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基本情報
登録情報 | データベース: EMDB / ID: EMD-5891 | |||||||||
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タイトル | Cryo-EM structure of MAVSdeltaProTM filament | |||||||||
![]() | Cryo-EM map of filaments formed by a truncated MAVS lacking part of the proline-rich region and the C-terminal transmembrane domain | |||||||||
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![]() | Innate immunity / helical filament / cryoEM / helical reconstruction | |||||||||
機能・相同性 | ![]() positive regulation of IP-10 production / regulation of peroxisome organization / RIG-I binding / positive regulation of chemokine (C-C motif) ligand 5 production / positive regulation of myeloid dendritic cell cytokine production / CARD domain binding / NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 / protein localization to mitochondrion / positive regulation of response to cytokine stimulus / positive regulation of type I interferon-mediated signaling pathway ...positive regulation of IP-10 production / regulation of peroxisome organization / RIG-I binding / positive regulation of chemokine (C-C motif) ligand 5 production / positive regulation of myeloid dendritic cell cytokine production / CARD domain binding / NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 / protein localization to mitochondrion / positive regulation of response to cytokine stimulus / positive regulation of type I interferon-mediated signaling pathway / peroxisomal membrane / TRAF6 mediated IRF7 activation / negative regulation of type I interferon-mediated signaling pathway / positive regulation of NLRP3 inflammasome complex assembly / cytoplasmic pattern recognition receptor signaling pathway / negative regulation of viral genome replication / type I interferon-mediated signaling pathway / cellular response to exogenous dsRNA / TRAF6 mediated NF-kB activation / positive regulation of interferon-alpha production / positive regulation of type I interferon production / signaling adaptor activity / ubiquitin ligase complex / antiviral innate immune response / positive regulation of defense response to virus by host / activation of innate immune response / positive regulation of interferon-beta production / molecular condensate scaffold activity / positive regulation of interleukin-8 production / Negative regulators of DDX58/IFIH1 signaling / positive regulation of DNA-binding transcription factor activity / DDX58/IFIH1-mediated induction of interferon-alpha/beta / PKR-mediated signaling / mitochondrial membrane / Evasion by RSV of host interferon responses / positive regulation of protein import into nucleus / positive regulation of interleukin-6 production / SARS-CoV-1 activates/modulates innate immune responses / positive regulation of tumor necrosis factor production / Ovarian tumor domain proteases / TRAF3-dependent IRF activation pathway / positive regulation of canonical NF-kappaB signal transduction / defense response to virus / mitochondrial outer membrane / molecular adaptor activity / defense response to bacterium / positive regulation of protein phosphorylation / innate immune response / protein kinase binding / SARS-CoV-2 activates/modulates innate and adaptive immune responses / signal transduction / positive regulation of transcription by RNA polymerase II / mitochondrion / identical protein binding 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | らせん対称体再構成法 / クライオ電子顕微鏡法 / 解像度: 16.4 Å | |||||||||
![]() | Xu H / He X / Zheng H / Huang LJ / Hou F / Yu Z / de la Cruz MJ / Borkowski B / Zhang X / Chen ZJ / Jiang Q-X | |||||||||
![]() | ![]() タイトル: Structural basis for the prion-like MAVS filaments in antiviral innate immunity. 著者: Hui Xu / Xiaojing He / Hui Zheng / Lily J Huang / Fajian Hou / Zhiheng Yu / Michael Jason de la Cruz / Brian Borkowski / Xuewu Zhang / Zhijian J Chen / Qiu-Xing Jiang / ![]() 要旨: Mitochondrial antiviral signaling (MAVS) protein is required for innate immune responses against RNA viruses. In virus-infected cells MAVS forms prion-like aggregates to activate antiviral signaling ...Mitochondrial antiviral signaling (MAVS) protein is required for innate immune responses against RNA viruses. In virus-infected cells MAVS forms prion-like aggregates to activate antiviral signaling cascades, but the underlying structural mechanism is unknown. Here we report cryo-electron microscopic structures of the helical filaments formed by both the N-terminal caspase activation and recruitment domain (CARD) of MAVS and a truncated MAVS lacking part of the proline-rich region and the C-terminal transmembrane domain. Both structures are left-handed three-stranded helical filaments, revealing specific interfaces between individual CARD subunits that are dictated by electrostatic interactions between neighboring strands and hydrophobic interactions within each strand. Point mutations at multiple locations of these two interfaces impaired filament formation and antiviral signaling. Super-resolution imaging of virus-infected cells revealed rod-shaped MAVS clusters on mitochondria. These results elucidate the structural mechanism of MAVS polymerization, and explain how an α-helical domain uses distinct chemical interactions to form self-perpetuating filaments. DOI: http://dx.doi.org/10.7554/eLife.01489.001. | |||||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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ダウンロードとリンク
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マップデータ | ![]() | 1 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 11.2 KB 11.2 KB | 表示 表示 | ![]() |
画像 | ![]() | 144.8 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 5890C ![]() 3j6cC ![]() 4o9fC ![]() 4o9lC C: 同じ文献を引用 ( |
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類似構造データ | |
電子顕微鏡画像生データ | ![]() Data #5: CARD set1 segments [picked particles - multiframe - unprocessed] Data #6: CARD set2 segments [picked particles - multiframe - unprocessed] Data #7: CARD set3 segments [picked particles - multiframe - unprocessed] Data #8: CARD set4 segments [picked particles - multiframe - unprocessed] Data #9: DeltProTM micrographs [micrographs - single frame] Data #10: DeltaProTM segments [picked particles - multiframe - unprocessed]) |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Cryo-EM map of filaments formed by a truncated MAVS lacking part of the proline-rich region and the C-terminal transmembrane domain | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 2.333 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
-全体 : Filaments formed by a truncated MAVS lacking part of the proline-...
全体 | 名称: Filaments formed by a truncated MAVS lacking part of the proline-rich region and the C-terminal transmembrane domain |
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要素 |
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-超分子 #1000: Filaments formed by a truncated MAVS lacking part of the proline-...
超分子 | 名称: Filaments formed by a truncated MAVS lacking part of the proline-rich region and the C-terminal transmembrane domain タイプ: sample / ID: 1000 / 集合状態: polymer / Number unique components: 1 |
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分子量 | 理論値: 1.396 MDa |
-分子 #1: Mitochondrial antiviral-signaling protein (MAVS)
分子 | 名称: Mitochondrial antiviral-signaling protein (MAVS) / タイプ: protein_or_peptide / ID: 1 / Name.synonym: IPS1, KIAA1271, VISA / 集合状態: polymer / 組換発現: Yes |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 42.3 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | UniProtKB: Mitochondrial antiviral-signaling protein |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | らせん対称体再構成法 |
試料の集合状態 | filament |
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試料調製
緩衝液 | pH: 8 / 詳細: 10mM Tris-HCl, 150mM NaCl, 1mM DTT |
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グリッド | 詳細: Quantifoil grids coated with thin carbon on top |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / 装置: FEI VITROBOT MARK III |
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電子顕微鏡法
顕微鏡 | JEOL 2200FS |
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アライメント法 | Legacy - 非点収差: Objective lens astigmatism was corrected at 100,000 x magnification |
特殊光学系 | エネルギーフィルター - 名称: JEOL omega filter エネルギーフィルター - エネルギー下限: 0.0 eV エネルギーフィルター - エネルギー上限: 35.0 eV |
日付 | 2011年11月30日 |
撮影 | カテゴリ: FILM / フィルム・検出器のモデル: KODAK SO-163 FILM / デジタル化 - スキャナー: ZEISS SCAI / デジタル化 - サンプリング間隔: 7 µm / 平均電子線量: 20 e/Å2 |
電子線 | 加速電圧: 200 kV / 電子線源: ![]() |
電子光学系 | 倍率(補正後): 61950 / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.0 mm / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 0.8 µm / 倍率(公称値): 60000 |
試料ステージ | 試料ホルダーモデル: GATAN LIQUID NITROGEN |
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画像解析
詳細 | IHRSR. Due to the smaller size of the dataset, we did not sort the data based on variations in symmetry parameters. |
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最終 再構成 | 想定した対称性 - らせんパラメータ - Δz: 16.9 Å 想定した対称性 - らせんパラメータ - ΔΦ: 52.9 ° 想定した対称性 - らせんパラメータ - 軸対称性: C3 (3回回転対称) アルゴリズム: OTHER / 解像度のタイプ: BY AUTHOR / 解像度: 16.4 Å / 解像度の算出法: OTHER / ソフトウェア - 名称: Spider, MRC, EMAN, IMAGIC4D |
CTF補正 | 詳細: Each filament segment |