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- PDB-8t8o: CCW Flagellar Switch Complex - FliF, FliG, FliM, and FliN forming... -

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Basic information

Entry
Database: PDB / ID: 8t8o
TitleCCW Flagellar Switch Complex - FliF, FliG, FliM, and FliN forming 34-mer C-ring from Salmonella
Components
  • Flagellar M-ring protein
  • Flagellar motor switch protein FliGFlagellar motor switch protein
  • Flagellar motor switch protein FliNFlagellar motor switch protein
  • FliM,Flagellar motor switch protein FliM
KeywordsMOTOR PROTEIN / Domain Swap / Symmetry mismatch / Flagellar component / Switch complex
Function / homology
Function and homology information


bacterial-type flagellum basal body, MS ring / bacterial-type flagellum basal body / cytoskeletal motor activity / bacterial-type flagellum-dependent cell motility / chemotaxis / plasma membrane
Similarity search - Function
Flagellar motor switch protein FliN, N-terminal / Flagellar motor switch protein FliN N-terminal / Flagellar motor switch FliN / Flagellar motor switch FliN/Type III secretion HrcQb / Flagellar motor switch protein FliG / Flagellar motor switch protein FliM / Flagellar motor switch protein FliG, alpha-helical / Flagellar motor switch protein FliG, C-terminal / Flagellar motor switch protein FliG, N-terminal domain / Flagellar motor switch protein FliG, middle domain ...Flagellar motor switch protein FliN, N-terminal / Flagellar motor switch protein FliN N-terminal / Flagellar motor switch FliN / Flagellar motor switch FliN/Type III secretion HrcQb / Flagellar motor switch protein FliG / Flagellar motor switch protein FliM / Flagellar motor switch protein FliG, alpha-helical / Flagellar motor switch protein FliG, C-terminal / Flagellar motor switch protein FliG, N-terminal domain / Flagellar motor switch protein FliG, middle domain / FliG C-terminal domain / Flagellar motor switch protein FliM / FliG middle domain / FliG N-terminal domain / Flagellar motor switch protein FliN-like, C-terminal domain / SpoA-like superfamily / Type III flagellar switch regulator (C-ring) FliN C-term / CheC-like superfamily / Flagellar M-ring protein FliF / Flagellar M-ring C-terminal / Flagellar M-ring protein C-terminal / Lipoprotein YscJ/Flagellar M-ring protein / Secretory protein of YscJ/FliF family / Flagellar M-ring , N-terminal / AMP-binding enzyme, C-terminal domain superfamily
Similarity search - Domain/homology
Flagellar motor switch protein FliM / Flagellar motor switch protein FliN / Flagellar motor switch protein FliG / Flagellar M-ring protein
Similarity search - Component
Biological speciesSalmonella enterica subsp. enterica serovar Typhimurium (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4 Å
AuthorsSingh, P.K. / Iverson, T.M.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/John E. Fogarty International Center (NIH/FIC)GM61606 United States
CitationJournal: To Be Published
Title: Structural basis for directional rotation of the Salmonella flagellum
Authors: Singh, P.K. / Iverson, T.M.
History
DepositionJun 22, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 28, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
F: Flagellar M-ring protein
G: Flagellar motor switch protein FliG
M: FliM,Flagellar motor switch protein FliM
N: Flagellar motor switch protein FliN
P: Flagellar motor switch protein FliN
Q: Flagellar motor switch protein FliN
A: Flagellar M-ring protein
B: Flagellar motor switch protein FliG
C: FliM,Flagellar motor switch protein FliM
D: Flagellar motor switch protein FliN
E: Flagellar motor switch protein FliN
H: Flagellar motor switch protein FliN
I: Flagellar M-ring protein
J: Flagellar motor switch protein FliG
K: FliM,Flagellar motor switch protein FliM
L: Flagellar motor switch protein FliN
O: Flagellar motor switch protein FliN
R: Flagellar motor switch protein FliN
S: Flagellar M-ring protein
T: Flagellar motor switch protein FliG
V: FliM,Flagellar motor switch protein FliM
W: Flagellar motor switch protein FliN
X: Flagellar motor switch protein FliN
Y: Flagellar motor switch protein FliN
Z: Flagellar M-ring protein
AA: Flagellar motor switch protein FliG
BA: FliM,Flagellar motor switch protein FliM
CA: Flagellar motor switch protein FliN
DA: Flagellar motor switch protein FliN
EA: Flagellar motor switch protein FliN
FA: Flagellar M-ring protein
GA: Flagellar motor switch protein FliG
HA: FliM,Flagellar motor switch protein FliM
IA: Flagellar motor switch protein FliN
JA: Flagellar motor switch protein FliN
KA: Flagellar motor switch protein FliN
LA: Flagellar M-ring protein
MA: Flagellar motor switch protein FliG
NA: FliM,Flagellar motor switch protein FliM
OA: Flagellar motor switch protein FliN
PA: Flagellar motor switch protein FliN
QA: Flagellar motor switch protein FliN
RA: Flagellar M-ring protein
SA: Flagellar motor switch protein FliG
TA: FliM,Flagellar motor switch protein FliM
UA: Flagellar motor switch protein FliN
VA: Flagellar motor switch protein FliN
WA: Flagellar motor switch protein FliN
XA: Flagellar M-ring protein
YA: Flagellar motor switch protein FliG
ZA: FliM,Flagellar motor switch protein FliM
AB: Flagellar motor switch protein FliN
BB: Flagellar motor switch protein FliN
CB: Flagellar motor switch protein FliN
DB: Flagellar M-ring protein
EB: Flagellar motor switch protein FliG
FB: FliM,Flagellar motor switch protein FliM
GB: Flagellar motor switch protein FliN
HB: Flagellar motor switch protein FliN
IB: Flagellar motor switch protein FliN
JB: Flagellar M-ring protein
KB: Flagellar motor switch protein FliG
LB: FliM,Flagellar motor switch protein FliM
MB: Flagellar motor switch protein FliN
NB: Flagellar motor switch protein FliN
OB: Flagellar motor switch protein FliN
PB: Flagellar M-ring protein
QB: Flagellar motor switch protein FliG
RB: FliM,Flagellar motor switch protein FliM
SB: Flagellar motor switch protein FliN
TB: Flagellar motor switch protein FliN
UB: Flagellar motor switch protein FliN
VB: Flagellar M-ring protein
WB: Flagellar motor switch protein FliG
XB: FliM,Flagellar motor switch protein FliM
YB: Flagellar motor switch protein FliN
ZB: Flagellar motor switch protein FliN
AC: Flagellar motor switch protein FliN
BC: Flagellar M-ring protein
CC: Flagellar motor switch protein FliG
DC: FliM,Flagellar motor switch protein FliM
EC: Flagellar motor switch protein FliN
FC: Flagellar motor switch protein FliN
GC: Flagellar motor switch protein FliN
HC: Flagellar M-ring protein
IC: Flagellar motor switch protein FliG
JC: FliM,Flagellar motor switch protein FliM
KC: Flagellar motor switch protein FliN
LC: Flagellar motor switch protein FliN
MC: Flagellar motor switch protein FliN
NC: Flagellar M-ring protein
OC: Flagellar motor switch protein FliG
PC: FliM,Flagellar motor switch protein FliM
QC: Flagellar motor switch protein FliN
RC: Flagellar motor switch protein FliN
SC: Flagellar motor switch protein FliN
TC: Flagellar M-ring protein
UC: Flagellar motor switch protein FliG
VC: FliM,Flagellar motor switch protein FliM
WC: Flagellar motor switch protein FliN
XC: Flagellar motor switch protein FliN
YC: Flagellar motor switch protein FliN
ZC: Flagellar M-ring protein
AD: Flagellar motor switch protein FliG
BD: FliM,Flagellar motor switch protein FliM
CD: Flagellar motor switch protein FliN
DD: Flagellar motor switch protein FliN
ED: Flagellar motor switch protein FliN
FD: Flagellar M-ring protein
GD: Flagellar motor switch protein FliG
HD: FliM,Flagellar motor switch protein FliM
ID: Flagellar motor switch protein FliN
JD: Flagellar motor switch protein FliN
KD: Flagellar motor switch protein FliN
LD: Flagellar M-ring protein
MD: Flagellar motor switch protein FliG
ND: FliM,Flagellar motor switch protein FliM
OD: Flagellar motor switch protein FliN
PD: Flagellar motor switch protein FliN
QD: Flagellar motor switch protein FliN
RD: Flagellar M-ring protein
SD: Flagellar motor switch protein FliG
TD: FliM,Flagellar motor switch protein FliM
UD: Flagellar motor switch protein FliN
VD: Flagellar motor switch protein FliN
WD: Flagellar motor switch protein FliN
XD: Flagellar M-ring protein
YD: Flagellar motor switch protein FliG
ZD: FliM,Flagellar motor switch protein FliM
AE: Flagellar motor switch protein FliN
BE: Flagellar motor switch protein FliN
CE: Flagellar motor switch protein FliN
DE: Flagellar M-ring protein
EE: Flagellar motor switch protein FliG
FE: FliM,Flagellar motor switch protein FliM
GE: Flagellar motor switch protein FliN
HE: Flagellar motor switch protein FliN
IE: Flagellar motor switch protein FliN
JE: Flagellar M-ring protein
KE: Flagellar motor switch protein FliG
LE: FliM,Flagellar motor switch protein FliM
ME: Flagellar motor switch protein FliN
NE: Flagellar motor switch protein FliN
OE: Flagellar motor switch protein FliN
PE: Flagellar M-ring protein
QE: Flagellar motor switch protein FliG
RE: FliM,Flagellar motor switch protein FliM
SE: Flagellar motor switch protein FliN
TE: Flagellar motor switch protein FliN
UE: Flagellar motor switch protein FliN
VE: Flagellar M-ring protein
WE: Flagellar motor switch protein FliG
XE: FliM,Flagellar motor switch protein FliM
YE: Flagellar motor switch protein FliN
ZE: Flagellar motor switch protein FliN
AF: Flagellar motor switch protein FliN
BF: Flagellar M-ring protein
CF: Flagellar motor switch protein FliG
DF: FliM,Flagellar motor switch protein FliM
EF: Flagellar motor switch protein FliN
FF: Flagellar motor switch protein FliN
GF: Flagellar motor switch protein FliN
HF: Flagellar M-ring protein
IF: Flagellar motor switch protein FliG
JF: FliM,Flagellar motor switch protein FliM
KF: Flagellar motor switch protein FliN
LF: Flagellar motor switch protein FliN
MF: Flagellar motor switch protein FliN
NF: Flagellar M-ring protein
OF: Flagellar motor switch protein FliG
PF: FliM,Flagellar motor switch protein FliM
QF: Flagellar motor switch protein FliN
RF: Flagellar motor switch protein FliN
SF: Flagellar motor switch protein FliN
TF: Flagellar M-ring protein
UF: Flagellar motor switch protein FliG
VF: FliM,Flagellar motor switch protein FliM
WF: Flagellar motor switch protein FliN
XF: Flagellar motor switch protein FliN
YF: Flagellar motor switch protein FliN
ZF: Flagellar M-ring protein
AG: Flagellar motor switch protein FliG
BG: FliM,Flagellar motor switch protein FliM
CG: Flagellar motor switch protein FliN
DG: Flagellar motor switch protein FliN
EG: Flagellar motor switch protein FliN
FG: Flagellar M-ring protein
GG: Flagellar motor switch protein FliG
HG: FliM,Flagellar motor switch protein FliM
IG: Flagellar motor switch protein FliN
JG: Flagellar motor switch protein FliN
KG: Flagellar motor switch protein FliN
LG: Flagellar M-ring protein
MG: Flagellar motor switch protein FliG
NG: FliM,Flagellar motor switch protein FliM
OG: Flagellar motor switch protein FliN
PG: Flagellar motor switch protein FliN
QG: Flagellar motor switch protein FliN
RG: Flagellar M-ring protein
SG: Flagellar motor switch protein FliG
TG: FliM,Flagellar motor switch protein FliM
UG: Flagellar motor switch protein FliN
VG: Flagellar motor switch protein FliN
WG: Flagellar motor switch protein FliN


Theoretical massNumber of molelcules
Total (without water)4,103,786204
Polymers4,103,786204
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein/peptide ...
Flagellar M-ring protein


Mass: 5644.328 Da / Num. of mol.: 34 / Fragment: C-terminal domain (UNP residues 514-560)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Salmonella enterica subsp. enterica serovar Typhimurium (bacteria)
Gene: fliF, fla AII.1, fla BI, STM1969 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P15928
#2: Protein ...
Flagellar motor switch protein FliG / Flagellar motor switch protein


Mass: 36890.957 Da / Num. of mol.: 34
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Salmonella enterica subsp. enterica serovar Typhimurium (bacteria)
Gene: fliG / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A0F7J9E2
#3: Protein ...
FliM,Flagellar motor switch protein FliM


Mass: 33758.836 Da / Num. of mol.: 34
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Salmonella enterica subsp. enterica serovar Typhimurium (bacteria)
Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A0D6FLG5
#4: Protein ...
Flagellar motor switch protein FliN / Flagellar motor switch protein


Mass: 14801.823 Da / Num. of mol.: 102
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Salmonella enterica subsp. enterica serovar Typhimurium (bacteria)
Gene: fliN / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A0D6FLI0

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Flagellar MS-ring and C-ring complex containing FliF, FliG, FliM, and FliN
Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Molecular weightValue: 3.5 MDa / Experimental value: NO
Source (natural)Organism: Salmonella enterica subsp. enterica serovar Typhimurium (bacteria)
Source (recombinant)Organism: Escherichia coli BL21(DE3) (bacteria)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm
Image recordingElectron dose: 51.557 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

EM software
IDNameVersionCategory
4cryoSPARC4.2.1CTF correction
10cryoSPARC4.2.1initial Euler assignment
11cryoSPARC4.2.1final Euler assignment
13cryoSPARC4.2.13D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C34 (34 fold cyclic)
3D reconstructionResolution: 4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 51268 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.007206822
ELECTRON MICROSCOPYf_angle_d0.938282098
ELECTRON MICROSCOPYf_dihedral_angle_d5.71232300
ELECTRON MICROSCOPYf_chiral_restr0.05134748
ELECTRON MICROSCOPYf_plane_restr0.00738454

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