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Yorodumi- EMDB-41100: CCW Flagellar Switch Complex - FliF, FliG, FliM, and FliN forming... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-41100 | |||||||||
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Title | CCW Flagellar Switch Complex - FliF, FliG, FliM, and FliN forming 34-mer C-ring from Salmonella | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Domain Swap / Symmetry mismatch / Flagellar component / Switch complex / MOTOR PROTEIN | |||||||||
Function / homology | Function and homology information bacterial-type flagellum basal body, MS ring / bacterial-type flagellum basal body / bacterial-type flagellum-dependent swarming motility / positive chemotaxis / cytoskeletal motor activity / bacterial-type flagellum-dependent cell motility / chemotaxis / plasma membrane Similarity search - Function | |||||||||
Biological species | Salmonella enterica subsp. enterica serovar Typhimurium (bacteria) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.0 Å | |||||||||
Authors | Singh PK / Iverson TM | |||||||||
Funding support | United States, 1 items
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Citation | Journal: To Be Published Title: Structural basis for directional rotation of the Salmonella flagellum Authors: Singh PK / Iverson TM | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_41100.map.gz | 860.9 MB | EMDB map data format | |
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Header (meta data) | emd-41100-v30.xml emd-41100.xml | 18.6 KB 18.6 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_41100_fsc.xml | 28.6 KB | Display | FSC data file |
Images | emd_41100.png | 69 KB | ||
Masks | emd_41100_msk_1.map | 1.7 GB | Mask map | |
Filedesc metadata | emd-41100.cif.gz | 5.9 KB | ||
Others | emd_41100_half_map_1.map.gz emd_41100_half_map_2.map.gz | 1.6 GB 1.6 GB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-41100 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-41100 | HTTPS FTP |
-Validation report
Summary document | emd_41100_validation.pdf.gz | 873.3 KB | Display | EMDB validaton report |
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Full document | emd_41100_full_validation.pdf.gz | 872.9 KB | Display | |
Data in XML | emd_41100_validation.xml.gz | 34.3 KB | Display | |
Data in CIF | emd_41100_validation.cif.gz | 46.9 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-41100 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-41100 | HTTPS FTP |
-Related structure data
Related structure data | 8t8oMC 8t8pC C: citing same article (ref.) M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_41100.map.gz / Format: CCP4 / Size: 1.7 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.363 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_41100_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_41100_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_41100_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Flagellar MS-ring and C-ring complex containing FliF, FliG, FliM,...
Entire | Name: Flagellar MS-ring and C-ring complex containing FliF, FliG, FliM, and FliN |
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Components |
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-Supramolecule #1: Flagellar MS-ring and C-ring complex containing FliF, FliG, FliM,...
Supramolecule | Name: Flagellar MS-ring and C-ring complex containing FliF, FliG, FliM, and FliN type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Salmonella enterica subsp. enterica serovar Typhimurium (bacteria) |
Molecular weight | Theoretical: 3.5 MDa |
-Macromolecule #1: Flagellar M-ring protein
Macromolecule | Name: Flagellar M-ring protein / type: protein_or_peptide / ID: 1 / Number of copies: 34 / Enantiomer: LEVO |
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Source (natural) | Organism: Salmonella enterica subsp. enterica serovar Typhimurium (bacteria) |
Molecular weight | Theoretical: 5.644328 KDa |
Recombinant expression | Organism: Escherichia coli BL21(DE3) (bacteria) |
Sequence | String: KDEQLQQRRA NQRLGAEVMS QRIREMSDND PRVVALVIRQ WMSNDHE UniProtKB: Flagellar M-ring protein |
-Macromolecule #2: Flagellar motor switch protein FliG
Macromolecule | Name: Flagellar motor switch protein FliG / type: protein_or_peptide / ID: 2 / Number of copies: 34 / Enantiomer: LEVO |
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Source (natural) | Organism: Salmonella enterica subsp. enterica serovar Typhimurium (bacteria) |
Molecular weight | Theoretical: 36.890957 KDa |
Recombinant expression | Organism: Escherichia coli BL21(DE3) (bacteria) |
Sequence | String: MSNLSGTDKS VILLMTIGED RAAEVFKHLS TREVQALSTA MANVRQISNK QLTDVLSEFE QEAEQFAALN INANEYLRSV LVKALGEER ASSLLEDILE TRDTTSGIET LNFMEPQSAA DLIRDEHPQI IATILVHLKR SQAADILALF DERLRHDVML R IATFGGVQ ...String: MSNLSGTDKS VILLMTIGED RAAEVFKHLS TREVQALSTA MANVRQISNK QLTDVLSEFE QEAEQFAALN INANEYLRSV LVKALGEER ASSLLEDILE TRDTTSGIET LNFMEPQSAA DLIRDEHPQI IATILVHLKR SQAADILALF DERLRHDVML R IATFGGVQ PAALAELTEV LNGLLDGQNL KRSKMGGVRT AAEIINLMKT QQEEAVITAV REFDGELAQK IIDEMFLFEN LV DVDDRSI QRLLQEVDSE SLLIALKGAE PPLREKFLRN MSQRAADILR DDLANRGPVR LSQVENEQKA ILLIVRRLAE TGE MVIGSG EDTYV UniProtKB: Flagellar motor switch protein FliG |
-Macromolecule #3: FliM,Flagellar motor switch protein FliM
Macromolecule | Name: FliM,Flagellar motor switch protein FliM / type: protein_or_peptide / ID: 3 / Number of copies: 34 / Enantiomer: LEVO |
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Source (natural) | Organism: Salmonella enterica subsp. enterica serovar Typhimurium (bacteria) |
Molecular weight | Theoretical: 33.758836 KDa |
Recombinant expression | Organism: Escherichia coli BL21(DE3) (bacteria) |
Sequence | String: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) LQA LEIINERFAR QFRMGLFNLL RRSPDITVGA IRIQPYHEFA RNLPVPTNLN LIHLKPLRGT GLVVFSPSLV FIAVDNL FG GDGRFPTKVE ...String: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) LQA LEIINERFAR QFRMGLFNLL RRSPDITVGA IRIQPYHEFA RNLPVPTNLN LIHLKPLRGT GLVVFSPSLV FIAVDNL FG GDGRFPTKVE GREFTHTEQR VINRMLKLAL EGYSDAWKAI NPLEVEYVRS EMQVKFTNIT TSPNDIVVNT PFHVEIGN L TGEFNICLPF SMIEPLRELL VNPPLENSRH EDQNWRDNLV RQVQHSELEL VANFADIPLR LSQILKLKPG DVLPIEKPD RIIAHVDGVP VLTSQYGTVN GQYALRVEHL INPILNSLNE EQPK UniProtKB: Flagellar motor switch protein FliM |
-Macromolecule #4: Flagellar motor switch protein FliN
Macromolecule | Name: Flagellar motor switch protein FliN / type: protein_or_peptide / ID: 4 / Number of copies: 102 / Enantiomer: LEVO |
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Source (natural) | Organism: Salmonella enterica subsp. enterica serovar Typhimurium (bacteria) |
Molecular weight | Theoretical: 14.801823 KDa |
Recombinant expression | Organism: Escherichia coli BL21(DE3) (bacteria) |
Sequence | String: MSDMNNPSDE NTGALDDLWA DALNEQKATT TKSAADAVFQ QLGGGDVSGA MQDIDLIMDI PVKLTVELGR TRMTIKELLR LTQGSVVAL DGLAGEPLDI LINGYLIAQG EVVVVADKYG VRITDIITPS ERMRRLSR UniProtKB: Flagellar motor switch protein FliN |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 51.557 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |