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Open data
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Basic information
Entry | Database: EMDB / ID: 6663 |
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Title | Structure of RIP2 CARD domain |
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![]() | Active RIP2 signaling complex
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Source | ![]() ![]() |
Method | helical reconstruction / ![]() |
![]() | Wu B / Gong Q |
![]() | Journal: Nat Commun / Year: 2018 Title: Structural basis of RIP2 activation and signaling. ![]() |
Date | Deposition: Oct 31, 2016 / Header (metadata) release: May 23, 2018 / Map release: May 23, 2018 / Last update: May 23, 2018 |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
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Download
![]() | ![]() |
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Header (meta data in XML format) | ![]() ![]() ![]() |
Images | ![]() |
Others | ![]() ![]() |
FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-6663 |
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Links
-Related structure data
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.31 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire Active RIP2 signaling complex
Entire | Name: Active RIP2 signaling complex / Number of components: 2 |
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-Component #1: cellular-component, Active RIP2 signaling complex
Cellular-component | Name: Active RIP2 signaling complex / Recombinant expression: No |
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Source | Species: ![]() ![]() |
Source (engineered) | Expression System: ![]() ![]() ![]() |
-Component #2: protein, Receptor-interacting serine/threonine-protein kinase 2
Protein | Name: Receptor-interacting serine/threonine-protein kinase 2 Number of Copies: 12 / Recombinant expression: No |
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Mass | Theoretical: 10.751217 kDa |
Source | Species: ![]() ![]() |
Source (engineered) | Expression System: ![]() ![]() ![]() |
-Experimental details
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Sample preparation
Specimen | Specimen state: filament / Method: ![]() |
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Helical parameters | Axial symmetry: C1 (asymmetric) / Delta z: 4.9448 Å / Delta phi: -101.188 deg. |
Sample solution | Specimen conc.: 0.2 mg/ml / pH: 7.4 |
Vitrification | Cryogen name: METHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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![]() | Microscope: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN![]() |
Lens | Imaging mode: BRIGHT FIELD![]() |
Specimen Holder | Model: FEI TITAN KRIOS AUTOGRID HOLDER |
Camera | Detector: FEI FALCON II (4k x 4k) |
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Image processing
![]() | Method: helical reconstruction |
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3D reconstruction | Software: RELION / Resolution: 4.85 Å / Resolution method: FSC 0.143 CUT-OFF Details: The model is only a partial model. Indefinite filament model could be produced by a helical rotation. |
-Atomic model buiding
Modeling #1 | Refinement protocol: flexible / Refinement space: REAL |
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Output model |