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- EMDB-22448: Plant Mitochondrial complex SC III2+IV from Vigna radiata composi... -

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Basic information

Entry
Database: EMDB / ID: EMD-22448
TitlePlant Mitochondrial complex SC III2+IV from Vigna radiata composite map
Map dataPlant mitochondrial supercomplex III2 IV from Vigna radiata. Composite map.
Sample
  • Complex: Mitochondrial Respiratory Complex III2
    • Protein or peptide: x 20 types
  • Ligand: x 11 types
Function / homology
Function and homology information


respiratory chain complex IV / mitochondrial respiratory chain complex III / mitochondrial respiratory chain complex IV / mitochondrial respirasome / quinol-cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / mitochondrial electron transport, cytochrome c to oxygen / cytochrome-c oxidase activity / mitochondrial electron transport, ubiquinol to cytochrome c / respirasome ...respiratory chain complex IV / mitochondrial respiratory chain complex III / mitochondrial respiratory chain complex IV / mitochondrial respirasome / quinol-cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / mitochondrial electron transport, cytochrome c to oxygen / cytochrome-c oxidase activity / mitochondrial electron transport, ubiquinol to cytochrome c / respirasome / 2 iron, 2 sulfur cluster binding / metalloendopeptidase activity / mitochondrial inner membrane / electron transfer activity / membrane => GO:0016020 / copper ion binding / heme binding / mitochondrion / proteolysis / metal ion binding
Similarity search - Function
Cytochrome c oxidase subunit 5c / Protein of unknown function DUF1138 / Protein of unknown function (DUF1138) / Predicted cytochrome c oxidase subunit VII / Cytochrome b-c1 complex subunit 8, plants / Cytochrome b-c1 complex subunit 8 / Cytochrome b-c1 complex, subunit 6 / Cytochrome c oxidase, subunit VIa / Cytochrome c oxidase, subunit VIa superfamily / Cytochrome c oxidase subunit VIa ...Cytochrome c oxidase subunit 5c / Protein of unknown function DUF1138 / Protein of unknown function (DUF1138) / Predicted cytochrome c oxidase subunit VII / Cytochrome b-c1 complex subunit 8, plants / Cytochrome b-c1 complex subunit 8 / Cytochrome b-c1 complex, subunit 6 / Cytochrome c oxidase, subunit VIa / Cytochrome c oxidase, subunit VIa superfamily / Cytochrome c oxidase subunit VIa / Cytochrome c oxidase, subunit VIb / Cytochrome c oxidase, subunit VIb superfamily / Cytochrome oxidase c subunit VIb / Cytochrome c oxidase subunit VII / Cytochrome c oxidase subunit VII / Cytochrome c oxidase subunit 2, C-terminal / Cytochrome c oxidase, subunit Vb / Cytochrome c oxidase, subunit Vb superfamily / Cytochrome c oxidase subunit Vb / Cytochrome c oxidase subunit Vb, zinc binding domain profile. / Cytochrome c oxidase, subunit II / Cytochrome C oxidase subunit II, transmembrane domain / Cytochrome b-c1 complex subunit 7 / Cytochrome b-c1 complex subunit 7 superfamily / Ubiquinol-cytochrome C reductase complex 14kD subunit / Cytochrome b-c1 complex subunit 9 / Cytochrome b-c1 complex subunit 8 superfamily / Cytochrome b-c1 complex subunit 9 superfamily / Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like / Cytochrome b-c1 complex subunit Rieske, transmembrane domain / Ubiquinol cytochrome reductase transmembrane region / Ubiquinol-cytochrome C reductase hinge domain / Ubiquinol-cytochrome C reductase hinge domain superfamily / Ubiquinol-cytochrome C reductase hinge protein / Cytochrome c1, transmembrane anchor, C-terminal / Cytochrome C oxidase subunit II, transmembrane domain / Cytochrome oxidase subunit II transmembrane region profile. / Cytochrome c/quinol oxidase subunit II / Ubiquinol-cytochrome c reductase, iron-sulphur subunit / Cytochrome c1 / Cytochrome C1 family / Peptidase M16, zinc-binding site / Insulinase family, zinc-binding region signature. / Copper centre Cu(A) / CO II and nitrous oxide reductase dinuclear copper centers signature. / Cytochrome C oxidase subunit II, transmembrane domain superfamily / Rieske iron-sulphur protein, C-terminal / Cytochrome C oxidase subunit II, periplasmic domain / Rieske iron-sulphur protein / Cytochrome c oxidase subunit II-like C-terminal / Cytochrome oxidase subunit II copper A binding domain profile. / Peptidase M16, C-terminal / Peptidase M16 inactive domain / Peptidase M16, N-terminal / Insulinase (Peptidase family M16) / Metalloenzyme, LuxS/M16 peptidase-like / Rieske [2Fe-2S] iron-sulphur domain / Rieske [2Fe-2S] domain / Rieske [2Fe-2S] iron-sulfur domain profile. / Rieske [2Fe-2S] iron-sulphur domain superfamily / Cytochrome c family profile. / Cytochrome c-like domain / Cytochrome c-like domain superfamily / Cupredoxin
Similarity search - Domain/homology
Cytochrome b-c1 complex subunit Rieske, mitochondrial / uncharacterized protein LOC106757297 / Complex III subunit 9 / uncharacterized protein LOC106759053 / Probable mitochondrial-processing peptidase subunit beta, mitochondrial isoform X1 / Cytochrome c oxidase subunit 2 / cytochrome c oxidase subunit 5b-2, mitochondrial / Cytochrome b-c1 complex subunit 7 / Cytochrome b-c1 complex subunit 8 / cytochrome c oxidase subunit 6a, mitochondrial ...Cytochrome b-c1 complex subunit Rieske, mitochondrial / uncharacterized protein LOC106757297 / Complex III subunit 9 / uncharacterized protein LOC106759053 / Probable mitochondrial-processing peptidase subunit beta, mitochondrial isoform X1 / Cytochrome c oxidase subunit 2 / cytochrome c oxidase subunit 5b-2, mitochondrial / Cytochrome b-c1 complex subunit 7 / Cytochrome b-c1 complex subunit 8 / cytochrome c oxidase subunit 6a, mitochondrial / Cytochrome c oxidase subunit 5C / uncharacterized protein LOC106772338 / Mitochondrial-processing peptidase subunit alpha / Cytochrome b-c1 complex subunit 6 / cytochrome c oxidase subunit 6b-1 / Cytochrome c1-2, heme protein, mitochondrial
Similarity search - Component
Biological speciesVigna radiata (mung bean) / Mung bean (mung bean)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsMaldonado M / Letts JA
CitationJournal: Elife / Year: 2021
Title: Atomic structures of respiratory complex III, complex IV, and supercomplex III-IV from vascular plants.
Authors: Maria Maldonado / Fei Guo / James A Letts /
Abstract: Mitochondrial complex III (CIII) and complex IV (CIV), which can associate into a higher-order supercomplex (SC III+IV), play key roles in respiration. However, structures of these plant complexes ...Mitochondrial complex III (CIII) and complex IV (CIV), which can associate into a higher-order supercomplex (SC III+IV), play key roles in respiration. However, structures of these plant complexes remain unknown. We present atomic models of CIII, CIV, and SC III+IV from determined by single-particle cryoEM. The structures reveal plant-specific differences in the MPP domain of CIII and define the subunit composition of CIV. Conformational heterogeneity analysis of CIII revealed long-range, coordinated movements across the complex, as well as the motion of CIII's iron-sulfur head domain. The CIV structure suggests that, in plants, proton translocation does not occur via the H channel. The supercomplex interface differs significantly from that in yeast and bacteria in its interacting subunits, angle of approach and limited interactions in the mitochondrial matrix. These structures challenge long-standing assumptions about the plant complexes and generate new mechanistic hypotheses.
History
DepositionAug 12, 2020-
Header (metadata) releaseJan 20, 2021-
Map releaseJan 20, 2021-
UpdateFeb 3, 2021-
Current statusFeb 3, 2021Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 6
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 6
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-7jrp
  • Surface level: 6
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_22448.png

Downloads & links

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Map

FileDownload / File: emd_22448.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationPlant mitochondrial supercomplex III2 IV from Vigna radiata. Composite map.
Voxel sizeX=Y=Z: 0.8332 Å
Density
Contour LevelBy AUTHOR: 6.0 / Movie #1: 6
Minimum - Maximum-36.91224 - 45.661976
Average (Standard dev.)0.00015192044 (±1.1589029)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 426.5984 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.833199218750.833199218750.83319921875
M x/y/z512512512
origin x/y/z0.0000.0000.000
length x/y/z426.598426.598426.598
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ512512512
MAP C/R/S321
start NC/NR/NS000
NC/NR/NS512512512
D min/max/mean-36.91245.6620.000

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Supplemental data

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Sample components

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Entire : Mitochondrial Respiratory Complex III2

EntireName: Mitochondrial Respiratory Complex III2
Components
  • Complex: Mitochondrial Respiratory Complex III2
    • Protein or peptide: Mitochondrial-processing peptidase subunit beta, mitochondrial isoform X1
    • Protein or peptide: Alpha-MPP
    • Protein or peptide: COB
    • Protein or peptide: cytochrome c1-2, heme protein, mitochondrial
    • Protein or peptide: Cytochrome b-c1 complex subunit Rieske, mitochondrial
    • Protein or peptide: Cytochrome b-c1 complex subunit 7
    • Protein or peptide: cytochrome b-c1 complex subunit 8
    • Protein or peptide: Cytochrome b-c1 complex subunit 6
    • Protein or peptide: cytochrome b-c1 complex subunit 9
    • Protein or peptide: QCR10
    • Protein or peptide: COX1
    • Protein or peptide: Cytochrome c oxidase subunit 2
    • Protein or peptide: COX3
    • Protein or peptide: COX4
    • Protein or peptide: cytochrome c oxidase subunit 5b-2, mitochondrial
    • Protein or peptide: cytochrome c oxidase subunit 6a, mitochondrial
    • Protein or peptide: cytochrome c oxidase subunit 6b-1
    • Protein or peptide: Cytochrome c oxidase subunit 5C
    • Protein or peptide: COX7a
    • Protein or peptide: COX7c
  • Ligand: ZINC ION
  • Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
  • Ligand: CARDIOLIPIN
  • Ligand: PROTOPORPHYRIN IX CONTAINING FE
  • Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE
  • Ligand: HEME C
  • Ligand: HEME-A
  • Ligand: COPPER (II) ION
  • Ligand: MAGNESIUM ION
  • Ligand: DINUCLEAR COPPER ION
  • Ligand: LYSINE

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Supramolecule #1: Mitochondrial Respiratory Complex III2

SupramoleculeName: Mitochondrial Respiratory Complex III2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#20
Source (natural)Organism: Vigna radiata (mung bean) / Tissue: hypocotyl / Organelle: mitochondria
Molecular weightTheoretical: 485 KDa

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Macromolecule #1: Mitochondrial-processing peptidase subunit beta, mitochondrial is...

MacromoleculeName: Mitochondrial-processing peptidase subunit beta, mitochondrial isoform X1
type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 58.711238 KDa
SequenceString: MSLNRLLSAA RRSDRRASAL SSFRSLSASP ALAPSAASPP APPPPTAMIY DRAAEAIKSK LRQLENPDPR FLKHGNPRPA LSDHTRILA APETRVTTLP NGLRVATESS LAARTATVGV WIDAGSRFET EETNGTAHFL EHMIFKGTER RNARELEEEI E NMGGHLNA ...String:
MSLNRLLSAA RRSDRRASAL SSFRSLSASP ALAPSAASPP APPPPTAMIY DRAAEAIKSK LRQLENPDPR FLKHGNPRPA LSDHTRILA APETRVTTLP NGLRVATESS LAARTATVGV WIDAGSRFET EETNGTAHFL EHMIFKGTER RNARELEEEI E NMGGHLNA YTSREQTTYY AKVTDSDVPQ ALDILADILQ NSRFEENRIS RERDVILREM EEVEGQTEEV IFDHLHATAF QY TPLGRTI LGPAQNIKTI TKAHLQSYIQ THYTAPRMVI AASGAVKHED IVEQVKKLFT KLSTDATTTA QLVAKEPAIF TGS EVRMLD DDIPLAQFAV AFEGASWKDP DSIALMVMQA MLGSWNKTAG GGKHMGSELA QRVGINEVAE SIMAFNTNYK DTGL FGVYA VAKPDCLDDL SYAIMQETTK LVYRVSEDDV TRACNQLKSS LLLHIDGTSP VAEDIGRQLL TYGRRIPFAE LFARI DAVD ASTIKRVANR FIYDKDVAIA AMGPIQRLPD YNWFRRRTYW NRY

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Macromolecule #2: Alpha-MPP

MacromoleculeName: Alpha-MPP / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 54.537684 KDa
SequenceString: MYRAAASSFR RHLKGHGSKL GSTRSSTSAA VAARTSKGGL FSWLTGERSS SLPSLDIPLG GVVLPDPLPD SVEQSKTKIT TLSNGLKIA SETSPNPAAS IGLYLDCGSI YETPFSSGAS HLLERMAFKS TTNRSHFRIV REVEAIGGNI GASASREQMG Y TFDALKTY ...String:
MYRAAASSFR RHLKGHGSKL GSTRSSTSAA VAARTSKGGL FSWLTGERSS SLPSLDIPLG GVVLPDPLPD SVEQSKTKIT TLSNGLKIA SETSPNPAAS IGLYLDCGSI YETPFSSGAS HLLERMAFKS TTNRSHFRIV REVEAIGGNI GASASREQMG Y TFDALKTY VPQMVELLVD CVRNPAFLDW EVNEELRKVK AELGELSNNP QGLLLEAIHS AGYSGALAYP LLAPEAALNR LD GPSLEEF VAENYTAPRM VLAAAGVEHE ELVSIAEPLL SDLPNVPRPD EPKSVYVGGD FRRHGESGGT HVALAFEVPG GWH KEKDAI VLTVLQMLMG GGGSFSAGGP GKGMHSRLYL RVLNEYQQIQ SFSAFNSIFN NTGLFGIYAS TSPDFAPKAV DIAA KELIA IASPGQVTQV QLDRAKKSTK SAVLMNLESR MIASEDIGRQ ILTYGERKPL EQFLKAVDEI TLNDITKISQ KIISS PLTM ASYGDVLSVP SYESVNRKFH AK

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Macromolecule #3: COB

MacromoleculeName: COB / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Vigna radiata (mung bean)
Molecular weightTheoretical: 44.353543 KDa
SequenceString: MTIRNQRFSL LKEPISSTLN QHLIDYPTPS NLSYWWGFGS LAGICLVIQI VTGVFLAMHY TPHVDLAFNS VEHVMRDVEG GWLLRYMHA NGASMFFIVV YLHIFRGLYY ASYSSPREFV WCLGVVIFLL MIVTAFTGYV LPWGQMSFWG ATVITSLASA I PVVGDTIV ...String:
MTIRNQRFSL LKEPISSTLN QHLIDYPTPS NLSYWWGFGS LAGICLVIQI VTGVFLAMHY TPHVDLAFNS VEHVMRDVEG GWLLRYMHA NGASMFFIVV YLHIFRGLYY ASYSSPREFV WCLGVVIFLL MIVTAFTGYV LPWGQMSFWG ATVITSLASA I PVVGDTIV TWLWGGFSVD NATLNRFFSL HYLLPFLLVG ASLLHLAALH QYGSNNPLGV HSEMDQISFY PYFYVKDLVG WV AFAIFFS IWIFYAPNVL GHPDNYIPAN PMSTPPHIVP EWYFLPIYAI LRSIPDKSGG VAAIALVFIC LLALPFFKSM YVR SSSFRP IYQGIFWLLL ADCLLLGWIG CQPVEAPFVT IGQISSFVFF LFFAITPILG RVGRGIPNSY TTDETEM

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Macromolecule #4: cytochrome c1-2, heme protein, mitochondrial

MacromoleculeName: cytochrome c1-2, heme protein, mitochondrial / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 33.556188 KDa
SequenceString: MTGGVFQLLR RKLHPQFTNS SLLSPIIAKK DGAGSTGSRS LKVLALIGAG VSGFFSFSTV AVADEAEHGL ACPSYPWPHK GILSSYDHA SIRRGHQVYT EVCASCHSMS LISYRDLVGV AYTEEEVKAM AAEIEVVDGP NDEGEMFTRP GKLSDRFPQP Y ANEAAARF ...String:
MTGGVFQLLR RKLHPQFTNS SLLSPIIAKK DGAGSTGSRS LKVLALIGAG VSGFFSFSTV AVADEAEHGL ACPSYPWPHK GILSSYDHA SIRRGHQVYT EVCASCHSMS LISYRDLVGV AYTEEEVKAM AAEIEVVDGP NDEGEMFTRP GKLSDRFPQP Y ANEAAARF ANGGAYPPDL SLITKARHNG QNYVFSLLTG YRDPPAGVSI REGLHYNPYF PGGAIAMPKM LNDGAVEYED GT PATEAQM GKDVVSFLSW AAEPEMEERK LMGFKWIFVL SLALLQAAYY RRLRWSVLKS RKLVLDVVN

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Macromolecule #5: Cytochrome b-c1 complex subunit Rieske, mitochondrial

MacromoleculeName: Cytochrome b-c1 complex subunit Rieske, mitochondrial / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO / EC number: quinol-cytochrome-c reductase
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 30.051432 KDa
SequenceString: MLRVAAKRLS SLSSSAWRAN HAASAVLSRN PVAPSLSTEE HRSDPFSLRP EFFLPIRGYA TDSLFHPKEN SLIPEIPATV AAVKNPSSK IIYDEHNHER FPPGDPSKRA FAYFVLTGGR FVYASLIRLL VLKFVLSMSA SKDVLALASL EVDLSSIEPG T TVTVKWRG ...String:
MLRVAAKRLS SLSSSAWRAN HAASAVLSRN PVAPSLSTEE HRSDPFSLRP EFFLPIRGYA TDSLFHPKEN SLIPEIPATV AAVKNPSSK IIYDEHNHER FPPGDPSKRA FAYFVLTGGR FVYASLIRLL VLKFVLSMSA SKDVLALASL EVDLSSIEPG T TVTVKWRG KPVFIRRRTE DDIKLANSVD VASLRDPQQD AERVKNPEWL IVIGVCTHLG CIPLPNAGDF GGWFCPCHGS HY DISGRIR KGPAPYNLEV PTYTFLEENK LMIG

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Macromolecule #6: Cytochrome b-c1 complex subunit 7

MacromoleculeName: Cytochrome b-c1 complex subunit 7 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 14.392698 KDa
SequenceString:
MASFLQSFLD PKKNWLAAQH MKSLVKRLSK YGLRYDDLYD PYYDLDVKEA LNRLPKEVVD ARHARLKRAI DLSMKHEYLP EDLQAMQTP FRGYLQDMLA LVKRERAERE ALGGLPLYQR SIP

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Macromolecule #7: cytochrome b-c1 complex subunit 8

MacromoleculeName: cytochrome b-c1 complex subunit 8 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 8.349831 KDa
SequenceString:
MGKQIVPVKS VIYALSPFQQ KVMTGLWKDL PTKIHHKVSE NWISATLLLG PLVGTYAYVQ NYLEKEKLHH RY

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Macromolecule #8: Cytochrome b-c1 complex subunit 6

MacromoleculeName: Cytochrome b-c1 complex subunit 6 / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 8.117424 KDa
SequenceString:
MADEEPVDQK RYLEESCKPK CVKPLLEYQA CIKRIHGDDS GQKHCTGQYF DYWFCVDKCV APKLFTKLK

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Macromolecule #9: cytochrome b-c1 complex subunit 9

MacromoleculeName: cytochrome b-c1 complex subunit 9 / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 8.20342 KDa
SequenceString:
METVARRRGG GIFESLYKVV MRRNSVYVTF VIAGAFLGER AVDYGIHKLW EANNVGKRYE DIPVLGQKLS EE

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Macromolecule #10: QCR10

MacromoleculeName: QCR10 / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Vigna radiata (mung bean)
Molecular weightTheoretical: 8.948875 KDa
SequenceString:
MAGLPARLRI QPADVKAAAM WGVAAATGGL YLVQVSILVL PPVKVVFHFY LVSGFRICLD VKDLRTMPCS APRIWRLYIL I

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Macromolecule #11: COX1

MacromoleculeName: COX1 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Vigna radiata (mung bean)
Molecular weightTheoretical: 57.960559 KDa
SequenceString: MTNPVRWLFS TNHKDIGTLY FIFGAIAGVM GTCFSVLIRM ELARPGDQIL GGNHQLYNVL ITAHAFLMIF FMVMPAMIGG FGNWFVPIL IGAPDMAFPR LNNISFWLLP PSLLLLLSSA LVEVGSGTGW TVYPPLSGIT SHSGGAVDLA IFSLHLSGVS S ILGSINFI ...String:
MTNPVRWLFS TNHKDIGTLY FIFGAIAGVM GTCFSVLIRM ELARPGDQIL GGNHQLYNVL ITAHAFLMIF FMVMPAMIGG FGNWFVPIL IGAPDMAFPR LNNISFWLLP PSLLLLLSSA LVEVGSGTGW TVYPPLSGIT SHSGGAVDLA IFSLHLSGVS S ILGSINFI TTIFNMRGPG MTMHRSPLFV WSVLVTAFLL LLSLPVLAGA ITMLLTDRNF NTTFFDPAGG GDPILYQHLF WF FGHPEVY ILILPGFGII SHIVSTFSGK PVFGYLGMVY AMISIGVLGF LVWAHHMFTV GLDVDTRAYF TAATMIIAVP TGI KIFSWI ATMWGGSIQY KTPMLFAVGF IFLFTIGGLT GIVLANSGLD IALHDTYYVV AHFHYVLSMG AVFALFAGFY YWVG KIFGR TYPETLGQIH FWITFFGVNL TFFPMHFLGL SGMPRRIPDY PDAYAGWNAL SSFGSYISVV GICCFFVVVT ITSTS GNNI TRANIPWAVE QNSTTLEWLV QSPPAFHTFG ELPAIKETKS YVK

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Macromolecule #12: Cytochrome c oxidase subunit 2

MacromoleculeName: Cytochrome c oxidase subunit 2 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 28.391602 KDa
SequenceString: MCGGFPAISF CDAPEPWQLG FQDAATPIMQ GIMDLHHDIF FFLVQIAVFV LWVLSRALWC FRSKISPIPQ RIVHGTTIEI LWTIFPSII LMFIAIPSFT LLYSMDDVVV DPAITIKAIG HQWYWSYEYS DYNNSDEQSL AFDSYMVPED DLELGQLRLL E VDNRVVVP ...String:
MCGGFPAISF CDAPEPWQLG FQDAATPIMQ GIMDLHHDIF FFLVQIAVFV LWVLSRALWC FRSKISPIPQ RIVHGTTIEI LWTIFPSII LMFIAIPSFT LLYSMDDVVV DPAITIKAIG HQWYWSYEYS DYNNSDEQSL AFDSYMVPED DLELGQLRLL E VDNRVVVP AKTHLRVLIT SADVLHSWAV PSLGVKCDAV PGRLNQISTF IQREGVYYGQ CSEICGTNHA FMPIVVEAVS TK DYGSWVS NQIQ

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Macromolecule #13: COX3

MacromoleculeName: COX3 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Vigna radiata (mung bean)
Molecular weightTheoretical: 29.967893 KDa
SequenceString: MIESQRHSYH LVDPSPWPIS GSLGALATTV GGVMYMHSFQ GGATLLSLGL IFILYTMFVW WRDVLRESTL EGHHTKVVQL GLRYGFILF IVSEVMFLFA FFWAFFHSSL APTVEIGGIW PPLGIWVLDP WEIPFLNTLI LLSSGAAVTW AHHAILAGKE K RAVYALVA ...String:
MIESQRHSYH LVDPSPWPIS GSLGALATTV GGVMYMHSFQ GGATLLSLGL IFILYTMFVW WRDVLRESTL EGHHTKVVQL GLRYGFILF IVSEVMFLFA FFWAFFHSSL APTVEIGGIW PPLGIWVLDP WEIPFLNTLI LLSSGAAVTW AHHAILAGKE K RAVYALVA TVLLALVFTG FQGMEYYQAP FTISDSIYGS TFFLATGFHG FHVIIGTLFL IICGIRQYLG HLTKEHHVGF EA AAWYWHF VDVVWLFLFV SIYWWGGI

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Macromolecule #14: COX4

MacromoleculeName: COX4 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 8.81102 KDa
SequenceString:
MSTKYIVSAI LGSFGIAWVC DYYVSEKKIF GGSTPGTITN KEWGEETDKK FQAWPRTAGP PVVVNPITRQ NFVVKSRSE

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Macromolecule #15: cytochrome c oxidase subunit 5b-2, mitochondrial

MacromoleculeName: cytochrome c oxidase subunit 5b-2, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 16.694705 KDa
SequenceString:
MLRRFLSHSN LRSLHTALSP SRPRFAAPLL TRHVTAQSGA SSVRKRVEDV VPIATGHERE EIQASLEGRD ILEINHPEGP FGTKEAPAI VKSYFDRRIV GCPGGEGEDE HDVVWFWLEN GKPHECPVCS QYFELKVVGP GGDPYGHGDH H

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Macromolecule #16: cytochrome c oxidase subunit 6a, mitochondrial

MacromoleculeName: cytochrome c oxidase subunit 6a, mitochondrial / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 11.225671 KDa
SequenceString:
MATSLVRSGF LRTALRGGAR GSQVPKRNFS SAGHHDDAYE TAKWEKITYL GIVSCTGLAI YNLSKGHPHT EEPPAYPYLH IRNKEFPWG PDGLFETKKH H

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Macromolecule #17: cytochrome c oxidase subunit 6b-1

MacromoleculeName: cytochrome c oxidase subunit 6b-1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 20.018635 KDa
SequenceString:
MAETAANKSQ SLAEEYHVSD KQEKTAVVEK PVEVKEVENP QEAGSVEAVV EKIVEETTPV APAVAQESSE VSPPPAEESA EESTEEQSS GNVEDNSGNE DAAEKTPEIK LETAPADFRF PTTNQTRHCF TRYIEYHRCV AAKGEGASEC DKFAKYYRSL C PGEWIDRW NEQRENGTFP GPL

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Macromolecule #18: Cytochrome c oxidase subunit 5C

MacromoleculeName: Cytochrome c oxidase subunit 5C / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 7.120326 KDa
SequenceString:
MAGPRIAHAT LKGPSVVKEI IIGITLGLAA GSVWKMHHWN EQRKIRTFYD LLEKGEIGVV VDEQ

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Macromolecule #19: COX7a

MacromoleculeName: COX7a / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 7.622931 KDa
SequenceString:
MSEPPFRPRE KLIEKQKHFQ SVHKHTYLKG PYDKITSVAI PLALAASSLY LIGRGIYNMS HGIGKKE

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Macromolecule #20: COX7c

MacromoleculeName: COX7c / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mung bean (mung bean)
Molecular weightTheoretical: 10.505158 KDa
SequenceString:
MAFNNALRSA AKLIASSESS ISTSVSRGFH STPMKRMGGG HGHDEPYYIH AKHMYNLDRM KHRGLKMSLA VFSAFSIGVA VPVYAVIFQ QKKTASA

+
Macromolecule #21: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 21 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #22: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

MacromoleculeName: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 22 / Number of copies: 5 / Formula: PC1
Molecular weightTheoretical: 790.145 Da
Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM / Phosphatidylcholine

+
Macromolecule #23: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 23 / Number of copies: 9 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

+
Macromolecule #24: PROTOPORPHYRIN IX CONTAINING FE

MacromoleculeName: PROTOPORPHYRIN IX CONTAINING FE / type: ligand / ID: 24 / Number of copies: 4 / Formula: HEM
Molecular weightTheoretical: 616.487 Da
Chemical component information

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

+
Macromolecule #25: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE

MacromoleculeName: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE / type: ligand / ID: 25 / Number of copies: 29 / Formula: 3PE
Molecular weightTheoretical: 748.065 Da

+
Macromolecule #26: HEME C

MacromoleculeName: HEME C / type: ligand / ID: 26 / Number of copies: 2 / Formula: HEC
Molecular weightTheoretical: 618.503 Da
Chemical component information

ChemComp-HEC:
HEME C / Heme C

+
Macromolecule #27: HEME-A

MacromoleculeName: HEME-A / type: ligand / ID: 27 / Number of copies: 2 / Formula: HEA
Molecular weightTheoretical: 852.837 Da
Chemical component information

ChemComp-HEA:
HEME-A / Heme A

+
Macromolecule #28: COPPER (II) ION

MacromoleculeName: COPPER (II) ION / type: ligand / ID: 28 / Number of copies: 1 / Formula: CU
Molecular weightTheoretical: 63.546 Da
Chemical component information

ChemComp-CU:
COPPER (II) ION / Copper

+
Macromolecule #29: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 29 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #30: DINUCLEAR COPPER ION

MacromoleculeName: DINUCLEAR COPPER ION / type: ligand / ID: 30 / Number of copies: 1 / Formula: CUA
Molecular weightTheoretical: 127.092 Da
Chemical component information

ChemComp-CUA:
DINUCLEAR COPPER ION

+
Macromolecule #31: LYSINE

MacromoleculeName: LYSINE / type: ligand / ID: 31 / Number of copies: 1 / Formula: LYS
Molecular weightTheoretical: 147.195 Da
Chemical component information

ChemComp-LYS:
LYSINE / Lysine

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

Concentration6 mg/mL
BufferpH: 7.8
Component:
ConcentrationNameFormula
20.0 mMHEPES
150.0 mMsodium chlorideNaClSodium chloride
1.0 mMEDTAEthylenediaminetetraacetic acid
0.2 %digitonin
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 288 K / Instrument: FEI VITROBOT MARK III
DetailsThis sample was monodisperse on size exclusion chromatography but was a mixture of different mitochondrial respiratory complexes

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated defocus max: 3.0 µm / Calibrated defocus min: 1.5 µm / Calibrated magnification: 60010 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Number grids imaged: 1 / Number real images: 9816 / Average exposure time: 3.0 sec. / Average electron dose: 86.4 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Particle selectionNumber selected: 150000000
CTF correctionSoftware - Name: CTFFIND (ver. 4)
Startup modelType of model: NONE / Details: Ab initio model generation
Initial angle assignmentType: OTHER / Software - Name: cryoSPARC / Details: Stochastic gradient descent
Final angle assignmentType: OTHER / Software - Name: cryoSPARC / Details: Stochastic gradient descent
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 29348

-
Atomic model buiding 1

Initial model(PDB ID:

6q9e

,

6hu9

)
RefinementSpace: REAL / Protocol: AB INITIO MODEL / Overall B value: 67 / Target criteria: correlation coefficient
Output model

PDB-7jrp:
Plant Mitochondrial complex SC III2+IV from Vigna radiata

+
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