of the AAA-ATPase unfoldase in the regulatory particle (RP) control the gating of the substrate-translocation channel leading to the proteolytic chamber of the core particle (CP). Here we report three alternative states of the ATP-γ-S-bound human proteasome, in which the CP gates are asymmetrically open, visualized by cryo-EM at near-atomic resolutions. At least four nucleotides are bound to the AAA-ATPase ring in these open-gate states. Variation in nucleotide binding gives rise to an axial movement of the pore loops narrowing the substrate-translation channel, which exhibit remarkable structural transitions between the spiral-staircase and saddle-shaped-circle topologies. Gate opening in the CP is thus regulated by nucleotide-driven conformational changes of the AAA-ATPase unfoldase. These findings demonstrate an elegant mechanism of allosteric coordination among sub-machines within the human proteasome holoenzyme.
History
DepositionApr 9, 2017-
Header (metadata) releaseJul 18, 2018-
Map releaseJul 18, 2018-
UpdateOct 23, 2024-
Current statusOct 23, 2024Processing site: RCSB / Status: Released

-
Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0038
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 0.0038
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-5vfu
  • Surface level: 0.0038
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_8668.png

Downloads & links

-
Map

FileDownload / File: emd_8668.map.gz / Format: CCP4 / Size: 669.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationActivated Human 26S Proteasome
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.75 Å/pix.
x 560 pix.
= 420. Å		0.75 Å/pix.
x 560 pix.
= 420. Å		0.75 Å/pix.
x 560 pix.
= 420. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.75 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.004 / Movie #1: 0.0038
Minimum - Maximum-0.0039869426 - 0.008534854
Average (Standard dev.)0.000013071547 (±0.00076903583)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions560560560
Spacing560560560
CellA=B=C: 420.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.750.750.75
M x/y/z560560560
origin x/y/z0.0000.0000.000
length x/y/z420.000420.000420.000
α/β/γ90.00090.00090.000
start NX/NY/NZ-152-37
NX/NY/NZ998271
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS560560560
D min/max/mean-0.0040.0090.000

-
Supplemental data

-
Additional map: Uncorrected raw map

Fileemd_8668_additional.map
AnnotationUncorrected raw map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

-
Sample components

+
Entire : 26S proteasome bound to ATPgammaS

EntireName: 26S proteasome bound to ATPgammaS
Components
  • Complex: 26S proteasome bound to ATPgammaS
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 1
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 3
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 12
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 11
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 6
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 7
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 13
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 4
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 8
    • Protein or peptide: Sem1
    • Protein or peptide: 26S protease regulatory subunit 7
    • Protein or peptide: 26S protease regulatory subunit 4
    • Protein or peptide: 26S protease regulatory subunit 8
    • Protein or peptide: 26S protease regulatory subunit 6B
    • Protein or peptide: 26S protease regulatory subunit 10B
    • Protein or peptide: 26S protease regulatory subunit 6A
    • Protein or peptide: Proteasome subunit alpha type-6
    • Protein or peptide: Proteasome subunit alpha type-2
    • Protein or peptide: Proteasome subunit alpha type-4
    • Protein or peptide: Proteasome subunit alpha type-7
    • Protein or peptide: Proteasome subunit alpha type-5
    • Protein or peptide: Proteasome subunit alpha type-1
    • Protein or peptide: Proteasome subunit alpha type-3
    • Protein or peptide: Proteasome subunit beta type-6
    • Protein or peptide: Proteasome subunit beta type-7
    • Protein or peptide: Proteasome subunit beta type-3
    • Protein or peptide: Proteasome subunit beta type-2
    • Protein or peptide: Proteasome subunit beta type-5
    • Protein or peptide: Proteasome subunit beta type-1
    • Protein or peptide: Proteasome subunit beta type-4
    • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 2
  • Protein or peptide: 26S proteasome non-ATPase regulatory subunit 14
  • Ligand: ZINC ION

+
Supramolecule #1: 26S proteasome bound to ATPgammaS

SupramoleculeName: 26S proteasome bound to ATPgammaS / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#8, #10-#32
Source (natural)Organism: Homo sapiens (human)

+
Macromolecule #1: 26S proteasome non-ATPase regulatory subunit 1

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 101.26318 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: GIISLLDEDE PQLKEFALHK LNAVVNDFWA EISESVDKIE VLYEDEGFRS RQFAALVASK VFYHLGAFEE SLNYALGAGD LFNVNDNSE YVETIIAKCI DHYTKQCVEN ADLPEGEKKP IDQRLEGIVN KMFQRCLDDH KYKQAIGIAL ETRRLDVFEK T ILESNDVP ...String:
GIISLLDEDE PQLKEFALHK LNAVVNDFWA EISESVDKIE VLYEDEGFRS RQFAALVASK VFYHLGAFEE SLNYALGAGD LFNVNDNSE YVETIIAKCI DHYTKQCVEN ADLPEGEKKP IDQRLEGIVN KMFQRCLDDH KYKQAIGIAL ETRRLDVFEK T ILESNDVP GMLAYSLKLC MSLMQNKQFR NKVLRVLVKI YMNLEKPDFI NVCQCLIFLD DPQAVSDILE KLVKEDNLLM AY QICFDLY ESASQQFLSS VIQNLRTVGT PIASVPGSTN TGTVPGSEKD SDSMETEEKT SSAFVGKTPE ASPEPKDQTL KMI KILSGE MAIELHLQFL IRNNNTDLMI LKNTKDAVRN SVCHTATVIA NSFMHCGTTS DQFLRDNLEW LARATNWAKF TATA SLGVI HKGHEKEALQ LMATYLPKDT SPGSAYQEGG GLYALGLIHA NHGGDIIDYL LNQLKNASND IVRHGGSLGL GLAAM GTAR QDVYDLLKTN LYQDDAVTGE AAGLALGLVM LGSKNAQAIE DMVGYAQETQ HEKILRGLAV GIALVMYGRM EEADAL IES LCRDKDPILR RSGMYTVAMA YCGSGNNKAI RRLLHVAVSD VNDDVRRAAV ESLGFILFRT PEQCPSVVSL LSESYNP HV RYGAAMALGI CCAGTGNKEA INLLEPMTND PVNYVRQGAL IASALIMIQQ TEITCPKVNQ FRQLYSKVIN DKHDDVMA K FGAILAQGIL DAGGHNVTIS LQSRTGHTHM PSVVGVLVFT QFWFWFPLSH FLSLAYTPTC VIGLNKDLKM PKVQYKSNC KPSTFAYPAP LEVPKEKEKE KVSTAVLSIT AKAKKKEKEK EKKEEEKMEV DEAEKKEEKE KKKEPEPNFQ LLDNPARVMP AQLKVLTMP ETCRYQPFKP LSIGGIIILK DT

UniProtKB: 26S proteasome non-ATPase regulatory subunit 1

+
Macromolecule #2: 26S proteasome non-ATPase regulatory subunit 3

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 54.755656 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: PPGGGEQEPP PPPAPQDVEM KEEAATGGGS TGEADGKTAA AAAEHSQREL DTVTLEDIKE HVKQLEKAVS GKEPRFVLRA LRMLPSTSR RLNHYVLYKA VQGFFTSNNA TRDFLLPFLE EPMDTEADLQ FRPRTGKAAS TPLLPEVEAY LQLLVVIFMM N SKRYKEAQ ...String:
PPGGGEQEPP PPPAPQDVEM KEEAATGGGS TGEADGKTAA AAAEHSQREL DTVTLEDIKE HVKQLEKAVS GKEPRFVLRA LRMLPSTSR RLNHYVLYKA VQGFFTSNNA TRDFLLPFLE EPMDTEADLQ FRPRTGKAAS TPLLPEVEAY LQLLVVIFMM N SKRYKEAQ KISDDLMQKI STQNRRALDL VAAKCYYYHA RVYEFLDKLD VVRSFLHARL RTATLRHDAD GQATLLNLLL RN YLHYSLY DQAEKLVSKS VFPEQANNNE WARYLYYTGR IKAIQLEYSE ARRTMTNALR KAPQHTAVGF KQTVHKLLIV VEL LLGEIP DRLQFRQPSL KRSLMPYFLL TQAVRTGNLA KFNQVLDQFG EKFQADGTYT LIIRLRHNVI KTGVRMISLS YSRI SLADI AQKLQLDSPE DAEFIVAKAI RDGVIEASIN HEKGYVQSKE MIDIYSTREP QLAFHQRISF CLDIHNMSVK AMRFP

UniProtKB: 26S proteasome non-ATPase regulatory subunit 3

+
Macromolecule #3: 26S proteasome non-ATPase regulatory subunit 12

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 12 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 52.979359 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MADGGSERAD GRIVKMEVDY SATVDQRLPE CAKLAKEGRL QEVIETLLSL EKQTRTASDM VSTSRILVAV VKMCYEAKEW DLLNENIML LSKRRSQLKQ AVAKMVQQCC TYVEEITDLP IKLRLIDTLR MVTEGKIYVE IERARLTKTL ATIKEQNGDV K EAASILQE ...String:
MADGGSERAD GRIVKMEVDY SATVDQRLPE CAKLAKEGRL QEVIETLLSL EKQTRTASDM VSTSRILVAV VKMCYEAKEW DLLNENIML LSKRRSQLKQ AVAKMVQQCC TYVEEITDLP IKLRLIDTLR MVTEGKIYVE IERARLTKTL ATIKEQNGDV K EAASILQE LQVETYGSME KKERVEFILE QMRLCLAVKD YIRTQIISKK INTKFFQEEN TEKLKLKYYN LMIQLDQHEG SY LSICKHY RAIYDTPCIQ AESEKWQQAL KSVVLYVILA PFDNEQSDLV HRISGDKKLE EIPKYKDLLK LFTTMELMRW STL VEDYGM ELRKGSLESP ATDVFGSTEE GEKRWKDLKN RVVEHNIRIM AKYYTRITMK RMAQLLDLSV DESEAFLSNL VVNK TIFAK VDRLAGIINF QRPKDPNNLL NDWSQKLNSL MSLVNKTTHL IAKEEMIHNL Q

UniProtKB: 26S proteasome non-ATPase regulatory subunit 12

+
Macromolecule #4: 26S proteasome non-ATPase regulatory subunit 11

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 11 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 42.868555 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: VQVKEQSILE LGSLLAKTGQ AAELGGLLKY VRPFLNSISK AKAARLVRSL LDLFLDMEAA TGQEVELCLE CIEWAKSEKR TFLRQALEA RLVSLYFDTK RYQEALHLGS QLLRELKKMD DKALLVEVQL LESKTYHALS NLPKARAALT SARTTANAIY C PPKLQATL ...String:
VQVKEQSILE LGSLLAKTGQ AAELGGLLKY VRPFLNSISK AKAARLVRSL LDLFLDMEAA TGQEVELCLE CIEWAKSEKR TFLRQALEA RLVSLYFDTK RYQEALHLGS QLLRELKKMD DKALLVEVQL LESKTYHALS NLPKARAALT SARTTANAIY C PPKLQATL DMQSGIIHAA EEKDWKTAYS YFYEAFEGYD SIDSPKAITS LKYMLLCKIM LNTPEDVQAL VSGKLALRYA GR QTEALKC VAQASKNRSL ADFEKALTDY RAELRDDPII STHLAKLYDN LLEQNLIRVI EPFSRVQIEH ISSLIKLSKA DVE RKLSQM ILDKKFHGIL DQGEGVLIIF DEPPVDKTYE AALETIQNMS KVVDSLYNKA KKLT

UniProtKB: 26S proteasome non-ATPase regulatory subunit 11

+
Macromolecule #5: 26S proteasome non-ATPase regulatory subunit 6

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 44.336906 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: PKNPDLRIAQ LRFLLSLPEH RGDAAVRDEL MAAVRDNNMA PYYEALCKSL DWQIDVDLLN KMKKANEDEL KRLDEELEDA EKNLGESEI RDAMMAKAEY LCRIGDKEGA LTAFRKTYDK TVALGHRLDI VFYLLRIGLF YMDNDLITRN TEKAKSLIEE G GDWDRRNR ...String:
PKNPDLRIAQ LRFLLSLPEH RGDAAVRDEL MAAVRDNNMA PYYEALCKSL DWQIDVDLLN KMKKANEDEL KRLDEELEDA EKNLGESEI RDAMMAKAEY LCRIGDKEGA LTAFRKTYDK TVALGHRLDI VFYLLRIGLF YMDNDLITRN TEKAKSLIEE G GDWDRRNR LKVYQGLYCV AIRDFKQAAE LFLDTVSTFT SYELMDYKTF VTYTVYVSMI ALERPDLREK VIKGAEILEV LH SLPAVRQ YLFSLYECRY SVFFQSLAVV EQEMKKDWLF APHYRYYVRE MRIHAYSQLL ESYRSLTLGY MAEAFGVGVE FID QELSRF IAAGRLHCKI DKVNEIVETN RPDSKNWQYQ ETIKKGDLLL NRVQKLSRVI NM

UniProtKB: 26S proteasome non-ATPase regulatory subunit 6

+
Macromolecule #6: 26S proteasome non-ATPase regulatory subunit 7

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 7 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 32.382094 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: AVQKVVVHPL VLLSVVDHFN RIGKVGNQKR VVGVLLGSWQ KKVLDVSNSF AVPFDEDDKD DSVWFLDHDY LENMYGMFKK VNARERIVG WYHTGPKLHK NDIAINELMK RYCPNSVLVI IDVKPKDLGL PTEAYISVEE VHDDGTPTSK TFEHVTSEIG A EEAEEVGV ...String:
AVQKVVVHPL VLLSVVDHFN RIGKVGNQKR VVGVLLGSWQ KKVLDVSNSF AVPFDEDDKD DSVWFLDHDY LENMYGMFKK VNARERIVG WYHTGPKLHK NDIAINELMK RYCPNSVLVI IDVKPKDLGL PTEAYISVEE VHDDGTPTSK TFEHVTSEIG A EEAEEVGV EHLLRDIKDT TVGTLSQRIT NQVHGLKGLN SKLLDIRSYL EKVATGKLPI NHQIIYQLQD VFNLLPDVSL QE FVKAFYL KTNDQMVVVY LASLIRSVVA LHNLINNKIA NRDAEKKEG

UniProtKB: 26S proteasome non-ATPase regulatory subunit 7

+
Macromolecule #7: 26S proteasome non-ATPase regulatory subunit 13

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 13 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 42.592875 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: VPGFLQQSQS SGPGQPAVWH RLEELYTKKL WHQLTLQVLD FVQDPCFAQG DGLIKLYENF ISEFEHRVNP LSLVEIILHV VRQMTDPNV ALTFLEKTRE KVKSSDEAVI LCKTAIGALK LNIGDLQVTK ETIEDVEEML NNLPGVTSVH SRFYDLSSKY Y QTIGNHAS ...String:
VPGFLQQSQS SGPGQPAVWH RLEELYTKKL WHQLTLQVLD FVQDPCFAQG DGLIKLYENF ISEFEHRVNP LSLVEIILHV VRQMTDPNV ALTFLEKTRE KVKSSDEAVI LCKTAIGALK LNIGDLQVTK ETIEDVEEML NNLPGVTSVH SRFYDLSSKY Y QTIGNHAS YYKDALRFLG CVDIKDLPVS EQQERAFTLG LAGLLGEGVF NFGELLMHPV LESLRNTDRQ WLIDTLYAFN SG NVERFQT LKTAWGQQPD LAANEAQLLR KIQLLCLMEM TFTRPANHRQ LTFEEIAKSA KITVNEVELL VMKALSVGLV KGS IDEVDK RVHMTWVQPR VLDLQQIKGM KDRLEFWCTD VKSMEMLVEH QAHDILT

UniProtKB: 26S proteasome non-ATPase regulatory subunit 13

+
Macromolecule #8: 26S proteasome non-ATPase regulatory subunit 4

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 4 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.866023 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MVLESTMVCV DNSEYMRNGD FLPTRLQAQQ DAVNIVCHSK TRSNPENNVG LITLANDCEV LTTLTPDTGR ILSKLHTVQP KGKITFCTG IRVAHLALKH RQGKNHKMRI IAFVGSPVED NEKDLVKLAK RLKKEKVNVD IINFGEEEVN TEKLTAFVNT L NGKDGTGS HLVTVPPGPS LADALISSPI LAG

UniProtKB: 26S proteasome non-ATPase regulatory subunit 4

+
Macromolecule #9: 26S proteasome non-ATPase regulatory subunit 14

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 14 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
EC number: Hydrolases; Acting on peptide bonds (peptidases); Omega peptidases
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 32.329355 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: AVDTAEQVYI SSLALLKMLK HGRAGVPMEV MGLMLGEFVD DYTVRVIDVF AMPQSGTGVS VEAVDPVFQA KMLDMLKQTG RPEMVVGWY HSHPGFGCWL SGVDINTQQS FEALSERAVA VVVDPIQSVK GKVVIDAFRL INANMMVLGH EPRQTTSNLG H LNKPSIQA ...String:
AVDTAEQVYI SSLALLKMLK HGRAGVPMEV MGLMLGEFVD DYTVRVIDVF AMPQSGTGVS VEAVDPVFQA KMLDMLKQTG RPEMVVGWY HSHPGFGCWL SGVDINTQQS FEALSERAVA VVVDPIQSVK GKVVIDAFRL INANMMVLGH EPRQTTSNLG H LNKPSIQA LIHGLNRHYY SITINYRKNE LEQKMLLNLH KKSWMEGLTL QDYSEHCKHN ESVVKEMLEL AKNYNKAVEE ED KMTPEQL AIKNVGKQDP KRHLEEHVDV LMTSNIVQCL AAMLDTVVFK

UniProtKB: 26S proteasome non-ATPase regulatory subunit 14

+
Macromolecule #10: 26S proteasome non-ATPase regulatory subunit 8

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 8 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 30.039699 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MYEQLKGEWN RKSPNLSKCG EELGRLKLVL LELNFLPTTG TKLTKQQLIL ARDILEIGAQ WSILRKDIPS FERYMAQLKC YYFDYKEQL PESAYMHQLL GLNLLFLLSQ NRVAEFHTEL ERLPAKDIQT NVYIKHPVSL EQYLMEGSYN KVFLAKGNIP A ESYTFFID ...String:
MYEQLKGEWN RKSPNLSKCG EELGRLKLVL LELNFLPTTG TKLTKQQLIL ARDILEIGAQ WSILRKDIPS FERYMAQLKC YYFDYKEQL PESAYMHQLL GLNLLFLLSQ NRVAEFHTEL ERLPAKDIQT NVYIKHPVSL EQYLMEGSYN KVFLAKGNIP A ESYTFFID ILLDTIRDEI AGCIEKAYEK ILFTEATRIL FFNTPKKMTD YAKKRGWVLG PNNYYSFASQ QQKPEDTTIP ST ELAKQVI EYARQLEMIV

UniProtKB: 26S proteasome non-ATPase regulatory subunit 8

+
Macromolecule #11: Sem1

MacromoleculeName: Sem1 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 8.284611 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
MSEKKQPVDL GLLEEDDEFE EFPAEDWAGL DEDEDAHVWE DNWDDDNVED DFSNQLRAEL EKHGYKMETS

+
Macromolecule #12: 26S protease regulatory subunit 7

MacromoleculeName: 26S protease regulatory subunit 7 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 40.494668 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: APPALWDLAA DKQTLQSEQP LQVARCTKII NADSEDPKYI INVKQFAKFV VDLSDQVAPT DIEEGMRVGV DRNKYQIHIP LPPKIDPTV TMMQVEEKPD VTYSDVGGCK EQIEKLREVV ETPLLHPERF VNLGIEPPKG VLLFGPPGTG KTLCARAVAN R TDACFIRV ...String:
APPALWDLAA DKQTLQSEQP LQVARCTKII NADSEDPKYI INVKQFAKFV VDLSDQVAPT DIEEGMRVGV DRNKYQIHIP LPPKIDPTV TMMQVEEKPD VTYSDVGGCK EQIEKLREVV ETPLLHPERF VNLGIEPPKG VLLFGPPGTG KTLCARAVAN R TDACFIRV IGSELVQKYV GEGARMVREL FEMARTKKAC LIFFDEIDAI GGARFDDGAG GDNEVQRTML ELINQLDGFD PR GNIKVLM ATNRPDTLDP ALMRPGRLDR KIEFSLPDLE GRTHIFKIHA RSMSVERDIR FELLARLCPN STGAEIRSVC TEA GMFAIR ARRKIATEKD FLEAVNKVIK SYAKFSATPR YMTYN

UniProtKB: 26S proteasome regulatory subunit 7

+
Macromolecule #13: 26S protease regulatory subunit 4

MacromoleculeName: 26S protease regulatory subunit 4 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 38.799266 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: EEERSKVDDL RGTPMSVGTL EEIIDDNHAI VSTSVGSEHY VSILSFVDKD LLEPGCSVLL NHKVHAVIGV LMDDTDPLVT VMKVEKAPQ ETYADIGGLD NQIQEIKESV ELPLTHPEYY EEMGIKPPKG VILYGPPGTG KTLLAKAVAN QTSATFLRVV G SELIQKYL ...String:
EEERSKVDDL RGTPMSVGTL EEIIDDNHAI VSTSVGSEHY VSILSFVDKD LLEPGCSVLL NHKVHAVIGV LMDDTDPLVT VMKVEKAPQ ETYADIGGLD NQIQEIKESV ELPLTHPEYY EEMGIKPPKG VILYGPPGTG KTLLAKAVAN QTSATFLRVV G SELIQKYL GDGPKLVREL FRVAEEHAPS IVFIDEIDAI GTKRYDSNSG GEREIQRTML ELLNQLDGFD SRGDVKVIMA TN RIETLDP ALIRPGRIDR KIEFPLPDEK TKKRIFQIHT SRMTLADDVT LDDLIMAKDD LSGADIKAIC TEAGLMALRE RRM KVTNED FKKSKENVLY KKQEGTPEGL YL

UniProtKB: 26S proteasome regulatory subunit 4

+
Macromolecule #14: 26S protease regulatory subunit 8

MacromoleculeName: 26S protease regulatory subunit 8 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 43.114 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: LEEGKAGSGL RQYYLSKIEE LQLIVNDKSQ NLRRLQAQRN ELNAKVRLLR EELQLLQEQG SYVGEVVRAM DKKKVLVKVH PEGKFVVDV DKNIDINDVT PNCRVALRND SYTLHKILPN KVDPLVSLMM VEKVPDSTYE MIGGLDKQIK EIKEVIELPV K HPELFEAL ...String:
LEEGKAGSGL RQYYLSKIEE LQLIVNDKSQ NLRRLQAQRN ELNAKVRLLR EELQLLQEQG SYVGEVVRAM DKKKVLVKVH PEGKFVVDV DKNIDINDVT PNCRVALRND SYTLHKILPN KVDPLVSLMM VEKVPDSTYE MIGGLDKQIK EIKEVIELPV K HPELFEAL GIAQPKGVLL YGPPGTGKTL LARAVAHHTD CTFIRVSGSE LVQKFIGEGA RMVRELFVMA REHAPSIIFM DE IDSIGSS RLEGGSGGDS EVQRTMLELL NQLDGFEATK NIKVIMATNR IDILDSALLR PGRIDRKIEF PPPNEEARLD ILK IHSRKM NLTRGINLRK IAELMPGASG AEVKGVCTEA GMYALRERRV HVTQEDFEMA VAKVMQKD

UniProtKB: 26S proteasome regulatory subunit 8

+
Macromolecule #15: 26S protease regulatory subunit 6B

MacromoleculeName: 26S protease regulatory subunit 6B / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 43.303543 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: DLYSRYKKLQ QELEFLEVQE EYIKDEQKNL KKEFLHAQEE VKRIQSIPLV IGQFLEAVDQ NTAIVGSTTG SNYYVRILST IDRELLKPN ASVALHKHSN ALVDVLPPEA DSSIMMLTSD QKPDVMYADI GGMDIQKQEV REAVELPLTH FELYKQIGID P PRGVLMYG ...String:
DLYSRYKKLQ QELEFLEVQE EYIKDEQKNL KKEFLHAQEE VKRIQSIPLV IGQFLEAVDQ NTAIVGSTTG SNYYVRILST IDRELLKPN ASVALHKHSN ALVDVLPPEA DSSIMMLTSD QKPDVMYADI GGMDIQKQEV REAVELPLTH FELYKQIGID P PRGVLMYG PPGCGKTMLA KAVAHHTTAA FIRVVGSEFV QKYLGEGPRM VRDVFRLAKE NAPAIIFIDE IDAIATKRFD AQ TGADREV QRILLELLNQ MDGFDQNVNV KVIMATNRAD TLDPALLRPG RLDRKIEFPL PDRRQKRLIF STITSKMNLS EEV DLEDYV ARPDKISGAD INSICQESGM LAVRENRYIV LAKDFEKAYK TVIKKDEQEH EFYK

UniProtKB: 26S proteasome regulatory subunit 6B

+
Macromolecule #16: 26S protease regulatory subunit 10B

MacromoleculeName: 26S protease regulatory subunit 10B / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 39.840891 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: TKQYEKSEND LKALQSVGQI VGEVLKQLTE EKFIVKATNG PRYVVGCRRQ LDKSKLKPGT RVALDMTTLT IMRYLPREVD PLVYNMSHE DPGNVSYSEI GGLSEQIREL REVIELPLTN PELFQRVGII PPKGCLLYGP PGTGKTLLAR AVASQLDCNF L KVVSSSIV ...String:
TKQYEKSEND LKALQSVGQI VGEVLKQLTE EKFIVKATNG PRYVVGCRRQ LDKSKLKPGT RVALDMTTLT IMRYLPREVD PLVYNMSHE DPGNVSYSEI GGLSEQIREL REVIELPLTN PELFQRVGII PPKGCLLYGP PGTGKTLLAR AVASQLDCNF L KVVSSSIV DKYIGESARL IREMFNYARD HQPCIIFMDE IDAIGGRRFS EGTSADREIQ RTLMELLNQM DGFDTLHRVK MI MATNRPD TLDPALLRPG RLDRKIHIDL PNEQARLDIL KIHAGPITKH GEIDYEAIVK LSDGFNGADL RNVCTEAGMF AIR ADHDFV VQEDFMKAVR KVADSKKLES KLDYKPV

UniProtKB: 26S proteasome regulatory subunit 10B

+
Macromolecule #17: 26S protease regulatory subunit 6A

MacromoleculeName: 26S protease regulatory subunit 6A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 42.104242 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: THELQAMKDK IKENSEKIKV NKTLPYLVSN VIELLDVDPN DQEEDGANID LDSQRKGKCA VIKTSTRQTY FLPVIGLVDA EKLKPGDLV GVNKDSYLIL ETLPTEYDSR VKAMEVDERP TEQYSDIGGL DKQIQELVEA IVLPMNHKEK FENLGIQPPK G VLMYGPPG ...String:
THELQAMKDK IKENSEKIKV NKTLPYLVSN VIELLDVDPN DQEEDGANID LDSQRKGKCA VIKTSTRQTY FLPVIGLVDA EKLKPGDLV GVNKDSYLIL ETLPTEYDSR VKAMEVDERP TEQYSDIGGL DKQIQELVEA IVLPMNHKEK FENLGIQPPK G VLMYGPPG TGKTLLARAC AAQTKATFLK LAGPQLVQMF IGDGAKLVRD AFALAKEKAP SIIFIDELDA IGTKRFDSEK AG DREVQRT MLELLNQLDG FQPNTQVKVI AATNRVDILD PALLRSGRLD RKIEFPMPNE EARARIMQIH SRKMNVSPDV NYE ELARCT DDFNGAQCKA VCVEAGMIAL RRGATELTHE DYMEGILEVQ AKKKANLQYY A

UniProtKB: 26S proteasome regulatory subunit 6A

+
Macromolecule #18: Proteasome subunit alpha type-6

MacromoleculeName: Proteasome subunit alpha type-6 / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 26.727658 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SSAGFDRHIT IFSPEGRLYQ VEYAFKAINQ GGLTSVAVRG KDCAVIVTQK KVPDKLLDSS TVTHLFKITE NIGCVMTGMT ADSRSQVQR ARYEAANWKY KYGYEIPVDM LCKRIADISQ VYTQNAEMRP LGCCMILIGI DEEQGPQVYK CDPAGYYCGF K ATAAGVKQ ...String:
SSAGFDRHIT IFSPEGRLYQ VEYAFKAINQ GGLTSVAVRG KDCAVIVTQK KVPDKLLDSS TVTHLFKITE NIGCVMTGMT ADSRSQVQR ARYEAANWKY KYGYEIPVDM LCKRIADISQ VYTQNAEMRP LGCCMILIGI DEEQGPQVYK CDPAGYYCGF K ATAAGVKQ TESTSFLEKK VKKKFDWTFE QTVETAITCL STVLSIDFKP SEIEVGVVTV ENPKFRILTE AEIDAHLVAL AE

UniProtKB: Proteasome subunit alpha type-6

+
Macromolecule #19: Proteasome subunit alpha type-2

MacromoleculeName: Proteasome subunit alpha type-2 / type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.72526 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: ERGYSFSLTT FSPSGKLVQI EYALAAVAGG APSVGIKAAN GVVLATEKKQ KSILYDERSV HKVEPITKHI GLVYSGMGPD YRVLVHRAR KLAQQYYLVY QEPIPTAQLV QRVASVMQEY TQSGGVRPFG VSLLICGWNE GRPYLFQSDP SGAYFAWKAT A MGKNYVNG ...String:
ERGYSFSLTT FSPSGKLVQI EYALAAVAGG APSVGIKAAN GVVLATEKKQ KSILYDERSV HKVEPITKHI GLVYSGMGPD YRVLVHRAR KLAQQYYLVY QEPIPTAQLV QRVASVMQEY TQSGGVRPFG VSLLICGWNE GRPYLFQSDP SGAYFAWKAT A MGKNYVNG KTFLEKRYNE DLELEDAIHT AILTLKESFE GQMTEDNIEV GICNEAGFRR LTPTEVKDYL AAIA

UniProtKB: Proteasome subunit alpha type-2

+
Macromolecule #20: Proteasome subunit alpha type-4

MacromoleculeName: Proteasome subunit alpha type-4 / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 28.118189 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SRRYDSRTTI FSPEGRLYQV EYAMEAIGHA GTCLGILAND GVLLAAERRN IHKLLDEVFF SEKIYKLNED MACSVAGITS DANVLTNEL RLIAQRYLLQ YQEPIPCEQL VTALCDIKQA YTQFGGKRPF GVSLLYIGWD KHYGFQLYQS DPSGNYGGWK A TCIGNNSA ...String:
SRRYDSRTTI FSPEGRLYQV EYAMEAIGHA GTCLGILAND GVLLAAERRN IHKLLDEVFF SEKIYKLNED MACSVAGITS DANVLTNEL RLIAQRYLLQ YQEPIPCEQL VTALCDIKQA YTQFGGKRPF GVSLLYIGWD KHYGFQLYQS DPSGNYGGWK A TCIGNNSA AAVSMLKQDY KEGEMTLKSA LALAIKVLNK TMDVSKLSAE KVEIATLTRE NGKTVIRVLK QKEVEQLIKK HE EEEAKAE REK

UniProtKB: Proteasome subunit alpha type-4

+
Macromolecule #21: Proteasome subunit alpha type-7

MacromoleculeName: Proteasome subunit alpha type-7 / type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 27.382178 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SYDRAITVFS PDGHLFQVEY AQEAVKKGST AVGVRGRDIV VLGVEKKSVA KLQDERTVRK ICALDDNVCM AFAGLTADAR IVINRARVE CQSHRLTVED PVTVEYITRY IASLKQRYTQ SNGRRPFGIS ALIVGFDFDG TPRLYQTDPS GTYHAWKANA I GRGAKSVR ...String:
SYDRAITVFS PDGHLFQVEY AQEAVKKGST AVGVRGRDIV VLGVEKKSVA KLQDERTVRK ICALDDNVCM AFAGLTADAR IVINRARVE CQSHRLTVED PVTVEYITRY IASLKQRYTQ SNGRRPFGIS ALIVGFDFDG TPRLYQTDPS GTYHAWKANA I GRGAKSVR EFLEKNYTDE AIETDDLTIK LVIKALLEVV QSGGKNIELA VMRRDQSLKI LNPEEIEKYV AEIEKEKEEN EK KKQ

UniProtKB: Proteasome subunit alpha type-7

+
Macromolecule #22: Proteasome subunit alpha type-5

MacromoleculeName: Proteasome subunit alpha type-5 / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.569957 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: YDRGVNTFSP EGRLFQVEYA IEAIKLGSTA IGIQTSEGVC LAVEKRITSP LMEPSSIEKI VEIDAHIGCA MSGLIADAKT LIDKARVET QNHWFTYNET MTVESVTQAV SNLALQFGEE DADPGAMSRP FGVALLFGGV DEKGPQLFHM DPSGTFVQCD A RAIGSASE ...String:
YDRGVNTFSP EGRLFQVEYA IEAIKLGSTA IGIQTSEGVC LAVEKRITSP LMEPSSIEKI VEIDAHIGCA MSGLIADAKT LIDKARVET QNHWFTYNET MTVESVTQAV SNLALQFGEE DADPGAMSRP FGVALLFGGV DEKGPQLFHM DPSGTFVQCD A RAIGSASE GAQSSLQEVY HKSMTLKEAI KSSLIILKQV MEEKLNATNI ELATVQPGQN FHMFTKEELE EVIKDI

UniProtKB: Proteasome subunit alpha type-5

+
Macromolecule #23: Proteasome subunit alpha type-1

MacromoleculeName: Proteasome subunit alpha type-1 / type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 26.728428 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: NQYDNDVTVW SPQGRIHQIE YAMEAVKQGS ATVGLKSKTH AVLVALKRAQ SELAAHQKKI LHVDNHIGIS IAGLTADARL LCNFMRQEC LDSRFVFDRP LPVSRLVSLI GSKTQIPTQR YGRRPYGVGL LIAGYDDMGP HIFQTCPSAN YFDCRAMSIG A RSQSARTY ...String:
NQYDNDVTVW SPQGRIHQIE YAMEAVKQGS ATVGLKSKTH AVLVALKRAQ SELAAHQKKI LHVDNHIGIS IAGLTADARL LCNFMRQEC LDSRFVFDRP LPVSRLVSLI GSKTQIPTQR YGRRPYGVGL LIAGYDDMGP HIFQTCPSAN YFDCRAMSIG A RSQSARTY LERHMSEFME CNLNELVKHG LRALRETLPA EQDLTTKNVS IGIVGKDLEF TIYDDDDVSP FLEGLEERPQ

UniProtKB: Proteasome subunit alpha type-1

+
Macromolecule #24: Proteasome subunit alpha type-3

MacromoleculeName: Proteasome subunit alpha type-3 / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 27.2871 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SSIGTGYDLS ASTFSPDGRV FQVEYAMKAV ENSSTAIGIR CKDGVVFGVE KLVLSKLYEE GSNKRLFNVD RHVGMAVAGL LADARSLAD IAREEASNFR SNFGYNIPLK HLADRVAMYV HAYTLYSAVR PFGCSFMLGS YSVNDGAQLY MIDPSGVSYG Y WGCAIGKA ...String:
SSIGTGYDLS ASTFSPDGRV FQVEYAMKAV ENSSTAIGIR CKDGVVFGVE KLVLSKLYEE GSNKRLFNVD RHVGMAVAGL LADARSLAD IAREEASNFR SNFGYNIPLK HLADRVAMYV HAYTLYSAVR PFGCSFMLGS YSVNDGAQLY MIDPSGVSYG Y WGCAIGKA RQAAKTEIEK LQMKEMTCRD IVKEVAKIIY IVHDEVKDKA FELELSWVGE LTNGRHEIVP KDIREEAEKY AK ESLKE

UniProtKB: Proteasome subunit alpha type-3

+
Macromolecule #25: Proteasome subunit beta type-6

MacromoleculeName: Proteasome subunit beta type-6 / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.471129 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
TTIMAVQFDG GVVLGADSRT TTGSYIANRV TDKLTPIHDR IFCCRSGSAA DTQAVADAVT YQLGFHSIEL NEPPLVHTAA SLFKEMCYR YREDLMAGII IAGWDPQEGG QVYSVPMGGM MVRQSFAIGG SGSSYIYGYV DATYREGMTK EECLQFTANA L ALAMERDG SSGGVIRLAA IAESGVERQV LLG

UniProtKB: Proteasome subunit beta type-6

+
Macromolecule #26: Proteasome subunit beta type-7

MacromoleculeName: Proteasome subunit beta type-7 / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.745256 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: TTIAGVVYKD GIVLGADTRA TEGMVVADKN CSKIHFISPN IYCCGAGTAA DTDMTTQLIS SNLELHSLST GRLPRVVTAN RMLKQMLFR YQGYIGAALV LGGVDVTGPH LYSIYPHGST DKLPYVTMGS GSLAAMAVFE DKFRPDMEEE EAKNLVSEAI A AGIFNDLG ...String:
TTIAGVVYKD GIVLGADTRA TEGMVVADKN CSKIHFISPN IYCCGAGTAA DTDMTTQLIS SNLELHSLST GRLPRVVTAN RMLKQMLFR YQGYIGAALV LGGVDVTGPH LYSIYPHGST DKLPYVTMGS GSLAAMAVFE DKFRPDMEEE EAKNLVSEAI A AGIFNDLG SGSNIDLCVI SKNKLDFLRP YTVPNKKGTR LGRYRCEKGT TAVLTEKITP LE

UniProtKB: Proteasome subunit beta type-7

+
Macromolecule #27: Proteasome subunit beta type-3

MacromoleculeName: Proteasome subunit beta type-3 / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.841701 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SIMSYNGGAV MAMKGKNCVA IAADRRFGIQ AQMVTTDFQK IFPMGDRLYI GLAGLATDVQ TVAQRLKFRL NLYELKEGRQ IKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP D HLFETISQ ...String:
SIMSYNGGAV MAMKGKNCVA IAADRRFGIQ AQMVTTDFQK IFPMGDRLYI GLAGLATDVQ TVAQRLKFRL NLYELKEGRQ IKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP D HLFETISQ AMLNAVDRDA VSGMGVIVHI IEKDKITTRT LKARMD

UniProtKB: Proteasome subunit beta type-3

+
Macromolecule #28: Proteasome subunit beta type-2

MacromoleculeName: Proteasome subunit beta type-2 / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.720146 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLA DCLRSRTPYH VNLLLAGYDE HEGPALYYMD YLAALAKAPF AAHGYGAFLT LSILDRYYTP TISRERAVEL L RKCLEELQ ...String:
MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLA DCLRSRTPYH VNLLLAGYDE HEGPALYYMD YLAALAKAPF AAHGYGAFLT LSILDRYYTP TISRERAVEL L RKCLEELQ KRFILNLPTF SVRIIDKNGI HDLDNISFPK Q

UniProtKB: Proteasome subunit beta type-2

+
Macromolecule #29: Proteasome subunit beta type-5

MacromoleculeName: Proteasome subunit beta type-5 / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.199072 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: TTTLAFKFRH GVIVAADSRA TAGAYIASQT VKKVIEINPY LLGTMAGGAA DCSFWERLLA RQCRIYELRN KERISVAAAS KLLANMVYQ YKGMGLSMGT MICGWDKRGP GLYYVDSEGN RISGATFSVG SGSVYAYGVM DRGYSYDLEV EQAYDLARRA I YQATYRDA ...String:
TTTLAFKFRH GVIVAADSRA TAGAYIASQT VKKVIEINPY LLGTMAGGAA DCSFWERLLA RQCRIYELRN KERISVAAAS KLLANMVYQ YKGMGLSMGT MICGWDKRGP GLYYVDSEGN RISGATFSVG SGSVYAYGVM DRGYSYDLEV EQAYDLARRA I YQATYRDA YSGGAVNLYH VREDGWIRVS SDNVADLHEK YSG

UniProtKB: Proteasome subunit beta type-5

+
Macromolecule #30: Proteasome subunit beta type-1

MacromoleculeName: Proteasome subunit beta type-1 / type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.578986 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: RFSPYVFNGG TILAIAGEDF AIVASDTRLS EGFSIHTRDS PKCYKLTDKT VIGCSGFHGD CLTLTKIIEA RLKMYKHSNN KAMTTGAIA AMLSTILYSR RFFPYYVYNI IGGLDEEGKG AVYSFDPVGS YQRDSFKAGG SASAMLQPLL DNQVGFKNMQ N VEHVPLSL ...String:
RFSPYVFNGG TILAIAGEDF AIVASDTRLS EGFSIHTRDS PKCYKLTDKT VIGCSGFHGD CLTLTKIIEA RLKMYKHSNN KAMTTGAIA AMLSTILYSR RFFPYYVYNI IGGLDEEGKG AVYSFDPVGS YQRDSFKAGG SASAMLQPLL DNQVGFKNMQ N VEHVPLSL DRAMRLVKDV FISAAERDVY TGDALRICIV TKEGIREETV SLRKD

UniProtKB: Proteasome subunit beta type-1

+
Macromolecule #31: Proteasome subunit beta type-4

MacromoleculeName: Proteasome subunit beta type-4 / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO / EC number: proteasome endopeptidase complex
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.994324 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: TQNPMVTGTS VLGVKFEGGV VIAADMLGSY GSLARFRNIS RIMRVNNSTM LGASGDYADF QYLKQVLGQM VIDEELLGDG HSYSPRAIH SWLTRAMYSR RSKMNPLWNT MVIGGYADGE SFLGYVDMLG VAYEAPSLAT GYGAYLAQPL LREVLEKQPV L SQTEARDL ...String:
TQNPMVTGTS VLGVKFEGGV VIAADMLGSY GSLARFRNIS RIMRVNNSTM LGASGDYADF QYLKQVLGQM VIDEELLGDG HSYSPRAIH SWLTRAMYSR RSKMNPLWNT MVIGGYADGE SFLGYVDMLG VAYEAPSLAT GYGAYLAQPL LREVLEKQPV L SQTEARDL VERCMRVLYY RDARSYNRFQ IATVTEKGVE IEGPLSTETN WDIAHMI

UniProtKB: Proteasome subunit beta type-4

+
Macromolecule #32: 26S proteasome non-ATPase regulatory subunit 2

MacromoleculeName: 26S proteasome non-ATPase regulatory subunit 2 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 100.313625 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MEEGGRDKAP VQPQQSPAAA PGGTDEKPSG KERRDAGDKD KEQELSEEDK QLQDELEMLV ERLGEKDTSL YRPALEELRR QIRSSTTSM TSVPKPLKFL RPHYGKLKEI YENMAPGENK RFAADIISVL AMTMSGEREC LKYRLVGSQE ELASWGHEYV R HLAGEVAK ...String:
MEEGGRDKAP VQPQQSPAAA PGGTDEKPSG KERRDAGDKD KEQELSEEDK QLQDELEMLV ERLGEKDTSL YRPALEELRR QIRSSTTSM TSVPKPLKFL RPHYGKLKEI YENMAPGENK RFAADIISVL AMTMSGEREC LKYRLVGSQE ELASWGHEYV R HLAGEVAK EWQELDDAEK VQREPLLTLV KEIVPYNMAH NAEHEACDLL MEIEQVDMLE KDIDENAYAK VCLYLTSCVN YV PEPENSA LLRCALGVFR KFSRFPEALR LALMLNDMEL VEDIFTSCKD VVVQKQMAFM LGRHGVFLEL SEDVEEYEDL TEI MSNVQL NSNFLALARE LDIMEPKVPD DIYKTHLENN RFGGSGSQVD SARMNLASSF VNGFVNAAFG QDKLLTDDGN KWLY KNKDH GMLSAAASLG MILLWDVDGG LTQIDKYLYS SEDYIKSGAL LACGIVNSGV RNECDPALAL LSDYVLHNSN TMRLG SIFG LGLAYAGSNR EDVLTLLLPV MGDSKSSMEV AGVTALACGM IAVGSCNGDV TSTILQTIME KSETELKDTY ARWLPL GLG LNHLGKGEAI EAILAALEVV SEPFRSFANT LVDVCAYAGS GNVLKVQQLL HICSEHFDSK EKEEDKDKKE KKDKDKK EA PADMGAHQGV AVLGIALIAM GEEIGAEMAL RTFGHLLRYG EPTLRRAVPL ALALISVSNP RLNILDTLSK FSHDADPE V SYNSIFAMGM VGSGTNNARL AAMLRQLAQY HAKDPNNLFM VRLAQGLTHL GKGTLTLCPY HSDRQLMSQV AVAGLLTVL VSFLDVRNII LGKSHYVLYG LVAAMQPRML VTFDEELRPL PVSVRVGQAV DVVGQAGKPK TITGFQTHTT PVLLAHGERA ELATEEFLP VTPILEGFVI LRKNPNYDL

UniProtKB: 26S proteasome non-ATPase regulatory subunit 2

+
Macromolecule #33: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 33 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TECNAI ARCTICA
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 30.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

-
Image processing

Startup modelType of model: OTHER
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 5.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 23567
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more