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基本情報
登録情報 | ![]() | |||||||||||||||||||||
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タイトル | Structure of bacterial ribosome determined by cryoEM at 100 keV | |||||||||||||||||||||
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![]() | RIBOSOME | |||||||||||||||||||||
機能・相同性 | ![]() negative regulation of cytoplasmic translational initiation / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / positive regulation of ribosome biogenesis ...negative regulation of cytoplasmic translational initiation / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / positive regulation of ribosome biogenesis / negative regulation of cytoplasmic translation / four-way junction DNA binding / DnaA-L2 complex / translation repressor activity / negative regulation of translational initiation / negative regulation of DNA-templated DNA replication initiation / regulation of mRNA stability / mRNA regulatory element binding translation repressor activity / ribosome assembly / positive regulation of RNA splicing / assembly of large subunit precursor of preribosome / transcription elongation factor complex / cytosolic ribosome assembly / regulation of DNA-templated transcription elongation / DNA endonuclease activity / ribosomal large subunit assembly / transcription antitermination / response to reactive oxygen species / translational initiation / regulation of cell growth / DNA-templated transcription termination / maintenance of translational fidelity / response to radiation / mRNA 5'-UTR binding / large ribosomal subunit / ribosome biogenesis / ribosome binding / regulation of translation / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / transferase activity / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / molecular adaptor activity / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / translation / response to antibiotic / negative regulation of DNA-templated transcription / mRNA binding / DNA binding / RNA binding / zinc ion binding / membrane / cytoplasm / cytosol 類似検索 - 分子機能 | |||||||||||||||||||||
生物種 | ![]() ![]() | |||||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.5 Å | |||||||||||||||||||||
![]() | McMullan G / Naydenova K / Mihaylov D / Peet MJ / Wilson H / Yamashita K / Dickerson JL / Chen S / Cannone G / Lee Y ...McMullan G / Naydenova K / Mihaylov D / Peet MJ / Wilson H / Yamashita K / Dickerson JL / Chen S / Cannone G / Lee Y / Hutchings KA / Gittins O / Sobhy M / Wells T / El-Gomati MM / Dalby J / Meffert M / Schulze-Briese C / Henderson R / Russo CJ | |||||||||||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structure determination by cryoEM at 100 keV. 著者: Greg McMullan / Katerina Naydenova / Daniel Mihaylov / Keitaro Yamashita / Mathew J Peet / Hugh Wilson / Joshua L Dickerson / Shaoxia Chen / Giuseppe Cannone / Yang Lee / Katherine A ...著者: Greg McMullan / Katerina Naydenova / Daniel Mihaylov / Keitaro Yamashita / Mathew J Peet / Hugh Wilson / Joshua L Dickerson / Shaoxia Chen / Giuseppe Cannone / Yang Lee / Katherine A Hutchings / Olivia Gittins / Mohamed A Sobhy / Torquil Wells / Mohamed M El-Gomati / Jason Dalby / Matthias Meffert / Clemens Schulze-Briese / Richard Henderson / Christopher J Russo / ![]() ![]() ![]() 要旨: Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, specimen preparation difficulties, and cost. We describe a purpose- ...Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, specimen preparation difficulties, and cost. We describe a purpose-built instrument operating at 100 keV-including advances in electron optics, detection, and processing-that makes structure determination fast and simple at a fraction of current costs. The instrument attains its theoretical performance limits, allowing atomic resolution imaging of gold test specimens and biological molecular structure determination in hours. We demonstrate its capabilities by determining the structures of eleven different specimens, ranging in size from 140 kDa to 2 MDa, using a fraction of the data normally required. CryoEM with a microscope designed specifically for high-efficiency, on-the-spot imaging of biological molecules will expand structural biology to a wide range of previously intractable problems. | |||||||||||||||||||||
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構造の表示
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マップデータ | ![]() | 31.2 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 73.5 KB 73.5 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 14.3 KB | 表示 | ![]() |
画像 | ![]() | 126.5 KB | ||
マスクデータ | ![]() | 244.1 MB | ![]() | |
Filedesc metadata | ![]() | 14.8 KB | ||
その他 | ![]() ![]() | 194.3 MB 194.1 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8pvaMC ![]() 8pv9C ![]() 8pvbC ![]() 8pvcC ![]() 8pvdC ![]() 8pveC ![]() 8pvfC ![]() 8pvgC ![]() 8pvhC ![]() 8pviC ![]() 8pvjC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.031 Å | ||||||||||||||||||||
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-マスク #1
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #2
ファイル | emd_17959_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_17959_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : E. coli 70S ribosome
+超分子 #1: E. coli 70S ribosome
+分子 #1: 16S rRNA
+分子 #22: 23S rRNA
+分子 #23: 5S rRNA
+分子 #53: mRNA
+分子 #54: A-site tRNA-val
+分子 #55: P-site tRNA-fMet
+分子 #56: E-site tRNA
+分子 #2: Small ribosomal subunit protein uS2
+分子 #3: Small ribosomal subunit protein uS3
+分子 #4: Small ribosomal subunit protein uS4
+分子 #5: Small ribosomal subunit protein uS5
+分子 #6: Small ribosomal subunit protein bS6, fully modified isoform
+分子 #7: Small ribosomal subunit protein uS7
+分子 #8: Small ribosomal subunit protein uS8
+分子 #9: Small ribosomal subunit protein uS9
+分子 #10: Small ribosomal subunit protein uS10
+分子 #11: Small ribosomal subunit protein uS11
+分子 #12: Small ribosomal subunit protein uS12
+分子 #13: Small ribosomal subunit protein uS13
+分子 #14: Small ribosomal subunit protein uS14
+分子 #15: Small ribosomal subunit protein uS15
+分子 #16: Small ribosomal subunit protein bS16
+分子 #17: Small ribosomal subunit protein uS17
+分子 #18: Small ribosomal subunit protein bS18
+分子 #19: Small ribosomal subunit protein uS19
+分子 #20: Small ribosomal subunit protein bS20
+分子 #21: Small ribosomal subunit protein bS21
+分子 #24: Large ribosomal subunit protein uL2
+分子 #25: Large ribosomal subunit protein uL3
+分子 #26: Large ribosomal subunit protein uL4
+分子 #27: Large ribosomal subunit protein uL5
+分子 #28: Large ribosomal subunit protein uL6
+分子 #29: Large ribosomal subunit protein bL9
+分子 #30: Large ribosomal subunit protein uL13
+分子 #31: 50S ribosomal protein L14
+分子 #32: 50S ribosomal protein L15
+分子 #33: Large ribosomal subunit protein uL16
+分子 #34: 50S ribosomal protein L17
+分子 #35: 50S ribosomal protein L18
+分子 #36: 50S ribosomal protein L19
+分子 #37: 50S ribosomal protein L20
+分子 #38: 50S ribosomal protein L21
+分子 #39: 50S ribosomal protein L22
+分子 #40: 50S ribosomal protein L23
+分子 #41: 50S ribosomal protein L24
+分子 #42: 50S ribosomal protein L25
+分子 #43: 50S ribosomal protein L27
+分子 #44: 50S ribosomal protein L28
+分子 #45: 50S ribosomal protein L29
+分子 #46: 50S ribosomal protein L30
+分子 #47: 50S ribosomal protein L32
+分子 #48: 50S ribosomal protein L33
+分子 #49: 50S ribosomal protein L34
+分子 #50: 50S ribosomal protein L35
+分子 #51: 50S ribosomal protein L36
+分子 #52: Large ribosomal subunit protein bL31A
+分子 #57: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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グリッド | モデル: UltrAuFoil R0./1 / 材質: GOLD / メッシュ: 300 |
凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | JEOL 1400/HR + YPS FEG |
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撮影 | フィルム・検出器のモデル: DECTRIS SINGLA (1k x 1k) デジタル化 - サイズ - 横: 1030 pixel / デジタル化 - サイズ - 縦: 1066 pixel / 平均電子線量: 24.0 e/Å2 |
電子線 | 加速電圧: 100 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 3.0 µm / 最小 デフォーカス(公称値): 0.5 µm |